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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
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Description
Strain Information
Former Names B6;SJL-Tg(WapHRAS)69Lln YSJL/J (Changed: 15-DEC-04 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation F28 Donating Investigator Loretta Nielsen, Amyllin Pharmaceuticals Inc. Description
The transgene integrated into the Y chromosome. These transgenics serve as models for male mammary cancer and ras-mediated solid tumors. The transgene also can be used as a Y-chromosome marker. On the B6,SJL background males develop multiple mammary tumors and salivary gland tumors by one year of age. The salivary tumors are adenocarcinomas arising from serous areas of the submandibular gland. The mammary gland tumors are adenosquamous carcinomas. Microscopic lung metastases were present in 14% of tumor bearing animals. On the FVB background males develop multiple mammary tumors at 6-8 weeks. The tumors are adenosquamous carcinomas with multiple foci of squamous differentiation or adenocarcinomas containing glandular tissue. There is no metastasis observed.Development
This transgenic strain was developed in the laboratory of Dr. Loretta Nielsen at Schering-Plough Research, Bloomfield, New Jersey. This strain was originally identified as Wap-ras line 69-2.
Control Information
| Control | ||
|---|---|---|
| Noncarrier female | ||
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(Wap-HRAS)69Lln allele
002410 FVB.Cg-Tg(Wap-HRAS)69Lln Chr YSJL/J View Strains carrying Tg(Wap-HRAS)69Lln (1 strain)
006403 129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J
003552 B6129-Tg(Wap-cre)11738Mam/J 003189 C57BL/6J-Tg(WAPTAg)3Knw/J 002500 C57BL/6J-Tg(WapIgf1)39Dlr/J 003188 C57BL/6J-Tg(WapTAg)1Knw 003078 FVB.Cg-Tg(WapIgf1)39Dlr/J 002678 FVB.Cg-Tg(WapTgfa)215Bri/J 002755 FVB/N-Tg(WapNotch4)10Rnc/J
Phenotype
Phenotype Information
Currently there is no phenotype information for this strain.This mouse can be used to support research in many areas including:HRAS related
Cancer Research
Increased Tumor Incidence (Mammary Gland Tumors: males only)
Oncogenes
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Wap-HRAS)69Lln | ||
|---|---|---|---|
| Allele Name | transgene insertion 69, Loretta L Nielsen | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Tg(Wap-HRAS)69Lin; Tg(WapHRAS)69Lin; | ||
| Mutation Made By | Loretta Nielsen, Amyllin Pharmaceuticals Inc. | ||
| Strain of Origin | (C57BL/6 x SJL)F1 | ||
| Expressed Gene | HRAS, v-Ha-ras Harvey rat sarcoma viral oncogene homolog, human | ||
| Promoter | Wap, whey acidic protein, mouse, laboratory | ||
| General Note |
On a genetic background that involves C57BL/6, FVB, and SJL, transgenic male mice develop multiple mammary tumors at 6-8 weeks. The tumors are adenosquamous carcinomas with multiple foci of squamous differentiation or adenocarcinomas containing glandular tissue. No metastasis has been observed. On a background that involves C57BL/6 and SJL, transgenic male mice develop multiple mammary tumors and salivary gland tumors by one year of age. The mammary gland tumors are adenosquamous carcinomas. The salivary tumors are adenocarcinomas arising from serous areas of the submandibular gland. In 14% of these tumor bearing animals microscopic lung metastases are present. | ||
| Molecular Note | This transgene contains the murine whey acidic protein promoter and an allele of the human Harvey rat sarcoma viral oncogene gene homolog that contains a point mutation in codon 12. The transgene integrated into the Y chromosome (SJL). [MGI Ref ID J:72262] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Wap-HRAS)69Lln, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Andres AC; Schonenberger CA; Groner B; Henninghausen L; LeMeur M; Gerlinger P. 1987. Ha-ras oncogene expression directed by a milk protein gene promoter: tissue specificity, hormonal regulation, and tumor induction in transgenic mice. Proc Natl Acad Sci U S A 84(5):1299-303. [PubMed: 3469670] [MGI Ref ID J:72262]
Additional References
Nielsen LL; Discafani CM; Gurnani M; Tyler RD. 1991. Histopathology of salivary and mammary gland tumors in transgenic mice expressing a human Ha-ras oncogene. Cancer Res 51(14):3762-7. [PubMed: 2065330] [MGI Ref ID J:46458]
Nielsen LL; Gurnani M; Catino JJ; Tyler RD. 1995. In wap-ras transgenic mice, tumor phenotype but not cyclophosphamide-sensitivity is affected by genetic background. Anticancer Res 15(2):385-92. [PubMed: 7763010] [MGI Ref ID J:46464]
Nielsen LL; Gurnani M; Tyler RD. 1992. Evaluation of the wap-ras transgenic mouse as a model system for testing anticancer drugs. Cancer Res 52(13):3733-8. [PubMed: 1617645] [MGI Ref ID J:46461]
Tg(Wap-HRAS)69Lln relatedBremer C; Ntziachristos V; Weitkamp B; Theilmeier G; Heindel W; Weissleder R. 2005. Optical imaging of spontaneous breast tumors using protease sensing 'smart' optical probes. Invest Radiol 40(6):321-7. [PubMed: 15905717] [MGI Ref ID J:101113]
Nielsen LL; Discafani CM; Gurnani M; Tyler RD. 1991. Histopathology of salivary and mammary gland tumors in transgenic mice expressing a human Ha-ras oncogene. Cancer Res 51(14):3762-7. [PubMed: 2065330] [MGI Ref ID J:46458]
Nielsen LL; Gurnani M; Catino JJ; Tyler RD. 1995. In wap-ras transgenic mice, tumor phenotype but not cyclophosphamide-sensitivity is affected by genetic background. Anticancer Res 15(2):385-92. [PubMed: 7763010] [MGI Ref ID J:46464]
Nielsen LL; Gurnani M; Porter G; Trexler S; Emerson D; Tyler RD. 1994. Development of a nude mouse model of ras-mediated neoplasia using WR21 cells from a transgenic mouse salivary tumor. In Vivo 8(3):295-302. [PubMed: 7803707] [MGI Ref ID J:46463]
Nielsen LL; Gurnani M; Tyler RD. 1992. Evaluation of the wap-ras transgenic mouse as a model system for testing anticancer drugs. Cancer Res 52(13):3733-8. [PubMed: 1617645] [MGI Ref ID J:46461]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier female | ||
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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