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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6.Cg-an/BrkJ (Changed: 20-JUL-10 ) B6.Cg-an/Brk (Changed: 29-AUG-07 ) C57BL/6J-an (Changed: 17-FEB-05 ) Type Congenic; Mutant Strain; Radiation Induced Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N96pN1
Generation Definitions
Related Strains
Strains carrying Cdk5rap2an allele
002307 WB.Cg-Cdk5rap2an/BrkJ View Strains carrying Cdk5rap2an (1 strain)
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Microcephaly 3, Primary, Autosomal Recessive; MCPH3 (CDK5RAP2)
assigned by genotype
Cdk5rap2an/Cdk5rap2an
B6.Cg-Cdk5rap2an
- mortality/aging
- premature death
- die within one week of age (MGI Ref ID J:160533)
- growth/size phenotype
- decreased body size
- nervous system phenotype
- abnormal cerebral cortex morphology
- disproportionately reduced cortex (MGI Ref ID J:160533)
- abnormal embryonic/fetal subventricular zone morphology
- reduced ganglionic eminence (MGI Ref ID J:160533)
- abnormal forebrain development
- profound reduction of basal progenitors at later stages in neurogenesis (MGI Ref ID J:160533)
- abnormal neuron proliferation
- cortical neurons divide less (MGI Ref ID J:160533)
- decreased brain size
- enlarged brain ventricle
- enlarged ventricle (MGI Ref ID J:160533)
- increased neuron apoptosis
- premature cell cycle exit and increased cell death (MGI Ref ID J:160533)
- small hippocampus
- at P0 the hippocampus is smaller than wild-type (MGI Ref ID J:160533)
- small olfactory bulb (MGI Ref ID J:160533)
- cellular phenotype
- abnormal mitosis (MGI Ref ID J:160533)
- abnormal mitotic spindle morphology
- defective mitotic spindle orientation (MGI Ref ID J:160533)
- abnormal neuron proliferation
- cortical neurons divide less (MGI Ref ID J:160533)
- increased neuron apoptosis
- premature cell cycle exit and increased cell death (MGI Ref ID J:160533)
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Cdk5rap2an/Cdk5rap2an
Background Not Specified
- mortality/aging
- postnatal lethality
- growth/size phenotype
- decreased body size (MGI Ref ID J:208)
- hematopoietic system phenotype
- abnormal erythropoiesis
- decreased leukocyte cell number (MGI Ref ID J:5439)
- immune system phenotype
- decreased leukocyte cell number (MGI Ref ID J:5439)
- reproductive system phenotype
- abnormal spermatogenesis
- almost no spermatogenesis is seen in adult testes (MGI Ref ID J:22618)
- decreased germ cell number
- reduced number of germ cells starting at E12-E13 (MGI Ref ID J:15165)
- decreased oocyte number (MGI Ref ID J:15165)
- reduced fertility (MGI Ref ID J:5439)
- survivors are almost completely sterile (MGI Ref ID J:22618)
- tumorigenesis
- increased adenoma incidence
- eventually ovaries become tubular adenomas (MGI Ref ID J:15165)
- vision/eye phenotype
- abnormal eye morphology
- older survivors (beyond 6-8 months) frequently develop eye opacities (MGI Ref ID J:22618)
- skeleton phenotype
- kyphosis
- older survivors (6-8 months) become kyphotic (MGI Ref ID J:22618)
- integument phenotype
- premature hair loss
- older survivors (6-8 months) lose their hair completely (MGI Ref ID J:22618)
Cdk5rap2an/Cdk5rap2an
involves: C57BL/6 * WB
- nervous system phenotype
- abnormal cerebral cortex morphology
- cortical hypoplasia visualized by shortening of the forebrain (MGI Ref ID J:160533)
This mouse can be used to support research in many areas including:Cdk5rap2an related
Cancer Research
Increased Tumor Incidence
Hematological Research
Anemia, Iron Homeostasis Defects
Hematopoietic Defects
Research Tools
Genetics Research
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Cdk5rap2an | ||
|---|---|---|---|
| Allele Name | Hertwig's anemia | ||
| Allele Type | Radiation induced | ||
| Gene Symbol and Name | Cdk5rap2, CDK5 regulatory subunit associated protein 2 | ||
| Chromosome | 4 | ||
| Gene Common Name(s) | 2900018K03Rik; C48; Cep215; Hertwig's anemia; MCPH3; MGC:38528; RIKEN cDNA 2900018K03 gene; an; mKIAA1633; | ||
| Molecular Note | Exon 4 is inverted which results in an in-frame 111 bp deletion with the loss of exon 4 from the mRNA. Northern analysis demonstrated that exon 4 was uniformly omitted from the mutant mRNA. [MGI Ref ID J:160533] | ||
References
References provided by MGI
Selected Reference(s)
Barker JE; Bernstein SE. 1983. Hertwig's anemia: characterization of the stem cell defect. Blood 61(4):765-9. [PubMed: 6831040] [MGI Ref ID J:7003]
Barker JE; Deveau SA; Compton ST; Fancher K; Eppig JT. 2005. High incidence, early onset of histiocytic sarcomas in mice with Hertwig's anemia. Exp Hematol 33(10):1118-29. [PubMed: 16219534] [MGI Ref ID J:102334]
Eppig JT; Barker JE. 1984. Chromosome abnormalities in mice with Hertwig's anemia. Blood 64(3):727-32. [PubMed: 6466874] [MGI Ref ID J:7537]
Hertwig P. 1942. Neue Mutationen und Koppelungsgruppen bei der Hausmaus Z Indukt Abstamm Vererbungsl 80:220-246. [MGI Ref ID J:208]
Russell ES; McFarland EC; Peters H. 1985. Gametic and pleiotropic defects in mouse fetuses with Hertwig's macrocytic anemia. Dev Biol 110(2):331-7. [PubMed: 4018402] [MGI Ref ID J:7931]
Taney FH; Vasilenko P; Steinetz BG; Weiss G; Cole D; Goldsmith LT. 1991. The role of estrogen and relaxin in the reproductive abnormalities of mice with Hertwig's anemia. Biol Reprod 45(5):719-26. [PubMed: 1756209] [MGI Ref ID J:753]
Additional References
Cdk5rap2an relatedBannerman RM; Edwards JA; Pinkerton PH. 1973. Hereditary disorders of the red cell in animals. Prog Hematol 8:131-79. [PubMed: 4596202] [MGI Ref ID J:5439]
Barker JE. 1995. Murine Hertwig's anemia: premature death after normal bone marrow transplantation is radiation dose-dependent. Blood 85(9):2627-31. [PubMed: 7727788] [MGI Ref ID J:26056]
Bernstein SE. 1975. Bone marrow implantation therapy in Hertwig's anemic mice. Jackson Lab Ann Rep 46:87. [MGI Ref ID J:13371]
Bernstein SE. 1969. Hereditary disorders of the rodent erythron. In: Genetics in Laboratory Animal Medicine. Natl Acad Sci Publ, Washington, DC. [MGI Ref ID J:30699]
Harrison DE. 1976. Stem cell multiplication rate in W-anemic mice, and immune response of mice with Hertwig's anemia. Jackson Lab Ann Rep 47:59. [MGI Ref ID J:13372]
Lizarraga SB; Margossian SP; Harris MH; Campagna DR; Han AP; Blevins S; Mudbhary R; Barker JE; Walsh CA; Fleming MD. 2010. Cdk5rap2 regulates centrosome function and chromosome segregation in neuronal progenitors. Development 137(11):1907-17. [PubMed: 20460369] [MGI Ref ID J:160533]
Russell ES. 1970. Abnormalities of erythropoiesis associated with mutant genes in mice. In: Regulation of Hematopoiesis. Appleton-Century-Crofts, New York. [MGI Ref ID J:27511]
Russell ES; Bernstein SE. 1966. Blood and Blood Formation. In: Biology of the Laboratory Mouse. McGraw Hill, New York. [MGI Ref ID J:24829]
Russell ES; Meier H. 1966. Constitutional Diseases. In: Biology of the Laboratory Mouse. McGraw - Hill, New York. [MGI Ref ID J:22618]
Russell ES; Whitten WK; Beamer WG; McFarland EC; Peters H. 1976. Germ cell defect in Hertwig's anemic mice Jackson Lab Ann Rep 47:89. [MGI Ref ID J:15165]
Sassa S; Bernstein SE. 1978. Studies of erythrocyte protoporphyrin in anemic mutant mice: use of a modified hematofluorometer for the detection of heterozygotes for hemolytic disease. Exp Hematol 6(5):479-87. [PubMed: 658175] [MGI Ref ID J:5985]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery of Strains Needing Progeny Testing.
At least two untested males and two untested females (two pairs) will be recovered (eight or more mice is typical). The total number of animals provided, their gender and genotype will vary. Untested animals typically are available to ship between 13 and 16 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery will be done, extending the overall recovery time to approximately 25 weeks.Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 (from U.S.A., Canada and Puerto Rico only) or 1-207-288-5845 (from any location). The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery of Strains Needing Progeny Testing.
At least two untested males and two untested females (two pairs) will be recovered (eight or more mice is typical). The total number of animals provided, their gender and genotype will vary. Untested animals typically are available to ship between 13 and 16 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery will be done, extending the overall recovery time to approximately 25 weeks.Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 (from U.S.A., Canada and Puerto Rico only) or 1-207-288-5845 (from any location). The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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