Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F?+8pN1p+pN1   Donating Investigator John Roder,   University of Toronto

Appearance
white-bellied agouti
Related Genotype: A^w/A^w

Description
Mice homozygous for the Mag^tm1Rod targeted mutation are viable and fertile. Mag had previously been thought to be necessary for myelin formation. However in the homozygous mutant the degree of myelination and its compaction are normal. Finer motor coordination abilities are significantly affected in the homozygous mutant and they exhibit a subtle intention tremor. The organization of the periaxonal region is partially impaired with the periaxonal cytoplasmic collar frequently missing in optic nerve, cervical spinal cord, and ventral roots. Later in life, beginning at 6 months, oligodendrocytes degenerate. This strain may serve as a model for some aspects of multiple sclerosis. MAG also tranduces a signal to axons. Therefore, axons in the MAG-deficient mice are smaller in calliber due to the aberrant phosphorylation of neurofilaments. MAG has also been shown to be an inhibitor of nerve regeneration.

Development
The Mag-deficient strain was developed in the laboratory of Dr. John Roder at the Samuel Lunenfeld Research Institute in Toronto.

Control Information

	Control
	None Available
		This strain is a mixture of C57BL/6, 129 inbred strains and the CD1 random bred strain. Thus, there is no appropriate physiological control for this mixed genetic background.
Considerations for Choosing Controls

Related Strains

Strains carrying   Mag^tm1Rod allele

006865

B6.Cg-Magtm1Rod/J

View Strains carrying   Mag^tm1Rod     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Mag^tm1Rod/Mag^tm1Rod

        involves: 129S1/Sv * 129X1/SvJ

• nervous system phenotype
• abnormal axon morphology (MGI Ref ID J:18407)
  • optic nerve, cervical spinal cord, and ventral roots exhibit frequent loss of periaxonal cytoplasmic collar, periaxonal collar swelling, cytoplasm within myelin, and redundant myelin, however degree of myelination and its compaction are normal
  • the cytoplasmic leaflet of the periaxonal membrane is often fused with the inner compact myelin lamellae to form a dense line
  • when present, the cytoplasm of the periaxonal collar is usually disorganized
• behavior/neurological phenotype
• abnormal grooming behavior (MGI Ref ID J:18407)
  • less frequent exploratory sniffing and grooming on a bar-cross apparatus
• hypoactivity (MGI Ref ID J:18407)
  • show decreased locomotor activity on a bar-cross apparatus
• impaired coordination (MGI Ref ID J:18407)
  • slower to transverse a narrow challenge bar
• tremors (MGI Ref ID J:18407)
  • many homozygotes exhibit a mild, transient, trunk tremor on a bar-cross apparatus

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Mag^tm1Rod related

Neurobiology Research
Myelination Defects
Tremor Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Magtm1Rod
Allele Name		targeted mutation 1, John Roder
Allele Type		Targeted (knock-out)
Mutation Made By		John Roder,   University of Toronto
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Mag, myelin-associated glycoprotein
Chromosome		7
Gene Common Name(s)		GMA; Gma; S-MAG; SIGLEC-4A; SIGLEC4A;
Molecular Note		A neomycin resistance cassette was inserted into exon 5 of the gene. Mutant mice showed a complete lack of mRNA and protein in the central nervous system for the targeted gene. Nerves from the peripheral nervous system also lacked protein for the targeted gene. [MGI Ref ID J:18407]

Genotyping

Genotyping Information

Genotyping Protocols

Mag^tm1Rod, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Li C; Tropak MB; Gerlai R; Clapoff S; Abramow-Newerly W; Trapp B; Peterson A; Roder J. 1994. Myelination in the absence of myelin-associated glycoprotein. Nature 369(6483):747-50. [PubMed: 7516497]  [MGI Ref ID J:18407]

Additional References

Li C; Trapp B; Ludwin S; Peterson A; Roder J. 1998. Myelin associated glycoprotein modulates glia-axon contact in vivo. J Neurosci Res 51(2):210-7. [PubMed: 9469574]  [MGI Ref ID J:45616]

Montag D; Giese KP; Bartsch U; Martini R; Lang Y; Bluthmann H; Karthigasan J; Kirschner DA; Wintergerst ES; Nave KA; Zielasek J; Toyka KV; Lipp HP; Schachner M. 1994. Mice deficient for the myelin-associated glycoprotein show subtle abnormalities in myelin. Neuron 13(1):229-46. [PubMed: 7519026]  [MGI Ref ID J:19475]

Vabnick I; Messing A; Chiu SY; Levinson SR; Schachner M; Roder J; Li C; Novakovic S; Shrager P. 1997. Sodium channel distribution in axons of hypomyelinated and MAG null mutant mice. J Neurosci Res 50(2):321-36. [PubMed: 9373041]  [MGI Ref ID J:44044]

Mag^tm1Rod related

Cai Z; Sutton-Smith P; Swift J; Cash K; Finnie J; Turnley A; Thompson PD; Blumbergs PC. 2002. Tomacula in MAG-deficient mice. J Peripher Nerv Syst 7(3):181-9. [PubMed: 12365566]  [MGI Ref ID J:103883]

Fry EJ; Ho C; David S. 2007. A role for Nogo receptor in macrophage clearance from injured peripheral nerve. Neuron 53(5):649-62. [PubMed: 17329206]  [MGI Ref ID J:122959]

Haney CA; Sahenk Z; Li C; Lemmon VP; Roder J; Trapp BD. 1999. Heterophilic binding of L1 on unmyelinated sensory axons mediates Schwann cell adhesion and is required for axonal survival. J Cell Biol 146(5):1173-84. [PubMed: 10477768]  [MGI Ref ID J:57603]

Kumar S; Yin X; Trapp BD; Paulaitis ME; Hoh JH. 2002. Role of long-range repulsive forces in organizing axonal neurofilament distributions: evidence from mice deficient in myelin-associated glycoprotein. J Neurosci Res 68(6):681-90. [PubMed: 12111829]  [MGI Ref ID J:104964]

Li C; Trapp B; Ludwin S; Peterson A; Roder J. 1998. Myelin associated glycoprotein modulates glia-axon contact in vivo. J Neurosci Res 51(2):210-7. [PubMed: 9469574]  [MGI Ref ID J:45616]

Marcus J; Dupree JL; Popko B. 2002. Myelin-associated glycoprotein and myelin galactolipids stabilize developing axo-glial interactions. J Cell Biol 156(3):567-77. [PubMed: 11827985]  [MGI Ref ID J:77227]

Pan B; Fromholt SE; Hess EJ; Crawford TO; Griffin JW; Sheikh KA; Schnaar RL. 2005. Myelin-associated glycoprotein and complementary axonal ligands, gangliosides, mediate axon stability in the CNS and PNS: neuropathology and behavioral deficits in single- and double-null mice. Exp Neurol 195(1):208-17. [PubMed: 15953602]  [MGI Ref ID J:100940]

Uschkureit T; Sporkel O; Stracke J; Bussow H; Stoffel W. 2000. Early onset of axonal degeneration in double (plp-/-mag-/-) and hypomyelinosis in triple (plp-/-mbp-/-mag-/-) mutant mice. J Neurosci 20(14):5225-33. [PubMed: 10884306]  [MGI Ref ID J:63480]

Vabnick I; Messing A; Chiu SY; Levinson SR; Schachner M; Roder J; Li C; Novakovic S; Shrager P. 1997. Sodium channel distribution in axons of hypomyelinated and MAG null mutant mice. J Neurosci Res 50(2):321-36. [PubMed: 9373041]  [MGI Ref ID J:44044]

Weiss MD; Luciano CA; Quarles RH. 2001. Nerve conduction abnormalities in aging mice deficient for myelin-associated glycoprotein. Muscle Nerve 24(10):1380-7. [PubMed: 11562920]  [MGI Ref ID J:116346]

Yin X; Kidd GJ; Nave KA; Trapp BD. 2008. P0 protein is required for and can induce formation of schmidt-lantermann incisures in myelin internodes. J Neurosci 28(28):7068-73. [PubMed: 18614675]  [MGI Ref ID J:137961]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	This strain is maintained by homozygous sibling matings. Expected coat color from breeding:White Bellied Agouti

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	None Available
		This strain is a mixture of C57BL/6, 129 inbred strains and the CD1 random bred strain. Thus, there is no appropriate physiological control for this mixed genetic background.
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Terms of Use

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General Terms and Conditions

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Contact information

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phone:	207-288-6470
fax:	207-288-6655

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