Strain Name:

C3H/He-Tg(LCKprBCL2)36Sjk/J

Stock Number:

002427

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Stanley J. Korsmeyer,   Dana-Farber Cancer Institute

Important Note
This strain does not carry mouse mammary tumor virus (MMTV).

Description
Hemizygotes carrying the human LCKprBCL2 transgene display normal architecture of all lymphoid organs to 10 weeks of age. They show an increased percentage of CD3hi/TCRhi and CD4-8+ thymocytes with a decreased percentage in CD3lo T cells. CD8+ cells and the total percentage of T cells are increased in the spleen and lymph nodes. Mice are resistant to apoptosis induced by glucocorticoid treatment, by radiation treatment, or by anti-CD3 treatment. Malignant lymphoma develop in hemizygotes at approximately 18 months of age.

Control Information

  Control
   Noncarrier
   000659 C3H/HeJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of BCL2
002319   B6.Cg-Tg(BCL2)22Wehi/J
002320   B6.Cg-Tg(BCL2)25Wehi/J
002321   B6.Cg-Tg(BCL2)36Wehi/J
002318   C.Cg-Tg(BCL2)22Wehi/J
002971   FVB-Tg(BCL2OVARY)1Ah/J
View Strains carrying other alleles of BCL2     (5 strains)

View Strains carrying other alleles of Lck     (12 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested.
B-Cell Cll/Lymphoma 2; BCL2   (BCL2)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(LCKprBCL2)36Sjk/?

        involves: C3H * C57BL/6
  • immune system phenotype
  • abnormal T cell number
    • at 12 months of age, there is a 3-4 fold increase in the absolute number of T cells in the spleen   (MGI Ref ID J:28635)
    • transgenic mice have 83 x 106 T cells compared to 25 x 106 T cells   (MGI Ref ID J:28635)
    • abnormal T cell subpopulation ratio
      • ratio of CD4 to CD8 T cells in the spleen is 1.4 compared to 2.6 in control mice   (MGI Ref ID J:11548)
    • increased T cell number
      • CD3high/TCRhigh cells make up 40% of thymocytes compared to 15% in non-transgenic controls   (MGI Ref ID J:11548)
      • there is a reciprocal decrease in CD3lo cells in the thymus   (MGI Ref ID J:11548)
      • the number of T cells found in the spleen doubles   (MGI Ref ID J:11548)
      • increased CD8-positive T cell number
        • the percentage of CD8 single positive T cells found in the thymus is almost double that in wild-type   (MGI Ref ID J:11548)
        • the percentage of CD8 T cells in the spleen is also doubled   (MGI Ref ID J:11548)
  • abnormal thymus cell ratio
    • ratio of CD4 to CD8 single positive T cells is 1.3 in transgenic mice compared to 3.9 in wild-type mice   (MGI Ref ID J:11548)
  • decreased T cell apoptosis
    • 30% of thymocytes survive 6 days in culture compared to less than 1% for thymocytes from wild-type mice due to less apoptosis   (MGI Ref ID J:11548)
    • similar results are seen for splenic cells   (MGI Ref ID J:11548)
    • apoptosis induced by dexamethasone only causes a10-20% reduction in the number of thymocytes compared to 90 to 98% in control mice   (MGI Ref ID J:11548)
    • thymocytes are also resistant to apoptosis induced by low-dose radiation   (MGI Ref ID J:11548)
    • thymocytes are resistant to apoptosis triggered by CD3 ligation   (MGI Ref ID J:11548)
  • tumorigenesis
  • increased T cell derived lymphoma incidence
    • 18 of 68 mice develop lymphoma at a mean age of 18 months compared to 1 wild-type mouse out of 56 developing lymphoma in the control group   (MGI Ref ID J:28635)
    • 15 of the 18 lymphomas are large cell lymphoma with several showing immunoblastic features   (MGI Ref ID J:28635)
    • the remaining 3 are diffuse small lymphocytic lymphoma   (MGI Ref ID J:28635)
    • the lymphomas usually present as a rapidly expanding mass in the mesenteric lymph nodes though they can also occur in the mediastinal or cervical nodes   (MGI Ref ID J:28635)
    • extranodal involvement in other tissues occurs frequently   (MGI Ref ID J:28635)
    • splenomegaly and ascites fluid are sometimes observed   (MGI Ref ID J:28635)
  • hematopoietic system phenotype
  • abnormal T cell number
    • at 12 months of age, there is a 3-4 fold increase in the absolute number of T cells in the spleen   (MGI Ref ID J:28635)
    • transgenic mice have 83 x 106 T cells compared to 25 x 106 T cells   (MGI Ref ID J:28635)
    • abnormal T cell subpopulation ratio
      • ratio of CD4 to CD8 T cells in the spleen is 1.4 compared to 2.6 in control mice   (MGI Ref ID J:11548)
    • increased T cell number
      • CD3high/TCRhigh cells make up 40% of thymocytes compared to 15% in non-transgenic controls   (MGI Ref ID J:11548)
      • there is a reciprocal decrease in CD3lo cells in the thymus   (MGI Ref ID J:11548)
      • the number of T cells found in the spleen doubles   (MGI Ref ID J:11548)
      • increased CD8-positive T cell number
        • the percentage of CD8 single positive T cells found in the thymus is almost double that in wild-type   (MGI Ref ID J:11548)
        • the percentage of CD8 T cells in the spleen is also doubled   (MGI Ref ID J:11548)
  • abnormal thymus cell ratio
    • ratio of CD4 to CD8 single positive T cells is 1.3 in transgenic mice compared to 3.9 in wild-type mice   (MGI Ref ID J:11548)
  • decreased T cell apoptosis
    • 30% of thymocytes survive 6 days in culture compared to less than 1% for thymocytes from wild-type mice due to less apoptosis   (MGI Ref ID J:11548)
    • similar results are seen for splenic cells   (MGI Ref ID J:11548)
    • apoptosis induced by dexamethasone only causes a10-20% reduction in the number of thymocytes compared to 90 to 98% in control mice   (MGI Ref ID J:11548)
    • thymocytes are also resistant to apoptosis induced by low-dose radiation   (MGI Ref ID J:11548)
    • thymocytes are resistant to apoptosis triggered by CD3 ligation   (MGI Ref ID J:11548)
  • cellular phenotype
  • decreased T cell apoptosis
    • 30% of thymocytes survive 6 days in culture compared to less than 1% for thymocytes from wild-type mice due to less apoptosis   (MGI Ref ID J:11548)
    • similar results are seen for splenic cells   (MGI Ref ID J:11548)
    • apoptosis induced by dexamethasone only causes a10-20% reduction in the number of thymocytes compared to 90 to 98% in control mice   (MGI Ref ID J:11548)
    • thymocytes are also resistant to apoptosis induced by low-dose radiation   (MGI Ref ID J:11548)
    • thymocytes are resistant to apoptosis triggered by CD3 ligation   (MGI Ref ID J:11548)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Apoptosis Research
Endogenous Regulators

Cancer Research
Increased Tumor Incidence
      Lymphomas

Immunology, Inflammation and Autoimmunity Research
Intracellular Signaling Molecules

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(LCKprBCL2)36Sjk
Allele Name transgene insertion 36, Stanley J Korsmeyer
Allele Type Transgenic (random, expressed)
Common Name(s) Lck-Bcl-2; lckpr-bcl-2; prLck.hbcl-2;
Mutation Made ByDr. Stanley Korsmeyer,   Dana-Farber Cancer Institute
Expressed Gene BCL2, B-cell CLL/lymphoma 2, human
Promoter Lck, lymphocyte protein tyrosine kinase, mouse, laboratory
Molecular Note The transgene consists of 0.75 kb of Lck proximal promoter sequences (active in lymphoid tissues) and 3.2 kb of human BCL2 cDNA. Introns, exons, and polyadenylation sequences from the human growth hormone gene consitutes the 3' untranslated region of theconstruct. Transgene expression in the thymus, lymph nodes, and spleen was verified by Western blot analysis and immunohistochemistry. Seven transgenic lines were established with lines 7, 17, and 36 expressing the transgene at high levels. [MGI Ref ID J:11548]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(BCL2),

MELT



Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Sentman CL; Shutter JR; Hockenbery D; Kanagawa O; Korsmeyer SJ. 1991. bcl-2 inhibits multiple forms of apoptosis but not negative selection in thymocytes. Cell 67(5):879-88. [PubMed: 1835668]  [MGI Ref ID J:11548]

Additional References

Linette GP; Hess JL; Sentman CL; Korsmeyer SJ. 1995. Peripheral T-cell lymphoma in lckpr-bcl-2 transgenic mice. Blood 86(4):1255-60. [PubMed: 7632929]  [MGI Ref ID J:28635]

Tg(LCKprBCL2)36Sjk related

Albu DI; Feng D; Bhattacharya D; Jenkins NA; Copeland NG; Liu P; Avram D. 2007. BCL11B is required for positive selection and survival of double-positive thymocytes. J Exp Med 204(12):3003-15. [PubMed: 17998389]  [MGI Ref ID J:128515]

Babbe H; Chester N; Leder P; Reizis B. 2007. The Bloom's syndrome helicase is critical for development and function of the alphabeta T-cell lineage. Mol Cell Biol 27(5):1947-59. [PubMed: 17210642]  [MGI Ref ID J:118992]

Bhandoola A; Dolnick B; Fayad N; Nussenzweig A; Singer A. 2000. Immature thymocytes undergoing receptor rearrangements are resistant to an Atm-dependent death pathway activated in mature T cells by double-stranded DNA breaks. J Exp Med 192(6):891-7. [PubMed: 10993919]  [MGI Ref ID J:112013]

Cancro MP; Sah AP; Levy SL; Allman DM; Schmidt MR; Woodland RT. 2001. xid mice reveal the interplay of homeostasis and Bruton's tyrosine kinase-mediated selection at multiple stages of B cell development. Int Immunol 13(12):1501-14. [PubMed: 11717191]  [MGI Ref ID J:109858]

Chao DT; Linette GP; Boise LH; White LS; Thompson CB; Korsmeyer SJ. 1995. Bcl-XL and Bcl-2 repress a common pathway of cell death. J Exp Med 182(3):821-8. [PubMed: 7650488]  [MGI Ref ID J:78583]

Cheng N; van de Wetering CI; Knudson CM. 2008. p27 deficiency cooperates with Bcl-2 but not Bax to promote T-cell lymphoma. PLoS ONE 3(4):e1911. [PubMed: 18382684]  [MGI Ref ID J:134259]

Chi TH; Wan M; Lee PP; Akashi K; Metzger D; Chambon P; Wilson CB; Crabtree GR. 2003. Sequential roles of Brg, the ATPase subunit of BAF chromatin remodeling complexes, in thymocyte development. Immunity 19(2):169-82. [PubMed: 12932351]  [MGI Ref ID J:85191]

Hsu FC; Pajerowski AG; Nelson-Holte M; Sundsbak R; Shapiro VS. 2011. NKAP is required for T cell maturation and acquisition of functional competency. J Exp Med 208(6):1291-304. [PubMed: 21624937]  [MGI Ref ID J:176824]

Huang Z; Xie H; Ioannidis V; Held W; Clevers H; Sadim MS; Sun Z. 2006. Transcriptional regulation of CD4 gene expression by T cell factor-1/beta-catenin pathway. J Immunol 176(8):4880-7. [PubMed: 16585583]  [MGI Ref ID J:131156]

Ioannidis V; Beermann F; Clevers H; Held W. 2001. The beta-catenin--TCF-1 pathway ensures CD4(+)CD8(+) thymocyte survival. Nat Immunol 2(8):691-7. [PubMed: 11477404]  [MGI Ref ID J:107212]

Linette GP; Grusby MJ; Hedrick SM; Hansen TH; Glimcher LH; Korsmeyer SJ. 1994. Bcl-2 is upregulated at the CD4+ CD8+ stage during positive selection and promotes thymocyte differentiation at several control points. Immunity 1(3):197-205. [PubMed: 7889408]  [MGI Ref ID J:81144]

Linette GP; Hess JL; Sentman CL; Korsmeyer SJ. 1995. Peripheral T-cell lymphoma in lckpr-bcl-2 transgenic mice. Blood 86(4):1255-60. [PubMed: 7632929]  [MGI Ref ID J:28635]

Linette GP; Li Y; Roth K; Korsmeyer SJ. 1996. Cross talk between cell death and cell cycle progression: BCL-2 regulates NFAT-mediated activation. Proc Natl Acad Sci U S A 93(18):9545-52. [PubMed: 8790367]  [MGI Ref ID J:153272]

Linowes BA; Ligons DL; Nam AS; Hong C; Keller HR; Tai X; Luckey MA; Park JH. 2013. Pim1 permits generation and survival of CD4(+) T cells in the absence of gammac cytokine receptor signaling. Eur J Immunol 43(9):2283-94. [PubMed: 23712827]  [MGI Ref ID J:201196]

Liu X; Bosselut R. 2004. Duration of TCR signaling controls CD4-CD8 lineage differentiation in vivo. Nat Immunol 5(3):280-8. [PubMed: 14770180]  [MGI Ref ID J:88469]

Matei IR; Gladdy RA; Nutter LM; Canty A; Guidos CJ; Danska JS. 2007. ATM deficiency disrupts Tcra locus integrity and the maturation of CD4+CD8+ thymocytes. Blood 109(5):1887-96. [PubMed: 17077325]  [MGI Ref ID J:145360]

McCaughtry TM; Etzensperger R; Alag A; Tai X; Kurtulus S; Park JH; Grinberg A; Love P; Feigenbaum L; Erman B; Singer A. 2012. Conditional deletion of cytokine receptor chains reveals that IL-7 and IL-15 specify CD8 cytotoxic lineage fate in the thymus. J Exp Med 209(12):2263-76. [PubMed: 23109710]  [MGI Ref ID J:190889]

Nakajima H; Leonard WJ. 1997. Impaired peripheral deletion of activated T cells in mice lacking the common cytokine receptor gamma-chain: defective Fas ligand expression in gamma-chain-deficient mice. J Immunol 159(10):4737-44. [PubMed: 9366397]  [MGI Ref ID J:43962]

Nakajima H; Leonard WJ. 1999. Role of Bcl-2 in alpha beta T cell development in mice deficient in the common cytokine receptor gamma-chain: the requirement for Bcl-2 differs depending on the TCR/MHC affinity. J Immunol 162(2):782-90. [PubMed: 9916699]  [MGI Ref ID J:52019]

Ohteki T; Maki C; Koyasu S. 2001. Overexpression of Bcl-2 differentially restores development of thymus-derived CD4-8+ T cells and intestinal intraepithelial T cells in IFN-regulatory factor-1-deficient mice. J Immunol 166(11):6509-13. [PubMed: 11359801]  [MGI Ref ID J:69487]

Park JH; Adoro S; Guinter T; Erman B; Alag AS; Catalfamo M; Kimura MY; Cui Y; Lucas PJ; Gress RE; Kubo M; Hennighausen L; Feigenbaum L; Singer A. 2010. Signaling by intrathymic cytokines, not T cell antigen receptors, specifies CD8 lineage choice and promotes the differentiation of cytotoxic-lineage T cells. Nat Immunol 11(3):257-64. [PubMed: 20118929]  [MGI Ref ID J:158504]

Peng S; Lalani S; Leavenworth JW; Ho IC; Pauza ME. 2007. c-Maf interacts with c-Myb to down-regulate Bcl-2 expression and increase apoptosis in peripheral CD4 cells. Eur J Immunol 37(10):2868-80. [PubMed: 17823980]  [MGI Ref ID J:125265]

Peng S; Wu H; Mo YY; Watabe K; Pauza ME. 2009. c-Maf increases apoptosis in peripheral CD8 cells by transactivating Caspase 6. Immunology 127(2):267-78. [PubMed: 19476513]  [MGI Ref ID J:155649]

Pobezinsky LA; Angelov GS; Tai X; Jeurling S; Van Laethem F; Feigenbaum L; Park JH; Singer A. 2012. Clonal deletion and the fate of autoreactive thymocytes that survive negative selection. Nat Immunol 13(6):569-78. [PubMed: 22544394]  [MGI Ref ID J:186451]

Tai X; Cowan M; Feigenbaum L; Singer A. 2005. CD28 costimulation of developing thymocytes induces Foxp3 expression and regulatory T cell differentiation independently of interleukin 2. Nat Immunol 6(2):152-62. [PubMed: 15640801]  [MGI Ref ID J:95668]

Tamayo E; Postigo J; Del Giudice G; Rappuoli R; Benito A; Yagita H; Merino R; Merino J. 2009. Involvement of the intrinsic and extrinsic cell-death pathways in the induction of apoptosis of mature lymphocytes by the Escherichia coli heat-labile enterotoxin. Eur J Immunol 39(2):439-46. [PubMed: 19180465]  [MGI Ref ID J:144472]

Tarrant TK; Silver PB; Wahlsten JL; Rizzo LV; Chan CC; Wiggert B; Caspi RR. 1999. Interleukin 12 protects from a T helper type 1-mediated autoimmune disease, experimental autoimmune uveitis, through a mechanism involving interferon gamma, nitric oxide, and apoptosis. J Exp Med 189(2):219-30. [PubMed: 9892605]  [MGI Ref ID J:119614]

Thapa P; Das J; McWilliams D; Shapiro M; Sundsbak R; Nelson-Holte M; Tangen S; Anderson J; Desiderio S; Hiebert S; Sant'angelo DB; Shapiro VS. 2013. The transcriptional repressor NKAP is required for the development of iNKT cells. Nat Commun 4:1582. [PubMed: 23481390]  [MGI Ref ID J:205750]

Thompson J; Winoto A. 2008. During negative selection, Nur77 family proteins translocate to mitochondria where they associate with Bcl-2 and expose its proapoptotic BH3 domain. J Exp Med 205(5):1029-36. [PubMed: 18443228]  [MGI Ref ID J:136238]

Thompson LF; Van de Wiele CJ; Laurent AB; Hooker SW; Vaughn JG; Jiang H; Khare K; Kellems RE; Blackburn MR; Hershfield MS; Resta R. 2000. Metabolites from apoptotic thymocytes inhibit thymopoiesis in adenosine deaminase-deficient fetal thymic organ cultures. J Clin Invest 106(9):1149-57. [PubMed: 11067867]  [MGI Ref ID J:115112]

Tikhonova AN; Van Laethem F; Hanada K; Lu J; Pobezinsky LA; Hong C; Guinter TI; Jeurling SK; Bernhardt G; Park JH; Yang JC; Sun PD; Singer A. 2012. alphabeta T Cell Receptors that Do Not Undergo Major Histocompatibility Complex-Specific Thymic Selection Possess Antibody-like Recognition Specificities. Immunity 36(1):79-91. [PubMed: 22209676]  [MGI Ref ID J:180750]

Tinsley KW; Hong C; Luckey MA; Park JY; Kim GY; Yoon HW; Keller HR; Sacks AJ; Feigenbaum L; Park JH. 2013. Ikaros is required to survive positive selection and to maintain clonal diversity during T-cell development in the thymus. Blood 122(14):2358-68. [PubMed: 23908463]  [MGI Ref ID J:203282]

Van De Wiele CJ; Joachims ML; Fesler AM; Vaughn JG; Blackburn MR; McGee ST; Thompson LF. 2006. Further differentiation of murine double-positive thymocytes is inhibited in adenosine deaminase-deficient murine fetal thymic organ culture. J Immunol 176(10):5925-33. [PubMed: 16670300]  [MGI Ref ID J:131709]

Van Laethem F; Tikhonova AN; Pobezinsky LA; Tai X; Kimura MY; Le Saout C; Guinter TI; Adams A; Sharrow SO; Bernhardt G; Feigenbaum L; Singer A. 2013. Lck availability during thymic selection determines the recognition specificity of the T cell repertoire. Cell 154(6):1326-41. [PubMed: 24034254]  [MGI Ref ID J:204615]

Van de Wiele CJ; Marino JH; Tan C; Kneale HA; Weber J; Morelli JN; Davis BK; Taylor AA; Teague TK. 2007. Impaired thymopoiesis in interleukin-7 receptor transgenic mice is not corrected by Bcl-2. Cell Immunol 250(1-2):31-9. [PubMed: 18321477]  [MGI Ref ID J:133580]

van de Wetering CI; Knudson CM. 2007. Chromosomal instability and supernumerary centrosomes represent precursor defects in a mouse model of T-cell lymphoma. Cancer Res 67(17):8081-8. [PubMed: 17804719]  [MGI Ref ID J:124884]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
   000659 C3H/HeJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Important Note

This strain does not carry mouse mammary tumor virus (MMTV).

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MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.5)