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Strain Name:

FVB/N-Tg(MtTGFA)100Lmb/J

Stock Number:

002421

Availability:

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Genes & Alleles   Mt1;   TGFA;   Tg(MtTGFA)100Lmb;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Strain
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Glenn Merlino,   National Institutes of Health

Appearance
albino
Related Genotype: Tyrc/Tyrc

Strain Description
In the CD1-TgN(MtTGFA)42Lmb line liver lesions develop in a progressive fashion, with neoplasia confirmed at autopsy (average age 13 months). Mammary ductal network was more densely developed in CD1-TgN(MtTGFA)42Lmb transgenic mice, with dividing cells in the subtending ducts as well as in the end bud region. Pancreas showed progressive hyperplasia of the stroma, tubular structures and ductular metaplasia. Penetrance was 100%. The mice were not diabetic. There was no obvious inflammatory reaction. Mice from the FVB/N-TgN(MtTGFA)100Lmb line exhibit a 60% mammary tumor incidence.

Mammalian Phenotype Terms assigned by genotype

Tg(MtTGFA)100Lmb/0

        involves: FVB/N
  • endocrine/exocrine gland phenotype
  • abnormal mammary gland morphology (MGI Ref ID J:72083)
    • secretory epithelium cell survival and proliferation is increased during involution compared to in wild type mice
    • abnormal involution of the mammary gland (MGI Ref ID J:72083)
      • involution is delayed compared to in wild type mice
  • tumorigenesis
  • mammary gland tumor (MGI Ref ID J:72083)
    • by 9 months of age, 62% of female mice develop mammary tumors with or without zinc induction
    • mammary gland tumors develop mostly in parous females and only 1 male exhibited a mammary gland tumor
    • however, not evidence of metastatic lesions are present
    • mammary adenocarcinoma (MGI Ref ID J:72083)
      • by 9 months of age, one third of multiparous females develop mammary adenocarcinomas
  • reproductive system phenotype
  • abnormal mammary gland morphology (MGI Ref ID J:72083)
    • secretory epithelium cell survival and proliferation is increased during involution compared to in wild type mice
    • abnormal involution of the mammary gland (MGI Ref ID J:72083)
      • involution is delayed compared to in wild type mice

Tg(MtTGFA)100Lmb/Tg(MtTGFA)100Lmb

        involves: FVB/N
  • digestive/alimentary phenotype
  • pancreatic fibrosis (MGI Ref ID J:28452)
    • in young mice the pancreas becomes uniformly rigid and whitish as interstitial fibrosis occurs
  • endocrine/exocrine gland phenotype
  • pancreatic fibrosis (MGI Ref ID J:28452)
    • in young mice the pancreas becomes uniformly rigid and whitish as interstitial fibrosis occurs

Gene & Allele Details

Allele Symbol Tg(MtTGFA)100Lmb
Allele Name transgene insertion 100, Glenn Merlino
Mutation Made By Glenn Merlino,   National Institutes of Health
Strain of OriginFVB/N
Expressed Gene TGFA, transforming growth factor, alpha, human
Promoter Mt1, metallothionein 1, mouse, laboratory
Molecular Note This transgene contains the inducible mouse metallothionein 1 promoter, cDNA of the wild-type allele of the human transforming growth factor alpha gene, and a human growth hormone polyadenylation signal. Line 100 carries ten copies of the transgene [MGI Ref ID J:28452]

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(MtTGFA)

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying other alleles of Mt1     (10 strains)

Strains carrying other alleles of TGFA
002459   B6D2-Tg(MMTVTGFA)254Rjc/J
002373   B6D2-Tg(MMTVTGFA)29Rjc/J
002953   FVB.Cg-Tg(MMTVTGFA)254Rjc/J
View Strains carrying other alleles of TGFA     (3 strains)

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence (Mammary Gland Tumors)

Mt1 related

Metabolism Research

References

Selected Reference(s)

Jhappan C; Stahle C; Harkins RN; Fausto N; Smith GH; Merlino GT. 1990. TGF alpha overexpression in transgenic mice induces liver neoplasia and abnormal development of the mammary gland and pancreas. Cell 61(6):1137-46. [PubMed: 2350785]  [MGI Ref ID J:28452]

Additional References

Price and Supply Information

Strain Name: FVB/N-Tg(MtTGFA)100Lmb/J
Stock Number: 002421

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

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Control InformationView Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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