Strain Name: |
B6Ros.Cg-Dmdmdx-5Cv/J |
|---|---|
Stock Number: |
002379 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Dmd; Dmdmdx-5Cv; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Species laboratory mouse Background Strain C57BL/6Ros Donor Strain C3Ha.X25 (Pgk1a Hprta) Donating Investigator Verne Chapman (deceased), Roswell Park Memorial Institute Strain Description
Mice carrying the Dmdmdx-5Cv mutation have 10 times fewer revertants than the Dmdmdx and Dmdmdx-2Cv strains as viewed in quadricep cross-sections. This is not attributable to genetic background or viral infections. These reversion rate differences may be attributable to differences in the location of the point mutation. The large number of revertants in Dmdmdx mutants has complicated the analysis of gene or cell therapies. These mutants are more useful for this purpose. This strain is also hemizygous for Hprta and Pgk1a (both are on the X chromosome).Strain Development
his strain was created in the laboratory of Verne M. Chapman. A C57BL/6Ros female was crossed to a male of strain C3Ha.X25, a double congenic strain carrying Pgk1a (from a wild Mus musculus musculus mouse trapped in Denmark) and Hprta (from Mus castaneus) on a C3H/HeHa background. F1 or F2 male progeny of this cross were treated with n-ethylnitrosourea (ENU) and crossed to C57BL/10Sn-Dmdmdx/+ females. Female offspring of these crosses that exhibited consistently elevated plasma creatine kinase levels and that carried the X-chromosome markers of their mutagenized male progenitors were bred to C57BL/10Sn-Dmdmdx/Y males. Transmission to male progeny of the elevated plasma CK phenotype and failure of the suspected new mutations at the Dmd locus to complement the classical mdx mutation identified four new mutations of Dmd, called Dmdmdx-2-5Cv . Each of these new mutations was subsequently backcrossed onto C57BL/6Ros.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Dmdmdx-5Cv | ||
|---|---|---|---|
| Allele Name | X linked muscular dystrophy 5, Verne Chapman | ||
| Common Name(s) | mdx5cv; mdx5cv; mdxCv5; | ||
| Mutation Made By | Verne Chapman (deceased), Roswell Park Memorial Institute | ||
| Strain of Origin | C3Ha.Cg-Hprt1 Pgk1 | ||
| Gene Symbol and Name | Dmd, dystrophin, muscular dystrophy | ||
| Chromosome | X | ||
| Gene Common Name(s) | BMD; CMD3B; DNADMD1; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; Dp427; Duchenne muscular dystrophy; RATDMD; X-linked muscular dystrophy; mdx; pke; pyruvate kinase expression; | ||
| Molecular Note | An A to T transversion at position 1324 in exon 10 creates a new splice donor site in the middle of exon 10. Aberrant splicing from the mutant site to exon 11 creates a 53 bp frameshifting deletion in the encoded mRNA. [MGI Ref ID J:34517] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | (approximate) | |
| Controls are provided from the C57BL/6J colony (Stock No. 000664). These only provide an approximate genetic match to this C57BL/6Ros background. | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Dmdmdx-5cv
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying other alleles of Dmd
002388 B6Ros.Cg-Dmdmdx-2Cv/J 002377 B6Ros.Cg-Dmdmdx-3Cv/J 002378 B6Ros.Cg-Dmdmdx-4Cv/J 001801 C57BL/10ScSn-Dmdmdx/J View Strains carrying other alleles of Dmd (4 strains)
Additional Web Information
Congenic Nomenclature
Genetic Quality Control Annual Report
Research Applications
This mouse can be used to support research in many areas including:Dmdmdx-5Cv related
Mouse/Human Gene Homologs
muscular dystrophy (Duchenne and Becker)
Neurobiology Research
Neuromuscular Defects
References
Selected Reference(s)
Additional ReferencesChapman VM; Miller DR; Armstrong D; Caskey CT. 1989. Recovery of induced mutations for X chromosome-linked muscular dystrophy in mice. Proc Natl Acad Sci U S A 86(4):1292-6. [PubMed: 2919177] [MGI Ref ID J:9638]
Price and Supply Information
| Strain Name: | B6Ros.Cg-Dmdmdx-5Cv/J |
| Stock Number: | 002379 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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