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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names FVB/N-Ucp(Dta) (Changed: 15-DEC-04 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation +N1 Appearance
albino
Related Genotype: Tyrc/TyrcImportant Note
This strain is homozygous for the retinal degeneration allele Pde6brd1.Description
Transgenic mice fed a "Western diet" developed marked obesity, insulin resistance, hyperglycemia, and hyperlipidemia. Mice hemizygous for the TgN(UcpDta)1Kz transgene develop early obesity in the absence of hyperphagia indicating an increased metabolic efficiency. Hyperphagia does follow and is present in mice over 7 weeks of age.Development
Brown adipose tissue was genetically ablated by incorpation of a toxic transgene, dipththeria toxin A-chain, coupled to regulatory elements of the gene for the brown adipose tissue specific uncoupling protein, UCP.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Pde6brd1 allele
View Strains carrying Pde6brd1 (74 strains)
008617 B6(A)-Tg(OPN1LW-DT)1Mame/J 008468 B6.Cg-Tg(tetO-DTA)1Gfi/J 006576 B6.FVB-Tg(GNAT2-Dta)98Wwk/J 008755 STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J 008168 STOCK Tg(tetO-DTA)1Gfi/J
004297 B6.CXB1-Pde6brd10/J 003647 B6EiC3Sn.BLiAF1 002802 C3.BLiA Pde6b+-Krd/J 001979 C3A.BLiA-Pde6b+.O20-Prph2Rd2/J 001912 C3A.BLiA-Pde6b+/J 003648 C3Sn.BLiA-Pde6b+/Dn 004766 C57BL/6J-Pde6brd1-2J/J 004828 FVB.129P2-Pde6b+ Tyrc-ch/AntJ 004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
002661 B6;SJL-Tg(EPO33)72Ptc/J
Phenotype
Phenotype Information
assigned by genotype
Tg(UcpDta)1Kz/?
involves: FVB/N
- growth/size phenotype
- increased body weight (MGI Ref ID J:78211)
- by 27 weeks, mice were nearly double the weight of controls
- obese (MGI Ref ID J:78211)
- evident at 16 days of age and progressive
- adipose tissue phenotype
- decreased brown adipose tissue amount (MGI Ref ID J:78211)
- lacked brown adipose tissue
- increased percent body fat (MGI Ref ID J:78211)
- by 19 weeks, mice had greater than 50% body fat
- homeostasis/metabolism phenotype
- *normal* homeostasis/metabolism phenotype (MGI Ref ID J:78211)
- normal corticosterone levels
- abnormal glucose homeostasis (MGI Ref ID J:78211)
- hyperglycemia (MGI Ref ID J:78211)
- increased circulating insulin level (MGI Ref ID J:78211)
- hyperinsulinemia
- insulin resistance (MGI Ref ID J:78211)
- abnormal lipid homeostasis (MGI Ref ID J:78211)
- hyperlipidemia (MGI Ref ID J:78211)
- increased circulating cholesterol level (MGI Ref ID J:78211)
- hypercholesterolemia
- increased triglyceride level (MGI Ref ID J:78211)
- hypertriglyceridemia
- increased circulating triglyceride level (MGI Ref ID J:147417)
- plasma triglyceride is dramatically increased over that of non-transgenic controls, and this can be reduced by hepatic overexpression of lipin 1
- behavior/neurological phenotype
- polyphagia (MGI Ref ID J:78211)
- beginning at 5-7 weeks
- nervous system phenotype
- *normal* nervous system phenotype (MGI Ref ID J:78211)
- histologically normal hypothalamus
This mouse can be used to support research in many areas including:
Pde6brd1 relatedDiabetes and Obesity Research
Hyperglycemia
diet-induced
Hyperinsulinemia
diet-induced
Insulin Resistance
diet-induced
Obesity With Diabetes
diet-induced
Type 2 Diabetes (NIDDM)
diet-induced
Metabolism Research
Mouse/Human Gene Homologs
retinitis pigmentosa, autosomal recessive
Sensorineural Research
Retinal Degeneration
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(UcpDta)1Kz | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Leslie P Kozak | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | BATless; UCP-DTA; | ||
| Strain of Origin | FVB/N | ||
| Expressed Gene | Dta, Diphtheria toxin A chain, | ||
| Brown fat specific expression of the A-chain of diptheria toxin (DTA) resulting in ablation of brown fat. | |||
| Promoter | Ucp, mitochondrial uncoupling protein, | ||
| Molecular Note | Incorporation of a toxic transgene, diptheria toxin-A-chain (Dta), coupled to regulatory elements of the gene for brown adipose tissue specific uncoupling protein (Ucp) resulted in the ablation of brown adipose tissue (BAT). Specifically, Dta was inserted into the first exon of Ucp1 at position +120 to create the vector. [MGI Ref ID J:78211] | ||
| Allele Symbol | Pde6brd1 | ||
| Allele Name | retinal degeneration 1 | ||
| Allele Type | Spontaneous | ||
| Common Name(s) | Pdebrd1; rd; rd-1; rd1; rodless retina; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(UcpDta)1Kz, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Lowell BB; S-Susulic V; Hamann A; Lawitts JA; Himms-Hagen J; Boyer BB; Kozak LP; Flier JS. 1993. Development of obesity in transgenic mice after genetic ablation of brown adipose tissue. Nature 366(6457):740-2. [PubMed: 8264795] [MGI Ref ID J:78211]
Additional References
Guerra C; Navarro P; Valverde AM; Arribas M; Bruning J; Kozak LP; Kahn CR; Benito M. 2001. Brown adipose tissue-specific insulin receptor knockout shows diabetic phenotype without insulin resistance. J Clin Invest 108(8):1205-13. [PubMed: 11602628] [MGI Ref ID J:72231]
Siri P; Candela N; Zhang YL; Ko C; Eusufzai S; Ginsberg HN; Huang LS. 2001. Post-transcriptional Stimulation of the Assembly and Secretion of Triglyceride-rich Apolipoprotein B Lipoproteins in a Mouse with Selective Deficiency of Brown Adipose Tissue, Obesity, and Insulin Resistance. J Biol Chem 276(49):46064-72. [PubMed: 11598138] [MGI Ref ID J:73122]
Tg(UcpDta)1Kz relatedChen Z; Gropler MC; Norris J; Lawrence JC Jr; Harris TE; Finck BN. 2008. Alterations in hepatic metabolism in fld mice reveal a role for lipin 1 in regulating VLDL-triacylglyceride secretion. Arterioscler Thromb Vasc Biol 28(10):1738-44. [PubMed: 18669885] [MGI Ref ID J:147417]
Cittadini A; Mantzoros CS; Hampton TG; Travers KE; Katz SE; Morgan JP; Flier JS; Douglas PS. 1999. Cardiovascular abnormalities in transgenic mice with reduced brown fat: an animal model of human obesity. Circulation 100(21):2177-83. [PubMed: 10571977] [MGI Ref ID J:59645]
Duncan JG; Fong JL; Medeiros DM; Finck BN; Kelly DP. 2007. Insulin-resistant heart exhibits a mitochondrial biogenic response driven by the peroxisome proliferator-activated receptor-alpha/PGC-1alpha gene regulatory pathway. Circulation 115(7):909-17. [PubMed: 17261654] [MGI Ref ID J:130544]
Finck BN; Bernal-Mizrachi C; Han DH; Coleman T; Sambandam N; LaRiviere LL; Holloszy JO; Semenkovich CF; Kelly DP. 2005. A potential link between muscle peroxisome proliferator- activated receptor-alpha signaling and obesity-related diabetes. Cell Metab 1(2):133-44. [PubMed: 16054054] [MGI Ref ID J:129848]
Fu L; John LM; Adams SH; Yu XX; Tomlinson E; Renz M; Williams PM; Soriano R; Corpuz R; Moffat B; Vandlen R; Simmons L; Foster J; Stephan JP; Tsai SP; Stewart TA. 2004. Fibroblast growth factor 19 increases metabolic rate and reverses dietary and leptin-deficient diabetes. Endocrinology 145(6):2594-603. [PubMed: 14976145] [MGI Ref ID J:133073]
Gouon-Evans V; Pollard JW. 2002. Unexpected deposition of brown fat in mammary gland during postnatal development. Mol Endocrinol 16(11):2618-27. [PubMed: 12403850] [MGI Ref ID J:132576]
Guerra C; Navarro P; Valverde AM; Arribas M; Bruning J; Kozak LP; Kahn CR; Benito M. 2001. Brown adipose tissue-specific insulin receptor knockout shows diabetic phenotype without insulin resistance. J Clin Invest 108(8):1205-13. [PubMed: 11602628] [MGI Ref ID J:72231]
Hamann A; Flier JS; Lowell BB. 1996. Decreased brown fat markedly enhances susceptibility to diet-induced obesity, diabetes, and hyperlipidemia. Endocrinology 137(1):21-9. [PubMed: 8536614] [MGI Ref ID J:100293]
Hollopeter G; Erickson JC; Palmiter RD. 1998. Role of neuropeptide Y in diet-, chemical- and genetic-induced obesity of mice. Int J Obes Relat Metab Disord 22(6):506-12. [PubMed: 9665670] [MGI Ref ID J:49932]
Klaus S; Munzberg H; Truloff C; Heldmaier G. 1998. Physiology of transgenic mice with brown fat ablation: obesity is due to lowered body temperature. Am J Physiol 274(2 Pt 2):R287-93. [PubMed: 9486283] [MGI Ref ID J:100346]
Mantzoros CS; Frederich RC; Qu D; Lowell BB; Maratos-Flier E; Flier JS. 1998. Severe leptin resistance in brown fat-deficient uncoupling protein promoter-driven diphtheria toxin A mice despite suppression of hypothalamic neuropeptide Y and circulating corticosterone concentrations [published erratum appears in Diabetes 1998 May;47(5):855] Diabetes 47(2):230-8. [PubMed: 9519718] [MGI Ref ID J:45532]
Melnyk A; Himms-Hagen J. 1998. Temperature-dependent feeding: lack of role for leptin and defect in brown adipose tissue-ablated obese mice. Am J Physiol 274(4 Pt 2):R1131-5. [PubMed: 9575979] [MGI Ref ID J:47074]
Ortmann S; Prinzler J; Klaus S. 2003. Self-selected macronutrient diet affects energy and glucose metabolism in brown fat-ablated mice. Obes Res 11(12):1536-44. [PubMed: 14694219] [MGI Ref ID J:100331]
Siri P; Candela N; Zhang YL; Ko C; Eusufzai S; Ginsberg HN; Huang LS. 2001. Post-transcriptional Stimulation of the Assembly and Secretion of Triglyceride-rich Apolipoprotein B Lipoproteins in a Mouse with Selective Deficiency of Brown Adipose Tissue, Obesity, and Insulin Resistance. J Biol Chem 276(49):46064-72. [PubMed: 11598138] [MGI Ref ID J:73122]
Vidal-Puig A; Jimenez-Linan M; Lowell BB; Hamann A; Hu E; Spiegelman B; Flier JS; Moller DE. 1996. Regulation of PPAR gamma gene expression by nutrition and obesity in rodents. J Clin Invest 97(11):2553-61. [PubMed: 8647948] [MGI Ref ID J:33358]
Zhang YL; Hernandez-Ono A; Siri P; Weisberg S; Conlon D; Graham MJ; Crooke RM; Huang LS; Ginsberg HN. 2006. Aberrant hepatic expression of PPARgamma2 stimulates hepatic lipogenesis in a mouse model of obesity, insulin resistance, dyslipidemia, and hepatic steatosis. J Biol Chem 281(49):37603-15. [PubMed: 16971390] [MGI Ref ID J:117583]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry Transgenic mice have micropthalamia and cataracts related to integration site. Transgenic animals can be identified at the age of 16 days. Expected coat color from breeding:Albino Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
|
| Important Note | |
| This strain is homozygous for the retinal degeneration allele Pde6brd1. | |
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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