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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6C3Fe-a/a-nma (Changed: 15-DEC-04 ) Type Mutant Stock; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N23 Appearance
black
Related Genotype: a/aDescription
nma arose spontaneously in 1985 on the CBA/J inbred strain at The Jackson Laboratory. From the age of 12 days, mice homozygous for nma are smaller than their normal littermates, weighing 50% less. They exhibit an abnormal gait, in which the hind legs extend past the front legs, and hind limb weakness that causes the mice to fall to the side. When sitting, nma/nma mice overextend their rear legs and prop themselves up in the shavings or against the cage wall. When picked up by the tail, homozygotes extend their rear legs briefly, then clasp their hind feet. They cannot orient themselves in water and spiral to the bottom of the tank. nma/nma mice survive only a few days past weaning, even with food and water made readily available. Heterozygotes appear normal behaviorally, histologically and biochemically.Examination of serial sections through the entire brain revealed no abnormalities. The spinal cord motor neurons, spinal roots, ganglia and nerves were normally organized and myelinated. There was no muscular dystrophy or neurogenic atrophy of the skeletal muscle. Somatic organs appeared normal. Bone and muscle from the fore- and hindlimbs "showed no consistent abnormalities" and appeared normally innervated. X-ray analysis revealed normal hip joint structure, excluding hip abnormality as the reason for the hind limb overextension. ABR threshold analysis indicated that mice homozygous for nma have normal hearing at 18-21 days of age, and histological analysis of inner ears revealed no anomalies. Homozygotes exhibited significantly lower serum glucose and alanine aminotransferase (ALT) levels than normal littermates; blood urea nitrogen (BUN) did not differ. Heterozygote values were intermediate between those of the F1 parental strains, indicating that heterozygosity for nma has no effect on these parameters (Ward-Bailey et al. 2000).
Development
The mutation neuromuscular ataxia (nma) arose spontaneously in strain CBA/J at generation F195 at The Jackson Laboratory in 1985. A carrier mouse was outcrossed to a B6C3Fe-a/a F1 and the F1 pairs were mated. It was continually backcrossed to the hybrid B6C3Fe-a/a by the cross-intercross method. It was cryopreserved in 1995 by mating heterozygotes at generation N22+.
Related Strains
Strains carrying a allele
View Strains carrying a (104 strains)
Phenotype
Phenotype Information
assigned by genotype
nma/nma
B6C3Fe a/a-nma/J
- life span-post-weaning/aging
- premature death (MGI Ref ID J:60439)
- growth/size phenotype
- decreased body size (MGI Ref ID J:60439)
- small size is noticeable by 12 days of age
- decreased body weight (MGI Ref ID J:60439)
- mutants weigh 50% less than control littermates
- behavior/neurological phenotype
- abnormal gait (MGI Ref ID J:60439)
- dysmetria (MGI Ref ID J:60439)
- rear legs are overextended when sitting and extend beyond the front legs when walking
- impaired balance (MGI Ref ID J:60439)
- mutants have problems maintaining their balance and appear to prop themselves up in the shavings or against the cage wall when sitting
- when placed in water, mutants cannot orient body position and go to the bottom of the container in a rolling spiral
- limb grasping (MGI Ref ID J:60439)
- when picked up the tail, the rear legs are held in an extended position for a brief period and then the legs clasp
- weakness (MGI Ref ID J:60439)
- mutants are frail and exhibit a general weakness
- weakness in the hindlimbs that causes mutant to fall to one side and then the other
- homeostasis/metabolism phenotype
- decreased circulating glucose level (MGI Ref ID J:60439)
- significantly reduced blood glucose
- nervous system phenotype
- *normal* nervous system phenotype (MGI Ref ID J:60439)
- although the behavioral abnormalities appear similar to mice that have cerebellum abnormalities, brain sections reveal no abnormalities, especially in the cerebellum
- muscle phenotype
- *normal* muscle phenotype (MGI Ref ID J:60439)
- skeletal muscle shows no muscular dystrophy or neurogenic atrophy
- hearing/vestibular/ear phenotype
- *normal* hearing/vestibular/ear phenotype (MGI Ref ID J:60439)
- normal hearing and ears
This mouse can be used to support research in many areas including:nma related
Neurobiology Research
Ataxia (Movement) Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | a | ||
|---|---|---|---|
| Allele Name | nonagouti | ||
| Allele Type | Spontaneous | ||
| Allele Symbol | nma | ||
| Allele Name | neuromuscular ataxia | ||
| Allele Type | Spontaneous | ||
| Strain of Origin | CBA/J | ||
| Gene Symbol and Name | nma, neuromuscular ataxia | ||
| Chromosome | 12 | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Additional References
nma relatedWard-Bailey PF; Wood B; Johnson KR; Bronson RT; Donahue LR; Davisson MT. 2000. Neuromuscular ataxia: a new spontaneous mutation in the mouse Mamm Genome 11(10):820-3. [PubMed: 11003693] [MGI Ref ID J:60439]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Payment Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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