Strain Name: |
B6.129P2-Camk2atm1Sva/J |
|---|---|
Stock Number: |
002362 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Camk2a; Camk2atm1Sva; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Background Strain C57BL/6 Donor Strain 129P2 via E14TG2a ES cell line Donating Investigator Alcino Silva, University of California, Los Angeles Generation N16pN1 (29-MAY-05) Appearance
black
Related Genotype: a/aStrain Description
Mice homozygous for the Camk2atm1Sva targeted mutation are viable but are reported not to breed. Homozygous mutant mice show no obvious anatomical defects in the nervous system. Postsynaptic mechanisms are intact, but long-term hippocampal potentiation (LTP), an electrophysiological manifestation of increased synapse strength, is deficient. These mutant mice show specific impairment in spatial learning, indicating that LTP is important to spatial though not necessarily to non-spatial memory. In a more recent study heterozygotes displayed normal offensive aggression. Heterozygotes exhibit a decreased fear response and an increase in defensive aggression. Homozygotes (in which both copies of the gene are absent) exhibit more pronounced behavior suggesting a gene dosage effect.Strain Development
This targeted mutant was made in the Laboratory of Dr. Susumu Tonegawa at the Massachusetts Institute of Technology. A replacement-type targeting vector was used which resulted in the deletion of the entire inhibitory domain and five amino acids in the amino end of the calmodulin-binding domain. Targeting was done in E14 ES cells.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Camk2atm1Sva | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Alcino Silva | ||
| Common Name(s) | Camk2atm1; alpha-CaMKII-; alphaCaMKII-; alphaCaMKIInull; | ||
| Mutation Made By | Alcino Silva, University of California, Los Angeles | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Camk2a, calcium/calmodulin-dependent protein kinase II alpha | ||
| Chromosome | 18 | ||
| Gene Common Name(s) | CAMKA; CaMKII; KIAA0968; PK2CDD; PKCCD; R74975; alpha-CaMKII; expressed sequence R74975; mKIAA0968; | ||
| Molecular Note | A 130 bp DNA segment encoding the inhibitory domain and five amino acids of the calmodulin-binding domain was replaced with a neomycin selection cassette. Northern blot analysis on brain RNA derived from homozygous mice confirmed that no detectable transcript is produced from this allele. Western blot analysis on forebrain samples from homozygous mice demonstrated that no detectable protein is expressed from this allele. [MGI Ref ID J:1421] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Wildtype mice from the colony or C57BL/6J mice (Stock No. 000664) may be used as controls. | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
NEOTD (Generic Neo)
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying other alleles of Camk2a
007574 B6.Cg-Tg(Camk2a-Crebbp*)1364Tabe/J 005855 B6.Cg-Tg(Camk2a-Prkaca)426Tabe/J 005359 B6.Cg-Tg(Camk2a-cre)T29-1Stl/J 007004 B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ 003010 B6;CBA-Tg(Camk2a-tTA)1Mmay/J 004995 C3H-Tg(Camk2a-Creb1/ESR1)3Sva/J 006575 C57BL/6-Camk2atm1Vyb/J 006579 C57BL/6-Tg(Camk2a-Bdnf)A9Stl/J 005087 C57BL/6-Tg(Camk2a-IDE)1Selk/J 005086 C57BL/6-Tg(Camk2a-MME)3Selk/J View Strains carrying other alleles of Camk2a (10 strains)
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:Camk2atm1Sva related
Metabolism Research
Neurobiology Research
Behavioral and Learning Defects
Cortical Defects
Epilepsy
References
Selected Reference(s)
Additional ReferencesSilva AJ; Stevens CF; Tonegawa S; Wang Y. 1992. Deficient hippocampal long-term potentiation in alpha-calcium-calmodulin kinase II mutant mice [see comments] Science 257(5067):201-6. [PubMed: 1378648] [MGI Ref ID J:1421]
Price and Supply Information
| Strain Name: | B6.129P2-Camk2atm1Sva/J |
| Stock Number: | 002362 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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