Strain Name:

WB.Cg-Cdk5rap2an/BrkJ

Stock Number:

002307

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names WB.Cg-an/BrkJ    (Changed: 20-JUL-10 )
WB.Cg-an/Brk    (Changed: 29-AUG-07 )
WB/Re-an    (Changed: 17-FEB-05 )
Type Congenic; Mutant Strain; Radiation Induced Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse

Related Strains

Strains carrying   Cdk5rap2an allele
002306   B6.Cg-Cdk5rap2an/BrkJ
View Strains carrying   Cdk5rap2an     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Microcephaly 3, Primary, Autosomal Recessive; MCPH3   (CDK5RAP2)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Cdk5rap2an/Cdk5rap2an

        Background Not Specified
  • mortality/aging
  • postnatal lethality
    • animals die a few days after birth   (MGI Ref ID J:208)
    • most die within a few days or weeks of birth, however a few reach adulthood   (MGI Ref ID J:5439)
  • growth/size/body phenotype
  • decreased body size   (MGI Ref ID J:208)
  • hematopoietic system phenotype
  • abnormal erythropoiesis
    • erythropoiesis is decreased in the liver   (MGI Ref ID J:5439)
    • complete regression of hepatic hemopoiesis occurs at about 4 days after birth compared to 10 days in controls   (MGI Ref ID J:5439)
    • abnormal erythrocyte morphology
      • mean potential life span of erythrocytes is reduced   (MGI Ref ID J:5439)
      • anemia
        • anemic at birth   (MGI Ref ID J:208)
        • macrocytic anemia   (MGI Ref ID J:5439)
      • anisocytosis   (MGI Ref ID J:5439)
      • macrocytosis
        • seen at birth   (MGI Ref ID J:5439)
    • reticulocytopenia   (MGI Ref ID J:5439)
  • decreased leukocyte cell number   (MGI Ref ID J:5439)
  • immune system phenotype
  • decreased leukocyte cell number   (MGI Ref ID J:5439)
  • reproductive system phenotype
  • abnormal spermatogenesis
    • almost no spermatogenesis is seen in adult testes   (MGI Ref ID J:22618)
  • decreased germ cell number
    • reduced number of germ cells starting at E12-E13   (MGI Ref ID J:15165)
    • decreased oocyte number   (MGI Ref ID J:15165)
  • reduced fertility   (MGI Ref ID J:5439)
    • survivors are almost completely sterile   (MGI Ref ID J:22618)
  • tumorigenesis
  • increased adenoma incidence
    • eventually ovaries become tubular adenomas   (MGI Ref ID J:15165)
  • vision/eye phenotype
  • abnormal eye morphology
    • older survivors (beyond 6-8 months) frequently develop eye opacities   (MGI Ref ID J:22618)
  • skeleton phenotype
  • kyphosis
    • older survivors (6-8 months) become kyphotic   (MGI Ref ID J:22618)
  • integument phenotype
  • premature hair loss
    • older survivors (6-8 months) lose their hair completely   (MGI Ref ID J:22618)

Cdk5rap2an/Cdk5rap2an

        B6.Cg-Cdk5rap2an
  • mortality/aging
  • premature death
    • die within one week of age   (MGI Ref ID J:160533)
  • growth/size/body phenotype
  • decreased body size
    • reduced body size at E12.5   (MGI Ref ID J:160533)
    • runted   (MGI Ref ID J:160533)
  • nervous system phenotype
  • abnormal cerebral cortex morphology
    • disproportionately reduced cortex   (MGI Ref ID J:160533)
    • loss of cortex neurons
      • have fewer total neurons and the last-born superficial neurons are particularly reduced   (MGI Ref ID J:160533)
    • thin cerebral cortex   (MGI Ref ID J:160533)
  • abnormal embryonic/fetal subventricular zone morphology
    • reduced ganglionic eminence   (MGI Ref ID J:160533)
  • abnormal forebrain development
    • profound reduction of basal progenitors at later stages in neurogenesis   (MGI Ref ID J:160533)
  • abnormal neuron proliferation
    • cortical neurons divide less   (MGI Ref ID J:160533)
  • decreased brain size
    • reduced brain size at E12.5,   (MGI Ref ID J:160533)
    • brain-to-body weight ratio at P0 is decreased   (MGI Ref ID J:160533)
  • enlarged brain ventricles
    • enlarged ventricle   (MGI Ref ID J:160533)
  • increased neuron apoptosis
    • premature cell cycle exit and increased cell death   (MGI Ref ID J:160533)
  • small hippocampus
    • at P0 the hippocampus is smaller than wild-type   (MGI Ref ID J:160533)
  • small olfactory bulb   (MGI Ref ID J:160533)
  • cellular phenotype
  • abnormal mitosis   (MGI Ref ID J:160533)
    • abnormal mitotic spindle morphology
      • defective mitotic spindle orientation   (MGI Ref ID J:160533)
  • abnormal neuron proliferation
    • cortical neurons divide less   (MGI Ref ID J:160533)
  • increased neuron apoptosis
    • premature cell cycle exit and increased cell death   (MGI Ref ID J:160533)

Cdk5rap2an/Cdk5rap2an

        involves: C57BL/6 * WB
  • nervous system phenotype
  • abnormal cerebral cortex morphology
    • cortical hypoplasia visualized by shortening of the forebrain   (MGI Ref ID J:160533)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cdk5rap2an related

Cancer Research
Increased Tumor Incidence

Hematological Research
Anemia, Iron Homeostasis Defects
Hematopoietic Defects

Reproductive Biology Research

Research Tools
Genetics Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Cdk5rap2an
Allele Name Hertwig's anemia
Allele Type Radiation induced
Gene Symbol and Name Cdk5rap2, CDK5 regulatory subunit associated protein 2
Chromosome 4
Gene Common Name(s) 2900018K03Rik; C48; Cep215; Hertwig's anemia; MCPH3; MGC:38528; RIKEN cDNA 2900018K03 gene; an; mKIAA1633;
Molecular Note Exon 4 is inverted which results in an in-frame 111 bp deletion with the loss of exon 4 from the mRNA. Northern analysis demonstrated that exon 4 was uniformly omitted from the mutant mRNA. [MGI Ref ID J:160533]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Barker JE; Bernstein SE. 1983. Hertwig's anemia: characterization of the stem cell defect. Blood 61(4):765-9. [PubMed: 6831040]  [MGI Ref ID J:7003]

Barker JE; Deveau SA; Compton ST; Fancher K; Eppig JT. 2005. High incidence, early onset of histiocytic sarcomas in mice with Hertwig's anemia. Exp Hematol 33(10):1118-29. [PubMed: 16219534]  [MGI Ref ID J:102334]

Eppig JT; Barker JE. 1984. Chromosome abnormalities in mice with Hertwig's anemia. Blood 64(3):727-32. [PubMed: 6466874]  [MGI Ref ID J:7537]

Hertwig P. 1942. Neue Mutationen und Koppelungsgruppen bei der Hausmaus Z Indukt Abstamm Vererbungsl 80:220-246.  [MGI Ref ID J:208]

Russell ES; McFarland EC; Peters H. 1985. Gametic and pleiotropic defects in mouse fetuses with Hertwig's macrocytic anemia. Dev Biol 110(2):331-7. [PubMed: 4018402]  [MGI Ref ID J:7931]

Taney FH; Vasilenko P; Steinetz BG; Weiss G; Cole D; Goldsmith LT. 1991. The role of estrogen and relaxin in the reproductive abnormalities of mice with Hertwig's anemia. Biol Reprod 45(5):719-26. [PubMed: 1756209]  [MGI Ref ID J:753]

Additional References

Cdk5rap2an related

Bannerman RM; Edwards JA; Pinkerton PH. 1973. Hereditary disorders of the red cell in animals. Prog Hematol 8:131-79. [PubMed: 4596202]  [MGI Ref ID J:5439]

Barker JE. 1995. Murine Hertwig's anemia: premature death after normal bone marrow transplantation is radiation dose-dependent. Blood 85(9):2627-31. [PubMed: 7727788]  [MGI Ref ID J:26056]

Bernstein SE. 1975. Bone marrow implantation therapy in Hertwig's anemic mice. Jackson Lab Ann Rep 46:87.  [MGI Ref ID J:13371]

Bernstein SE. 1969. Hereditary disorders of the rodent erythron. In: Genetics in Laboratory Animal Medicine. Natl Acad Sci Publ, Washington, DC.  [MGI Ref ID J:30699]

Harrison DE. 1976. Stem cell multiplication rate in W-anemic mice, and immune response of mice with Hertwig's anemia. Jackson Lab Ann Rep 47:59.  [MGI Ref ID J:13372]

Lizarraga SB; Margossian SP; Harris MH; Campagna DR; Han AP; Blevins S; Mudbhary R; Barker JE; Walsh CA; Fleming MD. 2010. Cdk5rap2 regulates centrosome function and chromosome segregation in neuronal progenitors. Development 137(11):1907-17. [PubMed: 20460369]  [MGI Ref ID J:160533]

Russell ES. 1970. Abnormalities of erythropoiesis associated with mutant genes in mice. In: Regulation of Hematopoiesis. Appleton-Century-Crofts, New York.  [MGI Ref ID J:27511]

Russell ES; Bernstein SE. 1966. Blood and Blood Formation. In: Biology of the Laboratory Mouse. McGraw Hill, New York.  [MGI Ref ID J:24829]

Russell ES; Meier H. 1966. Constitutional Diseases. In: Biology of the Laboratory Mouse. McGraw - Hill, New York.  [MGI Ref ID J:22618]

Russell ES; Whitten WK; Beamer WG; McFarland EC; Peters H. 1976. Germ cell defect in Hertwig's anemic mice Jackson Lab Ann Rep 47:89.  [MGI Ref ID J:15165]

Sassa S; Bernstein SE. 1978. Studies of erythrocyte protoporphyrin in anemic mutant mice: use of a modified hematofluorometer for the detection of heterozygotes for hemolytic disease. Exp Hematol 6(5):479-87. [PubMed: 658175]  [MGI Ref ID J:5985]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery of Strains Needing Progeny Testing
    At least two untested males and two untested females (two pairs) will be recovered (eight or more mice is typical). The total number of animals provided, their gender and genotype will vary. Untested animals typically are available to ship between 13 and 16 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation.
    Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation.

    Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 or 1-207-288-5845 (from any location). The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery of Strains Needing Progeny Testing
    At least two untested males and two untested females (two pairs) will be recovered (eight or more mice is typical). The total number of animals provided, their gender and genotype will vary. Untested animals typically are available to ship between 13 and 16 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation.
    Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation.

    Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 or 1-207-288-5845 (from any location). The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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