Strain Name:

B6;129S7-Asgr2tm1Her/J

Stock Number:

002361

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Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating InvestigatorDr. Joachim Herz,   Univ of Texas Southwest Med Ctr Dallas

Description
Mice homozygous for the Asgr2tm1Her targeted mutation are viable and have no obvious phenotype. There is no detectable ASGR2 expression and a pronounced reduction in ASGR1 expression. Expression of Asgr1 mRNA is not affected. Homozygous mutant mice are unable to clear 125I labeled asialoorosmomucoid. Plasma desialylated glycoprotein levels are not increased. The plasma lipoprotein protein profile level of mice deficient in both LDLR and ASGR2 does not differ from the LDLR deficient strain arguing against a major role of ASGR in lipoprotein metabolism.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Asgr2tm1Her allele
002387   129-Asgr2tm1Her/J
View Strains carrying   Asgr2tm1Her     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Asgr2tm1Her/Asgr2+

        involves: 129S7/SvEvBrd * C57BL/6
  • homeostasis/metabolism phenotype
  • abnormal homeostasis
    • heterozygotes are overtly normal but express reduced levels of ASGR1 protein, and clear asialoorosomucoid with intermediate efficiency from their circulation relative to wild-type   (MGI Ref ID J:21380)
    • however, similar to wild-type mice, heterozygotes fail to clear non-desialylated (125)I-labeled orosomucoid   (MGI Ref ID J:21380)

Asgr2tm1Her/Asgr2tm1Her

        involves: 129S7/SvEvBrd * C57BL/6
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • homozygotes are viable, fertile, overtly normal, and display no significant accumulation of desialylated glycoproteins or chylomicron remnants in their plasma   (MGI Ref ID J:21380)
    • at 6 weeks to 6 months, homozygotes display normal levels of low density lipoprotein receptor-related protein 1 (LRP1)   (MGI Ref ID J:35142)
    • in the total absence of normal ASGR function, homozygotes exhibit normal serum concentrations of galactose-terminating glycoproteins; however, other cross-competing endogenous ASGR ligands accumulate in serum   (MGI Ref ID J:35142)
    • abnormal homeostasis
      • notably, homozygotes exhibit significantly reduced ASGR1 protein expression in the presence of normal ASGR1 mRNA levels   (MGI Ref ID J:21380)
      • although homozygotes still express some residual ASGR1 protein, they are completely unable to clear asialoorosomucoid from their circulation   (MGI Ref ID J:21380)
      • however, similar to wild-type mice, homozygotes fail to clear non-desialylated (125)I-labeled orosomucoid   (MGI Ref ID J:21380)
      • the residual ASGR1 is expressed at the hepatocellular surface but fails to effectively remove any one of three different radiolabeled ligands within 30 minutes; mutants show a low but detectable residual clearance of 125I-labeled glycoproteins at 4 hours (not mediated by residual ASGR1)   (MGI Ref ID J:35142)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Asgr2tm1Her/Asgr2tm1Her

        B6.129S7-Asgr2tm1Her
  • mortality/aging
  • increased sensitivity to induced morbidity/mortality
    • following infection with S. pneumoniae, mice exhibit decreased survival compared to similarly treated wild-type mice   (MGI Ref ID J:136946)
  • immune system phenotype
  • increased susceptibility to bacterial infection
    • following infection with S. pneumoniae, mice exhibit decreased survival compared to similarly treated wild-type mice   (MGI Ref ID J:136946)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Asgr2tm1Her related

Cancer Research
Genes Regulating Growth and Proliferation

Cardiovascular Research
Other

Metabolism Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Asgr2tm1Her
Allele Name targeted mutation 1, Joachim Herz
Allele Type Targeted (Null/Knockout)
Common Name(s) ASGP-R2-; HL-2 -;
Mutation Made ByDr. Joachim Herz,   Univ of Texas Southwest Med Ctr Dallas
Strain of Origin129S7/SvEvBrd-Hprt<+>
ES Cell Line NameAB1
ES Cell Line Strain129S7/SvEvBrd-Hprt<+>
Gene Symbol and Name Asgr2, asialoglycoprotein receptor 2
Chromosome 11
Gene Common Name(s) ASGP-R2; ASGPR2; Asgr; Asgr-2; CLEC4H2; HBXBP; HL-2; asialoglycoprotein receptor complex;
Molecular Note A neomycin resistance cassette was inserted into exon 2. Northern blot analysis demonstrated that the transcript was not detectable in liver RNA from homozygous mice. The protein was also not detected in liver membrane protein fractions derived from homozygous mice. [MGI Ref ID J:21380]

Genotyping

Genotyping Information

Genotyping Protocols

Asgr2tm1Her, Standard PCR
Asgr2, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Ishibashi S; Hammer RE; Herz J. 1994. Asialoglycoprotein receptor deficiency in mice lacking the minor receptor subunit. J Biol Chem 269(45):27803-6. [PubMed: 7961705]  [MGI Ref ID J:21380]

Additional References

Braun JR; Willnow TE; Ishibashi S; Ashwell G; Herz J. 1996. The major subunit of the asialoglycoprotein receptor is expressed on the hepatocellular surface in mice lacking the minor receptor subunit. J Biol Chem 271(35):21160-6. [PubMed: 8702886]  [MGI Ref ID J:35142]

Asgr2tm1Her related

Braun JR; Willnow TE; Ishibashi S; Ashwell G; Herz J. 1996. The major subunit of the asialoglycoprotein receptor is expressed on the hepatocellular surface in mice lacking the minor receptor subunit. J Biol Chem 271(35):21160-6. [PubMed: 8702886]  [MGI Ref ID J:35142]

Grewal PK; Uchiyama S; Ditto D; Varki N; Le DT; Nizet V; Marth JD. 2008. The Ashwell receptor mitigates the lethal coagulopathy of sepsis. Nat Med 14(6):648-55. [PubMed: 18488037]  [MGI Ref ID J:136946]

Rumjantseva V; Grewal PK; Wandall HH; Josefsson EC; Sorensen AL; Larson G; Marth JD; Hartwig JH; Hoffmeister KM. 2009. Dual roles for hepatic lectin receptors in the clearance of chilled platelets. Nat Med 15(11):1273-80. [PubMed: 19783995]  [MGI Ref ID J:154297]

Steirer LM; Park EI; Townsend RR; Baenziger JU. 2009. The asialoglycoprotein receptor regulates levels of plasma glycoproteins terminating with sialic acid alpha2,6-galactose. J Biol Chem 284(6):3777-83. [PubMed: 19075021]  [MGI Ref ID J:147795]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThis strain is maintained by homozygous sibling matings.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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