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Strain Name:

C3.129P2(B6)-B2mtm1Unc/J

Stock Number:

002439

Availability:

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General Terms and Conditions

Former Name      C3.129P2(B6)-B2mtm1Unc    (Changed: 15-DEC-04 )
Genes & Alleles   B2m;   B2mtm1Unc;   Pde6b;   Pde6brd1;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Specieslaboratory mouse
Background Strain C3H/HeJ
Donor Strain B6;129P-B2mtm1Unc (129P2 derived E14TG2a ES cell line)
Donating Investigator Derry Roopenian,   The Jackson Laboratory
GenerationN11F5pN1

Appearance
agouti
Related Genotype: A/A

Important Note
This strain is homozygous for the retinal degeneration allele Pde6brd1.

Strain Description
Mice homozygous for the B2mtm1Unc targeted mutation have little if any MHC class I protein expression on the cell surface. There are few CD8+ cytotoxic T-cells and under some circumstances a compensatory increase in CD4+ cytotoxic T-cells. Immune responses involving CD8+T-cells are severely deficient providing a model to assess the role of CD8+ cells and class I MHC in various experimental systems. Hemachromatosis has been noted in certain genetic backgrounds (Rothenberg BE, Voland JR, Proc Natl Acad Sci USA 93:1529-34, 1996). In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Strain Development
The B2mtm1Unc mutant strain was developed in the laboratory of Dr. Beverly Koller and Dr. Oliver Smithies at the University of North Carolina at Chapel Hill. It was generated by a targeted disruption of the B2m gene. The 129-derived E14TG2a ES cell line was used. The C3H/HeJ strain was produced in the laboratory of Dr. Derry Roopenian at The Jackson Laboratory by backcrossing the B2mtm1Unc mutation 10 times to C3H/HeJ inbred mice.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

B2mtm1Unc/B2m+

        involves: 129P2/OlaHsd * C57BL/6
  • immune system phenotype
  • decreased CD8-positive T cell number (MGI Ref ID J:64451)
    • numbers of CD8+CD4- T cells from the thymus of five week old mice are reduced in comparison to control (4.9% vs. 6.7%), however, in peripheral lymphoid tissues numbers of CD8+CD4- T cells do not differ from control
  • decreased level of surface class I molecules (MGI Ref ID J:64451)
    • surface expression of class I molecules in cells from lymph nodes are reduced in comparison to control
  • hematopoietic system phenotype
  • decreased CD8-positive T cell number (MGI Ref ID J:64451)
    • numbers of CD8+CD4- T cells from the thymus of five week old mice are reduced in comparison to control (4.9% vs. 6.7%), however, in peripheral lymphoid tissues numbers of CD8+CD4- T cells do not differ from control

B2mtm1Unc/B2mtm1Unc

        involves: 129P2/OlaHsd * C57BL/6
  • hematopoietic system phenotype
  • absent CD8-positive T cells (MGI Ref ID J:64451)
    • deficient in CD4-CD8+ T cells
    • CD8+CD4- T cells from the thymus of five week old mice are almost non-existent in comparison to control (0.8% vs. 6.7%), however, total numbers of alpha beta T cells and CD8-CD4+ T cells do not differ significantly from control
  • immune system phenotype
  • absent CD8-positive T cells (MGI Ref ID J:64451)
    • deficient in CD4-CD8+ T cells
    • CD8+CD4- T cells from the thymus of five week old mice are almost non-existent in comparison to control (0.8% vs. 6.7%), however, total numbers of alpha beta T cells and CD8-CD4+ T cells do not differ significantly from control
  • decreased level of surface class I molecules (MGI Ref ID J:64451)
    • surface expression of class I molecules in cells from lymph nodes, spleen and thymus cannot be detected by flow cytometry

B2mtm1Unc/B2mtm1Unc

        B6.129P2-B2mtm1Unc/J
  • life span-post-weaning/aging
  • abnormal induced morbidity/mortality (MGI Ref ID J:123934)
    • following infection with either a low or high dose of IOE, 90% of mice survive a low dose and all mice succumb to a high dose
  • immune system phenotype
  • abnormal interleukin level (MGI Ref ID J:123934)
    • following infection with a lethal low dose of a monocytotropic Ehrlichia bacteria from Ixodes ovatrus ticks (IOE), induces less IL-10 than in infected wild type mice
  • abnormal tumor necrosis factor level (MGI Ref ID J:123934)
    • following infection with a lethal low dose of a monocytotropic Ehrlichia bacteria from Ixodes ovatrus ticks (IOE), induces less TNF-alpha than in infected wild type mice
  • decreased CD8-positive T cell number (MGI Ref ID J:123934)
    • following infection with a monocytotropic Ehrlichia bacteria from Ixodes ovatrus ticks (IOE), CD8+ T cells in the spleen are reduced (1.5%+/-0.1% of splenocytes compared to 9.7%+/-0.7% of splenocytes in wild type mice)
    • in uninfected mice the number of CD8+ T cells in the spleen is reduced compared to in wild type mice
  • decreased susceptibility to autoimmune diabetes (MGI Ref ID J:135214)
    • mice do not develop spontaneous diabetes compared to wild-type NOD controls
    • when splenocytes from diabetic donors are transferred into lethally-irradiated 8-week old recipients, female mice remain normoglycemic for about 17 weeks before developing hyperglycemia/diabetes; kinetics are much slower than in B2m-null, Tg(GFAP-B2m)Mdos (line 9 or 14) transgenic mice
  • decreased susceptibility to bacterial infection (MGI Ref ID J:123934)
    • mice are highly resistant to infection with a monocytotropic Ehrlichia bacteria from Ixodes ovatrus ticks (IOE) following infection with either a low or high dose of IOE, 90% of mice survive a low dose with mild illness and all mice succumb to a high dose
    • following infection with IEO, CD8+ T cells in the spleen are reduced (1.5%+/-0.1% of splenocytes compared to 9.7%+/-0.7% of splenocytes in wild type mice)
    • mice have lower burdens of Ehrlichia bacteria in the lungs and spleen following infection than do wild type mice
    • at day 12 and 14 post-infection with a lethal low dose of IEO, mice exhibit only mild liver pathology, few apoptotic foci in the liver and preserved lymphoid tissue cellularity
  • increased T-helper 1 cell number (MGI Ref ID J:123934)
    • following infection with a monocytotropic Ehrlichia bacteria from Ixodes ovatrus ticks (IOE), the number of interferon-gamma producing CD4+ Th1 cells in the spleen on day 8 and day 12 is increased relative to in infected wild type mice
  • hematopoietic system phenotype
  • decreased CD8-positive T cell number (MGI Ref ID J:123934)
    • following infection with a monocytotropic Ehrlichia bacteria from Ixodes ovatrus ticks (IOE), CD8+ T cells in the spleen are reduced (1.5%+/-0.1% of splenocytes compared to 9.7%+/-0.7% of splenocytes in wild type mice)
    • in uninfected mice the number of CD8+ T cells in the spleen is reduced compared to in wild type mice
  • increased T-helper 1 cell number (MGI Ref ID J:123934)
    • following infection with a monocytotropic Ehrlichia bacteria from Ixodes ovatrus ticks (IOE), the number of interferon-gamma producing CD4+ Th1 cells in the spleen on day 8 and day 12 is increased relative to in infected wild type mice
  • digestive/alimentary phenotype
  • abnormal pancreas morphology (MGI Ref ID J:135214)
    • mice develop peri-islet T cell aggregates >30 weeks of age
  • endocrine/exocrine gland phenotype
  • abnormal pancreas morphology (MGI Ref ID J:135214)
    • mice develop peri-islet T cell aggregates >30 weeks of age
  • homeostasis/metabolism phenotype
  • abnormal interleukin level (MGI Ref ID J:123934)
    • following infection with a lethal low dose of a monocytotropic Ehrlichia bacteria from Ixodes ovatrus ticks (IOE), induces less IL-10 than in infected wild type mice
  • abnormal tumor necrosis factor level (MGI Ref ID J:123934)
    • following infection with a lethal low dose of a monocytotropic Ehrlichia bacteria from Ixodes ovatrus ticks (IOE), induces less TNF-alpha than in infected wild type mice

Gene & Allele Details

Allele Symbol B2mtm1Unc
Allele Name targeted mutation 1, University of North Carolina
Common Name(s) I0; b2mnull; beta2-m-KO; beta2M-; beta2MKO; beta2m0; beta2mnull; beta2mo; beta2mtm1Unc;
Mutation Made By Oliver Smithies,   University of North Carolina
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14TG2a
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name B2m, beta-2 microglobulin
Chromosome 2
Gene Common Name(s) Ly-m11; beta 2 microglobulin; beta2-m; lymphocyte antigen m11;
Molecular Note Insertion of a neomycin-resistance gene into the second exon. [MGI Ref ID J:65612]
 
Allele Symbol Pde6brd1
Allele Name retinal degeneration 1
Common Name(s) rd; rd-1; rd1; rodless retina;

Control Information

  Control
   000659 C3H/HeJ
 
  Considerations for Choosing Controls

Genotyping Protocols

B2mtm1Unc

Colony Maintenance

Breeding & HusbandryThis B2mtm1Unc strain is maintained by mating homozygous siblings. Only homozygous mice may be ordered.

Related Strains

View Strains carrying   B2mtm1Unc     (19 strains)

Strains carrying   Pde6brd1 allele
004202   B6.C3 Pde6brd1 Hps4le/+ +-Lmx1adr-8J/J
000002   B6.C3-Pde6brd1 Hps4le/J
001022   B6C3FeF1/J a/a
000652   BDP/J
000653   BUB/BnJ
005494   C3.129S1(B6)-Grm1rcw/J
000480   C3.MRL-Faslpr/J
001957   C3A Pde6brd1.O20/A-Prph2Rd2/J
005973   C3Bir.129P2(B6)-Il10C3Bir/LtJ
004326   C3Bir.129P2(B6)-Il10tm1Cgn/Lt
003968   C3Bir.129P2(B6)-Il10tm1Cgn/LtJ
001906   C3Ga.Cg-Catb/J
001904   C3H-Atcayji-hes/J
000659   C3H/HeJ
000784   C3H/HeJ-Faslgld/J
000509   C3H/HeJ-Lystbg-2J/J
002433   C3H/HeJ-Spnb4qv-lnd2J/J
005972   C3H/HeJBirLtJ
001824   C3H/HeJSxJ
000635   C3H/HeOuJ
000474   C3H/HeSn
001431   C3H/HeSn-ocd/J
000661   C3H/HeSnJ
002235   C3H/HeSnJ-Ctnna2cdf/J
002333   C3H/HeSnJ-gri/J
006435   C3HeB.SW-Soaa/MonJ
000658   C3HeB/FeJ
001576   C3HeB/FeJ-Atp7btx-J/J
002588   C3HeB/FeJ-Eya1bor/J
001533   C3HeB/FeJ-Mc1rE-so Gli3Xt-J/J
001886   C3HeB/FeJLe a/a-gnd/J
001908   C3HfB/BiJ
001502   C3Sn.B6-Epha4rb/J
001547   C3Sn.Cg-Cm/J
000656   CBA/J
000813   CBA/J-Atp7aMo-pew/J
000660   DA/HuSnJ
000023   FL/1ReJ
000025   FL/4ReJ
003024   FVB.129P2(B6)-Fmr1tm1Cgr/J
002539   FVB.129P2-Abcb4tm1Bor/J
002935   FVB.129S2(B6)-Ccnd1tm1Wbg/J
002953   FVB.Cg-Tg(MMTVTGFA)254Rjc/J
003170   FVB.Cg-Tg(Myh6-tTA)6Smbf/J
003078   FVB.Cg-Tg(WapIgf1)39Dlr/J
003257   FVB/N-Tg(GFAPGFP)14Mes/J
002374   FVB/N-Tg(MMTV-PyVT)634Mul/J
002856   FVB/N-Tg(TIE2-lacZ)182Sato/J
002384   FVB/N-Tg(UcpDta)1Kz/J
001800   FVB/NJ
003487   FVB/NJ-Tg(XGFAP-lacZ)3Mes/J
001491   FVB/NMob
000734   MOLD/RkJ
000550   MOLF/EiJ
002423   NON/ShiLtJ
000679   P/J
000680   PL/J
100299   PLSJLF1/J
000269   SB/LeJ
005651   SJL.AK-Thy1a/TseJ
000686   SJL/J
000688   ST/bJ
004808   STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
002648   STOCK a/a Cln6nclf/J
000279   STOCK gr +/+ Ap3d1mh/J
005965   STOCK Tg(Pomc1-cre)16Lowl/J
004770   SW.B6-Soab/J
002023   SWR.M-Emv21 Emv22/J
000689   SWR/J
000939   SWR/J-Clcn1adr-mto/J
000692   WB/ReJ KitW/J
100410   WBB6F1/J-KitW/KitW-v/J
000693   WC/ReJ KitlSl/J
100401   WCB6F1/J KitlSl KitlSl-d
View Strains carrying   Pde6brd1     (74 strains)

View Strains carrying other alleles of Pde6b     (8 strains)

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

B2mtm1Unc related

Hematological Research
Anemia, Iron Deficiency and Transport Defects (hemochromatosis)

Immunology and Inflammation Research
Immunodeficiency (MHC class I deficiency)

Internal/Organ Research
Liver Defects (hemochromatosis)

Metabolism Research
Hemochromatosis (iron metabolism defects)

Research Tools
Immunology and Inflammation Research (MHC class I deficiency)

Pde6brd1 related

Mouse/Human Gene Homologs
retinitis pigmentosa, autosomal recessive

Sensorineural Research
Retinal Degeneration

References

Selected Reference(s)

Koller BH; Marrack P; Kappler JW; Smithies O. 1990. Normal development of mice deficient in beta 2M, MHC class I proteins, and CD8+ T cells. Science 248(4960):1227-30. [PubMed: 2112266]  [MGI Ref ID J:64451]

Additional References

Price and Supply Information

Strain Name: C3.129P2(B6)-B2mtm1Unc/J
Stock Number: 002439

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

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Control InformationView Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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