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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Elizabeth Simpson, University of British Columbia Description
C57BL/6J-TgN(pPWL88puro)2Ems mice are transgenic for the puromycin resistance gene, puromycin N-acetyltransferase (pac). This strain is a tool for the production of puromycin resistant mouse embryonic fibroblasts (MEF's) for embryonic stem cell selection. Embryonic fibroblasts derived from these mice are resistant to 2 ug/ml puromycin. Homozygosity for the transgene is embryonic lethal, so the strain is maintained by hemizygous matings. MEF's from individual embryos should be drug tested before use.
Related Strains
Strains carrying other alleles of Pgk1
002354 C57BL/6J-Tg(pPWL512hyg)1Ems/J 003208 STOCK Tg(DR4)1Jae/J View Strains carrying other alleles of Pgk1 (2 strains)
003208 STOCK Tg(DR4)1Jae/J
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
Genetics Research (Mutagenesis and Transgenesis: Production of Targeted Mutations ("Knockouts"), feeder cells for ES cell lines)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(pPWL88puro)2Ems | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Elizabeth M Simpson | ||
| Allele Type | Transgenic (random, expressed) | ||
| Mutation Made By | Elizabeth Simpson, University of British Columbia | ||
| Strain of Origin | C57BL/6J | ||
| Expressed Gene | puro, puromycin resistance, | ||
| Promoter | Pgk1, phosphoglycerate kinase 1, rat | ||
| General Note | Embryonic fibroblasts derived from transgenic mice on a C57BL/6 background are resistant to 2 ug/ml puromycin. Homozygous transgenic embryos die before birth. | ||
| Molecular Note | The transgene contains a phosphoglycerate kinase 1 promoter and the bacterial puromycin resistance gene, puromycin N-acetyltransferase (pac). [MGI Ref ID J:92461] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Puromycin, SEP PCR, vers. 2
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Linnell ER; Lerner CP; Johnson KA; Leach CA; Ulrich TR; Rafferty WC; Simpson EM. 2001. Transgenic mice for the preparation of puromycin-resistant primary embryonic fibroblast feeder layers for embryonic stem cell selection. Mamm Genome 12(2):169-71. [PubMed: 11210188] [MGI Ref ID J:92461]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
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