Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Hemizygote         (Female x Male) Species laboratory mouse Background Strain C57BL/6 Donor Strain 129S6 via EK.CCE ES cell line Generation N13F16 (20-DEC-06)   Donating Investigator M. Dinauer,   Indiana University School of Medicine

Appearance
black
Related Genotype: a/a

Description
Chronic granulomatous disease (CGD) is a recessive disorder characterized by a defective phagocyte respiratory burst oxidase, life-threatening pyogenic infections and inflammatory granulomas. Gene targeting was used to generate mice with a null allele of the gene involved in X-linked CGD, which encodes the 91 kD subunit of the oxidase cytochrome b. Affected hemizygous male mice lack phagocyte superoxide production, manifest an increased susceptibility to infection with Staphylococcus aureus and Aspergillus fumigatus and have an altered inflammatory response in thioglycollate peritonitis. This animal model should aid in developing new treatments for CGD and in evaluating the role of phagocyte-derived oxidants in inflammation.

Control Information

	Control
	000664 C57BL/6J
		C57BL/6J (Stock No. 000664) mice may be used as controls.
Considerations for Choosing Controls

Additional Web Information

Congenic Nomenclature
Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms

Granulomatous Disease, Chronic, X-Linked; CGD - Models with phenotypic similarity to human disease where etiologies involve orthologs.1

1 Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Cybb^tm1Din/Cybb^tm1Din

        B6.129S-Cybb^tm1Din

• immune system phenotype
• *normal* immune system phenotype (MGI Ref ID J:113463)
  • mice exhibit a normal local Shwartman response namely thrombohemorrhagic vasculitis

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Cybb^tm1Din/Cybb^tm1Din

        involves: 129S/SvEv * C57BL/6

• digestive/alimentary phenotype
• *normal* digestive/alimentary phenotype (MGI Ref ID J:64232)
  • susceptibility to indomethacin-induced changes in mucosa permeability is significantly attenuated relative to wild-type controls or Rag2-deficient mice
• abnormal gastric mucosa morphology (MGI Ref ID J:64232)
  • when given 10-20 mg/kg indomethacin, mice have markedly less damage to integrity of the gastric mucosa than controls or Rag2-null mice which develop extensive hemorrhagic lesions of the gastric mucosa
• abnormal intestinal mucosa morphology (MGI Ref ID J:64232)
  • when given 10-20 mg/kg indomethacin, mice have markedly less damage to integrity of the small bowel mucosa than controls or Rag2-null mice which develop small nonhemorrhagic lesions in the jejunum
• immune system phenotype
• *normal* immune system phenotype (MGI Ref ID J:64232)
  • mice develop minimal inflammation in response to indomethacin treatement compared to wild-type controls or Rag2-null animals

Cybb^tm1Din/Y

        involves: 129S/SvEv * C57BL/6

• immune system phenotype
• abnormal immune system physiology (MGI Ref ID J:22868)
• abnormal macrophage physiology (MGI Ref ID J:22868)
  • macrophages lacked respiratory burst activity
• abnormal neutrophil physiology (MGI Ref ID J:22868)
  • neutrophils lacked respiratory burst activity
• increased inflammatory response (MGI Ref ID J:22868)
  • dysregulated inflammatory response; increased numbers of peritoneal exudate neutrophils in chemical peritonitis induced by thioglycollate
• increased susceptibility to infection (MGI Ref ID J:22868)
  • increased frequency of spontaneously occurring infections
• increased susceptibility to bacterial infection (MGI Ref ID J:22868)
  • increased susceptibility to S. aureus infection and A. fumigatus infection

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cybb^tm1Din related

Hematological Research
Immunological Defects
Neutrophil Defects

Immunology and Inflammation Research
Immunodeficiency (Chronic Granulomatous Disease)
Immunodeficiency (Macrophage defects)
Immunodeficiency (Neutrophil Defects)
Inflammation (Neutrophil defects)

Metabolism Research
Chronic Granulomatous Disease

Mouse/Human Gene Homologs
Granulomatous Disease, Chronic, X-Linked; CGD

Research Tools
Immunology and Inflammation Research (Macrophage Deficiency)
Immunology and Inflammation Research (neutrophil NADPH oxydase deficiency)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Cybbtm1Din
Allele Name		targeted mutation 1, Mary C Dinauer
Allele Type		Targeted (knock-out)
Common Name(s)		Cybb KO; Cybbtm1; Cybbtm1Din; Nox-2-; PHOX-; X-CGD; gp91-; gp91phox-; gp91phox; gp91phox-;
Mutation Made By		M. Dinauer,   Indiana University School of Medicine
Strain of Origin		129S/SvEv-Gpi1
ES Cell Line Name		CCE/EK.CCE
ES Cell Line Strain		129S/SvEv-Gpi1
Gene Symbol and Name		Cybb, cytochrome b-245, beta polypeptide
Chromosome		X
Gene Common Name(s)		C88302; CGD; Cgd; GP91-1; GP91-PHOX; GP91PHOX; NOX2; expressed sequence C88302; gp91phox; p91-PHOX;
Molecular Note		A neomycin resistance gene was inserted into exon 3. Western blot analysis on extracts of neutrophil-rich peritoneal exudate cells from hemizygous male mice confirmed that no detectable protein was expressed. [MGI Ref ID J:22868]

Genotyping

Genotyping Information

Genotyping Protocols

Cybb^tm1Din, STD PCR, vers. 2

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Pollock JD; Williams DA; Gifford MA; Li LL; Du X; Fisherman J; Orkin SH; Doerschuk CM; Dinauer MC. 1995. Mouse model of X-linked chronic granulomatous disease, an inherited defect in phagocyte superoxide production. Nat Genet 9(2):202-9. [PubMed: 7719350]  [MGI Ref ID J:22868]

Additional References

Gao HM; Liu B; Hong JS. 2003. Critical role for microglial NADPH oxidase in rotenone-induced degeneration of dopaminergic neurons. J Neurosci 23(15):6181-7. [PubMed: 12867501]  [MGI Ref ID J:84474]

Hayashi T; Rao SP; Takabayashi K; Van Uden JH; Kornbluth RS; Baird SM; Taylor MW; Carson DA; Catanzaro A; Raz E. 2001. Enhancement of Innate Immunity against Mycobacterium avium Infection by Immunostimulatory DNA Is Mediated by Indoleamine 2,3-Dioxygenase. Infect Immun 69(10):6156-64. [PubMed: 11553555]  [MGI Ref ID J:71646]

Jackson SH; Devadas S; Kwon J; Pinto LA; Williams MS. 2004. T cells express a phagocyte-type NADPH oxidase that is activated after T cell receptor stimulation. Nat Immunol 5(8):818-27. [PubMed: 15258578]  [MGI Ref ID J:91766]

Ko J; Gendron-Fitzpatrick A; Splitter GA. 2002. Susceptibility of IFN regulatory factor-1 and IFN consensus sequence binding protein-deficient mice to brucellosis. J Immunol 168(5):2433-40. [PubMed: 11859135]  [MGI Ref ID J:74725]

Yang S; Porter VA; Cornfield DN; Milla C; Panoskaltsis-Mortari A; Blazar BR; Haddad IY. 2001. Effects of oxidant stress on inflammation and survival of iNOS knockout mice after marrow transplantation. Am J Physiol Lung Cell Mol Physiol 281(4):L922-30. [PubMed: 11557596]  [MGI Ref ID J:72096]

Cybb^tm1Din related

Abramov AY; Jacobson J; Wientjes F; Hothersall J; Canevari L; Duchen MR. 2005. Expression and modulation of an NADPH oxidase in mammalian astrocytes. J Neurosci 25(40):9176-84. [PubMed: 16207877]  [MGI Ref ID J:123045]

Al-Shabrawey M; Rojas M; Sanders T; Behzadian A; El-Remessy A; Bartoli M; Parpia AK; Liou G; Caldwell RB. 2008. Role of NADPH oxidase in retinal vascular inflammation. Invest Ophthalmol Vis Sci 49(7):3239-44. [PubMed: 18378574]  [MGI Ref ID J:136896]

Anderson MM; Heinecke JW. 2003. Production of N(epsilon)-(carboxymethyl)lysine is impaired in mice deficient in NADPH oxidase: a role for phagocyte-derived oxidants in the formation of advanced glycation end products during inflammation. Diabetes 52(8):2137-43. [PubMed: 12882933]  [MGI Ref ID J:107193]

Aratani Y; Kura F; Watanabe H; Akagawa H; Takano Y; Suzuki K; Dinauer MC; Maeda N; Koyama H. 2002. Critical role of myeloperoxidase and nicotinamide adenine dinucleotide phosphate-oxidase in high-burden systemic infection of mice with Candida albicans. J Infect Dis 185(12):1833-7. [PubMed: 12085336]  [MGI Ref ID J:126007]

Archer SL; Reeve HL; Michelakis E; Puttagunta L; Waite R; Nelson DP; Dinauer MC; Weir EK. 1999. O2 sensing is preserved in mice lacking the gp91 phox subunit of NADPH oxidase. Proc Natl Acad Sci U S A 96(14):7944-9. [PubMed: 10393927]  [MGI Ref ID J:77781]

Balish E; Warner TF; Nicholas PJ; Paulling EE; Westwater C; Schofield DA. 2005. Susceptibility of germfree phagocyte oxidase- and nitric oxide synthase 2-deficient mice, defective in the production of reactive metabolites of both oxygen and nitrogen, to mucosal and systemic candidiasis of endogenous origin. Infect Immun 73(3):1313-20. [PubMed: 15731028]  [MGI Ref ID J:96573]

Banerjee R; Anguita J; Fikrig E. 2000. Granulocytic ehrlichiosis in mice deficient in phagocyte oxidase or inducible nitric oxide synthase. Infect Immun 68(7):4361-2. [PubMed: 10858261]  [MGI Ref ID J:90886]

Barry-Lane PA; Patterson C; van der Merwe M; Hu Z; Holland SM; Yeh ET; Runge MS. 2001. p47phox is required for atherosclerotic lesion progression in ApoE(-/-) mice. J Clin Invest 108(10):1513-22. [PubMed: 11714743]  [MGI Ref ID J:111638]

Beck PL; Xavier R; Lu N; Nanda NN; Dinauer M; Podolsky DK; Seed B. 2000. Mechanisms of NSAID-induced gastrointestinal injury defined using mutant mice Gastroenterology 119(3):699-705. [PubMed: 10982764]  [MGI Ref ID J:64232]

Becker JS; Adler A; Schneeberger A; Huang H; Wang Z; Walsh E; Koller A; Hintze TH. 2005. Hyperhomocysteinemia, a cardiac metabolic disease: role of nitric oxide and the p22phox subunit of NADPH oxidase. Circulation 111(16):2112-8. [PubMed: 15851618]  [MGI Ref ID J:109692]

Bendall JK; Cave AC; Heymes C; Gall N; Shah AM. 2002. Pivotal role of a gp91(phox)-containing NADPH oxidase in angiotensin II-induced cardiac hypertrophy in mice. Circulation 105(3):293-6. [PubMed: 11804982]  [MGI Ref ID J:103177]

Bingel SA. 2002. Pathology of a mouse model of x-linked chronic granulomatous disease. Contemp Top Lab Anim Sci 41(5):33-8. [PubMed: 12213046]  [MGI Ref ID J:79195]

Bjorgvinsdottir H; Ding C; Pech N; Gifford MA; Li LL; Dinauer MC. 1997. Retroviral-mediated gene transfer of gp91phox into bone marrow cells rescues defect in host defense against Aspergillus fumigatus in murine X-linked chronic granulomatous disease. Blood 89(1):41-8. [PubMed: 8978275]  [MGI Ref ID J:38041]

Blanchard TG; Yu F; Hsieh CL; Redline RW. 2003. Severe inflammation and reduced bacteria load in murine helicobacter infection caused by lack of phagocyte oxidase activity. J Infect Dis 187(10):1609-15. [PubMed: 12721941]  [MGI Ref ID J:120653]

Block ML; Li G; Qin L; Wu X; Pei Z; Wang T; Wilson B; Yang J; Hong JS. 2006. Potent regulation of microglia-derived oxidative stress and dopaminergic neuron survival: substance P vs. dynorphin. FASEB J 20(2):251-8. [PubMed: 16449797]  [MGI Ref ID J:105782]

Bonnett CR; Cornish EJ; Harmsen AG; Burritt JB. 2006. Early neutrophil recruitment and aggregation in the murine lung inhibit germination of Aspergillus fumigatus Conidia. Infect Immun 74(12):6528-39. [PubMed: 16920786]  [MGI Ref ID J:115978]

Breitbach K; Klocke S; Tschernig T; van Rooijen N; Baumann U; Steinmetz I. 2006. Role of Inducible Nitric Oxide Synthase and NADPH Oxidase in Early Control of Burkholderia pseudomallei Infection in Mice. Infect Immun 74(11):6300-6309. [PubMed: 17000727]  [MGI Ref ID J:113561]

Byrne JA; Grieve DJ; Bendall JK; Li JM; Gove C; Lambeth JD; Cave AC; Shah AM. 2003. Contrasting roles of NADPH oxidase isoforms in pressure-overload versus angiotensin II-induced cardiac hypertrophy. Circ Res 93(9):802-5. [PubMed: 14551238]  [MGI Ref ID J:115652]

Chen Z; Keaney JF Jr; Schulz E; Levison B; Shan L; Sakuma M; Zhang X; Shi C; Hazen SL; Simon DI. 2004. Decreased neointimal formation in Nox2-deficient mice reveals a direct role for NADPH oxidase in the response to arterial injury. Proc Natl Acad Sci U S A 101(35):13014-9. [PubMed: 15316118]  [MGI Ref ID J:92447]

Cheng P; Corzo CA; Luetteke N; Yu B; Nagaraj S; Bui MM; Ortiz M; Nacken W; Sorg C; Vogl T; Roth J; Gabrilovich DI. 2008. Inhibition of dendritic cell differentiation and accumulation of myeloid-derived suppressor cells in cancer is regulated by S100A9 protein. J Exp Med 205(10):2235-49. [PubMed: 18809714]  [MGI Ref ID J:140101]

Coffey MJ; Serezani CH; Phare SM; Flamand N; Peters-Golden M. 2007. NADPH oxidase deficiency results in reduced alveolar macrophage 5-lipoxygenase expression and decreased leukotriene synthesis. J Leukoc Biol 82(6):1585-91. [PubMed: 17761955]  [MGI Ref ID J:129968]

Cornish EJ; Hurtgen BJ; McInnerney K; Burritt NL; Taylor RM; Jarvis JN; Wang SY; Burritt JB. 2008. Reduced nicotinamide adenine dinucleotide phosphate oxidase-independent resistance to Aspergillus fumigatus in alveolar macrophages. J Immunol 180(10):6854-67. [PubMed: 18453606]  [MGI Ref ID J:134869]

Crandall H; Ma Y; Dunn DM; Sundsbak RS; Zachary JF; Olofsson P; Holmdahl R; Weis JH; Weiss RB; Teuscher C; Weis JJ. 2005. Bb2Bb3 Regulation of Murine Lyme Arthritis Is Distinct from Ncf1 and Independent of the Phagocyte Nicotinamide Adenine Dinucleotide Phosphate Oxidase. Am J Pathol 167(3):775-85. [PubMed: 16127156]  [MGI Ref ID J:100689]

Deng S; Kruger A; Kleschyov AL; Kalinowski L; Daiber A; Wojnowski L. 2007. Gp91phox-containing NAD(P)H oxidase increases superoxide formation by doxorubicin and NADPH. Free Radic Biol Med 42(4):466-73. [PubMed: 17275678]  [MGI Ref ID J:118370]

Dinauer MC; Deck MB; Unanue ER. 1997. Mice lacking reduced nicotinamide adenine dinucleotide phosphate oxidase activity show increased susceptibility to early infection with Listeria monocytogenes. J Immunol 158(12):5581-3. [PubMed: 9190903]  [MGI Ref ID J:40899]

Dinauer MC; Gifford MA; Pech N; Li LL; Emshwiller P. 2001. Variable correction of host defense following gene transfer and bone marrow transplantation in murine X-linked chronic granulomatous disease. Blood 97(12):3738-45. [PubMed: 11389011]  [MGI Ref ID J:69962]

Ebrahimian TG; Heymes C; You D; Blanc-Brude O; Mees B; Waeckel L; Duriez M; Vilar J; Brandes RP; Levy BI; Shah AM; Silvestre JS. 2006. NADPH oxidase-derived overproduction of reactive oxygen species impairs postischemic neovascularization in mice with type 1 diabetes. Am J Pathol 169(2):719-28. [PubMed: 16877369]  [MGI Ref ID J:111988]

Fan J; Frey RS; Rahman A; Malik AB. 2002. Role of neutrophil NADPH oxidase in the mechanism of tumor necrosis factor-alpha -induced NF-kappa B activation and intercellular adhesion molecule-1 expression in endothelial cells. J Biol Chem 277(5):3404-11. [PubMed: 11729200]  [MGI Ref ID J:74318]

Feng HM; Walker DH. 2004. Mechanisms of immunity to Ehrlichia muris: a model of monocytotropic ehrlichiosis. Infect Immun 72(2):966-71. [PubMed: 14742542]  [MGI Ref ID J:87862]

Fresquet F; Pourageaud F; Leblais V; Brandes RP; Savineau JP; Marthan R; Muller B. 2006. Role of reactive oxygen species and gp91phox in endothelial dysfunction of pulmonary arteries induced by chronic hypoxia. Br J Pharmacol 148(5):714-23. [PubMed: 16715116]  [MGI Ref ID J:135722]

Fu XW; Wang D; Nurse CA; Dinauer MC; Cutz E. 2000. NADPH oxidase is an O2 sensor in airway chemoreceptors: evidence from K+ current modulation in wild-type and oxidase-deficient mice. Proc Natl Acad Sci U S A 97(8):4374-9. [PubMed: 10760304]  [MGI Ref ID J:61676]

Gao HM; Liu B; Hong JS. 2003. Critical role for microglial NADPH oxidase in rotenone-induced degeneration of dopaminergic neurons. J Neurosci 23(15):6181-7. [PubMed: 12867501]  [MGI Ref ID J:84474]

Gao XP; Standiford TJ; Rahman A; Newstead M; Holland SM; Dinauer MC; Liu QH; Malik AB. 2002. Role of NADPH oxidase in the mechanism of lung neutrophil sequestration and microvessel injury induced by Gram-negative sepsis: studies in p47phox-/- and gp91phox-/- mice. J Immunol 168(8):3974-82. [PubMed: 11937554]  [MGI Ref ID J:126179]

George-Chandy A; Nordstrom I; Nygren E; Jonsson IM; Postigo J; Collins LV; Eriksson K. 2008. Th17 development and autoimmune arthritis in the absence of reactive oxygen species. Eur J Immunol 38(4):1118-26. [PubMed: 18383034]  [MGI Ref ID J:133769]

Goebel WS; Mark LA; Billings SD; Meyers JL; Pech N; Travers JB; Dinauer MC. 2005. Gene correction reduces cutaneous inflammation and granuloma formation in murine X-linked chronic granulomatous disease. J Invest Dermatol 125(4):705-10. [PubMed: 16185269]  [MGI Ref ID J:101778]

Goebel WS; Pech NK; Dinauer MC. 2004. Stable long-term gene correction with low-dose radiation conditioning in murine X-linked chronic granulomatous disease. Blood Cells Mol Dis 33(3):365-71. [PubMed: 15528159]  [MGI Ref ID J:95009]

Graham DB; Stephenson LM; Lam SK; Brim K; Lee HM; Bautista J; Gilfillan S; Akilesh S; Fujikawa K; Swat W. 2007. An ITAM-signaling pathway controls cross-presentation of particulate but not soluble antigens in dendritic cells. J Exp Med 204(12):2889-97. [PubMed: 17984307]  [MGI Ref ID J:128521]

Grant AJ; Restif O; McKinley TJ; Sheppard M; Maskell DJ; Mastroeni P. 2008. Modelling within-host spatiotemporal dynamics of invasive bacterial disease. PLoS Biol 6(4):e74. [PubMed: 18399718]  [MGI Ref ID J:136287]

Haque MZ; Majid DS. 2004. Assessment of renal functional phenotype in mice lacking gp91PHOX subunit of NAD(P)H oxidase. Hypertension 43(2):335-40. [PubMed: 14718366]  [MGI Ref ID J:101981]

Haque MZ; Majid DS. 2008. Reduced renal responses to nitric oxide synthase inhibition in mice lacking the gene for gp91phox subunit of NAD(P)H oxidase. Am J Physiol Renal Physiol 295(3):F758-64. [PubMed: 18596078]  [MGI Ref ID J:138704]

Harraz MM; Marden JJ; Zhou W; Zhang Y; Williams A; Sharov VS; Nelson K; Luo M; Paulson H; Schoneich C; Engelhardt JF. 2008. SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. J Clin Invest 118(2):659-70. [PubMed: 18219391]  [MGI Ref ID J:131850]

Hayashi T; Yamashita C; Matsumoto C; Kwak CJ; Fujii K; Hirata T; Miyamura M; Mori T; Ukimura A; Okada Y; Matsumura Y; Kitaura Y. 2008. Role of gp91phox-containing NADPH oxidase in left ventricular remodeling induced by intermittent hypoxic stress. Am J Physiol Heart Circ Physiol 294(5):H2197-203. [PubMed: 18326795]  [MGI Ref ID J:135786]

He L; Chen J; Dinger B; Sanders K; Sundar K; Hoidal J; Fidone S. 2002. Characteristics of carotid body chemosensitivity in NADPH oxidase-deficient mice. Am J Physiol Cell Physiol 282(1):C27-33. [PubMed: 11742795]  [MGI Ref ID J:75608]

Hirahashi J; Mekala D; Van Ziffle J; Xiao L; Saffaripour S; Wagner DD; Shapiro SD; Lowell C; Mayadas TN. 2006. Mac-1 signaling via Src-family and Syk kinases results in elastase-dependent thrombohemorrhagic vasculopathy. Immunity 25(2):271-83. [PubMed: 16872848]  [MGI Ref ID J:113463]

Hung K; Hayashi R; Lafond-Walker A; Lowenstein C; Pardoll D; Levitsky H. 1998. The central role of CD4(+) T cells in the antitumor immune response. J Exp Med 188(12):2357-68. [PubMed: 9858522]  [MGI Ref ID J:51677]

Jackson SH; Devadas S; Kwon J; Pinto LA; Williams MS. 2004. T cells express a phagocyte-type NADPH oxidase that is activated after T cell receptor stimulation. Nat Immunol 5(8):818-27. [PubMed: 15258578]  [MGI Ref ID J:91766]

Johar S; Cave AC; Narayanapanicker A; Grieve DJ; Shah AM. 2006. Aldosterone mediates angiotensin II-induced interstitial cardiac fibrosis via a Nox2-containing NADPH oxidase. FASEB J 20(9):1546-8. [PubMed: 16720735]  [MGI Ref ID J:111378]

Jung YJ; LaCourse R; Ryan L; North RJ. 2002. Virulent but not avirulent Mycobacterium tuberculosis can evade the growth inhibitory action of a T helper 1-dependent, nitric oxide Synthase 2-independent defense in mice. J Exp Med 196(7):991-8. [PubMed: 12370260]  [MGI Ref ID J:119257]

Kassim SY; Fu X; Liles WC; Shapiro SD; Parks WC; Heinecke JW. 2005. NADPH oxidase restrains the matrix metalloproteinase activity of macrophages. J Biol Chem 280(34):30201-5. [PubMed: 15983040]  [MGI Ref ID J:101039]

Kazama K; Anrather J; Zhou P; Girouard H; Frys K; Milner TA; Iadecola C. 2004. Angiotensin II impairs neurovascular coupling in neocortex through NADPH oxidase-derived radicals. Circ Res 95(10):1019-26. [PubMed: 15499027]  [MGI Ref ID J:103853]

Kazemian P; Stephenson R; Yeger H; Cutz E. 2001. Respiratory control in neonatal mice with NADPH oxidase deficiency. Respir Physiol 126(2):89-101. [PubMed: 11348637]  [MGI Ref ID J:106249]

Keenan JI; Peterson RA 2nd; Hampton MB. 2005. NADPH oxidase involvement in the pathology of Helicobacter pylori infection. Free Radic Biol Med 38(9):1188-96. [PubMed: 15808416]  [MGI Ref ID J:97448]

Kim HS; Loughran PA; Rao J; Billiar TR; Zuckerbraun BS. 2008. Carbon monoxide activates NF-kappaB via ROS generation and Akt pathways to protect against cell death of hepatocytes. Am J Physiol Gastrointest Liver Physiol 295(1):G146-G152. [PubMed: 18497334]  [MGI Ref ID J:137836]

Kim YS; Choi DH; Block ML; Lorenzl S; Yang L; Kim YJ; Sugama S; Cho BP; Hwang O; Browne SE; Kim SY; Hong JS; Beal MF; Joh TH. 2007. A pivotal role of matrix metalloproteinase-3 activity in dopaminergic neuronal degeneration via microglial activation. FASEB J 21(1):179-87. [PubMed: 17116747]  [MGI Ref ID J:129758]

Kinugawa S; Zhang J; Messina E; Walsh E; Huang H; Kaminski PM; Wolin MS; Hintze TH. 2005. gp91phox-containing NAD(P)H oxidase mediates attenuation of nitric oxide-dependent control of myocardial oxygen consumption by ANG II. Am J Physiol Heart Circ Physiol 289(2):H862-7. [PubMed: 15778277]  [MGI Ref ID J:100315]

Kishida KT; Hoeffer CA; Hu D; Pao M; Holland SM; Klann E. 2006. Synaptic plasticity deficits and mild memory impairments in mouse models of chronic granulomatous disease. Mol Cell Biol 26(15):5908-20. [PubMed: 16847341]  [MGI Ref ID J:111410]

Ko J; Gendron-Fitzpatrick A; Splitter GA. 2002. Susceptibility of IFN regulatory factor-1 and IFN consensus sequence binding protein-deficient mice to brucellosis. J Immunol 168(5):2433-40. [PubMed: 11859135]  [MGI Ref ID J:74725]

Kobayashi T; Ogawa Y; Watanabe Y; Furuya M; Kataoka S; Garcia del Saz E; Tsunawaki S; Dinauer MC; Seguchi H. 2004. Mitochondrial transmembrane potential is diminished in phorbol myristate acetate-stimulated peritoneal resident macrophages isolated from wild-type mice, but not in those from gp91-phox-deficient mice. Histochem Cell Biol 122(4):323-32. [PubMed: 15243751]  [MGI Ref ID J:102741]

Kunz A; Abe T; Hochrainer K; Shimamura M; Anrather J; Racchumi G; Zhou P; Iadecola C. 2008. Nuclear factor-kappaB activation and postischemic inflammation are suppressed in CD36-null mice after middle cerebral artery occlusion. J Neurosci 28(7):1649-58. [PubMed: 18272685]  [MGI Ref ID J:132279]

Kurtz S; McKinnon KP; Runge MS; Ting JP; Braunstein M. 2006. The SecA2 secretion factor of Mycobacterium tuberculosis promotes growth in macrophages and inhibits the host immune response. Infect Immun 74(12):6855-64. [PubMed: 17030572]  [MGI Ref ID J:116072]

Li J; Baud O; Vartanian T; Volpe JJ; Rosenberg PA. 2005. Peroxynitrite generated by inducible nitric oxide synthase and NADPH oxidase mediates microglial toxicity to oligodendrocytes. Proc Natl Acad Sci U S A 102(28):9936-41. [PubMed: 15998743]  [MGI Ref ID J:99857]

Li JM; Fan LM; George VT; Brooks G. 2007. Nox2 regulates endothelial cell cycle arrest and apoptosis via p21(cip1) and p53. Free Radic Biol Med 43(6):976-86. [PubMed: 17697942]  [MGI Ref ID J:124355]

Liu JQ; Zelko IN; Erbynn EM; Sham JS; Folz RJ. 2006. Hypoxic pulmonary hypertension: role of superoxide and NADPH oxidase (gp91phox). Am J Physiol Lung Cell Mol Physiol 290(1):L2-10. [PubMed: 16085672]  [MGI Ref ID J:104796]

Liu JQ; Zelko IN; Folz RJ. 2004. Reoxygenation-induced constriction in murine coronary arteries: the role of endothelial NADPH oxidase (gp91phox) and intracellular superoxide. J Biol Chem 279(23):24493-7. [PubMed: 15070892]  [MGI Ref ID J:123986]

Lo W; Bravo T; Jadhav V; Titova E; Zhang JH; Tang J. 2007. NADPH oxidase inhibition improves neurological outcomes in surgically-induced brain injury. Neurosci Lett 414(3):228-32. [PubMed: 17317004]  [MGI Ref ID J:119831]

Lomas-Neira J; Chung CS; Perl M; Gregory S; Biffl W; Ayala A. 2006. Role of alveolar macrophage and migrating neutrophils in hemorrhage-induced priming for ALI subsequent to septic challenge. Am J Physiol Lung Cell Mol Physiol 290(1):L51-8. [PubMed: 16157517]  [MGI Ref ID J:104798]

Mastroeni P; Vazquez-Torres A; Fang FC; Xu Y; Khan S; Hormaeche CE; Dougan G. 2000. Antimicrobial actions of the NADPH phagocyte oxidase and inducible nitric oxide synthase in experimental salmonellosis. II. Effects on microbial proliferation and host survival in vivo. J Exp Med 192(2):237-48. [PubMed: 10899910]  [MGI Ref ID J:63489]

Matsuzaki I; Chatterjee S; Debolt K; Manevich Y; Zhang Q; Fisher AB. 2005. Membrane depolarization and NADPH oxidase activation in aortic endothelium during ischemia reflect altered mechanotransduction. Am J Physiol Heart Circ Physiol 288(1):H336-43. [PubMed: 15331375]  [MGI Ref ID J:95576]

Maytin M; Siwik DA; Ito M; Xiao L; Sawyer DB; Liao R; Colucci WS. 2004. Pressure overload-induced myocardial hypertrophy in mice does not require gp91phox. Circulation 109(9):1168-71. [PubMed: 14981002]  [MGI Ref ID J:131488]

Meissner F; Molawi K; Zychlinsky A. 2008. Superoxide dismutase 1 regulates caspase-1 and endotoxic shock. Nat Immunol 9(8):866-72. [PubMed: 18604212]  [MGI Ref ID J:137865]

Milovanova T; Chatterjee S; Hawkins BJ; Hong N; Sorokina EM; Debolt K; Moore JS; Madesh M; Fisher AB. 2008. Caveolae are an essential component of the pathway for endothelial cell signaling associated with abrupt reduction of shear stress. Biochim Biophys Acta 1783(10):1866-75. [PubMed: 18573285]  [MGI Ref ID J:140780]

Milovanova T; Chatterjee S; Manevich Y; Kotelnikova I; Debolt K; Madesh M; Moore JS; Fisher AB. 2006. Lung endothelial cell proliferation with decreased shear stress is mediated by reactive oxygen species. Am J Physiol Cell Physiol 290(1):C66-76. [PubMed: 16107509]  [MGI Ref ID J:115726]

Mori M; Stokes KY; Vowinkel T; Watanabe N; Elrod JW; Harris NR; Lefer DJ; Hibi T; Granger DN. 2005. Colonic blood flow responses in experimental colitis: time course and underlying mechanisms. Am J Physiol Gastrointest Liver Physiol 289(6):G1024-9. [PubMed: 16081759]  [MGI Ref ID J:104795]

Murray HW; Lu CM; Brooks EB; Fichtl RE; DeVecchio JL; Heinzel FP. 2003. Modulation of T-cell costimulation as immunotherapy or immunochemotherapy in experimental visceral leishmaniasis. Infect Immun 71(11):6453-62. [PubMed: 14573667]  [MGI Ref ID J:86275]

Murray HW; Nathan CF. 1999. Macrophage microbicidal mechanisms in vivo: reactive nitrogen versus oxygen intermediates in the killing of intracellular visceral Leishmania donovani. J Exp Med 189(4):741-6. [PubMed: 9989990]  [MGI Ref ID J:110956]

Murray HW; Xiang Z; Ma X. 2006. Responses to Leishmania donovani in mice deficient in both phagocyte oxidase and inducible nitric oxide synthase. Am J Trop Med Hyg 74(6):1013-5. [PubMed: 16760512]  [MGI Ref ID J:135733]

Mutunga M; Graham S; De Hormaeche RD; Musson JA; Robinson JH; Mastroeni P; Khan CM; Hormaeche CE. 2004. Attenuated Salmonella typhimurium htrA mutants cause fatal infections in mice deficient in NADPH oxidase and destroy NADPH oxidase-deficient macrophage monolayers. Vaccine 22(29-30):4124-31. [PubMed: 15364466]  [MGI Ref ID J:105621]

Nguyen HX; Tidball JG. 2003. Null mutation of gp91phox reduces muscle membrane lysis during muscle inflammation in mice. J Physiol 553(Pt 3):833-41. [PubMed: 14555723]  [MGI Ref ID J:105498]

Nicholson SC; Grobmyer SR; Shiloh MU; Brause JE; Potter S; MacMicking JD; Dinauer MC; Nathan CF. 1999. Lethality of endotoxin in mice genetically deficient in the respiratory burst oxidase, inducible nitric oxide synthase, or both. Shock 11(4):253-8. [PubMed: 10220301]  [MGI Ref ID J:56229]

Okada F; Kobayashi M; Tanaka H; Kobayashi T; Tazawa H; Iuchi Y; Onuma K; Hosokawa M; Dinauer MC; Hunt NH. 2006. The role of nicotinamide adenine dinucleotide phosphate oxidase-derived reactive oxygen species in the acquisition of metastatic ability of tumor cells. Am J Pathol 169(1):294-302. [PubMed: 16816381]  [MGI Ref ID J:110181]

Ostanin DV; Barlow S; Shukla D; Grisham MB. 2007. NADPH oxidase but not myeloperoxidase protects lymphopenic mice from spontaneous infections. Biochem Biophys Res Commun 355(3):801-6. [PubMed: 17316569]  [MGI Ref ID J:118594]

Park L; Anrather J; Zhou P; Frys K; Pitstick R; Younkin S; Carlson GA; Iadecola C. 2005. NADPH oxidase-derived reactive oxygen species mediate the cerebrovascular dysfunction induced by the amyloid beta peptide. J Neurosci 25(7):1769-77. [PubMed: 15716413]  [MGI Ref ID J:98244]

Park L; Zhou P; Pitstick R; Capone C; Anrather J; Norris EH; Younkin L; Younkin S; Carlson G; McEwen BS; Iadecola C. 2008. Nox2-derived radicals contribute to neurovascular and behavioral dysfunction in mice overexpressing the amyloid precursor protein. Proc Natl Acad Sci U S A 105(4):1347-52. [PubMed: 18202172]  [MGI Ref ID J:131374]

Peng T; Lu X; Feng Q. 2005. Pivotal role of gp91phox-containing NADH oxidase in lipopolysaccharide-induced tumor necrosis factor-alpha expression and myocardial depression. Circulation 111(13):1637-44. [PubMed: 15795323]  [MGI Ref ID J:108990]

Petersen JE; Hiran TS; Goebel WS; Johnson C; Murphy RC; Azmi FH; Hood AF; Travers JB; Dinauer MC. 2002. Enhanced cutaneous inflammatory reactions to Aspergillus fumigatus in a murine model of chronic granulomatous disease. J Invest Dermatol 118(3):424-9. [PubMed: 11874480]  [MGI Ref ID J:75716]

Petnehazy T; Cooper D; Stokes KY; Russell J; Wood KC; Granger DN. 2006. Angiotensin II type 1 receptors and the intestinal microvascular dysfunction induced by ischemia and reperfusion. Am J Physiol Gastrointest Liver Physiol 290(6):G1203-10. [PubMed: 16469824]  [MGI Ref ID J:111088]

Potter SM; Mitchell AJ; Cowden WB; Sanni LA; Dinauer M; de Haan JB; Hunt NH. 2005. Phagocyte-derived reactive oxygen species do not influence the progression of murine blood-stage malaria infections. Infect Immun 73(8):4941-7. [PubMed: 16041008]  [MGI Ref ID J:100436]

Qian L; Wei SJ; Zhang D; Hu X; Xu Z; Wilson B; El-Benna J; Hong JS; Flood PM. 2008. Potent anti-inflammatory and neuroprotective effects of TGF-beta1 are mediated through the inhibition of ERK and p47phox-Ser345 phosphorylation and translocation in microglia. J Immunol 181(1):660-8. [PubMed: 18566433]  [MGI Ref ID J:137172]

Qin L; Liu Y; Wang T; Wei SJ; Block ML; Wilson B; Liu B; Hong JS. 2004. NADPH oxidase mediates lipopolysaccharide-induced neurotoxicity and proinflammatory gene expression in activated microglia. J Biol Chem 279(2):1415-21. [PubMed: 14578353]  [MGI Ref ID J:135116]

Rey FE; Li XC; Carretero OA; Garvin JL; Pagano PJ. 2002. Perivascular superoxide anion contributes to impairment of endothelium-dependent relaxation: role of gp91(phox). Circulation 106(19):2497-502. [PubMed: 12417549]  [MGI Ref ID J:103372]

Rocha FJ; Schleicher U; Mattner J; Alber G; Bogdan C. 2007. Cytokines, signaling pathways, and effector molecules required for the control of Leishmania (Viannia) braziliensis in mice. Infect Immun 75(8):3823-32. [PubMed: 17517868]  [MGI Ref ID J:123377]

Roy A; Rozanov C; Mokashi A; Daudu P; Al-mehdi AB; Shams H; Lahiri S. 2000. Mice lacking in gp91 phox subunit of NAD(P)H oxidase showed glomus cell [Ca(2+)](i) and respiratory responses to hypoxia. Brain Res 872(1-2):188-93. [PubMed: 10924691]  [MGI Ref ID J:63733]

Schappi M; Deffert C; Fiette L; Gavazzi G; Herrmann F; Belli D; Krause KH. 2008. Branched fungal beta-glucan causes hyperinflammation and necrosis in phagocyte NADPH oxidase-deficient mice. J Pathol 214(4):434-44. [PubMed: 18098349]  [MGI Ref ID J:131826]

Serezani CH; Aronoff DM; Jancar S; Mancuso P; Peters-Golden M. 2005. Leukotrienes enhance the bactericidal activity of alveolar macrophages against Klebsiella pneumoniae through the activation of NADPH oxidase. Blood 106(3):1067-75. [PubMed: 15718414]  [MGI Ref ID J:117309]

Shiloh MU; MacMicking JD; Nicholson S; Brause JE; Potter S; Marino M; Fang F; Dinauer M; Nathan C. 1999. Phenotype of mice and macrophages deficient in both phagocyte oxidase and inducible nitric oxide synthase. Immunity 10(1):29-38. [PubMed: 10023768]  [MGI Ref ID J:54749]

Shvedova AA; Kisin ER; Murray AR; Kommineni C; Castranova V; Fadeel B; Kagan VE. 2008. Increased accumulation of neutrophils and decreased fibrosis in the lung of NADPH oxidase-deficient C57BL/6 mice exposed to carbon nanotubes. Toxicol Appl Pharmacol 231(2):235-40. [PubMed: 18534653]  [MGI Ref ID J:140070]

Snelgrove RJ; Edwards L; Williams AE; Rae AJ; Hussell T. 2006. In the absence of reactive oxygen species, T cells default to a Th1 phenotype and mediate protection against pulmonary Cryptococcus neoformans infection. J Immunol 177(8):5509-16. [PubMed: 17015737]  [MGI Ref ID J:139435]

Souza HP; Laurindo FR; Ziegelstein RC; Berlowitz CO; Zweier JL. 2001. Vascular NAD(P)H oxidase is distinct from the phagocytic enzyme and modulates vascular reactivity control. Am J Physiol Heart Circ Physiol 280(2):H658-67. [PubMed: 11158964]  [MGI Ref ID J:107847]

Stadler K; Bonini MG; Dallas S; Duma D; Mason RP; Kadiiska MB. 2008. Direct evidence of iNOS-mediated in vivo free radical production and protein oxidation in acetone-induced ketosis. Am J Physiol Endocrinol Metab 295(2):E456-62. [PubMed: 18559982]  [MGI Ref ID J:139996]

Stokes KY; Russell JM; Jennings MH; Alexander JS; Granger DN. 2007. Platelet-associated NAD(P)H oxidase contributes to the thrombogenic phenotype induced by hypercholesterolemia. Free Radic Biol Med 43(1):22-30. [PubMed: 17561090]  [MGI Ref ID J:122384]

Swain SD; Wright TW; Degel PM; Gigliotti F; Harmsen AG. 2004. Neither neutrophils nor reactive oxygen species contribute to tissue damage during Pneumocystis pneumonia in mice. Infect Immun 72(10):5722-32. [PubMed: 15385471]  [MGI Ref ID J:93116]

TeKippe M; Harrison DE; Chen J. 2003. Expansion of hematopoietic stem cell phenotype and activity in Trp53-null mice. Exp Hematol 31(6):521-7. [PubMed: 12829028]  [MGI Ref ID J:115677]

Thompson RJ; Farragher SM; Cutz E; Nurse CA. 2002. Developmental regulation of O(2) sensing in neonatal adrenal chromaffin cells from wild-type and NADPH-oxidase-deficient mice. Pflugers Arch 444(4):539-48. [PubMed: 12136274]  [MGI Ref ID J:106185]

Tojo T; Ushio-Fukai M; Yamaoka-Tojo M; Ikeda S; Patrushev N; Alexander RW. 2005. Role of gp91phox (Nox2)-containing NAD(P)H oxidase in angiogenesis in response to hindlimb ischemia. Circulation 111(18):2347-55. [PubMed: 15867174]  [MGI Ref ID J:111596]

Touyz RM; Mercure C; He Y; Javeshghani D; Yao G; Callera GE; Yogi A; Lochard N; Reudelhuber TL. 2005. Angiotensin II-dependent chronic hypertension and cardiac hypertrophy are unaffected by gp91phox-containing NADPH oxidase. Hypertension 45(4):530-7. [PubMed: 15753233]  [MGI Ref ID J:134741]

Ueno S; Campbell J; Fausto N. 2006. Reactive oxygen species derived from NADPH oxidase system is not essential for liver regeneration after partial hepatectomy. J Surg Res 136(2):260-5. [PubMed: 17046793]  [MGI Ref ID J:117642]

Underhill DM; Rossnagle E; Lowell CA; Simmons RM. 2005. Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production. Blood 106(7):2543-50. [PubMed: 15956283]  [MGI Ref ID J:119368]

Uppington H; Menager N; Boross P; Wood J; Sheppard M; Verbeek S; Mastroeni P. 2006. Effect of immune serum and role of individual Fcgamma receptors on the intracellular distribution and survival of Salmonella enterica serovar Typhimurium in murine macrophages. Immunology 119(2):147-58. [PubMed: 16836651]  [MGI Ref ID J:118526]

Vazquez-Torres A; Jones-Carson J; Mastroeni P; Ischiropoulos H; Fang FC. 2000. Antimicrobial actions of the NADPH phagocyte oxidase and inducible nitric oxide synthase in experimental salmonellosis. I. Effects on microbial killing by activated peritoneal macrophages in vitro. J Exp Med 192(2):227-36. [PubMed: 10899909]  [MGI Ref ID J:63490]

Wang HD; Xu S; Johns DG; Du Y; Quinn MT; Cayatte AJ; Cohen RA. 2001. Role of NADPH oxidase in the vascular hypertrophic and oxidative stress response to angiotensin II in mice. Circ Res 88(9):947-53. [PubMed: 11349005]  [MGI Ref ID J:115614]

Weissmann N; Zeller S; Schafer RU; Turowski C; Ay M; Quanz K; Ghofrani HA; Schermuly RT; Fink L; Seeger W; Grimminger F. 2006. Impact of mitochondria and NADPH oxidases on acute and sustained hypoxic pulmonary vasoconstriction. Am J Respir Cell Mol Biol 34(4):505-13. [PubMed: 16357364]  [MGI Ref ID J:120187]

White JK; Mastroeni P; Popoff JF; Evans CA; Blackwell JM. 2005. Slc11a1-mediated resistance to Salmonella enterica serovar Typhimurium and Leishmania donovani infections does not require functional inducible nitric oxide synthase or phagocyte oxidase activity. J Leukoc Biol 77(3):311-20. [PubMed: 15601666]  [MGI Ref ID J:97453]

Wilkie RP; Vissers MC; Dragunow M; Hampton MB. 2007. A functional NADPH oxidase prevents caspase involvement in the clearance of phagocytic neutrophils. Infect Immun 75(7):3256-63. [PubMed: 17438039]  [MGI Ref ID J:122425]

Wipke BT; Allen PM. 2001. Essential role of neutrophils in the initiation and progression of a murine model of rheumatoid arthritis. J Immunol 167(3):1601-8. [PubMed: 11466382]  [MGI Ref ID J:120467]

Wolfort RM; Stokes KY; Granger DN. 2008. CD4+ T lymphocytes mediate hypercholesterolemia-induced endothelial dysfunction via a NAD(P)H oxidase-dependent mechanism. Am J Physiol Heart Circ Physiol 294(6):H2619-26. [PubMed: 18408127]  [MGI Ref ID J:136828]

Woo H; Okamoto S; Guiney D; Gunn JS; Fierer J. 2008. A Model of Salmonella Colitis with Features of Diarrhea in SLC11A1 Wild-Type Mice. PLoS ONE 3(2):e1603. [PubMed: 18270590]  [MGI Ref ID J:132186]

Wood KC; Hebbel RP; Granger DN. 2005. Endothelial cell NADPH oxidase mediates the cerebral microvascular dysfunction in sickle cell transgenic mice. FASEB J 19(8):989-91. [PubMed: 15923406]  [MGI Ref ID J:128246]

Wu DC; Re DB; Nagai M; Ischiropoulos H; Przedborski S. 2006. The inflammatory NADPH oxidase enzyme modulates motor neuron degeneration in amyotrophic lateral sclerosis mice. Proc Natl Acad Sci U S A 103(32):12132-7. [PubMed: 16877542]  [MGI Ref ID J:111782]

Wuthrich M; Filutowicz HI; Warner T; Klein BS. 2002. Requisite elements in vaccine immunity to Blastomyces dermatitidis: plasticity uncovers vaccine potential in immune-deficient hosts. J Immunol 169(12):6969-76. [PubMed: 12471131]  [MGI Ref ID J:118419]

Yang S; Porter VA; Cornfield DN; Milla C; Panoskaltsis-Mortari A; Blazar BR; Haddad IY. 2001. Effects of oxidant stress on inflammation and survival of iNOS knockout mice after marrow transplantation. Am J Physiol Lung Cell Mol Physiol 281(4):L922-30. [PubMed: 11557596]  [MGI Ref ID J:72096]

Yao H; Edirisinghe I; Yang SR; Rajendrasozhan S; Kode A; Caito S; Adenuga D; Rahman I. 2008. Genetic ablation of NADPH oxidase enhances susceptibility to cigarette smoke-induced lung inflammation and emphysema in mice. Am J Pathol 172(5):1222-37. [PubMed: 18403597]  [MGI Ref ID J:134306]

Zhang Q; Matsuzaki I; Chatterjee S; Fisher AB. 2005. Activation of endothelial NADPH oxidase during normoxic lung ischemia is KATP channel dependent. Am J Physiol Lung Cell Mol Physiol 289(6):L954-61. [PubMed: 16280460]  [MGI Ref ID J:105015]

van de Loo FA; Bennink MB; Arntz OJ; Smeets RL; Lubberts E; Joosten LA; van Lent PL; Coenen-de Roo CJ; Cuzzocrea S; Segal BH; Holland SM; van den Berg WB. 2003. Deficiency of NADPH oxidase components p47phox and gp91phox caused granulomatous synovitis and increased connective tissue destruction in experimental arthritis models. Am J Pathol 163(4):1525-37. [PubMed: 14507659]  [MGI Ref ID J:109364]

von Loewenich FD; Scorpio DG; Reischl U; Dumler JS; Bogdan C. 2004. Frontline: control of Anaplasma phagocytophilum, an obligate intracellular pathogen, in the absence of inducible nitric oxide synthase, phagocyte NADPH oxidase, tumor necrosis factor, Toll-like receptor (TLR)2 and TLR4, or the TLR adaptor molecule MyD88. Eur J Immunol 34(7):1789-97. [PubMed: 15214027]  [MGI Ref ID J:90727]

Health & husbandry

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Animal Health Reports

Room Number           AX30

Colony Maintenance

Mating System	Homozygote x Hemizygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

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Standard Supply	Level 4. Up to 10 mice. Larger quantities or custom orders arranged upon request. Expected delivery up to one to three months.
Supply Notes	Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available. Strains that must be genotyped are not available until five to seven weeks of age. This strain is included in the Induced Mutant Resource Colony collection.

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	000664 C57BL/6J
		C57BL/6J (Stock No. 000664) mice may be used as controls.
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.