Strain Name:

B6.129P2-Camk2atm1Sva/J

Stock Number:

002362

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Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain 129P2 via E14TG2a ES cell line
 
Donating Investigator Alcino J. Silva,   University of California, Los Angeles

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Camk2atm1Sva targeted mutation are viable but are reported not to breed. Homozygous mutant mice show no obvious anatomical defects in the nervous system. Postsynaptic mechanisms are intact, but long-term hippocampal potentiation (LTP), an electrophysiological manifestation of increased synapse strength, is deficient. These mutant mice show specific impairment in spatial learning, indicating that LTP is important to spatial though not necessarily to non-spatial memory. In a more recent study heterozygotes displayed normal offensive aggression. Heterozygotes exhibit a decreased fear response and an increase in defensive aggression. Homozygotes (in which both copies of the gene are absent) exhibit more pronounced behavior suggesting a gene dosage effect.

Development
This targeted mutant was made in the Laboratory of Dr. Susumu Tonegawa at the Massachusetts Institute of Technology. A replacement-type targeting vector was used which resulted in the deletion of the entire inhibitory domain and five amino acids in the amino end of the calmodulin-binding domain. Targeting was done in E14 ES cells.

Control Information

  Control
   See control note: Wildtype mice from the colony or C57BL/6J mice (Stock No. 000664) may be used as controls.
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Camk2a     (21 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Camk2atm1Sva/Camk2a+

        involves: 129P2/OlaHsd * C57BL/6J
  • nervous system phenotype
  • increased cerebral infarction size
    • 32.0+/-8.1 mm3 compared to 38.28+/-11.0 mm3 in homozygotes and 18.8+/-5.1 mm3 in wild-type mice   (MGI Ref ID J:113259)
  • homeostasis/metabolism phenotype
  • increased cerebral infarction size
    • 32.0+/-8.1 mm3 compared to 38.28+/-11.0 mm3 in homozygotes and 18.8+/-5.1 mm3 in wild-type mice   (MGI Ref ID J:113259)

Camk2atm1Sva/Camk2atm1Sva

        involves: 129P2/OlaHsd * BALB/c
  • nervous system phenotype
  • *normal* nervous system phenotype
    • mice exhibit normal nervous system anatomy   (MGI Ref ID J:1421)
    • decreased paired-pulse facilitation   (MGI Ref ID J:1421)
    • reduced long term potentiation
      • 14 of 16 mice display no long term potentiation   (MGI Ref ID J:1421)
  • behavior/neurological phenotype
  • decreased anxiety-related response
    • mice appear more 'jumpy' than wild-type mice and frantically avoid human contact   (MGI Ref ID J:1421)
    • however, mice exhibit otherwise normal behavior   (MGI Ref ID J:1421)

Camk2atm1Sva/Camk2atm1Sva

        involves: 129P2/OlaHsd
  • nervous system phenotype
  • abnormal CNS synaptic transmission
    • short term potentiation is absent   (MGI Ref ID J:113007)
    • however, post-tetanic potentiation is normal   (MGI Ref ID J:113007)
    • reduced long term depression
      • long term depression is present but reduced compared to in wild-type mice   (MGI Ref ID J:113007)
    • reduced long term potentiation
      • mice exhibit little to no long term potentiation after a priming period of low-frequency stimulation unlike in wild-type mice   (MGI Ref ID J:113007)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Camk2atm1Sva related

Metabolism Research

Neurobiology Research
Behavioral and Learning Defects
Cortical Defects
Epilepsy

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Camk2atm1Sva
Allele Name targeted mutation 1, Alcino Silva
Allele Type Targeted (Null/Knockout)
Common Name(s) Camk2atm1; alpha-CaMKII-; alphaCaMKII-; alphaCaMKIInull;
Mutation Made By Alcino Silva,   University of California, Los Angeles
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Camk2a, calcium/calmodulin-dependent protein kinase II alpha
Chromosome 18
Gene Common Name(s) CAMKA; PK2CDD; PKCCD; R74975; alpha-CaMKII; expressed sequence R74975; mKIAA0968;
Molecular Note A 130 bp DNA segment encoding the inhibitory domain and five amino acids of the calmodulin-binding domain was replaced with a neomycin selection cassette. Northern blot analysis on brain RNA derived from homozygous mice confirmed that no detectable transcript is produced from this allele. Western blot analysis on forebrain samples from homozygous mice demonstrated that no detectable protein is expressed from this allele. [MGI Ref ID J:1421]

Genotyping

Genotyping Information

Genotyping Protocols

Camk2atm1Sva, Standard PCR
NEOTD (Generic Neo), Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Silva AJ; Stevens CF; Tonegawa S; Wang Y. 1992. Deficient hippocampal long-term potentiation in alpha-calcium-calmodulin kinase II mutant mice [see comments] Science 257(5067):201-6. [PubMed: 1378648]  [MGI Ref ID J:1421]

Additional References

Chen C; Rainnie DG; Greene RW; Tonegawa S. 1994. Abnormal fear response and aggressive behavior in mutant mice deficient for alpha-calcium-calmodulin kinase II [see comments] Science 266(5183):291-4. [PubMed: 7939668]  [MGI Ref ID J:20972]

Frankland PW; O'Brien C; Ohno M; Kirkwood A; Silva AJ. 2001. Alpha-CaMKII-dependent plasticity in the cortex is required for permanent memory. Nature 411(6835):309-13. [PubMed: 11357133]  [MGI Ref ID J:69586]

Glazewski S; Chen CM; Silva A; Fox K. 1996. Requirement for alpha-CaMKII in experience-dependent plasticity of the barrel cortex. Science 272(5260):421-3. [PubMed: 8602534]  [MGI Ref ID J:78836]

Kirkwood A; Silva A; Bear MF. 1997. Age-dependent decrease of synaptic plasticity in the neocortex of alphaCaMKII mutant mice. Proc Natl Acad Sci U S A 94(7):3380-3. [PubMed: 9096402]  [MGI Ref ID J:39398]

Silva AJ; Paylor R; Wehner JM; Tonegawa S. 1992. Impaired spatial learning in alpha-calcium-calmodulin kinase II mutant mice [see comments] Science 257(5067):206-11. [PubMed: 1321493]  [MGI Ref ID J:1422]

Camk2atm1Sva related

Chen C; Rainnie DG; Greene RW; Tonegawa S. 1994. Abnormal fear response and aggressive behavior in mutant mice deficient for alpha-calcium-calmodulin kinase II [see comments] Science 266(5183):291-4. [PubMed: 7939668]  [MGI Ref ID J:20972]

Frankland PW; Bontempi B; Talton LE; Kaczmarek L; Silva AJ. 2004. The involvement of the anterior cingulate cortex in remote contextual fear memory. Science 304(5672):881-3. [PubMed: 15131309]  [MGI Ref ID J:89991]

Frankland PW; O'Brien C; Ohno M; Kirkwood A; Silva AJ. 2001. Alpha-CaMKII-dependent plasticity in the cortex is required for permanent memory. Nature 411(6835):309-13. [PubMed: 11357133]  [MGI Ref ID J:69586]

Glazewski S; Chen CM; Silva A; Fox K. 1996. Requirement for alpha-CaMKII in experience-dependent plasticity of the barrel cortex. Science 272(5260):421-3. [PubMed: 8602534]  [MGI Ref ID J:78836]

Gordon JA; Cioffi D; Silva AJ; Stryker MP. 1996. Deficient plasticity in the primary visual cortex of alpha-calcium/calmodulin-dependent protein kinase II mutant mice. Neuron 17(3):491-9. [PubMed: 8816712]  [MGI Ref ID J:35373]

Kim KS; Kim H; Park SK; Han PL. 2012. The dorsal striatum expressing adenylyl cyclase-5 controls behavioral sensitivity of the righting reflex to high-dose ethanol. Brain Res 1489:27-36. [PubMed: 23063718]  [MGI Ref ID J:193552]

Kirkwood A; Silva A; Bear MF. 1997. Age-dependent decrease of synaptic plasticity in the neocortex of alphaCaMKII mutant mice. Proc Natl Acad Sci U S A 94(7):3380-3. [PubMed: 9096402]  [MGI Ref ID J:39398]

Licata SC; Schmidt HD; Pierce RC. 2004. Suppressing calcium/calmodulin-dependent protein kinase II activity in the ventral tegmental area enhances the acute behavioural response to cocaine but attenuates the initiation of cocaine-induced behavioural sensitization in rats. Eur J Neurosci 19(2):405-14. [PubMed: 14725635]  [MGI Ref ID J:96021]

Matsuo N; Yamasaki N; Ohira K; Takao K; Toyama K; Eguchi M; Yamaguchi S; Miyakawa T. 2009. Neural activity changes underlying the working memory deficit in alpha-CaMKII heterozygous knockout mice. Front Behav Neurosci 3:20. [PubMed: 19750198]  [MGI Ref ID J:180422]

Silva AJ; Paylor R; Wehner JM; Tonegawa S. 1992. Impaired spatial learning in alpha-calcium-calmodulin kinase II mutant mice [see comments] Science 257(5067):206-11. [PubMed: 1321493]  [MGI Ref ID J:1422]

Silva AJ; Rosahl TW; Chapman PF; Marowitz Z; Friedman E; Frankland PW; Cestari V; Cioffi D; Sudhof TC; Bourtchuladze R. 1996. Impaired learning in mice with abnormal short-lived plasticity. Curr Biol 6(11):1509-18. [PubMed: 8939606]  [MGI Ref ID J:146734]

Silva AJ; Wang Y; Paylor R; Wehner JM; Stevens CF; Tonegawa S. 1992. Alpha calcium/calmodulin kinase II mutant mice: deficient long-term potentiation and impaired spatial learning. Cold Spring Harb Symp Quant Biol 57:527-39. [PubMed: 1339689]  [MGI Ref ID J:15040]

Stevens CF; Tonegawa S; Wang Y. 1994. The role of calcium-calmodulin kinase II in three forms of synaptic plasticity. Curr Biol 4(8):687-93. [PubMed: 7953554]  [MGI Ref ID J:113007]

Waxham MN; Grotta JC; Silva AJ; Strong R; Aronowski J. 1996. Ischemia-induced neuronal damage: a role for calcium/calmodulin-dependent protein kinase II. J Cereb Blood Flow Metab 16(1):1-6. [PubMed: 8530541]  [MGI Ref ID J:113259]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3300.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4290.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   See control note: Wildtype mice from the colony or C57BL/6J mice (Stock No. 000664) may be used as controls.
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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