Strain Name:

C.129S4(B6)-Inhbbtm1Jae/J

Stock Number:

002368

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain BALB/c
Donor Strain B6,129S-Inhbbtm1Jae (129S4 derived J1 ES cell line)
 
Donating InvestigatorDr. Rudolf Jaenisch,   Whitehead Institute (MIT)

Description
Homozygous mice are deficient in activin B (betaB:betaB), activin AB (betaA:betaB) and inhibin B (alpha:betaB). Heterozygotes appear normal. Homozygous mutants complete embryonic development and are viable. They are born, however, with open eyes which leads to the development of eye lesions. Homozygous males breed normally but mutant females exhibit a profoundly impaired reproductive ability, characterized by perinatal lethality of their offspring. Mutant females have an increased gestation time and decreased nursing ability that may be the result of impaired milk let-down. There is an upregulation of beta-A activin which may contribute to the phenotype.

Control Information

  Control
   See control note: BALB/cJ mice (Stock No. 000651) may be used as controls.
   Wild-type from the colony
   000651 BALB/cJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Inhbbtm1Jae allele
002323   129S4/SvJae-Inhbbtm1Jae/J
002442   B6.129S4-Inhbbtm1Jae/J
View Strains carrying   Inhbbtm1Jae     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Inhbbtm1Jae/Inhbbtm1Jae

        either: (129S4/SvJae-Inhbbtm1Jae) or (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
  • mortality/aging
  • partial postnatal lethality
    • at weaning fewer than expected mice survive on a 129S4/SvJae BALB/c background compared to on a 124S4/SvJae C57BL/6 background with both exhibiting decreased survival relative to wild-type mice   (MGI Ref ID J:16995)
  • pregnancy-related premature death
    • in some females initiation of labor fails resulting in the death of the female and fetuses in utero   (MGI Ref ID J:16995)
  • reproductive system phenotype
  • long gestation period
    • most females deliver on E20.5 and some on E21.5 instead of E19.5 as wild-type females do   (MGI Ref ID J:16995)
  • reduced female fertility
    • female mice have a reduced ability to produce live offspring regardless of genotype to which they are mated   (MGI Ref ID J:16995)
    • female mice give birth to normal sized litters that die within the first day after birth regardless of genotype   (MGI Ref ID J:16995)
    • female mice with the 129S4/SvJae BALB/c background are most severely affected   (MGI Ref ID J:16995)
  • vision/eye phenotype
  • abnormal eye morphology
    • ocular dystrophy is observed in mice whose eyelids were delayed in opening   (MGI Ref ID J:16995)
    • abnormal corneal epithelium morphology
      • hyperkeratinization and squamous metaplasia of the corneal epithelium is observed during the first day after birth in mice with open eyelids   (MGI Ref ID J:16995)
    • corneal opacity
      • corneal opacification is observed in mice whose eyelids were delayed in opening   (MGI Ref ID J:16995)
    • delayed eyelid opening
      • eyelids that were open at birth and subsequently resealed exhibit a delay in reopening   (MGI Ref ID J:16995)
    • eyelids open at birth
      • some mice have open eye lids at birth but seal in subsequent days   (MGI Ref ID J:16995)
      • fewer mice on the 129S4/SvJae BALB/c background had open eyelids at birth compared to on the 129S4/SvJae C57BL/6 background and no mice on an inbred 129S4/SvJae background had open eyelids at birth   (MGI Ref ID J:16995)
    • increased incidence of corneal inflammation
      • increased inflammation of the cornea and eyelids is observed on the first day after birth in mice with open eyelids   (MGI Ref ID J:16995)
  • homeostasis/metabolism phenotype
  • increased circulating follicle stimulating hormone level
    • serum follicle stimulating hormone levels are increased by about 20% relative to in heterozygotes   (MGI Ref ID J:16995)
  • endocrine/exocrine gland phenotype
  • abnormal mammary gland morphology
    • at 0.5 days after delivery there is an increase in the glandular to adipose tissue ratio and vacuolation of the lobules of the mammary gland is observed   (MGI Ref ID J:16995)
  • abnormal milk ejection
    • maternally dependent fetal death is associated with a failure of milk to be ejected as pups fostered by wild-type mice survive   (MGI Ref ID J:16995)
    • milk accumulates in the mammary tissue at day 0.5 after delivery   (MGI Ref ID J:16995)
  • immune system phenotype
  • increased incidence of corneal inflammation
    • increased inflammation of the cornea and eyelids is observed on the first day after birth in mice with open eyelids   (MGI Ref ID J:16995)
  • integument phenotype
  • abnormal mammary gland morphology
    • at 0.5 days after delivery there is an increase in the glandular to adipose tissue ratio and vacuolation of the lobules of the mammary gland is observed   (MGI Ref ID J:16995)
  • abnormal milk ejection
    • maternally dependent fetal death is associated with a failure of milk to be ejected as pups fostered by wild-type mice survive   (MGI Ref ID J:16995)
    • milk accumulates in the mammary tissue at day 0.5 after delivery   (MGI Ref ID J:16995)
  • behavior/neurological phenotype
  • abnormal milk ejection
    • maternally dependent fetal death is associated with a failure of milk to be ejected as pups fostered by wild-type mice survive   (MGI Ref ID J:16995)
    • milk accumulates in the mammary tissue at day 0.5 after delivery   (MGI Ref ID J:16995)

Inhbbtm1Jae/Inhbbtm1Jae

        B6.129S4-Inhbbtm1Jae
  • muscle phenotype
  • abnormal pectoral muscle morphology
    • increased weight in males and females   (MGI Ref ID J:182827)
  • increased skeletal muscle weight
    • increased triceps weight in males   (MGI Ref ID J:182827)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Inhbbtm1Jae related

Cancer Research
Genes Regulating Growth and Proliferation

Developmental Biology Research
Eye Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads
      females only
Fertility Defects

Sensorineural Research
Eye Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Inhbbtm1Jae
Allele Name targeted mutation 1, Rudolf Jaenisch
Allele Type Targeted (Null/Knockout)
Common Name(s) Inhbb-; bb1; bb2;
Mutation Made ByDr. Rudolf Jaenisch,   Whitehead Institute (MIT)
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Inhbb, inhibin beta-B
Chromosome 1
Gene Common Name(s) activin;
Molecular Note A neomycin resistance cassette replaced a 1.3 kb region of the gene, which spanned the translational start codon, the signal peptide coding region, and most of exon 1 of the gene. In the construct designated bb1, the neomycin cassette was inserted in the same transcriptional orientation as the gene; in the construct designated bb2, the neomycin cassette was inserted in the reverse orientation. [MGI Ref ID J:16995]

Genotyping

Genotyping Information

Genotyping Protocols

Inhbbtm1Jae, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Vassalli A; Matzuk MM; Gardner HA; Lee KF; Jaenisch R. 1994. Activin/inhibin beta B subunit gene disruption leads to defects in eyelid development and female reproduction. Genes Dev 8(4):414-27. [PubMed: 8125256]  [MGI Ref ID J:16995]

Additional References

Inhbbtm1Jae related

Bertolino P; Holmberg R; Reissmann E; Andersson O; Berggren PO; Ibanez CF. 2008. Activin B receptor ALK7 is a negative regulator of pancreatic beta-cell function. Proc Natl Acad Sci U S A 105(20):7246-51. [PubMed: 18480258]  [MGI Ref ID J:136342]

Bonomi L; Brown M; Ungerleider N; Muse M; Matzuk MM; Schneyer A. 2012. Activin B regulates islet composition and islet mass but not whole body glucose homeostasis or insulin sensitivity. Am J Physiol Endocrinol Metab 303(5):E587-96. [PubMed: 22739106]  [MGI Ref ID J:189530]

Brown CW; Houston-Hawkins DE; Woodruff TK; Matzuk MM. 2000. Insertion of Inhbb into the Inhba locus rescues the Inhba-null phenotype and reveals new activin functions. Nat Genet 25(4):453-7. [PubMed: 10932194]  [MGI Ref ID J:63882]

Brown CW; Li L; Houston-Hawkins DE; Matzuk MM. 2003. Activins are critical modulators of growth and survival. Mol Endocrinol 17(12):2404-17. [PubMed: 14551263]  [MGI Ref ID J:86858]

Ferguson CA; Tucker AS; Christensen L; Lau AL; Matzuk MM; Sharpe PT. 1998. Activin is an essential early mesenchymal signal in tooth development that is required for patterning of the murine dentition. Genes Dev 12(16):2636-49. [PubMed: 9716414]  [MGI Ref ID J:49552]

Lee SJ; Lee YS; Zimmers TA; Soleimani A; Matzuk MM; Tsuchida K; Cohn RD; Barton ER. 2010. Regulation of muscle mass by follistatin and activins. Mol Endocrinol 24(10):1998-2008. [PubMed: 20810712]  [MGI Ref ID J:182827]

Li Q; Karam SM; Coerver KA; Matzuk MM; Gordon JI. 1998. Stimulation of activin receptor II signaling pathways inhibits differentiation of multiple gastric epithelial lineages. Mol Endocrinol 12(2):181-92. [PubMed: 9482661]  [MGI Ref ID J:110642]

Liu CF; Parker K; Yao HH. 2010. WNT4/beta-catenin pathway maintains female germ cell survival by inhibiting activin betaB in the mouse fetal ovary. PLoS One 5(4):e10382. [PubMed: 20454446]  [MGI Ref ID J:160549]

Matzuk MM; Kumar TR; Vassalli A; Bickenbach JR; Roop DR; Jaenisch R; Bradley A. 1995. Functional analysis of activins during mammalian development [see comments] Nature 374(6520):354-6. [PubMed: 7885473]  [MGI Ref ID J:23923]

Pangas SA. 2012. Regulation of the ovarian reserve by members of the transforming growth factor beta family. Mol Reprod Dev 79(10):666-79. [PubMed: 22847922]  [MGI Ref ID J:190579]

Pangas SA; Jorgez CJ; Tran M; Agno J; Li X; Brown CW; Kumar TR; Matzuk MM. 2007. Intraovarian activins are required for female fertility. Mol Endocrinol 21(10):2458-71. [PubMed: 17609433]  [MGI Ref ID J:125354]

Robinson GW; Hennighausen L. 1997. Inhibins and activins regulate mammary epithelial cell differentiation through mesenchymal-epithelial interactions. Development 124(14):2701-8. [PubMed: 9226441]  [MGI Ref ID J:41813]

Sandoval-Guzman T; Gongrich C; Moliner A; Guo T; Wu H; Broberger C; Ibanez CF. 2012. Neuroendocrine control of female reproductive function by the activin receptor ALK7. FASEB J 26(12):4966-76. [PubMed: 22954591]  [MGI Ref ID J:193340]

Shillingford JM; Miyoshi K; Robinson GW; Bierie B; Cao Y; Karin M; Hennighausen L. 2003. Proteotyping of mammary tissue from transgenic and gene knockout mice with immunohistochemical markers: a tool to define developmental lesions. J Histochem Cytochem 51(5):555-65. [PubMed: 12704203]  [MGI Ref ID J:124525]

Simmons DG; Cross JC. 2005. Determinants of trophoblast lineage and cell subtype specification in the mouse placenta. Dev Biol 284(1):12-24. [PubMed: 15963972]  [MGI Ref ID J:100602]

Wu H; Mezghenna K; Marmol P; Guo T; Moliner A; Yang SN; Berggren PO; Ibanez CF. 2014. Differential regulation of mouse pancreatic islet insulin secretion and Smad proteins by activin ligands. Diabetologia 57(1):148-56. [PubMed: 24132781]  [MGI Ref ID J:206532]

Yao HH; Aardema J; Holthusen K. 2006. Sexually dimorphic regulation of inhibin Beta B in establishing gonadal vasculature in mice. Biol Reprod 74(5):978-83. [PubMed: 16452457]  [MGI Ref ID J:107812]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3300.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4290.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   See control note: BALB/cJ mice (Stock No. 000651) may be used as controls.
   Wild-type from the colony
   000651 BALB/cJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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