Strain Name: |
NOD.Cg-Tg(Ins2-TAg)1Lt Prkdcscid/DvsJ |
|---|---|
Stock Number: |
002380 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
NOD/Lt-Tg(RipTAg)1Lt Prkdcscid/DvsJ (Changed: 15-DEC-04
) |
| Genes & Alleles | Ins2; Prkdc; Prkdcscid; TAg; Tg(Ins2-TAg)1Lt; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator David Serreze, The Jackson Laboratory Generation F53pN1 Appearance
albino
Related Genotype: Tyrc/TyrcStrain Description
Homozygous NOD/Lt-TgN(RipTag)1Lt-Prkdcscid mice develop pancreatic beta cell adenomas. They are able to produce 2-3 litters before the insulin secreted from the adenoma makes them too hypoglycemic. Because Prkdcscid mice lack T-cells the adenomas from this strain are free of the autoimmune T-cells found in the adenomas of NOD/Lt-TgN(RipTag)1Lt mice.Strain Development
The TgN(RipTAg)1Lt transgene was generated in NOD/Lt. It was crossed with NOD.CB17-Prkdcscid by Dave Serreze and sibling bred to homozygosity for both the transgene and Prkdcscid.
Gene & Allele Details
| Allele Symbol | Prkdcscid | ||
|---|---|---|---|
| Allele Name | severe combined immunodeficiency | ||
| Common Name(s) | scid; | ||
| Strain of Origin | CB17 | ||
| Gene Symbol and Name | Prkdc, protein kinase, DNA activated, catalytic polypeptide | ||
| Chromosome | 16 | ||
| Gene Common Name(s) | AI326420; AU019811; DNA-PK; DNA-PKcs; DNAPDcs; DNAPK; DNPK1; HYRC; HYRC1; XRCC7; expressed sequence AI326420; expressed sequence AU019811; p350; scid; severe combined immunodeficiency; slip; | ||
| General Note |
The Prkdcscid mutation arose in the C.B-17 inbred strain (BALB/c.C57BL/Ka-Igh-1b) (J:9341). Most homozygotes have no detectable IgM, IgG1, IgG2a, IgG2b, IgG3, or IgA, but a few have low levels of one to three of these immunoglobulin isotypes. The size of the lymphoid organs is only one-tenth or less that of normal. Thymus, lymph nodes, and splenic follicles are virtually devoid of lymphocytes (J:30980). Homozygotes are deficient in both B and T cell function. Their spleen cells do not respond to either B or T cell mitogens and they are unable to reject skin grafts. They lack detectable B cells and pre-B cells. In spite of the small thymus and lack of functional T cells, the Thy1 marker is present on a majority of cells recovered from the thymus, and T cell lymphomas occur in 10 per cent or more of affected mice. Prkdcscid specifically impairs differentiation of stem cells into mature lymphocytes. Myeloid cell differentiation is not affected. The basic defect in these mice appears to be in the lymphoid stem cells and not in the cellular environment, since functional T and B cells are found in mice reconstituted with normal bone marrow (J:30980, J:7343). However, full reconstitution of the immune deficiency occurs only after irradiation of the recipients, indicating that Prkdcscid/Prkdcscid mice may have normal numbers of a radiation-sensitive stem cell that has defective proliferative capacity (J:8299). The rearrangements of immunoglobulin and T cell receptor genes that normally occur in B and T lymphocytes are not found in homozygous Prkdcscid mice. However, in Abelson leukemia virus-transformed B cells of these mice and in their occasional T cell lymphomas, rearrangements, most of which are abnormal, are found. This suggests that scid may act through an effect on the recombinase system catalyzing the assembly of immunoglobulin and T cell receptor genes, and that lymphocytes with these defects are not able to develop further (J:8420). Although most Prkdcscid homozygotes fail to produce immunoglobulin and functional T-cell receptor, some produce these products at low levels, with an occasional mouse with nearly normal levels of serum immunoglobulin, the criterion usually used tomeasure the effects of Prkdcscid. This phenomenon is referred to as "leakiness" of the VDJ recombination defect (J:4610).Homozygous Prkdcscidmice are fertile and, under specific pathogen-free conditions, may survive a year or more(J:6958). The Prkdcscid mouse has been widely used in studies of the immune system, in particular of VDJ recombination in T and B lymphocytes. Its lack of immunocompetence has made it useful in transplantation studies, particularly transplantation and development of metastasis in human tumors. The interaction of infection, immunity, and disease processes have been studied with these mice. Poole (J:31292) offers a brief review of the nature and usefulness of the Prkdcscid mouse, with key references to the very extensive literature. Mutant mRNA does not appear to differ from wild type although protein expression is reduced more than 10-fold. Mutant protein is defective for nuclear association but exhibits normal DNA-binding ability. NOD.Cg-Prkdcscid B2mtm1Unc mice lack mature lymphocytes and serum Ig, are MHC class I deficient, B and T cell deficient, C-5 deficient (Hc0), and have low NK cells. These mice display accumulation of iron in the liver and rapid clearance of human IgG1. | ||
| Molecular Note | A T-to-A transversion point mutation at a position corresponding to codon 4095 created a premature stop codon. [MGI Ref ID J:35393] [MGI Ref ID J:39329] | ||
| Allele Symbol | Tg(Ins2-TAg)1Lt | ||
| Allele Name | transgene insertion 1, Edward H Leiter | ||
| Common Name(s) | Tg(RipTAg)1Lt; | ||
| Mutation Made By | David Serreze, The Jackson Laboratory | ||
| Strain of Origin | NOD/ShiLt | ||
| Expressed Gene | TAg, SV40 large T-antigen, SV40 | ||
| Simian virus 40 T antigen (SV40Tag) is a multifunctional regulatory protein that stimulates gene transcription and forms complexes with cell cycle-regulatory proteins such as Trp53 and Rb1 that are implicated in human breast cancer. | |||
| Promoter | Ins2, insulin 2, rat | ||
| General Note | Homozygous transgenic mice on an NOD/Lt genetic background develop pancreatic beta cell adenomas. They are able to produce 2-3 litters before the insulin secreted from the adenoma makes them too hypoglycemic. | ||
| Molecular Note | The transgene contains rat insulin 2 promoter (Rip) and the wild-type allele of the SV40 large T antigen (TAg) gene. [MGI Ref ID J:92449] | ||
Control Information
| Allele | Control | |
|---|---|---|
| Prkdcscid | 001303 NOD.CB17-Prkdcscid/J | |
| Prkdcscid | 001976 NOD/ShiLtJ | |
| 001303 NOD.CB17-Prkdcscid/J | ||
| 001976 NOD/ShiLtJ | ||
| NOD/LtJ (Stock No. 001976) or NOD/LtSz-Prkdcscid/J mice (Stock No. 001303) may be used as controls. Additional control strains are available depending on the researchers needs. Please refer to JAX Notes No. 477 for a complete list of control strains available for NOD/LtJ mice in diabetes research. JAX Notes . | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Prkdcscid
TAg, SV
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, mice homozygous for the targeted mutation and hemizygous for the transgene (Tg/?) are bred together. This strain requires supplementation of drinking water with 5% sucrose to prevent hypoglycemia. Expected coat color from breeding: Albino. |
|---|
Related Strains
Strains carrying Prkdcscid allele
View Strains carrying Prkdcscid (25 strains)
002033 NOD/ShiLt-Tg(RipTAg)1Lt/J
008215 (C57BL/6-Tg(TRAMP)8247Ng/J X FVB/NJ)F1/J 003382 B10.D2-Tg(C3-1-TAg)cJeg/J 008247 B6.Cg-Tg(Ela1-TAg*)289Mjt/J 003380 B6.FVB-Tg(C3-1-TAg)cJeg/J 003135 C57BL/6-Tg(TRAMP)8247Ng/J 003445 C57BL/6J-Tg(Amy1TAg)354Knw/J 003446 C57BL/6J-Tg(Amy1TAg)501Knw/J 002233 C57BL/6J-Tg(SV)7Bri/J 003189 C57BL/6J-Tg(WAPTAg)3Knw/J 003188 C57BL/6J-Tg(WapTAg)1Knw 005564 FVB(Cg)-Tg(Ins2-CALM1)26Ove Tg(Cryaa-TAg)1Ove/PneJ 003381 FVB-Tg(C3-1-TAg)cJeg/J
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:
TAg relatedCancer Research
Increased Tumor Incidence (Adenomas: pancreatic b cells)
Diabetes and Obesity Research
Pancreatic B Cell Adenomas
Research Tools
Immunology and Inflammation Research (B and T cell deficiency)
Prkdcscid relatedApoptosis Research
Extracellular Modulators
Immunology and Inflammation Research
Immunodeficiency (B and T cell deficiency)
Internal/Organ Research
Lymphoid Tissue Defects (B and T cell deficiency)
Research Tools
Cancer Research (B and T cell deficiency) (xenograft/transplant host)
Toxicology Research (xenograft/transplant host)
Virology Research
B and T Cell Deficiency (AIDS research tool)
References
Selected Reference(s)
Additional ReferencesSerreze DV; Chapman HD; Varnum DS; Gerling I; Leiter EH; Shultz LD. 1997. Initiation of autoimmune diabetes in NOD/Lt mice is MHC class I-dependent. J Immunol 158(8):3978-86. [PubMed: 9103469] [MGI Ref ID J:39473]
Price and Supply Information
| Strain Name: | NOD.Cg-Tg(Ins2-TAg)1Lt Prkdcscid/DvsJ |
| Stock Number: | 002380 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
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