Strain Name:

129-Cd5tm1Cgn/J

Stock Number:

002397

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Availability:

Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator Klaus Rajewsky,   Max-Delbruck-Ctr. for Molecular Medicine

Appearance
white-bellied agouti
Related Genotype: Aw/Aw

Description
Mice homozygous for the Cd5tm1Cgn targeted mutation are viable and apparently healthy. Both T and B cells in homozygous mutant mice lack surface CD5. Mice show normal immune responses to both T cell-dependent and -independent antigens.

Control Information

  Control
   000690 129P3/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Cd5
000408   B6.Cg-Cd5a/CyJ
001282   C3.B6/(86NS)CyJ
View Strains carrying other alleles of Cd5     (2 strains)

Additional Web Information

New 129 Nomenclature Bulletin

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Cd5tm1Cgn/Cd5tm1Cgn

        involves: 129P2/OlaHsd * C57BL/6
  • normal phenotype
  • no abnormal phenotype detected
    • mutant mice are viable, healthy and exhibit no changes in B and T cell populations, serum Ig content, and are able to mount effective T-cell dependent immune responses   (MGI Ref ID J:19317)

Cd5tm1Cgn/Cd5tm1Cgn

        B6.129P2-Cd5tm1Cgn
  • immune system phenotype
  • abnormal T cell anergy
    • T cells treated with anti-CD3 or MOG35-55 are resistant to development of anergy   (MGI Ref ID J:189919)
  • decreased susceptibility to experimental autoimmune encephalomyelitis
    • delayed onset and reduced severity of MOG-induced experimental autoimmune encephalomyelitis   (MGI Ref ID J:189919)
  • increased T cell proliferation
    • in CD4+ T cells stimulated with anti-CD3 or cognate peptide, rested then restimulated with anti-CD3 or MOG35-55   (MGI Ref ID J:189919)
    • in CD8+ T cells when restimulated   (MGI Ref ID J:189919)
  • increased regulatory T cell number   (MGI Ref ID J:189919)
  • hematopoietic system phenotype
  • increased T cell proliferation
    • in CD4+ T cells stimulated with anti-CD3 or cognate peptide, rested then restimulated with anti-CD3 or MOG35-55   (MGI Ref ID J:189919)
    • in CD8+ T cells when restimulated   (MGI Ref ID J:189919)
  • increased regulatory T cell number   (MGI Ref ID J:189919)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cd5tm1Cgn related

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Cd5tm1Cgn
Allele Name targeted mutation 1, University of Cologne
Allele Type Targeted (Null/Knockout)
Common Name(s) CD5-; CD50; CD5T;
Mutation Made By Alexander Tarakhovsky,   The Rockefeller University
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14.1
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Cd5, CD5 antigen
Chromosome 19
Gene Common Name(s) LEU1; Ly-1; Ly-12; Ly-A; Lyt-1; T-lymphocyte antigen 1; T1; lymphocyte antigen 1; lymphocyte antigen 12;
Molecular Note A neomycin gene replaced part of exon 7, which encodes the transmembrane domain. An additional frameshift mutation was introduced into exon 3. Flow cytometry analysis on cells derived from thymus, spleen and the peritoneal cavity of homozygous mice confirmed that no detectable protein was expressed from this allele. [MGI Ref ID J:19317]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Tarakhovsky A; Muller W; Rajewsky K. 1994. Lymphocyte populations and immune responses in CD5-deficient mice. Eur J Immunol 24(7):1678-84. [PubMed: 7517879]  [MGI Ref ID J:19317]

Additional References

Joliat MJ; Lang PA; Lyons BL; Burzenski L; Lynes MA; Yi T; Sundberg JP; Shultz LD. 2002. Absence of CD5 Dramatically Reduces Progression of Pulmonary Inflammatory Lesions in SHP-1 Protein-Tyrosine Phosphatase-Deficient 'Viable Motheaten' Mice. J Autoimmun 18(2):105-17. [PubMed: 11908943]  [MGI Ref ID J:76141]

Cd5tm1Cgn related

Axtell RC; Webb MS; Barnum SR; Raman C. 2004. Cutting edge: critical role for CD5 in experimental autoimmune encephalomyelitis: inhibition of engagement reverses disease in mice. J Immunol 173(5):2928-32. [PubMed: 15322150]  [MGI Ref ID J:92725]

Axtell RC; Xu L; Barnum SR; Raman C. 2006. CD5-CK2 binding/activation-deficient mice are resistant to experimental autoimmune encephalomyelitis: protection is associated with diminished populations of IL-17-expressing T cells in the central nervous system. J Immunol 177(12):8542-9. [PubMed: 17142752]  [MGI Ref ID J:140670]

Azzam HS; DeJarnette JB; Huang K; Emmons R; Park CS; Sommers CL; El-Khoury D; Shores EW; Love PE. 2001. Fine tuning of TCR signaling by CD5. J Immunol 166(9):5464-72. [PubMed: 11313384]  [MGI Ref ID J:106735]

Hayes SM; Li L; Love PE. 2005. TCR signal strength influences alphabeta/gammadelta lineage fate. Immunity 22(5):583-93. [PubMed: 15894276]  [MGI Ref ID J:99104]

Hippen KL; Tze LE; Behrens TW. 2000. CD5 maintains tolerance in anergic B cells. J Exp Med 191(5):883-90. [PubMed: 10704468]  [MGI Ref ID J:124695]

Joliat MJ; Lang PA; Lyons BL; Burzenski L; Lynes MA; Yi T; Sundberg JP; Shultz LD. 2002. Absence of CD5 Dramatically Reduces Progression of Pulmonary Inflammatory Lesions in SHP-1 Protein-Tyrosine Phosphatase-Deficient 'Viable Motheaten' Mice. J Autoimmun 18(2):105-17. [PubMed: 11908943]  [MGI Ref ID J:76141]

Laky K; Fowlkes BJ. 2007. Presenilins regulate alphabeta T cell development by modulating TCR signaling. J Exp Med 204(9):2115-29. [PubMed: 17698590]  [MGI Ref ID J:126088]

Li R; Page DM. 2001. Requirement for a complex array of costimulators in the negative selection of autoreactive thymocytes in vivo. J Immunol 166(10):6050-6. [PubMed: 11342622]  [MGI Ref ID J:110925]

Mizoguchi A; Mizoguchi E; de Jong YP; Takedatsu H; Preffer FI; Terhorst C; Bhan AK. 2003. Role of the CD5 molecule on TCR gammadelta T cell-mediated immune functions: development of germinal centers and chronic intestinal inflammation. Int Immunol 15(1):97-108. [PubMed: 12502730]  [MGI Ref ID J:110967]

Ordonez-Rueda D; Lozano F; Sarukhan A; Raman C; Garcia-Zepeda EA; Soldevila G. 2009. Increased numbers of thymic and peripheral CD4+ CD25+Foxp3+ cells in the absence of CD5 signaling. Eur J Immunol 39(8):2233-47. [PubMed: 19609976]  [MGI Ref ID J:151986]

Pena-Rossi C; Zuckerman LA; Strong J; Kwan J; Ferris W; Chan S; Tarakhovsky A; Beyers AD; Killeen N. 1999. Negative regulation of CD4 lineage development and responses by CD5. J Immunol 163(12):6494-501. [PubMed: 10586041]  [MGI Ref ID J:112133]

Sestero CM; McGuire DJ; De Sarno P; Brantley EC; Soldevila G; Axtell RC; Raman C. 2012. CD5-dependent CK2 activation pathway regulates threshold for T cell anergy. J Immunol 189(6):2918-30. [PubMed: 22904299]  [MGI Ref ID J:189919]

Tabbekh M; Franciszkiewicz K; Haouas H; Lecluse Y; Benihoud K; Raman C; Mami-Chouaib F. 2011. Rescue of tumor-infiltrating lymphocytes from activation-induced cell death enhances the antitumor CTL response in CD5-deficient mice. J Immunol 187(1):102-9. [PubMed: 21622855]  [MGI Ref ID J:175940]

Tarakhovsky A; Kanner SB; Hombach J; Ledbetter JA; Muller W; Killeen N; Rajewsky K. 1995. A role for CD5 in TCR-mediated signal transduction and thymocyte selection. Science 269(5223):535-7. [PubMed: 7542801]  [MGI Ref ID J:89249]

Teh SJ; Killeen N; Tarakhovsky A; Littman DR; Teh HS. 1997. CD2 regulates the positive selection and function of antigen-specific CD4- CD8+ T cells. Blood 89(4):1308-18. [PubMed: 9028954]  [MGI Ref ID J:111606]

Xu Z; Butfiloski EJ; Sobel ES; Morel L. 2004. Mechanisms of peritoneal B-1a cells accumulation induced by murine lupus susceptibility locus Sle2. J Immunol 173(10):6050-8. [PubMed: 15528340]  [MGI Ref ID J:132281]

Zou X; Smith JA; Corcos D; Matheson LS; Osborn MJ; Bruggemann M. 2008. Removal of the BiP-retention domain in Cmu permits surface deposition and developmental progression without L-chain. Mol Immunol 45(13):3573-9. [PubMed: 18584871]  [MGI Ref ID J:137831]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000690 129P3/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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