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Strain Name:

C3H/He-Tg(LCKprBCL2)36Sjk/J

Stock Number:

002427

Availability:

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Genes & Alleles   BCL2;   Lck;   Tg(LCKprBCL2)36Sjk;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Strain
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Stanley Korsmeyer,   Dana-Farber Cancer Institute

Important Note
This strain does not carry mouse mammary tumor virus (MMTV).

Strain Description
Hemizygotes carrying the human LCKprBCL2 transgene display normal architecture of all lymphoid organs to 10 weeks of age. They show an increased percentage of CD3hi/TCRhi and CD4-8+ thymocytes with a decreased percentage in CD3lo T cells. CD8+ cells and the total percentage of T cells are increased in the spleen and lymph nodes. Mice are resistant to apoptosis induced by glucocorticoid treatment, by radiation treatment, or by anti-CD3 treatment. Malignant lymphoma develop in hemizygotes at approximately 18 months of age.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(LCKprBCL2)36Sjk/?

        involves: C3H * C57BL/6
  • immune system phenotype
  • abnormal T cell number (MGI Ref ID J:28635)
    • at 12 months of age, there is a 3-4 fold increase in the absolute number of T cells in the spleen
    • transgenic mice have 83 x 106 T cells compared to 25 x 106 T cells
    • abnormal T cell subpopulation ratio (MGI Ref ID J:11548)
      • ratio of CD4 to CD8 T cells in the spleen is 1.4 compared to 2.6 in control mice
    • increased T cell number (MGI Ref ID J:11548)
      • CD3high/TCRhigh cells make up 40% of thymocytes compared to 15% in non-transgenic controls
      • there is a reciprocal decrease in CD3lo cells in the thymus
      • the number of T cells found in the spleen doubles
      • increased CD8-positive T cell number (MGI Ref ID J:11548)
        • the percentage of CD8 single positive T cells found in the thymus is almost double that in wild-type
        • the percentage of CD8 T cells in the spleen is also doubled
  • abnormal thymus cell ratio (MGI Ref ID J:11548)
    • ratio of CD4 to CD8 single positive T cells is 1.3 in transgenic mice compared to 3.9 in wild-type mice
  • decreased T cell apoptosis (MGI Ref ID J:11548)
    • 30% of thymocytes survive 6 days in culture compared to less than 1% for thymocytes from wild-type mice due to less apoptosis
    • similar results are seen for splenic cells
    • apoptosis induced by dexamethasone only causes a10-20% reduction in the number of thymocytes compared to 90 to 98% in control mice
    • thymocytes are also resistant to apoptosis induced by low-dose radiation
    • thymocytes are resistant to apoptosis triggered by CD3 ligation
  • tumorigenesis
  • T cell derived lymphoma (MGI Ref ID J:28635)
    • 18 of 68 mice develop lymphoma at a mean age of 18 months compared to 1 wild-type mouse out of 56 developing lymphoma in the control group
    • 15 of the 18 lymphomas are large cell lymphoma with several showing immunoblastic features
    • the remaining 3 are diffuse small lymphocytic lymphoma
    • the lymphomas ussulay present as a rapidly expanding mass in the mesenteric lymph nodes though they can also occur in the mediastinal or cervical nodes
    • extranodal involvement in other tissues occurs frequently
    • splenomegaly and ascites fluid are sometimes observed
  • hematopoietic system phenotype
  • abnormal T cell number (MGI Ref ID J:28635)
    • at 12 months of age, there is a 3-4 fold increase in the absolute number of T cells in the spleen
    • transgenic mice have 83 x 106 T cells compared to 25 x 106 T cells
    • abnormal T cell subpopulation ratio (MGI Ref ID J:11548)
      • ratio of CD4 to CD8 T cells in the spleen is 1.4 compared to 2.6 in control mice
    • increased T cell number (MGI Ref ID J:11548)
      • CD3high/TCRhigh cells make up 40% of thymocytes compared to 15% in non-transgenic controls
      • there is a reciprocal decrease in CD3lo cells in the thymus
      • the number of T cells found in the spleen doubles
      • increased CD8-positive T cell number (MGI Ref ID J:11548)
        • the percentage of CD8 single positive T cells found in the thymus is almost double that in wild-type
        • the percentage of CD8 T cells in the spleen is also doubled
  • abnormal thymus cell ratio (MGI Ref ID J:11548)
    • ratio of CD4 to CD8 single positive T cells is 1.3 in transgenic mice compared to 3.9 in wild-type mice
  • decreased T cell apoptosis (MGI Ref ID J:11548)
    • 30% of thymocytes survive 6 days in culture compared to less than 1% for thymocytes from wild-type mice due to less apoptosis
    • similar results are seen for splenic cells
    • apoptosis induced by dexamethasone only causes a10-20% reduction in the number of thymocytes compared to 90 to 98% in control mice
    • thymocytes are also resistant to apoptosis induced by low-dose radiation
    • thymocytes are resistant to apoptosis triggered by CD3 ligation

Gene & Allele Details

Allele Symbol Tg(LCKprBCL2)36Sjk
Allele Name transgene insertion 36, Stanley J Korsmeyer
Common Name(s) Lck-Bcl-2; lckpr-bcl-2;
Mutation Made By Stanley Korsmeyer,   Dana-Farber Cancer Institute
Expressed Gene BCL2, B-cell CLL/lymphoma 2, human
Promoter Lck, lymphocyte protein tyrosine kinase, mouse, laboratory
Molecular Note The transgene consists of 0.75 kb of Lck proximal promoter sequences (active in lymphoid tissues) and 3.2 kb of human BCL2 cDNA. Introns, exons, and polyadenylation sequences from the human growth hormone gene consitutes the 3' untranslated region of theconstruct. Transgene expression in the thymus, lymph nodes, and spleen was verified by Western blot analysis and immunohistochemistry. Seven transgenic lines were established with lines 7, 17, and 36 expressing the transgene at high levels. [MGI Ref ID J:11548]

Control Information

  Control
   Noncarrier
   000659 C3H/HeJ
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(BCL2)

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying other alleles of BCL2
002319   B6.Cg-Tg(BCL2)22Wehi/J
002320   B6.Cg-Tg(BCL2)25Wehi/J
002321   B6.Cg-Tg(BCL2)36Wehi/J
002318   C.Cg-Tg(BCL2)22Wehi/J
002971   FVB-Tg(BCL2OVARY)1Ah/J
View Strains carrying other alleles of BCL2     (5 strains)

Strains carrying other alleles of Lck
002817   B6.129S2-Lcktm1Mak/J
003802   B6.Cg-Tg(Lck-cre)548Jxm/J
003332   C57BL/6-Tg(LckNotch1)9Erob/J
002230   C57BL/6J-Tg(LckIl4)1315Dbl/J
005732   NOD.Cg-Tg(Lck-cre)548Jxm/AchJ
View Strains carrying other alleles of Lck     (5 strains)

Research Applications

This mouse can be used to support research in many areas including:

Apoptosis Research
Endogenous Regulators

Cancer Research
Increased Tumor Incidence (Lymphomas)

Immunology and Inflammation Research
Intracellular Signaling Molecules

References

Selected Reference(s)

Sentman CL; Shutter JR; Hockenbery D; Kanagawa O; Korsmeyer SJ. 1991. bcl-2 inhibits multiple forms of apoptosis but not negative selection in thymocytes. Cell 67(5):879-88. [PubMed: 1835668]  [MGI Ref ID J:11548]

Additional References

Price and Supply Information

Strain Name: C3H/He-Tg(LCKprBCL2)36Sjk/J
Stock Number: 002427

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

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Control InformationView Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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