Strain Name:

B6;129P2-Csf3tm1Ard/J

Stock Number:

002398

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Other products are available, see Purchasing Information for Cryopreserved Embryos

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Ashley Dunn,   Ludwig Institute for Cancer Research

Appearance
Multiple coat colors.

Description
Mice homozygous for the Csf3tm1Ard targeted mutation are viable and fertile but are characterized by chronic neutropenia. Peripheral blood neutrophil levels of homozygotes are 20 to 30% of wildtype. There is a 50% reduction of granulocyte, macrophage and blast progenitor cells in marrow of homozygous mice and an impaired resistance to infection with Listeria monocytogenes.

Development
The Csf3-deficient strain was produced in the laboratory of Dr. Ashley Dunn from the Ludwig Institute for Cancer Research at The Royal Melbourne Hospital, Victoria, Australia. A replacement type targeting vector was used that resulted in the deletion of exons 1 and 2 and most of exon 3. E14 derived ES cells were used.

Control Information

  Control
   100903 B6129PF2/J (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Csf3tm1Ard/Csf3+

        involves: 129P2/OlaHsd * C57BL/6
  • hematopoietic system phenotype
  • decreased neutrophil cell number
    • heterozygotes exhibit a chronic neutropenia, with blood neutrophil levels reduced to ~67% of wild-type levels, suggesting a gene-dosage effect   (MGI Ref ID J:20400)
  • immune system phenotype
  • decreased neutrophil cell number
    • heterozygotes exhibit a chronic neutropenia, with blood neutrophil levels reduced to ~67% of wild-type levels, suggesting a gene-dosage effect   (MGI Ref ID J:20400)

Csf3tm1Ard/Csf3tm1Ard

        involves: 129P2/OlaHsd * C57BL/6
  • hematopoietic system phenotype
  • abnormal neutrophil physiology
    • homozygotes exhibit impaired neutrophil mobilization in response to a single dose of G-CSF, with only 21% of wild-type circulating neutrophil levels at 3 hrs post-treatment   (MGI Ref ID J:20400)
  • decreased leukocyte cell number
    • homozygotes are viable, fertile, and developmentally normal with no overt signs of disease or significant changes in hemoglobin or platelet levels   (MGI Ref ID J:20400)
    • however, homozygotes exhibit leukopenia as a result of significant selective neutropenia   (MGI Ref ID J:20400)
    • some homozygotes exhibit reduced circulating lymphocyte levels; however, these changes are inconsistent   (MGI Ref ID J:20400)
    • decreased monocyte cell number
      • homozygotes display significantly reduced monocyte levels relative to wild-type mice   (MGI Ref ID J:20400)
    • decreased neutrophil cell number
      • homozygotes exhibit a severe chronic neutropenia, with blood neutrophil levels reduced to ~30% of wild-type levels   (MGI Ref ID J:20400)
      • however, no significant changes in circulating eosinophil numbers are observed   (MGI Ref ID J:20400)
  • impaired myelopoiesis
    • homozygotes show a 50% decrease in granulopoietic precursor cells in the bone marrow, with reduced levels of granulocyte, macrophage, and blast progenitor cells   (MGI Ref ID J:20400)
    • G-CSF administration reverses the granulopoietic defect: one day of G-CSF administration increases circulating neutrophil levels to normal, and after 4 days of G-CSF administration, mutant bone marrows are morphologically indistinguishable from wild-type marrows   (MGI Ref ID J:20400)
  • immune system phenotype
  • abnormal neutrophil physiology
    • homozygotes exhibit impaired neutrophil mobilization in response to a single dose of G-CSF, with only 21% of wild-type circulating neutrophil levels at 3 hrs post-treatment   (MGI Ref ID J:20400)
  • decreased leukocyte cell number
    • homozygotes are viable, fertile, and developmentally normal with no overt signs of disease or significant changes in hemoglobin or platelet levels   (MGI Ref ID J:20400)
    • however, homozygotes exhibit leukopenia as a result of significant selective neutropenia   (MGI Ref ID J:20400)
    • some homozygotes exhibit reduced circulating lymphocyte levels; however, these changes are inconsistent   (MGI Ref ID J:20400)
    • decreased monocyte cell number
      • homozygotes display significantly reduced monocyte levels relative to wild-type mice   (MGI Ref ID J:20400)
    • decreased neutrophil cell number
      • homozygotes exhibit a severe chronic neutropenia, with blood neutrophil levels reduced to ~30% of wild-type levels   (MGI Ref ID J:20400)
      • however, no significant changes in circulating eosinophil numbers are observed   (MGI Ref ID J:20400)
  • impaired myelopoiesis
    • homozygotes show a 50% decrease in granulopoietic precursor cells in the bone marrow, with reduced levels of granulocyte, macrophage, and blast progenitor cells   (MGI Ref ID J:20400)
    • G-CSF administration reverses the granulopoietic defect: one day of G-CSF administration increases circulating neutrophil levels to normal, and after 4 days of G-CSF administration, mutant bone marrows are morphologically indistinguishable from wild-type marrows   (MGI Ref ID J:20400)
  • increased susceptibility to bacterial infection
    • homozygotes display a significantly impaired ability to control infection with Listeria monocytogenes, as shown by a 50% mortality rate at day 5 post-inoculation as well as diminished neutrophil and delayed monocyte increases in blood and reduced infection-driven granulopoiesis   (MGI Ref ID J:20400)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines

Research Tools
Immunology, Inflammation and Autoimmunity Research
      Macrophage Deficiency

Csf3tm1Ard related

Cancer Research
Growth Factors/Receptors/Cytokines

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Immunodeficiency

Research Tools
Immunology, Inflammation and Autoimmunity Research
      Macrophage Deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Csf3tm1Ard
Allele Name targeted mutation 1, Ashley R Dunn
Allele Type Targeted (Null/Knockout, Reporter)
Common Name(s) G-CSF-;
Mutation Made ByDr. Ashley Dunn,   Ludwig Institute for Cancer Research
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Csf3, colony stimulating factor 3 (granulocyte)
Chromosome 11
Gene Common Name(s) C17orf33; CSF3OS; Csfg; G-CSF; GCSF; MGI-IG; colony stimulating factor, granulocyte;
Molecular Note A 0.7kb genomic fragment containing exons 1-3 was replaced by a lacZ gene and neomycin resistance cassette. This mutation placed the lacZ gene under the control of the Csf3 promoter elements. An activity assay demonstrated that the functional protein was not detectable in cultured medium derived from organ cultures of various tissues of homozygous mice. [MGI Ref ID J:20400]

Genotyping

Genotyping Information

Genotyping Protocols

csf3tm1Ard 3' design, Separated PCR
Csf3tm1Ard, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Lieschke GJ; Grail D; Hodgson G; Metcalf D; Stanley E; Cheers C; Fowler KJ; Basu S; Zhan YF; Dunn AR. 1994. Mice lacking granulocyte colony-stimulating factor have chronic neutropenia, granulocyte and macrophage progenitor cell deficiency, and impaired neutrophil mobilization. Blood 84(6):1737-46. [PubMed: 7521686]  [MGI Ref ID J:20400]

Additional References

Csf3tm1Ard related

Basu S; Hodgson G; Katz M; Dunn AR. 2002. Evaluation of role of G-CSF in the production, survival, and release of neutrophils from bone marrow into circulation. Blood 100(3):854-61. [PubMed: 12130495]  [MGI Ref ID J:115546]

Basu S; Hodgson G; Zhang HH; Katz M; Quilici C; Dunn AR. 2000. 'Emergency' granulopoiesis in G-CSF-deficient mice in response to candida albicans infection Blood 95(12):3725-33. [PubMed: 10845903]  [MGI Ref ID J:63085]

Croker BA; Lewis RS; Babon JJ; Mintern JD; Jenne DE; Metcalf D; Zhang JG; Cengia LH; O'Donnell JA; Roberts AW. 2011. Neutrophils require SHP1 to regulate IL-1beta production and prevent inflammatory skin disease. J Immunol 186(2):1131-9. [PubMed: 21160041]  [MGI Ref ID J:168769]

Diederich K; Sevimli S; Dorr H; Kosters E; Hoppen M; Lewejohann L; Klocke R; Minnerup J; Knecht S; Nikol S; Sachser N; Schneider A; Gorji A; Sommer C; Schabitz WR. 2009. The role of granulocyte-colony stimulating factor (G-CSF) in the healthy brain: a characterization of G-CSF-deficient mice. J Neurosci 29(37):11572-81. [PubMed: 19759304]  [MGI Ref ID J:152761]

Dumortier A; Durham AD; Di Piazza M; Vauclair S; Koch U; Ferrand G; Ferrero I; Demehri S; Song LL; Farr AG; Leonard WJ; Kopan R; Miele L; Hohl D; Finke D; Radtke F. 2010. Atopic dermatitis-like disease and associated lethal myeloproliferative disorder arise from loss of notch signaling in the murine skin. PLoS One 5(2):e9258. [PubMed: 20174635]  [MGI Ref ID J:157987]

Eyles JL; Hickey MJ; Norman MU; Croker BA; Roberts AW; Drake SF; James WG; Metcalf D; Campbell IK; Wicks IP. 2008. A key role for G-CSF-induced neutrophil production and trafficking during inflammatory arthritis. Blood 112(13):5193-201. [PubMed: 18824600]  [MGI Ref ID J:144431]

Fang Y; Gong SJ; Xu YH; Hambly BD; Bao S. 2007. Impaired cutaneous wound healing in granulocyte/macrophage colony-stimulating factor knockout mice. Br J Dermatol 157(3):458-65. [PubMed: 17553038]  [MGI Ref ID J:147691]

He RL; Zhou J; Hanson CZ; Chen J; Cheng N; Ye RD. 2009. Serum amyloid A induces G-CSF expression and neutrophilia via Toll-like receptor 2. Blood 113(2):429-37. [PubMed: 18952897]  [MGI Ref ID J:144859]

Hermesh T; Moran TM; Jain D; Lopez CB. 2012. Granulocyte colony-stimulating factor protects mice during respiratory virus infections. PLoS One 7(5):e37334. [PubMed: 22615983]  [MGI Ref ID J:187229]

Jin DK; Shido K; Kopp HG; Petit I; Shmelkov SV; Young LM; Hooper AT; Amano H; Avecilla ST; Heissig B; Hattori K; Zhang F; Hicklin DJ; Wu Y; Zhu Z; Dunn A; Salari H; Werb Z; Hackett NR; Crystal RG; Lyden D; Rafii S. 2006. Cytokine-mediated deployment of SDF-1 induces revascularization through recruitment of CXCR4+ hemangiocytes. Nat Med 12(5):557-67. [PubMed: 16648859]  [MGI Ref ID J:109523]

Lawlor KE; Campbell IK; Metcalf D; O'Donnell K; van Nieuwenhuijze A; Roberts AW; Wicks IP. 2004. Critical role for granulocyte colony-stimulating factor in inflammatory arthritis. Proc Natl Acad Sci U S A 101(31):11398-403. [PubMed: 15272075]  [MGI Ref ID J:91780]

Mannering SI; Zhan Y; Gilbertson B; Lieschke GJ; Cheers C. 2000. T lymphocytes from granulocyte colony-stimulating factor-/- mice produce large quantities of interferon-gamma in a chronic infection model. Immunology 101(1):132-9. [PubMed: 11012764]  [MGI Ref ID J:110448]

Metcalf D; Robb L; Dunn AR; Mifsud S; Di Rago L. 1996. Role of granulocyte-macrophage colony-stimulating factor and granulocyte colony-stimulating factor in the development of an acute neutrophil inflammatory response in mice. Blood 88(10):3755-64. [PubMed: 8916939]  [MGI Ref ID J:36785]

Pflegerl P; Vesely P; Hantusch B; Schlederer M; Zenz R; Janig E; Steiner G; Meixner A; Petzelbauer P; Wolf P; Soleiman A; Egger G; Moriggl R; Kishimoto T; Wagner EF; Kenner L. 2009. Epidermal loss of JunB leads to a SLE phenotype due to hyper IL-6 signaling. Proc Natl Acad Sci U S A 106(48):20423-8. [PubMed: 19918056]  [MGI Ref ID J:155575]

Seymour JF; Lieschke GJ; Grail D; Quilici C; Hodgson G; Dunn AR. 1997. Mice lacking both granulocyte colony-stimulating factor (CSF) and granulocyte-macrophage CSF have impaired reproductive capacity, perturbed neonatal granulopoiesis, lung disease, amyloidosis, and reduced long-term survival. Blood 90(8):3037-49. [PubMed: 9376584]  [MGI Ref ID J:135551]

Tesio M; Golan K; Corso S; Giordano S; Schajnovitz A; Vagima Y; Shivtiel S; Kalinkovich A; Caione L; Gammaitoni L; Laurenti E; Buss EC; Shezen E; Itkin T; Kollet O; Petit I; Trumpp A; Christensen J; Aglietta M; Piacibello W; Lapidot T. 2011. Enhanced c-Met activity promotes G-CSF-induced mobilization of hematopoietic progenitor cells via ROS signaling. Blood 117(2):419-28. [PubMed: 20585044]  [MGI Ref ID J:168441]

Walker F; Zhang HH; Matthews V; Weinstock J; Nice EC; Ernst M; Rose-John S; Burgess AW. 2008. IL6/sIL6R complex contributes to emergency granulopoietic responses in G-CSF- and GM-CSF-deficient mice. Blood 111(8):3978-85. [PubMed: 18156493]  [MGI Ref ID J:134366]

Zhan Y; Lieschke GJ; Grail D; Dunn AR; Cheers C. 1998. Essential roles for granulocyte-macrophage colony-stimulating factor (GM-CSF) and G-CSF in the sustained hematopoietic response of Listeria monocytogenes-infected mice. Blood 91(3):863-9. [PubMed: 9446646]  [MGI Ref ID J:106590]

Zhang HH; Basu S; Wu F; Begley CG; Saris CJ; Dunn AR; Burgess AW; Walker F. 2007. Macrophage-colony stimulating factor is required for the production of neutrophil-promoting activity by mouse embryo fibroblasts deficient in G-CSF and GM-CSF. J Leukoc Biol 82(4):915-25. [PubMed: 17652450]  [MGI Ref ID J:125155]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThe Csf3-deficient strain is maintained by homozygous sibling matings. This strain should be housed under pathogen free conditions similar to the Prkdcscid/Prkdcscid mouse or any other immunodeficient strain. It is on a mixed C57BL/6 x 129/Ola genetic background.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   100903 B6129PF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.6)