Strain Name:

C57BL/6-Igk-Ctm1Cgn/J

Stock Number:

002400

Availability:

Repository-Cryopreserved

Use Restrictions Apply, see Terms of Use

Description

Strain Information

Type Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
GenerationF?+6p
 
Donating Investigator Klaus Rajewsky,   Harvard Medical School

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Igk-Ctm1Cgn targeted mutation are viable and fertile. The number of B cells expressing l chain is increased 2-fold in heterozygotes and 7-fold in homozygotes.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Igk-C
007595   B6;129-Igk-Ctm1(IGKC)Mnz/J
005018   NOD.Cg-Tg(Igk-C/Igk-V125)1Jwt/JwtJ
View Strains carrying other alleles of Igk-C     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Igk-Ctm1Cgn/Igk-C+

        involves: C57BL/6
  • immune system phenotype
  • increased mature B cell number (MGI Ref ID J:64287)
    • 2 fold increase of lambda-chain expressing B cells
  • hematopoietic system phenotype
  • increased mature B cell number (MGI Ref ID J:64287)
    • 2 fold increase of lambda-chain expressing B cells

Igk-Ctm1Cgn/Igk-Ctm1Cgn

        involves: C57BL/6
  • immune system phenotype
  • abnormal B cell differentiation (MGI Ref ID J:64287)
    • 50% decrease in recombination of the Jk-kappa and V-kappa gene segments in splenic B cells
    • increased immature B cell number (MGI Ref ID J:64287)
      • the percentage of immature B cells in bone marrow is double that of wild-type
  • decreased mature B cell number (MGI Ref ID J:64287)
    • 2.3 fold less B cells in the spleen
    • B cells expressing kappa chain completely absent in the spleen
    • the percentage of newly generated B cell in the bone marrow is half that of wild-type
  • increased mature B cell number (MGI Ref ID J:64287)
    • 7 fold increase of lambda-chain expressing B cells
  • spleen hypoplasia (MGI Ref ID J:64287)
    • 30% fewer nucleated cells found in the spleen
  • hematopoietic system phenotype
  • abnormal B cell differentiation (MGI Ref ID J:64287)
    • 50% decrease in recombination of the Jk-kappa and V-kappa gene segments in splenic B cells
    • increased immature B cell number (MGI Ref ID J:64287)
      • the percentage of immature B cells in bone marrow is double that of wild-type
  • decreased mature B cell number (MGI Ref ID J:64287)
    • 2.3 fold less B cells in the spleen
    • B cells expressing kappa chain completely absent in the spleen
    • the percentage of newly generated B cell in the bone marrow is half that of wild-type
  • increased mature B cell number (MGI Ref ID J:64287)
    • 7 fold increase of lambda-chain expressing B cells
  • spleen hypoplasia (MGI Ref ID J:64287)
    • 30% fewer nucleated cells found in the spleen
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Igk-C related

Immunology and Inflammation Research
Autoimmunity

Igk-Ctm1Cgn related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency

Genes & Alleles

Gene & Allele Information

Allele Symbol Igk-Ctm1Cgn
Allele Name targeted mutation 1, University of Cologne
Allele Type Targeted (knock-out)
Common Name(s) CkappaT; kappa-;
Mutation Made By Yong-Rui Zou,   University of Cologne
Strain of OriginC57BL/6
ES Cell Line NameBL/6-III
ES Cell Line StrainC57BL/6
Gene Symbol and Name Igk-C, immunoglobulin kappa chain, constant region
Chromosome 6
Gene Common Name(s) HCAK1; Igkc; Km; MGC111575; MGC62011; MGC72072; MGC88770; MGC88771; MGC88809;
Molecular Note A neomycin resistance cassette replaced the exon encoding the constant region of the immunoglobulin kappa chain, its splicing signal and polyadenylation site. [MGI Ref ID J:64287]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Zou YR; Takeda S; Rajewsky K. 1993. Gene targeting in the Ig kappa locus: efficient generation of lambda chain-expressing B cells, independent of gene rearrangements in Ig kappa. EMBO J 12(3):811-20. [PubMed: 8458339]  [MGI Ref ID J:64287]

Additional References

Igk-Ctm1Cgn related

Casellas R; Nussenzweig A; Wuerffel R; Pelanda R; Reichlin A; Suh H; Qin XF; Besmer E; Kenter A; Rajewsky K; Nussenzweig MC. 1998. Ku80 is required for immunoglobulin isotype switching. EMBO J 17(8):2404-11. [PubMed: 9545251]  [MGI Ref ID J:127088]

Mao C; Jiang L; Melo-Jorge M; Puthenveetil M; Zhang X; Carroll MC; Imanishi-Kari T. 2004. T cell-independent somatic hypermutation in murine B cells with an immature phenotype. Immunity 20(2):133-44. [PubMed: 14975236]  [MGI Ref ID J:89757]

Pape KA; Kouskoff V; Nemazee D; Tang HL; Cyster JG; Tze LE; Hippen KL; Behrens TW; Jenkins MK. 2003. Visualization of the genesis and fate of isotype-switched B cells during a primary immune response. J Exp Med 197(12):1677-87. [PubMed: 12796466]  [MGI Ref ID J:116662]

Pelanda R; Schaal S; Torres RM; Rajewsky K. 1996. A prematurely expressed Ig kappa transgene, but not a V kappa J kappa gene segment targeted into the Ig kappa locus, can rescue B cell development in lambda 5-deficient mice. Immunity 5(3):229-239. [PubMed: 8808678]  [MGI Ref ID J:35357]

Sonoda E; Pewzner-Jung Y; Schwers S; Taki S; Jung S; Eilat D; Rajewsky K. 1997. B cell development under the condition of allelic inclusion. Immunity 6(3):225-33. [PubMed: 9075923]  [MGI Ref ID J:79556]

Yamagami T; ten Boekel E; Schaniel C; Andersson J; Rolink A; Melchers F. 1999. Four of five RAG-expressing JCkappa-/- small pre-BII cells have no L chain gene rearrangements: detection by high-efficiency single cell PCR. Immunity 11(3):309-16. [PubMed: 10514009]  [MGI Ref ID J:57910]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


See Terms of Use


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

      Purchasing Information
      JAX® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:207-288-6470
fax:207-288-6655

JAX® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. THE LABORATORY EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.


(3.2)