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Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation F?+F48 (16-NOV-08) Donating Investigator Stephen Hedrick, University of California at San Diego Appearance
albino
Related Genotype: p Tyrc/p TyrcDescription
Mice express a T-cell receptor (V alpha 11.1 / V beta 3) specific for a carboxy-terminal fragment of pigeon cytochrome c and the Ek (class II MHC) molecule. Mice have been backcrossed a limited number of generations to C57BL/6 and fixed for H2b. Since C57BL/6 lacks the Ek allele, this mouse must be crossed to a strain that expresses Ek to study the interaction of the transgenic T-cell receptor with the pigeon cytochrome c antigen. When crossed with a strain carrying Ek, F1 progeny express an abnormally high percentage of class II MHC restricted mature CD4+CD8- cells. The peripheral T-cell population is almost exclusively CD4+. The lack of expression of Ek in the transgenic line allows it to serve as a universal donor for crossing the transgene onto other strains expressing Ek .Development
This class II MHC-restricted transgenic strain was developed in the laboratory of Dr. Stephen Hedrick at the University of California, San Diego.
Control Information
| Control | ||
|---|---|---|
| 100012 B6SJLF1/J | (approximate) | |
| 000664 C57BL/6J | (approximate) | |
| 000686 SJL/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(TcrAND)53Hed allele
002761 B10.Cg-Tg(TcrAND)53Hed/J View Strains carrying Tg(TcrAND)53Hed (1 strain)
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(TcrAND)53Hed/?
involves: A/WySnSg * C57BL/6 * C57BL/10SnSg * SJL
- immune system phenotype
- abnormal T cell differentiation (MGI Ref ID J:85777)
- 75% of thymocytes express high levels of CD3 compared to 18% of wild-type thymocytes
- 99% of thymocytes have detectable levels of CD3 compared to only 75% in wild-type thymocytes
- virtually all CD4 T cells in the periphery express the transgenic TCR
- abnormal T cell proliferation (MGI Ref ID J:85777)
- T cells proliferate strongly when presented the carboxy-terminal fragment of pigeon cytochrome C antigen by autologous spleen cells
- abnormal positive T cell selection (MGI Ref ID J:85777)
- the transgenic TCR is positively selected for on this genetic background (H2-Ek) leading to a more efficient positive T cell selection that is skewed towards CD4 T cell production
- abnormal thymocyte activation (MGI Ref ID J:85777)
- thymocytes proliferate strongly when presented the carboxy-terminal fragment of pigeon cytochrome C antigen by autologous spleen cells
- decreased double-positive T cell number (MGI Ref ID J:85777)
- the percentage of double-positive T cells in the thymus is 24% compared to 83% in wild-type mice
- abnormal T cell subpopulation ratio (MGI Ref ID J:85777)
- the CD4/CD8 single positive T cell ratio in the thymus is heavily skewed towards the CD4 population
- decreased CD8-positive T cell number (MGI Ref ID J:85777)
- there is a two-fold reduction in CD8 single positive T cells in the thymus
- CD8 T cells are severely reduced in peripheral lymph nodes
- increased CD4-positive T cell number (MGI Ref ID J:85777)
- the percentage of CD4 single positive T cells in the thymus is 63% compared to 13% in wild-type mice
- the percentage of CD 4 T cells in lymph nodes is 74% compared to 44% in wild-type controls
- hematopoietic system phenotype
- abnormal T cell differentiation (MGI Ref ID J:85777)
- 75% of thymocytes express high levels of CD3 compared to 18% of wild-type thymocytes
- 99% of thymocytes have detectable levels of CD3 compared to only 75% in wild-type thymocytes
- virtually all CD4 T cells in the periphery express the transgenic TCR
- abnormal T cell proliferation (MGI Ref ID J:85777)
- T cells proliferate strongly when presented the carboxy-terminal fragment of pigeon cytochrome C antigen by autologous spleen cells
- abnormal positive T cell selection (MGI Ref ID J:85777)
- the transgenic TCR is positively selected for on this genetic background (H2-Ek) leading to a more efficient positive T cell selection that is skewed towards CD4 T cell production
- abnormal thymocyte activation (MGI Ref ID J:85777)
- thymocytes proliferate strongly when presented the carboxy-terminal fragment of pigeon cytochrome C antigen by autologous spleen cells
- decreased double-positive T cell number (MGI Ref ID J:85777)
- the percentage of double-positive T cells in the thymus is 24% compared to 83% in wild-type mice
- abnormal T cell subpopulation ratio (MGI Ref ID J:85777)
- the CD4/CD8 single positive T cell ratio in the thymus is heavily skewed towards the CD4 population
- decreased CD8-positive T cell number (MGI Ref ID J:85777)
- there is a two-fold reduction in CD8 single positive T cells in the thymus
- CD8 T cells are severely reduced in peripheral lymph nodes
- increased CD4-positive T cell number (MGI Ref ID J:85777)
- the percentage of CD4 single positive T cells in the thymus is 63% compared to 13% in wild-type mice
- the percentage of CD 4 T cells in lymph nodes is 74% compared to 44% in wild-type controls
This mouse can be used to support research in many areas including:
Tcra relatedImmunology and Inflammation Research
Rearranged Antigen-Specific T Cell Receptor Transgenes (class II restricted)
Research Tools
Immunology and Inflammation Research (T Cell Receptor Transgenics)
Tcrb relatedHematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Research Tools
Cancer Research (specific T cell deficiency)
Hematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(TcrAND)53Hed | ||
|---|---|---|---|
| Allele Name | transgene insertion 53, Stephen M Hedrick | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | AD10TCR-Tg; AND TCR; CC; TcrAND; Tg(TcrAND); | ||
| Mutation Made By | Stephen Hedrick, University of California at San Diego | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Expressed Gene | Tcrb, T-cell receptor beta chain, mouse, laboratory | ||
| Expressed Gene | Tcra, T-cell receptor alpha chain, mouse, laboratory | ||
| General Note | The transgenic TCR is expressed on virtually all T cells in mice expressing Ek. Transgenic mice on a background that involves C57BL/10, C57BL/6, and SJL show expression of a rearranged T-cell receptor specific for the carboxy-terminal fragmentof pigeon cytochrome c and the Ek molecule, resulting in a major subpopulation of T cells restricted to class II MHC proteins. These mice have an abnormally high percentage of mature CD4+CD8- cells, a peripheral T-cell population is almost exclusively CD4+ and show no expression of I-Ek. | ||
| Molecular Note | Rearranged TCR alpha and beta genes containing endogenous promoter and enhancer sequences were coinjected into the micronucleus of 1 cell embryos. Both constructs are specific for the carboxyterminal fragment of pigeon cytochrome c and the Ekmolecule. [MGI Ref ID J:85777] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(TcrAND)53Hed, QPCR, vers. 3
Tg(TcrAND)53Hed, STD PCR, vers. 2
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Kaye J; Hsu ML; Sauron ME; Jameson SC; Gascoigne NR; Hedrick SM. 1989. Selective development of CD4+ T cells in transgenic mice expressing a class II MHC-restricted antigen receptor. Nature 341(6244):746-9. [PubMed: 2571940] [MGI Ref ID J:85777]
Additional References
Tg(TcrAND)53Hed relatedAmsen D; Antov A; Jankovic D; Sher A; Radtke F; Souabni A; Busslinger M; McCright B; Gridley T; Flavell RA. 2007. Direct regulation of gata3 expression determines the T helper differentiation potential of notch. Immunity 27(1):89-99. [PubMed: 17658279] [MGI Ref ID J:123631]
Azzam HS; DeJarnette JB; Huang K; Emmons R; Park CS; Sommers CL; El-Khoury D; Shores EW; Love PE. 2001. Fine tuning of TCR signaling by CD5. J Immunol 166(9):5464-72. [PubMed: 11313384] [MGI Ref ID J:106735]
Babbe H; Chester N; Leder P; Reizis B. 2007. The Bloom's syndrome helicase is critical for development and function of the alphabeta T-cell lineage. Mol Cell Biol 27(5):1947-59. [PubMed: 17210642] [MGI Ref ID J:118992]
Barbee SD; Alberola-Ila J. 2006. Phosphatidylinositol 3-kinase improves the efficiency of positive selection. Int Immunol 18(6):921-30. [PubMed: 16636016] [MGI Ref ID J:109137]
Barndt RJ; Dai M; Zhuang Y. 2000. Functions of E2A-HEB heterodimers in T-cell development revealed by a dominant negative mutation of HEB. Mol Cell Biol 20(18):6677-85. [PubMed: 10958665] [MGI Ref ID J:120411]
Beisner DR; Chu IH; Arechiga AF; Hedrick SM; Walsh CM. 2003. The requirements for Fas-associated death domain signaling in mature T cell activation and survival. J Immunol 171(1):247-56. [PubMed: 12817005] [MGI Ref ID J:132831]
Bourgeois C; Kassiotis G; Stockinger B. 2005. A major role for memory CD4 T cells in the control of lymphopenia-induced proliferation of naive CD4 T cells. J Immunol 174(9):5316-23. [PubMed: 15843528] [MGI Ref ID J:128883]
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Boyer O; Marodon G; Cohen JL; Lejeune L; Irinopoulou T; Liblau R; Bruneval P; Klatzmann D. 2002. Human CD4 expression at the late single-positive stage of thymic development supports T cell maturation and peripheral export in CD4-deficient mice. J Immunol 169(8):4347-53. [PubMed: 12370367] [MGI Ref ID J:120171]
Cannons JL; Yu LJ; Jankovic D; Crotty S; Horai R; Kirby M; Anderson S; Cheever AW; Sher A; Schwartzberg PL. 2006. SAP regulates T cell-mediated help for humoral immunity by a mechanism distinct from cytokine regulation. J Exp Med 203(6):1551-65. [PubMed: 16754717] [MGI Ref ID J:124375]
Cemerski S; Das J; Giurisato E; Markiewicz MA; Allen PM; Chakraborty AK; Shaw AS. 2008. The balance between T cell receptor signaling and degradation at the center of the immunological synapse is determined by antigen quality. Immunity 29(3):414-22. [PubMed: 18760640] [MGI Ref ID J:139670]
Cemerski S; Das J; Locasale J; Arnold P; Giurisato E; Markiewicz MA; Fremont D; Allen PM; Chakraborty AK; Shaw AS. 2007. The stimulatory potency of T cell antigens is influenced by the formation of the immunological synapse. Immunity 26(3):345-55. [PubMed: 17346997] [MGI Ref ID J:120073]
Chambers CA; Kuhns MS; Allison JP. 1999. Cytotoxic T lymphocyte antigen-4 (CTLA-4) regulates primary and secondary peptide-specific CD4(+) T cell responses. Proc Natl Acad Sci U S A 96(15):8603-8. [PubMed: 10411922] [MGI Ref ID J:119845]
Chen Y; Akirav EM; Chen W; Henegariu O; Moser B; Desai D; Shen JM; Webster JC; Andrews RC; Mjalli AM; Rothlein R; Schmidt AM; Clynes R; Herold KC. 2008. RAGE ligation affects T cell activation and controls T cell differentiation. J Immunol 181(6):4272-8. [PubMed: 18768885] [MGI Ref ID J:139083]
Chen YT; Kung JT. 2005. CD1d-independent developmental acquisition of prompt IL-4 gene inducibility in thymus CD161(NK1)-CD44lowCD4+CD8- T cells is associated with complementarity determining region 3-diverse and biased Vbeta2/Vbeta7/Vbeta8/Valpha3.2 T cell receptor usage. J Immunol 175(10):6537-50. [PubMed: 16272308] [MGI Ref ID J:119395]
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Dabbagh K; Dahl ME; Stepick-Biek P; Lewis DB. 2002. Toll-like receptor 4 is required for optimal development of Th2 immune responses: role of dendritic cells. J Immunol 168(9):4524-30. [PubMed: 11970998] [MGI Ref ID J:113992]
De Rosa V; Procaccini C; Cali G; Pirozzi G; Fontana S; Zappacosta S; La Cava A; Matarese G. 2007. A Key Role of Leptin in the Control of Regulatory T Cell Proliferation. Immunity 26(2):241-255. [PubMed: 17307705] [MGI Ref ID J:118425]
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Florido M; Pearl JE; Solache A; Borges M; Haynes L; Cooper AM; Appelberg R. 2005. Gamma interferon-induced T-cell loss in virulent Mycobacterium avium infection. Infect Immun 73(6):3577-86. [PubMed: 15908387] [MGI Ref ID J:99143]
Frank GD; Parnes JR. 1998. The level of CD4 surface protein influences T cell selection in the thymus. J Immunol 160(2):634-42. [PubMed: 9551897] [MGI Ref ID J:45168]
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Huang J; Lo PF; Zal T; Gascoigne NR; Smith BA; Levin SD; Grey HM. 2002. CD28 plays a critical role in the segregation of PKC theta within the immunologic synapse. Proc Natl Acad Sci U S A 99(14):9369-73. [PubMed: 12077322] [MGI Ref ID J:126523]
Huang Y; Comiskey EO; Dupree RS; Li S; Koleske AJ; Burkhardt JK. 2008. The c-Abl tyrosine kinase regulates actin remodeling at the immune synapse. Blood 112(1):111-9. [PubMed: 18305217] [MGI Ref ID J:137330]
Huang Z; Xin J; Coleman J; Huang H. 2005. IFN-gamma suppresses STAT6 phosphorylation by inhibiting its recruitment to the IL-4 receptor. J Immunol 174(3):1332-7. [PubMed: 15661890] [MGI Ref ID J:96417]
Hundt M; Tabata H; Jeon MS; Hayashi K; Tanaka Y; Krishna R; De Giorgio L; Liu YC; Fukata M; Altman A. 2006. Impaired activation and localization of LAT in anergic T cells as a consequence of a selective palmitoylation defect. Immunity 24(5):513-22. [PubMed: 16713970] [MGI Ref ID J:113357]
Jordan MS; Smith JE; Burns JC; Austin JE; Nichols KE; Aschenbrenner AC; Koretzky GA. 2008. Complementation in trans of altered thymocyte development in mice expressing mutant forms of the adaptor molecule SLP76. Immunity 28(3):359-69. [PubMed: 18342008] [MGI Ref ID J:132995]
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Tatari-Calderone Z; Brogdon JL; Tinsley KW; Ramezani A; Leitenberg D. 2005. CD4-dependent signaling is required for a late checkpoint during Th2 development associated with resistance to activation-induced cell death. J Immunol 175(9):5629-36. [PubMed: 16237052] [MGI Ref ID J:119360]
Uehara S; Hayes SM; Li L; El-Khoury D; Canelles M; Fowlkes BJ; Love PE. 2006. Premature expression of chemokine receptor CCR9 impairs T cell development. J Immunol 176(1):75-84. [PubMed: 16365398] [MGI Ref ID J:126252]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry This strain is maintained by homozygous sibling matings. Expected coat color from breeding:Albino Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $104.80 Female or Male Homozygous for Tg(TcrAND)53Hed *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $209.60 Homozygous for Tg(TcrAND)53Hed x Homozygous for Tg(TcrAND)53Hed
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $136.30 Female or Male Homozygous for Tg(TcrAND)53Hed *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $272.50 Homozygous for Tg(TcrAND)53Hed x Homozygous for Tg(TcrAND)53Hed
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 100012 B6SJLF1/J | (approximate) | |
| 000664 C57BL/6J | (approximate) | |
| 000686 SJL/J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. THE LABORATORY EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.
No Liability
In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.
MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.
Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.