Strain Name:

B6.129P2-Ncam1tm1Cgn/J

Stock Number:

002405

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Availability:

Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129P2-Ncamtm1Cgn    (Changed: 15-DEC-04 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain 129P2 via E14-1 ES cell line
 
Donating Investigator Klaus Rajewsky,   Max-Delbruck-Ctr. for Molecular Medicine

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Ncam1tm1Cgn targeted mutation show a 10% reduction in overall brain weight and 36% reduction in olfactory bulb size. Motor end plates were 14% smaller in NCAM-deficient mice compared to controls, and the formation of junctional folds was delayed. They also show deficits in spatial learning and in the amount of protein-bound alpha-(2,8)-linked polysialic acid. Both homozygous and heterozygous mutant mice are reportedly more aggressive than wildtype controls.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Ncam1tm1Cgn/Ncam1tm1Cgn

        involves: C57BL/6J
  • behavior/neurological phenotype
  • abnormal circadian period
    • in 50% of mutant in DD conditions aberrant activity rhythms make it impossible to determine a period   (MGI Ref ID J:96708)
    • these mutants also had a longer active period in DD conditions   (MGI Ref ID J:96708)
    • shortened circadian period
      • the circadian period in DD conditions is significantly shorter in 50% of mutants, 23.7 hours compared to 23.98 hours in wild-type littermates   (MGI Ref ID J:96708)
  • abnormal circadian phase
    • daily onset and offset of activity are irregular or lost   (MGI Ref ID J:96708)

Ncam1tm1Cgn/Ncam1tm1Cgn

        B6.129P2-Ncam1tm1Cgn
  • nervous system phenotype
  • abnormal CNS glial cell morphology
    • the number of GFAP+ cells is increased along the rostral migratory stream but these cells are not within the stream nor directed to the olfactory bulb   (MGI Ref ID J:126687)
  • abnormal neuron differentiation
    • more neurospheres differentiate into GFAP+ cells when exposed to PDGF (44.1+/-7.5% compared to 33.0+/-4.5% of wild-type neurospheres)   (MGI Ref ID J:126687)
  • cellular phenotype
  • abnormal neuron differentiation
    • more neurospheres differentiate into GFAP+ cells when exposed to PDGF (44.1+/-7.5% compared to 33.0+/-4.5% of wild-type neurospheres)   (MGI Ref ID J:126687)

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Ncam1tm1Cgn/Ncam1tm1Cgn

        involves: 129P2/OlaHsd
  • behavior/neurological phenotype
  • abnormal spatial learning
    • spatial learning impairment demonstrated in the Morris water maze test   (MGI Ref ID J:16590)
  • nervous system phenotype
  • decreased brain size   (MGI Ref ID J:16590)
  • small olfactory bulb   (MGI Ref ID J:16590)

Ncam1tm1Cgn/Ncam1tm1Cgn

        Background Not Specified
  • growth/size/body phenotype
  • decreased body weight   (MGI Ref ID J:165965)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Ncam1tm1Cgn related

Cancer Research
Defects in Cell Adhesion Molecules
Growth Factors/Receptors/Cytokines

Developmental Biology Research
Neurodevelopmental Defects

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Growth Factors/Receptors/Cytokines

Neurobiology Research
Neurodevelopmental Defects
Neurotrophic Factor Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ncam1tm1Cgn
Allele Name targeted mutation 1, University of Cologne
Allele Type Targeted (knock-out)
Common Name(s) N-CAM-; N-CAM-1-; NCAM-; Ncam-;
Mutation Made By Harold Cremer,   Developmental Biology Inst. of Marseille
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14.1
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Ncam1, neural cell adhesion molecule 1
Chromosome 9
Gene Common Name(s) CD56; E-NCAM; MSK39; N-CAM; N-CAM-1; NCAM; NCAM-1; NCAM-120; NCAM-140; NCAM-180; NCAM-C; NCAMC;
Molecular Note A neomycin resistance cassette replaced most of exons 3 and 4 and the intervening intron. Ribonuclease protection assays did not detect wild-type or read-through transcripts or alternative or cryptic splicing in brain tissue from homozygous mutant mice. Western blot analysis of brain tissue using a monoclonal antibody or a polyclonal rabbit serum did not detect protein in homozygous mutant mice. Similarly, immunostaining did not detect protein in mutant olfactory bulb. [MGI Ref ID J:16590]

Genotyping

Genotyping Information

Genotyping Protocols

Ncamtm1Cgn, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Cremer H; Lange R; Christoph A; Plomann M; Vopper G; Roes J; Brown R; Baldwin S; Kraemer P; Scheff S; Barthels D; Rajewsky K; Wille W. 1994. Inactivation of the N-CAM gene in mice results in size reduction of the olfactory bulb and deficits in spatial learning. Nature 367(6462):455-9. [PubMed: 8107803]  [MGI Ref ID J:16590]

Additional References

Rolf B; Bastmeyer M; Schachner M; Bartsch U. 2002. Pathfinding errors of corticospinal axons in neural cell adhesion molecule-deficient mice. J Neurosci 22(19):8357-62. [PubMed: 12351709]  [MGI Ref ID J:79210]

Stork O; Welzl H; Cremer H; Schachner M. 1997. Increased intermale aggression and neuroendocrine response in mice deficient for the neural cell adhesion molecule (NCAM). Eur J Neurosci 9(6):1117-25. [PubMed: 9215693]  [MGI Ref ID J:43343]

Ncam1tm1Cgn related

Angata K; Huckaby V; Ranscht B; Terskikh A; Marth JD; Fukuda M. 2007. Polysialic acid-directed migration and differentiation of neural precursors are essential for mouse brain development. Mol Cell Biol 27(19):6659-68. [PubMed: 17682066]  [MGI Ref ID J:126687]

Aonurm-Helm A; Berezin V; Bock E; Zharkovsky A. 2010. NCAM-mimetic, FGL peptide, restores disrupted fibroblast growth factor receptor (FGFR) phosphorylation and FGFR mediated signaling in neural cell adhesion molecule (NCAM)-deficient mice. Brain Res 1309:1-8. [PubMed: 19909731]  [MGI Ref ID J:158544]

Aonurm-Helm A; Jurgenson M; Zharkovsky T; Sonn K; Berezin V; Bock E; Zharkovsky A. 2008. Depression-like behaviour in neural cell adhesion molecule (NCAM)-deficient mice and its reversal by an NCAM-derived peptide, FGL. Eur J Neurosci 28(8):1618-28. [PubMed: 18973581]  [MGI Ref ID J:143245]

Aonurm-Helm A; Zharkovsky T; Jurgenson M; Kalda A; Zharkovsky A. 2008. Dysregulated CREB signaling pathway in the brain of neural cell adhesion molecule (NCAM)-deficient mice. Brain Res 1243:104-12. [PubMed: 18817764]  [MGI Ref ID J:147626]

Bodrikov V; Sytnyk V; Leshchyns'ka I; den Hertog J; Schachner M. 2008. NCAM induces CaMKIIalpha-mediated RPTPalpha phosphorylation to enhance its catalytic activity and neurite outgrowth. J Cell Biol 182(6):1185-200. [PubMed: 18809727]  [MGI Ref ID J:142818]

Boutin C; Schmitz B; Cremer H; Diestel S. 2009. NCAM expression induces neurogenesis in vivo. Eur J Neurosci 30(7):1209-18. [PubMed: 19788570]  [MGI Ref ID J:155440]

Brennaman LH; Zhang X; Guan H; Triplett JW; Brown A; Demyanenko GP; Manis PB; Landmesser L; Maness PF. 2013. Polysialylated NCAM and ephrinA/EphA regulate synaptic development of GABAergic interneurons in prefrontal cortex. Cereb Cortex 23(1):162-77. [PubMed: 22275477]  [MGI Ref ID J:204427]

Carenini S; Montag D; Cremer H; Schachner M; Martini R. 1997. Absence of the myelin-associated glycoprotein (MAG) and the neural cell adhesion molecule (N-CAM) interferes with the maintenance, but not with the formation of peripheral myelin. Cell Tissue Res 287(1):3-9. [PubMed: 9011400]  [MGI Ref ID J:78646]

Carenini S; Montag D; Schachner M; Martini R. 1999. Subtle roles of neural cell adhesion molecule and myelin-associated glycoprotein during Schwann cell spiralling in P0-deficient mice. Glia 27(3):203-12. [PubMed: 10457367]  [MGI Ref ID J:113080]

Cassens C; Kleene R; Xiao MF; Friedrich C; Dityateva G; Schafer-Nielsen C; Schachner M. 2010. Binding of the receptor tyrosine kinase TrkB to the neural cell adhesion molecule (NCAM) regulates phosphorylation of NCAM and NCAM-dependent neurite outgrowth. J Biol Chem 285(37):28959-67. [PubMed: 20605779]  [MGI Ref ID J:166303]

Chan SA; Polo-Parada L; Landmesser LT; Smith C. 2005. Adrenal chromaffin cells exhibit impaired granule trafficking in NCAM knockout mice. J Neurophysiol 94(2):1037-47. [PubMed: 15800072]  [MGI Ref ID J:114291]

Chazal G; Durbec P; Jankovski A; Rougon G; Cremer H. 2000. Consequences of neural cell adhesion molecule deficiency on cell migration in the rostral migratory stream of the mouse. J Neurosci 20(4):1446-57. [PubMed: 10662835]  [MGI Ref ID J:60288]

Chipman PH; Franz CK; Nelson A; Schachner M; Rafuse VF. 2010. Neural cell adhesion molecule is required for stability of reinnervated neuromuscular junctions. Eur J Neurosci 31(2):238-49. [PubMed: 20074227]  [MGI Ref ID J:158382]

Cremer H; Chazal G; Carleton A; Goridis C; Vincent JD; Lledo PM. 1998. Long-term but not short-term plasticity at mossy fiber synapses is impaired in neural cell adhesion molecule-deficient mice. Proc Natl Acad Sci U S A 95(22):13242-7. [PubMed: 9789073]  [MGI Ref ID J:50572]

Cremer H; Chazal G; Goridis C; Represa A. 1997. NCAM is essential for axonal growth and fasciculation in the hippocampus. Mol Cell Neurosci 8(5):323-35. [PubMed: 9073395]  [MGI Ref ID J:40113]

Crnic I; Strittmatter K; Cavallaro U; Kopfstein L; Jussila L; Alitalo K; Christofori G. 2004. Loss of neural cell adhesion molecule induces tumor metastasis by up-regulating lymphangiogenesis. Cancer Res 64(23):8630-8. [PubMed: 15574770]  [MGI Ref ID J:94451]

Decker L; Durbec P; Rougon G; Evercooren AB. 2002. Loss of polysialic residues accelerates CNS neural precursor differentiation in pathological conditions. Mol Cell Neurosci 19(2):225-38. [PubMed: 11860275]  [MGI Ref ID J:75427]

Delling M; Wischmeyer E; Dityatev A; Sytnyk V; Veh RW; Karschin A; Schachner M. 2002. The neural cell adhesion molecule regulates cell-surface delivery of G-protein-activated inwardly rectifying potassium channels via lipid rafts. J Neurosci 22(16):7154-64. [PubMed: 12177211]  [MGI Ref ID J:128160]

Diestel S; Schaefer D; Cremer H; Schmitz B. 2007. NCAM is ubiquitylated, endocytosed and recycled in neurons. J Cell Sci 120(Pt 22):4035-49. [PubMed: 17971410]  [MGI Ref ID J:130267]

Esni F; Taljedal IB; Perl AK; Cremer H; Christofori G; Semb H. 1999. Neural cell adhesion molecule (N-CAM) is required for cell type segregation and normal ultrastructure in pancreatic islets. J Cell Biol 144(2):325-37. [PubMed: 9922458]  [MGI Ref ID J:52451]

Euteneuer S; Yang KH; Chavez E; Leichtle A; Loers G; Olshansky A; Pak K; Schachner M; Ryan AF. 2013. Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM). Mol Cell Neurosci 54:30-43. [PubMed: 23262364]  [MGI Ref ID J:203649]

Franz CK; Rutishauser U; Rafuse VF. 2005. Polysialylated neural cell adhesion molecule is necessary for selective targeting of regenerating motor neurons. J Neurosci 25(8):2081-91. [PubMed: 15728848]  [MGI Ref ID J:97409]

Gascon E; Vutskits L; Jenny B; Durbec P; Kiss JZ. 2007. PSA-NCAM in postnatally generated immature neurons of the olfactory bulb: a crucial role in regulating p75 expression and cell survival. Development 134(6):1181-90. [PubMed: 17301083]  [MGI Ref ID J:118866]

Gomez-Climent MA; Guirado R; Castillo-Gomez E; Varea E; Gutierrez-Mecinas M; Gilabert-Juan J; Garcia-Mompo C; Vidueira S; Sanchez-Mataredona D; Hernandez S; Blasco-Ibanez JM; Crespo C; Rutishauser U; Schachner M; Nacher J. 2011. The polysialylated form of the neural cell adhesion molecule (PSA-NCAM) is expressed in a subpopulation of mature cortical interneurons characterized by reduced structural features and connectivity. Cereb Cortex 21(5):1028-41. [PubMed: 20843898]  [MGI Ref ID J:183825]

Gubkina O; Cremer H; Rougon G. 2001. Mutation in the neural cell adhesion molecule interferes with the differentiation of anterior pituitary secretory cells. Neuroendocrinology 74(5):335-46. [PubMed: 11694765]  [MGI Ref ID J:73315]

Jurgenson M; Aonurm-Helm A; Zharkovsky A. 2012. Partial reduction in neural cell adhesion molecule (NCAM) in heterozygous mice induces depression-related behaviour without cognitive impairment. Brain Res 1447:106-18. [PubMed: 22361116]  [MGI Ref ID J:181837]

Kleene R; Cassens C; Bahring R; Theis T; Xiao MF; Dityatev A; Schafer-Nielsen C; Doring F; Wischmeyer E; Schachner M. 2010. Functional consequences of the interactions among the neural cell adhesion molecule NCAM, the receptor tyrosine kinase TrkB, and the inwardly rectifying K+ channel KIR3.3. J Biol Chem 285(37):28968-79. [PubMed: 20610389]  [MGI Ref ID J:166300]

Kochlamazashvili G; Bukalo O; Senkov O; Salmen B; Gerardy-Schahn R; Engel AK; Schachner M; Dityatev A. 2012. Restoration of Synaptic Plasticity and Learning in Young and Aged NCAM-Deficient Mice by Enhancing Neurotransmission Mediated by GluN2A-Containing NMDA Receptors. J Neurosci 32(7):2263-2275. [PubMed: 22396402]  [MGI Ref ID J:181613]

Ledda F; Paratcha G; Sandoval-Guzman T; Ibanez CF. 2007. GDNF and GFRalpha1 promote formation of neuronal synapses by ligand-induced cell adhesion. Nat Neurosci 10(3):293-300. [PubMed: 17310246]  [MGI Ref ID J:120732]

Li S; Leshchyns'ka I; Chernyshova Y; Schachner M; Sytnyk V. 2013. The neural cell adhesion molecule (NCAM) associates with and signals through p21-activated kinase 1 (Pak1). J Neurosci 33(2):790-803. [PubMed: 23303955]  [MGI Ref ID J:193907]

Loers G; Aboul-Enein F; Bartsch U; Lassmann H; Schachner M. 2004. Comparison of myelin, axon, lipid, and immunopathology in the central nervous system of differentially myelin-compromised mutant mice: a morphological and biochemical study. Mol Cell Neurosci 27(2):175-89. [PubMed: 15485773]  [MGI Ref ID J:93204]

Meier ID; Bernreuther C; Tilling T; Neidhardt J; Wong YW; Schulze C; Streichert T; Schachner M. 2010. Short DNA sequences inserted for gene targeting can accidentally interfere with off-target gene expression. FASEB J 24(6):1714-24. [PubMed: 20110269]  [MGI Ref ID J:162048]

Montag-Sallaz M; Montag D; Schachner M. 2003. Altered processing of novel information in N-CAM-deficient mice. Neuroreport 14(10):1343-6. [PubMed: 12876470]  [MGI Ref ID J:89768]

Moscoso LM; Cremer H; Sanes JR. 1998. Organization and reorganization of neuromuscular junctions in mice lacking neural cell adhesion molecule, tenascin-C, or fibroblast growth factor-5. J Neurosci 18(4):1465-77. [PubMed: 9454855]  [MGI Ref ID J:45762]

Mouse Genome Informatics and the Europhenome Mouse Phenotyping Resource. 2010. Obtaining and Loading Phenotype Annotations from Europhenome Database Release :.  [MGI Ref ID J:165965]

Murphy JA; Franklin TB; Rafuse VF; Clarke DB. 2007. The neural cell adhesion molecule is necessary for normal adult retinal ganglion cell number and survival. Mol Cell Neurosci 36(2):280-92. [PubMed: 17716914]  [MGI Ref ID J:126751]

Murphy JA; Hartwick AT; Rutishauser U; Clarke DB. 2009. Endogenous polysialylated neural cell adhesion molecule enhances the survival of retinal ganglion cells. Invest Ophthalmol Vis Sci 50(2):861-9. [PubMed: 18757519]  [MGI Ref ID J:146691]

Ng KL; Li JD; Cheng MY; Leslie FM; Lee AG; Zhou QY. 2005. Dependence of olfactory bulb neurogenesis on prokineticin 2 signaling. Science 308(5730):1923-7. [PubMed: 15976302]  [MGI Ref ID J:99245]

Olofsson CS; Hakansson J; Salehi A; Bengtsson M; Galvanovskis J; Partridge C; SorhedeWinzell M; Xian X; Eliasson L; Lundquist I; Semb H; Rorsman P. 2009. Impaired insulin exocytosis in neural cell adhesion molecule-/- mice due to defective reorganization of the submembrane F-actin network. Endocrinology 150(7):3067-75. [PubMed: 19213846]  [MGI Ref ID J:151804]

Paratcha G; Ibanez CF; Ledda F. 2006. GDNF is a chemoattractant factor for neuronal precursor cells in the rostral migratory stream. Mol Cell Neurosci 31(3):505-14. [PubMed: 16380265]  [MGI Ref ID J:106863]

Perl AK; Dahl U; Wilgenbus P; Cremer H; Semb H; Christofori G. 1999. Reduced expression of neural cell adhesion molecule induces metastatic dissemination of pancreatic beta tumor cells. Nat Med 5(3):286-91. [PubMed: 10086383]  [MGI Ref ID J:53305]

Plappert CF; Schachner M; Pilz PK. 2006. Neural cell adhesion molecule (NCAM-/-) null mice show impaired sensitization of the startle response. Genes Brain Behav 5(1):46-52. [PubMed: 16436188]  [MGI Ref ID J:118083]

Plappert CF; Schachner M; Pilz PK. 2005. Neural cell adhesion molecule-null mice are not deficient in prepulse inhibition of the startle response. Neuroreport 16(9):1009-12. [PubMed: 15931078]  [MGI Ref ID J:103566]

Polo-Parada L; Bose CM; Landmesser LT. 2001. Alterations in Transmission, Vesicle Dynamics, and Transmitter Release Machinery at NCAM-Deficient Neuromuscular Junctions. Neuron 32(5):815-28. [PubMed: 11738028]  [MGI Ref ID J:73253]

Polo-Parada L; Bose CM; Plattner F; Landmesser LT. 2004. Distinct roles of different neural cell adhesion molecule (NCAM) isoforms in synaptic maturation revealed by analysis of NCAM 180 kDa isoform-deficient mice. J Neurosci 24(8):1852-64. [PubMed: 14985425]  [MGI Ref ID J:96999]

Potschka H; Pekcec A; Weinhold B; Gerardy-Schahn R. 2008. Deficiency of neural cell adhesion molecule or its polysialylation modulates pharmacological effects of the AMPA receptor antagonist NBQX. Neuroscience 152(4):1093-8. [PubMed: 18329813]  [MGI Ref ID J:135413]

Pozas E; Ibanez CF. 2005. GDNF and GFRalpha1 promote differentiation and tangential migration of cortical GABAergic neurons. Neuron 45(5):701-13. [PubMed: 15748846]  [MGI Ref ID J:99763]

Rafuse VF; Polo-Parada L; Landmesser LT. 2000. Structural and functional alterations of neuromuscular junctions in NCAM-deficient mice J Neurosci 20(17):6529-39. [PubMed: 10964958]  [MGI Ref ID J:64212]

Rolf B; Bastmeyer M; Schachner M; Bartsch U. 2002. Pathfinding errors of corticospinal axons in neural cell adhesion molecule-deficient mice. J Neurosci 22(19):8357-62. [PubMed: 12351709]  [MGI Ref ID J:79210]

Rollenhagen M; Kuckuck S; Ulm C; Hartmann M; Galuska SP; Geyer R; Geyer H; Muhlenhoff M. 2012. Polysialylation of the synaptic cell adhesion molecule 1 (SynCAM 1) depends exclusively on the polysialyltransferase ST8SiaII in vivo. J Biol Chem 287(42):35170-80. [PubMed: 22908220]  [MGI Ref ID J:191893]

Schellinck HM; Arnold A; Rafuse VF. 2004. Neural cell adhesion molecule (NCAM) null mice do not show a deficit in odour discrimination learning. Behav Brain Res 152(2):327-34. [PubMed: 15196800]  [MGI Ref ID J:95942]

Schiff M; Rockle I; Burkhardt H; Weinhold B; Hildebrandt H. 2011. Thalamocortical pathfinding defects precede degeneration of the reticular thalamic nucleus in polysialic Acid-deficient mice. J Neurosci 31(4):1302-12. [PubMed: 21273415]  [MGI Ref ID J:168547]

Schiff M; Weinhold B; Grothe C; Hildebrandt H. 2009. NCAM and polysialyltransferase profiles match dopaminergic marker gene expression but polysialic acid is dispensable for development of the midbrain dopamine system. J Neurochem 110(5):1661-73. [PubMed: 19619134]  [MGI Ref ID J:152222]

Senkov O; Sun M; Weinhold B; Gerardy-Schahn R; Schachner M; Dityatev A. 2006. Polysialylated neural cell adhesion molecule is involved in induction of long-term potentiation and memory acquisition and consolidation in a fear-conditioning paradigm. J Neurosci 26(42):10888-109898. [PubMed: 17050727]  [MGI Ref ID J:113438]

Shen H; Watanabe M; Tomasiewicz H; Glass JD. 2001. Genetic deletions of NCAM and PSA impair circadian function in the mouse. Physiol Behav 73(1-2):185-93. [PubMed: 11399310]  [MGI Ref ID J:96708]

Shetty A; Sytnyk V; Leshchyns'ka I; Puchkov D; Haucke V; Schachner M. 2013. The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms. J Neurosci 33(42):16828-45. [PubMed: 24133283]  [MGI Ref ID J:204686]

Shi Y; Xia YY; Wang L; Liu R; Khoo KS; Feng ZW. 2012. Neural cell adhesion molecule modulates mesenchymal stromal cell migration via activation of MAPK/ERK signaling. Exp Cell Res 318(17):2257-67. [PubMed: 22683856]  [MGI Ref ID J:187071]

Singh U; Sun T; Shi W; Schulz R; Nuber UA; Varanou A; Hemberger MC; Elliott RW; Ohta H; Wakayama T; Fundele R. 2005. Expression and functional analysis of genes deregulated in mouse placental overgrowth models: Car2 and Ncam1. Dev Dyn 234(4):1034-45. [PubMed: 16247769]  [MGI Ref ID J:102850]

Singhal N; Xu R; Martin PT. 2012. Distinct contributions of Galgt1 and Galgt2 to carbohydrate expression and function at the mouse neuromuscular junction. Mol Cell Neurosci 51(3-4):112-26. [PubMed: 22982027]  [MGI Ref ID J:203675]

Stoenica L; Senkov O; Gerardy-Schahn R; Weinhold B; Schachner M; Dityatev A. 2006. In vivo synaptic plasticity in the dentate gyrus of mice deficient in the neural cell adhesion molecule NCAM or its polysialic acid. Eur J Neurosci 23(9):2255-64. [PubMed: 16706834]  [MGI Ref ID J:108923]

Stork O; Welzl H; Wotjak CT; Hoyer D; Delling M; Cremer H; Schachner M. 1999. Anxiety and increased 5-HT1A receptor response in NCAM null mutant mice. J Neurobiol 40(3):343-55. [PubMed: 10440734]  [MGI Ref ID J:57426]

Tereshchenko Y; Morellini F; Dityatev A; Schachner M; Irintchev A. 2011. Neural cell adhesion molecule ablation in mice causes hippocampal dysplasia and loss of septal cholinergic neurons. J Comp Neurol 519(12):2475-92. [PubMed: 21456025]  [MGI Ref ID J:187347]

Theodosis DT; Schachner M; Neumann ID. 2004. Oxytocin neuron activation in NCAM-deficient mice: anatomical and functional consequences. Eur J Neurosci 20(12):3270-80. [PubMed: 15610159]  [MGI Ref ID J:101271]

Troncoso E; Muller D; Korodi K; Steimer T; Welker E; Kiss JZ. 2004. Recovery of evoked potentials, metabolic activity and behavior in a mouse model of somatosensory cortex lesion: role of the neural cell adhesion molecule (NCAM). Cereb Cortex 14(3):332-41. [PubMed: 14754871]  [MGI Ref ID J:106318]

Weinhold B; Seidenfaden R; Rockle I; Muhlenhoff M; Schertzinger F; Conzelmann S; Marth JD; Gerardy-Schahn R; Hildebrandt H. 2005. Genetic ablation of polysialic acid causes severe neurodevelopmental defects rescued by deletion of the neural cell adhesion molecule. J Biol Chem 280(52):42971-7. [PubMed: 16267048]  [MGI Ref ID J:105899]

Westphal D; Sytnyk V; Schachner M; Leshchyns'ka I. 2010. Clustering of the neural cell adhesion molecule (NCAM) at the neuronal cell surface induces caspase-8- and -3-dependent changes of the spectrin meshwork required for NCAM-mediated neurite outgrowth. J Biol Chem 285(53):42046-57. [PubMed: 20961848]  [MGI Ref ID J:167577]

Xian X; Hakansson J; Stahlberg A; Lindblom P; Betsholtz C; Gerhardt H; Semb H. 2006. Pericytes limit tumor cell metastasis. J Clin Invest 116(3):642-51. [PubMed: 16470244]  [MGI Ref ID J:106508]

Xiao MF; Xu JC; Tereshchenko Y; Novak D; Schachner M; Kleene R. 2009. Neural cell adhesion molecule modulates dopaminergic signaling and behavior by regulating dopamine D2 receptor internalization. J Neurosci 29(47):14752-63. [PubMed: 19940170]  [MGI Ref ID J:158473]

Zumsteg A; Strittmatter K; Klewe-Nebenius D; Antoniadis H; Christofori G. 2010. A bioluminescent mouse model of pancreatic {beta}-cell carcinogenesis. Carcinogenesis 31(8):1465-74. [PubMed: 20530553]  [MGI Ref ID J:162648]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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