Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names NOR.NOD-(Il1-Ada)/Lt    (Changed: 28-DEC-06 ) NOR.NOD-(Il1-D2Mit144)/Lt    (Changed: 25-APR-06 ) NOR.NOD-Il1    (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain NOR/Lt Donor Strain NOD/ShiLt H2 Haplotype g7   Donating Investigator Edward Leiter,   The Jackson Laboratory

Appearance
albino
Related Genotype: Tyr^c/Tyr^c

Description
This NOR/Lt congenic strain is carrying alleles derived from strain NOD/Lt for interleukin 1a (Il1a^NOD), adenosine deaminase (Ada) and insulin dependent diabetes susceptibility 13 (Idd13^NOD) loci. Neither males nor females from this congenic strain when aged to 40 weeks showed diabetes development. This strain is useful for dissecting the role of non-MHC susceptibility genes in autoimmune diabetes.

Development
The congenic stock NOR.NOD-(Il1-Ada)/Lt, carrying a defined segment of Chr 2 that includes the NOD/Lt insulin dependent diabetes susceptibility locus Idd13 (71cM), was produced by mating mice from NOR/Lt to NOD/Lt followed by crossing 20 times to NOR/Lt with selection of NOD Il1 (94cM) genotype at each generation. An additional genome wide scan verified NOR homozygosity at all other chromosomal regions known to differ between NOD/Lt and NOR/Lt. This congenic strain retains NOR/Lt genome from D2Mit277 (69.0cM) through D2Mit395 (66.9cM) . The Il1-Ada region of Chr 2 in NOR/Lt was originally derived from the C57BL/6 contribution to strain C57BLKS/J; thus, this congenic strain retains B6 alleles for the microsatellite markers D2Mit277 through D2Mit395, including the functional gene B2m (69.0cM) (Reifsnyder et al, 2005). In 2006, the T1DR received this strain at N21F28.

Control Information

	Control
	001976 NOD/ShiLtJ	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying   Idd13^NOD/ShiLt allele

003064

B6.NOD-(D2Mit274-D2Mit343)/J

View Strains carrying   Idd13^NOD/ShiLt     (1 strain)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)

Idd13^NOD/ShiLt related

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ MHC Congenics)

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Genes & Alleles

Gene & Allele Information

Gene Symbol and Name		Ada, adenosine deaminase
Chromosome		2
Gene Common Name(s)		MGC108610;
Gene Symbol and Name		Il1, interleukin 1 complex
Chromosome		2
Gene Common Name(s)		Il-1;
Allele Symbol		Idd13NOD/ShiLt
Allele Name		NOD/ShiLt
Allele Type		QTL
Strain of Origin		NOD/ShiLt
Gene Symbol and Name		Idd13, insulin dependent diabetes susceptibility 13
Chromosome		2

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Reifsnyder PC; Li R; Silveira PA; Churchill G; Serreze DV; Leiter EH. 2005. Conditioning the genome identifies additional diabetes resistance loci in Type I diabetes resistant NOR/Lt mice. Genes Immun 6(6):528-38. [PubMed: 16015371]  [MGI Ref ID J:100365]

Additional References

Idd13^NOD/ShiLt related

Matsuki N; Stanic AK; Embers ME; Van Kaer L; Morel L; Joyce S. 2003. Genetic dissection of V alpha 14J alpha 18 natural T cell number and function in autoimmune-prone mice. J Immunol 170(11):5429-37. [PubMed: 12759418]  [MGI Ref ID J:85823]

Serreze DV; Prochazka M; Reifsnyder PC; Bridgett MM; Leiter EH. 1994. Use of recombinant congenic and congenic strains of NOD mice to identify a new insulin-dependent diabetes resistance gene. J Exp Med 180(4):1553-8. [PubMed: 7931087]  [MGI Ref ID J:20685]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Type 1 Diabetes Repository collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	001976 NOD/ShiLtJ	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

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General Terms and Conditions

Contact information

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