Description

Strain Information

Former Names NOD.NOR-(D2Mit490-D2Mit144)/LtJ    (Changed: 25-APR-06 ) NOD.NOR-(D2Mit490-D2Mit144)/Lt    (Changed: 15-SEP-05 ) NOD.NOR-(D2Mit490-D2Mit144)    (Changed: 21-FEB-05 ) Type Congenic; Mutant Strain; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Background Strain NOD/ShiLt Donor Strain NOR/Lt H2 Haplotype g7   Donating Investigator Edward Leiter,   The Jackson Laboratory

Appearance
albino, pink eyed
Related Genotype: A/A Tyr^c/Tyr^c

Description
This NOD/Lt congenic strain is carrying alleles derived from strain NOR/Lt at the interleukin 1b (Il1b^NOR) and the insulin dependent diabetes susceptibility 13 (Idd13^r) loci. Incidence of diabetes among NOD.NOR-D2Mit490-Ada (Idd13^r, Il1b^NOR) females was 16% and 26% at 30 weeks and 52 weeks of age, respectively, compared with 100% diabetes incidence by 30 weeks in NOD/Lt females; no diabetes was observed in congenic males. Histologic examination of the pancreases of year-old non-diabetic congenic mice revealed extensive perivascular/periductal leukocytic infiltration; infiltrates associated with islets "were primarily peri-insular, with granulated beta cell mass largely preserved in >80% of the islets. This histopathology is similar to that observed in year-old NOR/Lt mice of both sexes. "Thus, Idd13^rdoes not mediate IDDM resistance in NOR mice by preventing lymphocyte infiltration of the pancreas, but by inhibiting "activation of autoimmune effector functions." This strain is useful for dissecting the role of non-MHC susceptibility genes in autoimmune diabetes.

Development
Mice of a congenic strain (at N12) carrying a defined segment of Chr 2 that included the IDDM resistant allele of NOR/Lt at the insulin dependent diabetes susceptibility locus Idd13 were further backcrossed to NOD/Lt. At backcross generation N21, the truncated NOR-derived segment of Chr 2 was fixed to homozygosity in each of three lines by sib mating. NOD.NOR-D2Mit490-Ada (Idd13^r , Il1b^NOR) carries a 31.5 cM interval of Chr 2 from NOR/Lt extending from D2Mit490 through Ada that "contains all markers previously found to be linked to Idd13 -mediated IDDM resistance in NOR mice." This region of Chr 2 in NOR was originally derived from the C57BL/6 contribution to strain C57BLKS/J; thus, this congenic strain carries the B6 alleles for the microsatellite markers and the functional genes (e.g., Zfp106^a/H3a^a, B2m^b , and presumably Il1b) in this region. (Serreze et al. refer to this strain as NOD.D2Mit490-Mit144^NOR .)

Control Information

	Control
	001976 NOD/ShiLtJ
		NOD/LtJ (001976) mice may be used as diabetes-susceptible controls.
Considerations for Choosing Controls

Related Strains

Strains carrying   Idd13^NOR/Lt allele

003051	NOD.NOR-(Il1a-Pcna)/LtJ
003050	NOD.NOR-(Zfp106-Il1a)/LtJ

View Strains carrying   Idd13^NOR/Lt     (2 strains)

Additional Web Information

Congenic Nomenclature
JAX^® NOTES, Spring 1999; 477. Control Strains for NOD/LtJ Mice in Diabetes Research.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Idd13^NOR/Lt/Idd13^NOR/Lt

        involves: NOD/Lt * NOR/Lt

• homeostasis/metabolism phenotype
• abnormal glucose homeostasis (MGI Ref ID J:20685)
  • resistance to type 1 diabetes

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)

Genes & Alleles

Gene & Allele Information

Gene Symbol and Name		D2Mit490, DNA Segment, Chr 2 Massachusetts Institute of Technology 490
Chromosome		2
Gene Symbol and Name		Ada, adenosine deaminase
Chromosome		2
Gene Common Name(s)		MGC108610;
Allele Symbol		Idd13NOR/Lt
Allele Name		NOR/Lt
Allele Type		QTL
Strain of Origin		NOR/Lt
Gene Symbol and Name		Idd13, insulin dependent diabetes susceptibility 13
Chromosome		2
Gene Symbol and Name		Il1b, interleukin 1 beta
Chromosome		2
Gene Common Name(s)		IL-1; IL-1B; IL-1beta; IL1-BETA; IL1F2; Il-1b;

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Serreze DV; Prochazka M; Reifsnyder PC; Bridgett MM; Leiter EH. 1994. Use of recombinant congenic and congenic strains of NOD mice to identify a new insulin-dependent diabetes resistance gene. J Exp Med 180(4):1553-8. [PubMed: 7931087]  [MGI Ref ID J:20685]

Additional References

Serreze DV; Bridgett M; Chapman HD; Chen E; Richard SD; Leiter EH. 1998. Subcongenic analysis of the Idd13 locus in NOD/Lt mice: evidence for several susceptibility genes including a possible diabetogenic role for beta 2-microglobulin. J Immunol 160(3):1472-8. [PubMed: 9570569]  [MGI Ref ID J:44130]

Idd13^NOR/Lt related

Serreze DV; Bridgett M; Chapman HD; Chen E; Richard SD; Leiter EH. 1998. Subcongenic analysis of the Idd13 locus in NOD/Lt mice: evidence for several susceptibility genes including a possible diabetogenic role for beta 2-microglobulin. J Immunol 160(3):1472-8. [PubMed: 9570569]  [MGI Ref ID J:44130]

Silveira PA; Chapman HD; Stolp J; Johnson E; Cox SL; Hunter K; Wicker LS; Serreze DV. 2006. Genes within the Idd5 and Idd9/11 Diabetes Susceptibility Loci Affect the Pathogenic Activity of B Cells in Nonobese Diabetic Mice. J Immunol 177(10):7033-41. [PubMed: 17082619]  [MGI Ref ID J:114754]

Takenaka K; Prasolava TK; Wang JC; Mortin-Toth SM; Khalouei S; Gan OI; Dick JE; Danska JS. 2007. Polymorphism in Sirpa modulates engraftment of human hematopoietic stem cells. Nat Immunol 8(12):1313-23. [PubMed: 17982459]  [MGI Ref ID J:127760]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Type 1 Diabetes Repository collection.

Control Information

	Control
	001976 NOD/ShiLtJ
		NOD/LtJ (001976) mice may be used as diabetes-susceptible controls.
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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