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Strain Name:

B6D2-Tg(MMTVTGFA)29Rjc/J

Stock Number:

002373

Availability:

Repository-Cryopreserved

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Genes & Alleles   TGFA;   MMTV;   Tg(MMTVTGFA)29Rjc;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Strain
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Robert Coffey,   Vanderbilt University Medical Center
GenerationN?+2p+N1

Appearance
multiple coat colors
Related Genotype: segregating for Tyrp 1b and Myo5ad

Strain Description
Mice are viable and fertile, although mice of the most extensively studied line lactate insufficiently to support the litter. Virgin transgenic mice show no mammary gland abnormalities prepubertally, but adult virgin mice have considerable alveolar gland hyperplasia. Pregnant transgenic mice show marked proliferation of the stromal cells, and alveolar secretion is markedly increased compared to nontransgnic mice. After multiple pregnancies, isolated adenocarcinomas develop. There is no apparent phenotypic effect in males. In females, transgene expression is localized to the small ducts and alveoli in both virgin and pregnant mice as evidenced by in situ hybridization and by immunohistochemistry. Immunostaining also reveals some stromal staining in the hyperplastic areas. Egfr mRNA expression is also increased in mammary tissues expressing high levels of the transgene. Crosses between TGFA transgenic mice and TGFB1 transgenic mice demonstrate that these growth factors oppose each other's action on mammary gland development. The TGFA transgenic mice may also be useful in crosses with mice carrying mutations in the EGF and EGFR families. The putative tumor-promoter effect of TGFA may also be useful in enhancing the detection of tumor-initiating events and in determining the actions of putative metastases-inducing genes.

Mammalian Phenotype Terms assigned by genotype

Tg(MMTVTGFA)29Rjc/0

        involves: C57BL/6 * DBA/2
  • endocrine/exocrine gland phenotype
  • *normal* endocrine/exocrine gland phenotype (MGI Ref ID J:28453)
    • male mice exhibit normal mammary and salivary gland
    • abnormal mammary gland morphology (MGI Ref ID J:28453)
      • after formation of tumors at 3 months, glands are small with minimal secretion in the lumen and the stroma around the glands is abundant but mitosis and dysplasia of the epithelial or stromal cells is not observed
      • stroma between the mammary glands is hyperplastic
      • at 5 months two females develop bilateral swollen, lumpy thoracic mammary glands with abundant alveolar and ductal proliferation containing abundant secretions
      • at 5 months of age, female mice exhibit epithelial and stromal proliferation
      • the female founder mouse developed a mass during pregnancy that receded followed by development of masses in the inguinal fat pad and soft tissues of the upper thorax consisting of adenocarcinoma without metastasis
      • at 4 months of age, female mice develop numerous lumps within thoracic and inguinal areas of the mammary glands with large cystic spaces and abundant secretion within the lumen along with solid hyperplasias
      • female mice exhibit abnormal ductal trees
      • female mice exhibit cystically dilated alveoli
      • abnormal mammary gland growth during pregnancy (MGI Ref ID J:28453)
        • during pregnancy, female mice exhibit increased stromal cell proliferation, more prominent nuclei of epithelial cells, and production of more secretion than in wild type mice
        • after 3 month, female mice that have been pregnant develop an inguineal mammary fat pad that develops into a circumscribed, firm tumor
        • during pregnancy, female mice exhibit abnormal mammary gland hyperplasia with cysts that are more prominent and secretions that are more inspissated than in wild type mice
      • mammary alveolar hyperplasia (MGI Ref ID J:28453)
        • at day 90
      • mammary ductal hyperplasia (MGI Ref ID J:28453)
        • at 5 months of age
      • mammary gland hyperplasia (MGI Ref ID J:71696)
        • female mice exhibit lobular development similar to a lactating wild type mice except that the alveoli are irregular and dilated with interstitial stroma edema and a modest increase in leukocyte infiltration
        • however, nulliparous mice exhibit minimal lobular development and no stromal response
        • virgin females exhibit papillary hyperplasia
  • tumorigenesis
  • mammary gland tumor (MGI Ref ID J:28453)
    • after 3 month, female mice that have been pregnant develop an inguineal mammary fat pad that develops into a circumscribed, firm tumor
    • tumors have fibrous encapsulation with no evidence of local invasion and are composed of numerous well-formed alveoli with rare mitotic figures
    • adenomas develop in multiparous female mice
    • at 17 months, 53% of female mice exhibit solid tumors
    • in 6% of female mice at day 250
    • mammary adenocarcinoma (MGI Ref ID J:28453)
      • the female founder mouse developed a mass during pregnancy that receded followed by development of masses in the inguinal fat pad and soft tissues of the upper thorax consisting of adenocarcinoma without metastasis
      • solid hyperplasias develop into adenocarcinomas
      • in 40% of female mice
  • behavior/neurological phenotype
  • abnormal nursing (MGI Ref ID J:28453)
    • females cannot suckle her young
    • females cannot nurse her young
  • reproductive system phenotype
  • abnormal mammary gland morphology (MGI Ref ID J:28453)
    • after formation of tumors at 3 months, glands are small with minimal secretion in the lumen and the stroma around the glands is abundant but mitosis and dysplasia of the epithelial or stromal cells is not observed
    • stroma between the mammary glands is hyperplastic
    • at 5 months two females develop bilateral swollen, lumpy thoracic mammary glands with abundant alveolar and ductal proliferation containing abundant secretions
    • at 5 months of age, female mice exhibit epithelial and stromal proliferation
    • the female founder mouse developed a mass during pregnancy that receded followed by development of masses in the inguinal fat pad and soft tissues of the upper thorax consisting of adenocarcinoma without metastasis
    • at 4 months of age, female mice develop numerous lumps within thoracic and inguinal areas of the mammary glands with large cystic spaces and abundant secretion within the lumen along with solid hyperplasias
    • female mice exhibit abnormal ductal trees
    • female mice exhibit cystically dilated alveoli
    • abnormal mammary gland growth during pregnancy (MGI Ref ID J:28453)
      • during pregnancy, female mice exhibit increased stromal cell proliferation, more prominent nuclei of epithelial cells, and production of more secretion than in wild type mice
      • after 3 month, female mice that have been pregnant develop an inguineal mammary fat pad that develops into a circumscribed, firm tumor
      • during pregnancy, female mice exhibit abnormal mammary gland hyperplasia with cysts that are more prominent and secretions that are more inspissated than in wild type mice
    • mammary alveolar hyperplasia (MGI Ref ID J:28453)
      • at day 90
    • mammary ductal hyperplasia (MGI Ref ID J:28453)
      • at 5 months of age
    • mammary gland hyperplasia (MGI Ref ID J:71696)
      • female mice exhibit lobular development similar to a lactating wild type mice except that the alveoli are irregular and dilated with interstitial stroma edema and a modest increase in leukocyte infiltration
      • however, nulliparous mice exhibit minimal lobular development and no stromal response
      • virgin females exhibit papillary hyperplasia

Gene & Allele Details

Allele Symbol Tg(MMTVTGFA)29Rjc
Allele Name transgene insertion 29, Robert J Coffey
Mutation Made By Robert Coffey,   Vanderbilt University Medical Center
Strain of Origin(C57BL/6 x DBA/2)F2
Expressed Gene TGFA, transforming growth factor, alpha, human
Promoter MMTV, Mouse Mammary Tumor Virus, MMTV
Molecular Note This transgene contains the mouse mammary tumor virus LTR and the normal allele of the human transforming growth factor alpha gene. The vector also contains exons 2 and 3 of the rabbit beta-globin gene. Lines 254, 257, 64 and 29 were produced. [MGI Ref ID J:28453]

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(MMTVTGFA)

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying other alleles of TGFA
002459   B6D2-Tg(MMTVTGFA)254Rjc/J
002953   FVB.Cg-Tg(MMTVTGFA)254Rjc/J
002421   FVB/N-Tg(MtTGFA)100Lmb/J
View Strains carrying other alleles of TGFA     (3 strains)

View Strains carrying other alleles of MMTV     (17 strains)

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence (Mammary Gland Tumors)

References

Selected Reference(s)

Matsui Y; Halter SA; Holt JT; Hogan BL; Coffey RJ. 1990. Development of mammary hyperplasia and neoplasia in MMTV-TGF alpha transgenic mice. Cell 61(6):1147-55. [PubMed: 2161707]  [MGI Ref ID J:28453]

Additional References

Price and Supply Information

Strain Name: B6D2-Tg(MMTVTGFA)29Rjc/J
Stock Number: 002373

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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