Strain Name:

B6.Cg-Tg(BCL2)25Wehi/J

Stock Number:

002320

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C57BL/6-Tg(BCL2)25Wehi/J    (Changed: 24-JAN-06 )
Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Alan Harris,   The Walter & Eliza Hall Inst of Med Res

Appearance
black
Related Genotype: a/a

Description
Expression of the human BCL2 transgene restricted to the T cell lineage (no B-cell expression). Thymocytes, peripheral T-cells and activated T cells from these mice withstand prolonged culture in the absence of growth factors. Cells are resistant to killing by gamma-radiation, glucocorticoids, ionomycin, PMA and sodium azide but NOT complement, cytotoxic T cells or Fas ligand. Hemizygotes have a normal total T-cell count and thymic involution rate but show an enhanced response to immunization. This transgenic line displays no detectable autoimmunity. These mice serve as a robust source for the production of T-cell lines or hybridomas. Also known as 25Wehi or Emu-bcl-2-25.

Development
The TgN(BCL2)25Wehi transgenic strain was made in the laboratory of Dr. Alan Harris of the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia. The transgene construct consisted of the human BCL2 cDNA in association with the Emu immunoglobulin heavy chain enhancer and SV40 promoter. Original background was mix of C57BL/6JWehi and SJL/JWehi. Transgenic mice have been bred to C57BL/6J more than 20 generations.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of BCL2
002319   B6.Cg-Tg(BCL2)22Wehi/J
002321   B6.Cg-Tg(BCL2)36Wehi/J
002318   C.Cg-Tg(BCL2)22Wehi/J
002427   C3H/He-Tg(LCKprBCL2)36Sjk/J
002971   FVB-Tg(BCL2OVARY)1Ah/J
View Strains carrying other alleles of BCL2     (5 strains)

View Strains carrying other alleles of SV40     (13 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested.
B-Cell Cll/Lymphoma 2; BCL2   (BCL2)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Tg(BCL2)25Wehi/0

        involves: C57BL/6JWehi * SJL/JWehi
  • hematopoietic system phenotype
  • abnormal B cell differentiation
    • benign polyclonal B cell and plasma cell expansion without spikes in monoclonal antibodies (M-spikes)   (MGI Ref ID J:131912)
    • abnormal plasma cell differentiation
      • benign polyclonal B cell and plasma cell expansion without spikes in monoclonal antibodies (M-spikes)   (MGI Ref ID J:131912)
  • abnormal T cell physiology
    • transgenic T cells from thymus or periphery show prolonged in vitro survival in culture compared to wild-type T lymphocytes   (MGI Ref ID J:93111)
  • increased T cell number
    • mutants have a 2-fold excess of T lymphocytes in peripheral lymphoid organs   (MGI Ref ID J:93111)
  • immune system phenotype
  • abnormal B cell differentiation
    • benign polyclonal B cell and plasma cell expansion without spikes in monoclonal antibodies (M-spikes)   (MGI Ref ID J:131912)
    • abnormal plasma cell differentiation
      • benign polyclonal B cell and plasma cell expansion without spikes in monoclonal antibodies (M-spikes)   (MGI Ref ID J:131912)
  • abnormal T cell physiology
    • transgenic T cells from thymus or periphery show prolonged in vitro survival in culture compared to wild-type T lymphocytes   (MGI Ref ID J:93111)
  • increased T cell number
    • mutants have a 2-fold excess of T lymphocytes in peripheral lymphoid organs   (MGI Ref ID J:93111)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Apoptosis Research
Endogenous Regulators

Immunology, Inflammation and Autoimmunity Research
Intracellular Signaling Molecules

Research Tools
Cancer Research
      production of T cells and hybridoma
Immunology, Inflammation and Autoimmunity Research
      production of T cell lines and hybridomas

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(BCL2)25Wehi
Allele Name transgene insertion 25, Walter and Eliza Hall Institute of Medical Research
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) 25Wehi; Bcl2/Wehi25 Tg; Emu-2-25; Emu-bcl-2-25; H2k-Bcl2-tg; hBcl-2;
Mutation Made ByDr. Alan Harris,   The Walter & Eliza Hall Inst of Med Res
Strain of Origin(C57BL/6JWehi x SJL/JWehi)F2
Expressed Gene BCL2, B-cell CLL/lymphoma 2, human
Promoter SV40, E mu enhancer, SV40
General Note

In transgenic mice on a C57BL/6 background, expression of the transgene is restricted to the T cell lineage (no B-cell expression).

Thymocytes, peripheral T-cells and activated T cells from these mice withstand prolonged culture in the absence of growth factors. Cells are resistant to killing by gamma-radiation, glucocorticoids, ionomycin, PMA, and sodium azide, but not complement, cytotoxic T cells or Fas ligand. Hemizygotes have a normal total T-cell count and thymic involution rate but show an enhanced response to immunization. This transgenic line displays no detectable autoimmunity. These mice serve as a robust source for the production of T-cell lines or hybridomas.

Molecular Note The transgene construct consists of the human BCL2 cDNA in association with the Emu immunoglobulin heavy chain enhancer and SV40 promoter. [MGI Ref ID J:93111]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(BCL2),

MELT



Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Strasser A; Harris AW; Cory S. 1991. bcl-2 transgene inhibits T cell death and perturbs thymic self-censorship. Cell 67(5):889-99. [PubMed: 1959134]  [MGI Ref ID J:69572]

Additional References

Antov A; Yang L; Vig M; Baltimore D; Van Parijs L. 2003. Essential role for STAT5 signaling in CD25+CD4+ regulatory T cell homeostasis and the maintenance of self-tolerance. J Immunol 171(7):3435-41. [PubMed: 14500638]  [MGI Ref ID J:85631]

O'Reilly LA; Harris AW; Strasser A. 1997. bcl-2 transgene expression promotes survival and reduces proliferation of CD3-CD4-CD8- T cell progenitors. Int Immunol 9(9):1291-301. [PubMed: 9310832]  [MGI Ref ID J:93122]

Telfer JC; Hedblom EE; Anderson MK; Laurent MN; Rothenberg EV. 2004. Localization of the domains in Runx transcription factors required for the repression of CD4 in thymocytes. J Immunol 172(7):4359-70. [PubMed: 15034051]  [MGI Ref ID J:88730]

Tg(BCL2)25Wehi related

Akashi K; Kondo M; von Freeden-Jeffry U; Murray R; Weissman IL. 1997. Bcl-2 rescues T lymphopoiesis in interleukin-7 receptor-deficient mice. Cell 89(7):1033-41. [PubMed: 9215626]  [MGI Ref ID J:41241]

Antov A; Yang L; Vig M; Baltimore D; Van Parijs L. 2003. Essential role for STAT5 signaling in CD25+CD4+ regulatory T cell homeostasis and the maintenance of self-tolerance. J Immunol 171(7):3435-41. [PubMed: 14500638]  [MGI Ref ID J:85631]

Balkhi MY; Willette-Brown J; Zhu F; Chen Z; Liu S; Guttridge DC; Karin M; Hu Y. 2012. IKKalpha-mediated signaling circuitry regulates early B lymphopoiesis during hematopoiesis. Blood 119(23):5467-77. [PubMed: 22544702]  [MGI Ref ID J:188639]

Biju MP; Neumann AK; Bensinger SJ; Johnson RS; Turka LA; Haase VH. 2004. Vhlh gene deletion induces hif-1-mediated cell death in thymocytes. Mol Cell Biol 24(20):9038-47. [PubMed: 15456877]  [MGI Ref ID J:93322]

Chesi M; Robbiani DF; Sebag M; Chng WJ; Affer M; Tiedemann R; Valdez R; Palmer SE; Haas SS; Stewart AK; Fonseca R; Kremer R; Cattoretti G; Bergsagel PL. 2008. AID-dependent activation of a MYC transgene induces multiple myeloma in a conditional mouse model of post-germinal center malignancies. Cancer Cell 13(2):167-80. [PubMed: 18242516]  [MGI Ref ID J:131912]

Core N; Joly F; Boned A; Djabali M. 2004. Disruption of E2F signaling suppresses the INK4a-induced proliferative defect in M33-deficient mice. Oncogene 23(46):7660-8. [PubMed: 15377996]  [MGI Ref ID J:93837]

Cory S; Harris AW; Strasser A. 1994. Insights from transgenic mice regarding the role of bcl-2 in normal and neoplastic lymphoid cells. Philos Trans R Soc Lond B Biol Sci 345(1313):289-95. [PubMed: 7846127]  [MGI Ref ID J:21062]

Del Real MM; Rothenberg EV. 2013. Architecture of a lymphomyeloid developmental switch controlled by PU.1, Notch and Gata3. Development 140(6):1207-19. [PubMed: 23444353]  [MGI Ref ID J:194844]

Franco CB; Scripture-Adams DD; Proekt I; Taghon T; Weiss AH; Yui MA; Adams SL; Diamond RA; Rothenberg EV. 2006. Notch/Delta signaling constrains reengineering of pro-T cells by PU.1. Proc Natl Acad Sci U S A 103(32):11993-8. [PubMed: 16880393]  [MGI Ref ID J:111829]

Hale JS; Nelson LT; Simmons KB; Fink PJ. 2011. Bcl-2-interacting mediator of cell death influences autoantigen-driven deletion and TCR revision. J Immunol 186(2):799-806. [PubMed: 21148799]  [MGI Ref ID J:168782]

Khandanpour C; Kosan C; Gaudreau MC; Duhrsen U; Hebert J; Zeng H; Moroy T. 2011. Growth factor independence 1 protects hematopoietic stem cells against apoptosis but also prevents the development of a myeloproliferative-like disease. Stem Cells 29(2):376-85. [PubMed: 21732494]  [MGI Ref ID J:190207]

Khandanpour C; Phelan JD; Vassen L; Schutte J; Chen R; Horman SR; Gaudreau MC; Krongold J; Zhu J; Paul WE; Duhrsen U; Gottgens B; Grimes HL; Moroy T. 2013. Growth factor independence 1 antagonizes a p53-induced DNA damage response pathway in lymphoblastic leukemia. Cancer Cell 23(2):200-14. [PubMed: 23410974]  [MGI Ref ID J:194309]

Kondo M; Akashi K; Domen J; Sugamura K; Weissman IL. 1997. Bcl-2 rescues T lymphopoiesis, but not B or NK cell development, in common gamma chain-deficient mice. Immunity 7(1):155-62. [PubMed: 9252128]  [MGI Ref ID J:42537]

Kumar L; Feske S; Rao A; Geha RS. 2005. A 10-aa-long sequence in SLP-76 upstream of the Gads binding site is essential for T cell development and function. Proc Natl Acad Sci U S A 102(52):19063-8. [PubMed: 16354835]  [MGI Ref ID J:104696]

Lalanne AI; Moraga I; Hao Y; Pereira JP; Alves NL; Huntington ND; Freitas AA; Cumano A; Vieira P. 2010. CpG inhibits pro-B cell expansion through a cathepsin B-dependent mechanism. J Immunol 184(10):5678-85. [PubMed: 20400700]  [MGI Ref ID J:160995]

Masse GX; Corcuff E; Decaluwe H; Bommhardt U; Lantz O; Buer J; Di Santo JP. 2007. gamma(c) cytokines provide multiple homeostatic signals to naive CD4(+) T cells. Eur J Immunol 37(9):2606-16. [PubMed: 17683114]  [MGI Ref ID J:124346]

Masse GX; Corcuff E; Strick-Marchand H; Guy-Grand D; Tafuri-Bladt A; Albert ML; Lantz O; Di Santo JP. 2007. Gamma c cytokines condition the progressive differentiation of CD4+ T cells. Proc Natl Acad Sci U S A 104(39):15442-7. [PubMed: 17855567]  [MGI Ref ID J:125207]

Nakazato K; Yamada H; Yajima T; Kagimoto Y; Kuwano H; Yoshikai Y. 2007. Enforced expression of Bcl-2 partially restores cell numbers but not functions of TCRgammadelta intestinal intraepithelial T lymphocytes in IL-15-deficient mice. J Immunol 178(2):757-64. [PubMed: 17202336]  [MGI Ref ID J:142633]

Newton K; Strasser A. 2000. Ionizing radiation and chemotherapeutic drugs induce apoptosis in lymphocytes in the absence of Fas or FADD/MORT1 signaling. Implications for cancer therapy. J Exp Med 191(1):195-200. [PubMed: 10620618]  [MGI Ref ID J:59250]

O'Reilly LA; Harris AW; Strasser A. 1997. bcl-2 transgene expression promotes survival and reduces proliferation of CD3-CD4-CD8- T cell progenitors. Int Immunol 9(9):1291-301. [PubMed: 9310832]  [MGI Ref ID J:93122]

Ohteki T; Maki C; Koyasu S. 2001. Overexpression of Bcl-2 differentially restores development of thymus-derived CD4-8+ T cells and intestinal intraepithelial T cells in IFN-regulatory factor-1-deficient mice. J Immunol 166(11):6509-13. [PubMed: 11359801]  [MGI Ref ID J:69487]

Pearson R; Weston K. 2000. c-Myb regulates the proliferation of immature thymocytes following beta-selection. EMBO J 19(22):6112-20. [PubMed: 11080157]  [MGI Ref ID J:115111]

Petschner F; Zimmerman C; Strasser A; Grillot D; Nunez G; Pircher H. 1998. Constitutive expression of Bcl-xL or Bcl-2 prevents peptide antigen-induced T cell deletion but does not influence T cell homeostasis after a viral infection. Eur J Immunol 28(2):560-9. [PubMed: 9521066]  [MGI Ref ID J:111125]

Priceputu E; Rodrigue I; Chrobak P; Poudrier J; Mak TW; Hanna Z; Hu C; Kay DG; Jolicoeur P. 2005. The Nef-mediated AIDS-like disease of CD4C/human immunodeficiency virus transgenic mice is associated with increased Fas/FasL expression on T cells and T-cell death but is not prevented in Fas-, FasL-, tumor necrosis factor receptor 1-, or interleukin-1beta-converting enzyme-deficient or Bcl2-expressing transgenic mice. J Virol 79(10):6377-91. [PubMed: 15858021]  [MGI Ref ID J:98353]

Ranson T; Vosshenrich CA; Corcuff E; Richard O; Muller W; Di Santo JP. 2003. IL-15 is an essential mediator of peripheral NK-cell homeostasis. Blood 101(12):4887-93. [PubMed: 12586624]  [MGI Ref ID J:132257]

Redmond WL; Wei CH; Kreuwel HT; Sherman LA. 2008. The apoptotic pathway contributing to the deletion of naive CD8 T cells during the induction of peripheral tolerance to a cross-presented self-antigen. J Immunol 180(8):5275-82. [PubMed: 18390708]  [MGI Ref ID J:134242]

Rodewald HR; Waskow C; Haller C. 2001. Essential requirement for c-kit and common gamma chain in thymocyte development cannot be overruled by enforced expression of Bcl-2. J Exp Med 193(12):1431-7. [PubMed: 11413198]  [MGI Ref ID J:70009]

Rodriguez-Galan MC; Bream JH; Farr A; Young HA. 2005. Synergistic effect of IL-2, IL-12, and IL-18 on thymocyte apoptosis and Th1/Th2 cytokine expression. J Immunol 174(5):2796-804. [PubMed: 15728489]  [MGI Ref ID J:97710]

Rolink A; Melchers F; Andersson J. 1996. The SCID but not the RAG-2 gene product is required for S mu-S epsilon heavy chain class switching. Immunity 5(4):319-30. [PubMed: 8885865]  [MGI Ref ID J:36026]

Strasser A; Harris AW; Huang DC; Krammer PH; Cory S. 1995. Bcl-2 and Fas/APO-1 regulate distinct pathways to lymphocyte apoptosis. EMBO J 14(24):6136-47. [PubMed: 8557033]  [MGI Ref ID J:31382]

Strasser A; Harris AW; Vaux DL; Webb E; Bath ML; Adams JM; Cory S. 1990. Abnormalities of the immune system induced by dysregulated bcl-2 expression in transgenic mice. Curr Top Microbiol Immunol 166:175-81. [PubMed: 2073796]  [MGI Ref ID J:93111]

Strasser A; Harris AW; von Boehmer H; Cory S. 1994. Positive and negative selection of T cells in T-cell receptor transgenic mice expressing a bcl-2 transgene. Proc Natl Acad Sci U S A 91(4):1376-80. [PubMed: 8108419]  [MGI Ref ID J:16947]

Taghon T; Yui MA; Rothenberg EV. 2007. Mast cell lineage diversion of T lineage precursors by the essential T cell transcription factor GATA-3. Nat Immunol 8(8):845-855. [PubMed: 17603486]  [MGI Ref ID J:123414]

Tani-Ichi S; Satake M; Ikuta K. 2011. The pre-TCR signal induces transcriptional silencing of the TCRgamma locus by reducing the recruitment of STAT5 and Runx to transcriptional enhancers. Int Immunol 23(9):553-63. [PubMed: 21750145]  [MGI Ref ID J:176159]

Uehara S; Hayes SM; Li L; El-Khoury D; Canelles M; Fowlkes BJ; Love PE. 2006. Premature expression of chemokine receptor CCR9 impairs T cell development. J Immunol 176(1):75-84. [PubMed: 16365398]  [MGI Ref ID J:126252]

Van Dyken SJ; Green RS; Marth JD. 2007. Structural and mechanistic features of protein O glycosylation linked to CD8+ T-cell apoptosis. Mol Cell Biol 27(3):1096-111. [PubMed: 17101770]  [MGI Ref ID J:118162]

Van Laethem F; Sarafova SD; Park JH; Tai X; Pobezinsky L; Guinter TI; Adoro S; Adams A; Sharrow SO; Feigenbaum L; Singer A. 2007. Deletion of CD4 and CD8 Coreceptors Permits Generation of alphabetaT Cells that Recognize Antigens Independently of the MHC. Immunity 27(5):735-50. [PubMed: 18023370]  [MGI Ref ID J:127623]

Van Parijs L; Biuckians A; Abbas AK. 1998. Functional roles of Fas and Bcl-2-regulated apoptosis of T lymphocytes. J Immunol 160(5):2065-71. [PubMed: 9498742]  [MGI Ref ID J:112062]

Wong WF; Kohu K; Nakamura A; Ebina M; Kikuchi T; Tazawa R; Tanaka K; Kon S; Funaki T; Sugahara-Tobinai A; Looi CY; Endo S; Funayama R; Kurokawa M; Habu S; Ishii N; Fukumoto M; Nakata K; Takai T; Satake M. 2012. Runx1 deficiency in CD4+ T cells causes fatal autoimmune inflammatory lung disease due to spontaneous hyperactivation of cells. J Immunol 188(11):5408-20. [PubMed: 22551552]  [MGI Ref ID J:188720]

Yajima T; Yoshihara K; Nakazato K; Kumabe S; Koyasu S; Sad S; Shen H; Kuwano H; Yoshikai Y. 2006. IL-15 regulates CD8+ T cell contraction during primary infection. J Immunol 176(1):507-15. [PubMed: 16365444]  [MGI Ref ID J:126257]

Yuan J; Crittenden RB; Bender TP. 2010. c-Myb promotes the survival of CD4+CD8+ double-positive thymocytes through upregulation of Bcl-xL. J Immunol 184(6):2793-804. [PubMed: 20142358]  [MGI Ref ID J:160124]

Yui MA; Feng N; Rothenberg EV. 2010. Fine-scale staging of T cell lineage commitment in adult mouse thymus. J Immunol 185(1):284-93. [PubMed: 20543111]  [MGI Ref ID J:161594]

Zhang X; Fujii H; Kishimoto H; LeRoy E; Surh CD; Sprent J. 2002. Aging leads to disturbed homeostasis of memory phenotype CD8(+) cells. J Exp Med 195(3):283-93. [PubMed: 11828003]  [MGI Ref ID J:124828]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThis strain is maintained by mating hemizygous mice with wildtype siblings. Expected coat color from breeding:Black

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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