Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N2   Donating Investigator Stuart Orkin,   Harvard Medical School

Appearance
white-bellied agouti
Related Genotype: A^w/A^w

Description
Mice homozygous for the Tal1^tm1Sho mutation die between embryonic day 9.5 and 10.5. There is a complete absence of blood formation; no hematopoietic cells could be identified by appearance, histology, colony assays, or RT-PCR for lineage-specific products.

Control Information

	Control
	Wild-type from the colony
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying   Tal1^tm1Sho allele

002948

B6.129S4-Tal1tm1Sho/J

View Strains carrying   Tal1^tm1Sho     (1 strain)

Strains carrying other alleles of Tal1

006209

FVB.Cg-Tg(Tal1-tTA)19Dgt/J

View Strains carrying other alleles of Tal1     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Tal1^tm1Sho/Tal1^tm1Sho

        involves: 129S4/SvJae

• lethality-prenatal/perinatal
• embryonic lethality during organogenesis (MGI Ref ID J:23061)
  • homozygotes are obtained at the expected Mendelian frequency at E8.5 but die by E10.5
• embryogenesis phenotype
• abnormal embryonic hematopoiesis (MGI Ref ID J:23061)
  • at E9.5-E10, mutant embryos display total absence of embryonic (nucleated) red cells
• abnormal placental labyrinth vasculature morphology (MGI Ref ID J:23061)
  • at E9.5, homozygotes show absence of embryonic (nucleated) blood cells within endothelial-lined capillaries in the fetal labyrinth
• absent blood islands (MGI Ref ID J:23061)
  • at E9.5-E10, mutant yolk sacs show absence of nucleated red blood cells
• embryonic growth retardation (MGI Ref ID J:23061)
  • at E9.5-E10, homozygotes exhibit a delay in overall growth
• incomplete somite formation (MGI Ref ID J:23061)
  • at E9.5, homozygotes exhibit an average of 21 somite pairs vs >25 pairs found in wild-type embryos
• reduced embryo size (MGI Ref ID J:23061)
  • at E9.5-E10, homozygotes are easily distinguished by their smaller size
• hematopoietic system phenotype
• abnormal embryonic hematopoiesis (MGI Ref ID J:23061)
  • at E9.5-E10, mutant embryos display total absence of embryonic (nucleated) red cells
• absent common myeloid progenitor cells (MGI Ref ID J:23061)
  • in vitro, yolk sac cells obtained from E8.5-E9.0 homozygotes show a virtual absence of myeloid colony formation, suggesting a deficit at the myelo-erythroid progenitor level
• anemia (MGI Ref ID J:23061)
  • at E9.5-E10, homozygotes are extremely anemic
• cardiovascular system phenotype
• distended pericardial sacs (MGI Ref ID J:23061)
  • at E9.5-E10, homozygotes display dilated pericardial sacs
• growth/size phenotype
• embryonic growth retardation (MGI Ref ID J:23061)
  • at E9.5-E10, homozygotes exhibit a delay in overall growth
• reduced embryo size (MGI Ref ID J:23061)
  • at E9.5-E10, homozygotes are easily distinguished by their smaller size
• skin/coat/nails phenotype
• pallor (MGI Ref ID J:23061)
  • at E9.5-E10, homozygotes are extremely pale
• cellular phenotype
• necrosis (MGI Ref ID J:23061)
  • at E9.5-E10, homozygotes display extensive necrosis

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Tal1^tm1Sho related

Developmental Biology Research
Internal/Organ Defects (hematopoietic defects)

Hematological Research
Hematopoietic Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tal1tm1Sho
Allele Name		targeted mutation 1, Stuart Orkin
Allele Type		Targeted (knock-out)
Common Name(s)		SCL; tal-1-/-;
Mutation Made By		Stuart Orkin,   Harvard Medical School
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Tal1, T-cell acute lymphocytic leukemia 1
Chromosome		4
Gene Common Name(s)		SCL; SCL/tal-1; Scl; TCL5; bHLHa17; homolog of human stem-cell leukemia gene (prov.); tal-1;
Molecular Note		Replacement of coding sequences of the Tal1 gene, including the bHLH sequences, with a neomycin cassette. [MGI Ref ID J:23061]

Genotyping

Genotyping Information

Genotyping Protocols

Tal1^tm1Sho, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Shivdasani RA; Mayer EL; Orkin SH. 1995. Absence of blood formation in mice lacking the T-cell leukaemia oncoprotein tal-1/SCL. Nature 373(6513):432-4. [PubMed: 7830794]  [MGI Ref ID J:23061]

Additional References

Tal1^tm1Sho related

D'Souza SL; Elefanty AG; Keller G. 2005. SCL/Tal-1 is essential for hematopoietic commitment of the hemangioblast but not for its development. Blood 105(10):3862-70. [PubMed: 15677567]  [MGI Ref ID J:98970]

Nishimura S; Takahashi S; Kuroha T; Suwabe N; Nagasawa T; Trainor C; Yamamoto M. 2000. A GATA box in the GATA-1 gene hematopoietic enhancer is a critical element in the network of GATA factors and sites that regulate this gene. Mol Cell Biol 20(2):713-23. [PubMed: 10611250]  [MGI Ref ID J:59206]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Wild-type from the colony
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

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