Description

Strain Information

Former Names C57BL/6-Tg(BCL2)25Wehi/J    (Changed: 24-JAN-06 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse   Donating Investigator Alan Harris,   The Walter & Eliza Hall Inst of Med Res

Appearance
black
Related Genotype: a/a

Description
Expression of the human BCL2 transgene restricted to the T cell lineage (no B-cell expression). Thymocytes, peripheral T-cells and activated T cells from these mice withstand prolonged culture in the absence of growth factors. Cells are resistant to killing by gamma-radiation, glucocorticoids, ionomycin, PMA and sodium azide but NOT complement, cytotoxic T cells or Fas ligand. Hemizygotes have a normal total T-cell count and thymic involution rate but show an enhanced response to immunization. This transgenic line displays no detectable autoimmunity. These mice serve as a robust source for the production of T-cell lines or hybridomas. Also known as 25Wehi or Emu-bcl-2-25.

Development
The TgN(BCL2)25Wehi transgenic strain was made in the laboratory of Dr. Alan Harris of the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia. The transgene construct consisted of the human BCL2 cDNA in association with the Emu immunoglobulin heavy chain enhancer and SV40 promoter. Original background was mix of C57BL/6JWehi and SJL/JWehi. Transgenic mice have been bred to C57BL/6J more than 20 generations.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of BCL2

002319	B6.Cg-Tg(BCL2)22Wehi/J
002321	B6.Cg-Tg(BCL2)36Wehi/J
002318	C.Cg-Tg(BCL2)22Wehi/J
002427	C3H/He-Tg(LCKprBCL2)36Sjk/J
002971	FVB-Tg(BCL2OVARY)1Ah/J

View Strains carrying other alleles of BCL2     (5 strains)

Strains carrying other alleles of SV40

002319	B6.Cg-Tg(BCL2)22Wehi/J
002321	B6.Cg-Tg(BCL2)36Wehi/J
002318	C.Cg-Tg(BCL2)22Wehi/J
003477	C57BL/6J-Tg(SV)419Bri/J
003476	C57BL/6J-Tg(SV)427Bri/J
003268	FVB-Tg(IRS1)1Mhep/J

View Strains carrying other alleles of SV40     (6 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tg(BCL2)25Wehi/0

        involves: C57BL/6JWehi * SJL/JWehi

• hematopoietic system phenotype
• increased T cell number (MGI Ref ID J:93111)
  • mutants have a 2-fold excess of T lymphocytes in peripheral lymphoid organs
• immune system phenotype
• abnormal T cell physiology (MGI Ref ID J:93111)
  • transgenic T cells from thymus or periphery show prolonged in vitro survival in culture compared to wild-type T lymphocytes
• increased T cell number (MGI Ref ID J:93111)
  • mutants have a 2-fold excess of T lymphocytes in peripheral lymphoid organs

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Apoptosis Research
Endogenous Regulators

Immunology and Inflammation Research
Intracellular Signaling Molecules

Research Tools
Cancer Research (production of T cells and hybridoma)
Immunology and Inflammation Research (production of T cell lines and hybridomas)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(BCL2)25Wehi
Allele Name		transgene insertion 25, Walter and Eliza Hall Institute of Medical Research
Allele Type		Transgenic (random, expressed)
Common Name(s)		25Wehi; Bcl2/Wehi25 Tg; Emu-2-25; Emu-bcl-2-25; hBcl-2;
Mutation Made By		Alan Harris,   The Walter & Eliza Hall Inst of Med Res
Strain of Origin		(C57BL/6JWehi x SJL/JWehi)F2
Expressed Gene		BCL2, B-cell CLL/lymphoma 2, human
Promoter		SV40, E mu enhancer, SV40
General Note		In transgenic mice on a C57BL/6 background, expression of the transgene is restricted to the T cell lineage (no B-cell expression). Thymocytes, peripheral T-cells and activated T cells from these mice withstand prolonged culture in the absence of growth factors. Cells are resistant to killing by gamma-radiation, glucocorticoids, ionomycin, PMA, and sodium azide, but not complement, cytotoxic T cells or Fas ligand. Hemizygotes have a normal total T-cell count and thymic involution rate but show an enhanced response to immunization. This transgenic line displays no detectable autoimmunity. These mice serve as a robust source for the production of T-cell lines or hybridomas.
Molecular Note		The transgene construct consists of the human BCL2 cDNA in association with the Emu immunoglobulin heavy chain enhancer and SV40 promoter. [MGI Ref ID J:93111]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(BCL2), STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Strasser A; Harris AW; Cory S. 1991. bcl-2 transgene inhibits T cell death and perturbs thymic self-censorship. Cell 67(5):889-99. [PubMed: 1959134]  [MGI Ref ID J:69572]

Additional References

Antov A; Yang L; Vig M; Baltimore D; Van Parijs L. 2003. Essential role for STAT5 signaling in CD25+CD4+ regulatory T cell homeostasis and the maintenance of self-tolerance. J Immunol 171(7):3435-41. [PubMed: 14500638]  [MGI Ref ID J:85631]

O'Reilly LA; Harris AW; Strasser A. 1997. bcl-2 transgene expression promotes survival and reduces proliferation of CD3-CD4-CD8- T cell progenitors. Int Immunol 9(9):1291-301. [PubMed: 9310832]  [MGI Ref ID J:93122]

Telfer JC; Hedblom EE; Anderson MK; Laurent MN; Rothenberg EV. 2004. Localization of the domains in Runx transcription factors required for the repression of CD4 in thymocytes. J Immunol 172(7):4359-70. [PubMed: 15034051]  [MGI Ref ID J:88730]

Tg(BCL2)25Wehi related

Akashi K; Kondo M; von Freeden-Jeffry U; Murray R; Weissman IL. 1997. Bcl-2 rescues T lymphopoiesis in interleukin-7 receptor-deficient mice. Cell 89(7):1033-41. [PubMed: 9215626]  [MGI Ref ID J:41241]

Antov A; Yang L; Vig M; Baltimore D; Van Parijs L. 2003. Essential role for STAT5 signaling in CD25+CD4+ regulatory T cell homeostasis and the maintenance of self-tolerance. J Immunol 171(7):3435-41. [PubMed: 14500638]  [MGI Ref ID J:85631]

Biju MP; Neumann AK; Bensinger SJ; Johnson RS; Turka LA; Haase VH. 2004. Vhlh gene deletion induces hif-1-mediated cell death in thymocytes. Mol Cell Biol 24(20):9038-47. [PubMed: 15456877]  [MGI Ref ID J:93322]

Chesi M; Robbiani DF; Sebag M; Chng WJ; Affer M; Tiedemann R; Valdez R; Palmer SE; Haas SS; Stewart AK; Fonseca R; Kremer R; Cattoretti G; Bergsagel PL. 2008. AID-dependent activation of a MYC transgene induces multiple myeloma in a conditional mouse model of post-germinal center malignancies. Cancer Cell 13(2):167-80. [PubMed: 18242516]  [MGI Ref ID J:131912]

Core N; Joly F; Boned A; Djabali M. 2004. Disruption of E2F signaling suppresses the INK4a-induced proliferative defect in M33-deficient mice. Oncogene 23(46):7660-8. [PubMed: 15377996]  [MGI Ref ID J:93837]

Cory S; Harris AW; Strasser A. 1994. Insights from transgenic mice regarding the role of bcl-2 in normal and neoplastic lymphoid cells. Philos Trans R Soc Lond B Biol Sci 345(1313):289-95. [PubMed: 7846127]  [MGI Ref ID J:21062]

Kondo M; Akashi K; Domen J; Sugamura K; Weissman IL. 1997. Bcl-2 rescues T lymphopoiesis, but not B or NK cell development, in common gamma chain-deficient mice. Immunity 7(1):155-62. [PubMed: 9252128]  [MGI Ref ID J:42537]

Kumar L; Feske S; Rao A; Geha RS. 2005. A 10-aa-long sequence in SLP-76 upstream of the Gads binding site is essential for T cell development and function. Proc Natl Acad Sci U S A 102(52):19063-8. [PubMed: 16354835]  [MGI Ref ID J:104696]

Masse GX; Corcuff E; Decaluwe H; Bommhardt U; Lantz O; Buer J; Di Santo JP. 2007. gamma(c) cytokines provide multiple homeostatic signals to naive CD4(+) T cells. Eur J Immunol 37(9):2606-16. [PubMed: 17683114]  [MGI Ref ID J:124346]

Masse GX; Corcuff E; Strick-Marchand H; Guy-Grand D; Tafuri-Bladt A; Albert ML; Lantz O; Di Santo JP. 2007. Gamma c cytokines condition the progressive differentiation of CD4+ T cells. Proc Natl Acad Sci U S A 104(39):15442-7. [PubMed: 17855567]  [MGI Ref ID J:125207]

Newton K; Strasser A. 2000. Ionizing radiation and chemotherapeutic drugs induce apoptosis in lymphocytes in the absence of Fas or FADD/MORT1 signaling. Implications for cancer therapy. J Exp Med 191(1):195-200. [PubMed: 10620618]  [MGI Ref ID J:59250]

O'Reilly LA; Harris AW; Strasser A. 1997. bcl-2 transgene expression promotes survival and reduces proliferation of CD3-CD4-CD8- T cell progenitors. Int Immunol 9(9):1291-301. [PubMed: 9310832]  [MGI Ref ID J:93122]

Ohteki T; Maki C; Koyasu S. 2001. Overexpression of Bcl-2 differentially restores development of thymus-derived CD4-8+ T cells and intestinal intraepithelial T cells in IFN-regulatory factor-1-deficient mice. J Immunol 166(11):6509-13. [PubMed: 11359801]  [MGI Ref ID J:69487]

Pearson R; Weston K. 2000. c-Myb regulates the proliferation of immature thymocytes following beta-selection. EMBO J 19(22):6112-20. [PubMed: 11080157]  [MGI Ref ID J:115111]

Petschner F; Zimmerman C; Strasser A; Grillot D; Nunez G; Pircher H. 1998. Constitutive expression of Bcl-xL or Bcl-2 prevents peptide antigen-induced T cell deletion but does not influence T cell homeostasis after a viral infection. Eur J Immunol 28(2):560-9. [PubMed: 9521066]  [MGI Ref ID J:111125]

Priceputu E; Rodrigue I; Chrobak P; Poudrier J; Mak TW; Hanna Z; Hu C; Kay DG; Jolicoeur P. 2005. The Nef-mediated AIDS-like disease of CD4C/human immunodeficiency virus transgenic mice is associated with increased Fas/FasL expression on T cells and T-cell death but is not prevented in Fas-, FasL-, tumor necrosis factor receptor 1-, or interleukin-1beta-converting enzyme-deficient or Bcl2-expressing transgenic mice. J Virol 79(10):6377-91. [PubMed: 15858021]  [MGI Ref ID J:98353]

Ranson T; Vosshenrich CA; Corcuff E; Richard O; Muller W; Di Santo JP. 2003. IL-15 is an essential mediator of peripheral NK-cell homeostasis. Blood 101(12):4887-93. [PubMed: 12586624]  [MGI Ref ID J:132257]

Redmond WL; Wei CH; Kreuwel HT; Sherman LA. 2008. The apoptotic pathway contributing to the deletion of naive CD8 T cells during the induction of peripheral tolerance to a cross-presented self-antigen. J Immunol 180(8):5275-82. [PubMed: 18390708]  [MGI Ref ID J:134242]

Rodewald HR; Waskow C; Haller C. 2001. Essential requirement for c-kit and common gamma chain in thymocyte development cannot be overruled by enforced expression of Bcl-2. J Exp Med 193(12):1431-7. [PubMed: 11413198]  [MGI Ref ID J:70009]

Rodriguez-Galan MC; Bream JH; Farr A; Young HA. 2005. Synergistic effect of IL-2, IL-12, and IL-18 on thymocyte apoptosis and Th1/Th2 cytokine expression. J Immunol 174(5):2796-804. [PubMed: 15728489]  [MGI Ref ID J:97710]

Rolink A; Melchers F; Andersson J. 1996. The SCID but not the RAG-2 gene product is required for S mu-S epsilon heavy chain class switching. Immunity 5(4):319-30. [PubMed: 8885865]  [MGI Ref ID J:36026]

Strasser A; Harris AW; Huang DC; Krammer PH; Cory S. 1995. Bcl-2 and Fas/APO-1 regulate distinct pathways to lymphocyte apoptosis. EMBO J 14(24):6136-47. [PubMed: 8557033]  [MGI Ref ID J:31382]

Strasser A; Harris AW; Vaux DL; Webb E; Bath ML; Adams JM; Cory S. 1990. Abnormalities of the immune system induced by dysregulated bcl-2 expression in transgenic mice. Curr Top Microbiol Immunol 166:175-81. [PubMed: 2073796]  [MGI Ref ID J:93111]

Strasser A; Harris AW; von Boehmer H; Cory S. 1994. Positive and negative selection of T cells in T-cell receptor transgenic mice expressing a bcl-2 transgene. Proc Natl Acad Sci U S A 91(4):1376-80. [PubMed: 8108419]  [MGI Ref ID J:16947]

Uehara S; Hayes SM; Li L; El-Khoury D; Canelles M; Fowlkes BJ; Love PE. 2006. Premature expression of chemokine receptor CCR9 impairs T cell development. J Immunol 176(1):75-84. [PubMed: 16365398]  [MGI Ref ID J:126252]

Van Dyken SJ; Green RS; Marth JD. 2007. Structural and mechanistic features of protein O glycosylation linked to CD8+ T-cell apoptosis. Mol Cell Biol 27(3):1096-111. [PubMed: 17101770]  [MGI Ref ID J:118162]

Van Parijs L; Biuckians A; Abbas AK. 1998. Functional roles of Fas and Bcl-2-regulated apoptosis of T lymphocytes. J Immunol 160(5):2065-71. [PubMed: 9498742]  [MGI Ref ID J:112062]

Yajima T; Yoshihara K; Nakazato K; Kumabe S; Koyasu S; Sad S; Shen H; Kuwano H; Yoshikai Y. 2006. IL-15 regulates CD8+ T cell contraction during primary infection. J Immunol 176(1):507-15. [PubMed: 16365444]  [MGI Ref ID J:126257]

Zhang X; Fujii H; Kishimoto H; LeRoy E; Surh CD; Sprent J. 2002. Aging leads to disturbed homeostasis of memory phenotype CD8(+) cells. J Exp Med 195(3):283-93. [PubMed: 11828003]  [MGI Ref ID J:124828]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	This strain is maintained by mating hemizygous mice with wildtype siblings. Expected coat color from breeding:Black
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.