Strain Name:

B6;129S-Rab3atm1Sud/J

Stock Number:

002443

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Thomas C. Sudhof,   Stanford University School of Medicine

Description
Mice homozygous for the Rab3atm1Sud targeted mutation are viable, fertile, and comparable in weight and health to normal wild type siblings. Most of their synaptic parameters are normal, although synaptic depression after repetitive stimulation is significantly increased, suggesting a role for Rab3a in synaptic vesicle docking. Mutant mice do not express Rab3a and levels of rabphilin (a protein that associates with the GTP-bound form of Rab3) are decreased by 70%. Expression of over 20 other synaptic proteins is unchanged.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Rab3atm1Sud allele
006375   B6;129-Rab3btm1Sud Rab3atm1Sud Rab3dtm1Rja Rab3ctm1Sud/J
View Strains carrying   Rab3atm1Sud     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Rab3atm1Sud/Rab3a+

        involves: 129 * C57BL/6J
  • behavior/neurological phenotype
  • shortened circadian period
    • 23.45 +/- 0.04 hours for heterozygotes on a mixed B6;129 genetic background compared to 23.71 +/-0.03 hours of control littermates   (MGI Ref ID J:79562)

Rab3atm1Sud/Rab3atm1Sud

        involves: 129/Sv
  • nervous system phenotype
  • increased synaptic depression
    • significant increase in synaptic depression of transmission after short trains of repetitive stimuli (15-30 stimuli at 14 Hz) in sections of the hippocampus   (MGI Ref ID J:64297)

Rab3atm1Sud/Rab3atm1Sud

        involves: 129 * C57BL/6J
  • behavior/neurological phenotype
  • shortened circadian period
    • 23.39 +/- 0.09 hours for homozygotes on a mixed B6;129 genetic background compared to 23.71 +/-0.03 hours of control littermates   (MGI Ref ID J:79562)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Rab3atm1Sud related

Cancer Research
Oncogenes

Neurobiology Research
Neurotransmitter Receptor and Synaptic Vesicle Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Rab3atm1Sud
Allele Name targeted mutation 1, Thomas C Sudhof
Allele Type Targeted (Null/Knockout)
Common Name(s) Rab3a-;
Mutation Made ByDr. Thomas Sudhof,   Stanford University School of Medicine
Strain of Origin129
ES Cell Line Strain129
Gene Symbol and Name Rab3a, RAB3A, member RAS oncogene family
Chromosome 8
Gene Common Name(s) RAB3;
Molecular Note A neomycin cassette replaced the promoter and the first two exons of the gene. Western blots of brain extracts did not detect any encoded protein in homozygous mice. [MGI Ref ID J:64297]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Geppert M; Bolshakov VY; Siegelbaum SA; Takei K; De Camilli P; Hammer RE; Sudhof TC. 1994. The role of Rab3A in neurotransmitter release. Nature 369(6480):493-7. [PubMed: 7911226]  [MGI Ref ID J:64297]

Additional References

Rab3atm1Sud related

Bednarek E; Caroni P. 2011. beta-Adducin is required for stable assembly of new synapses and improved memory upon environmental enrichment. Neuron 69(6):1132-46. [PubMed: 21435558]  [MGI Ref ID J:174730]

Blundell J; Kaeser PS; Sudhof TC; Powell CM. 2010. RIM1alpha and interacting proteins involved in presynaptic plasticity mediate prepulse inhibition and additional behaviors linked to schizophrenia. J Neurosci 30(15):5326-33. [PubMed: 20392954]  [MGI Ref ID J:159850]

Castillo PE; Janz R; Sudhof TC; Tzounopoulos T; Malenka RC; Nicoll RA. 1997. Rab3A is essential for mossy fibre long-term potentiation in the hippocampus. Nature 388(6642):590-3. [PubMed: 9252190]  [MGI Ref ID J:42242]

Chang B; Hawes NL; Hurd RE; Wang J; Howell D; Davisson MT; Roderick TH; Nusinowitz S; Heckenlively JR. 2005. Mouse models of ocular diseases. Vis Neurosci 22(5):587-93. [PubMed: 16332269]  [MGI Ref ID J:156373]

Coleman WL; Bill CA; Bykhovskaia M. 2007. Rab3a deletion reduces vesicle docking and transmitter release at the mouse diaphragm synapse. Neuroscience 148(1):1-6. [PubMed: 17640821]  [MGI Ref ID J:128286]

Coleman WL; Bykhovskaia M. 2010. Cooperative regulation of neurotransmitter release by Rab3a and synapsin II. Mol Cell Neurosci 44(2):190-200. [PubMed: 20338242]  [MGI Ref ID J:164134]

D'Adamo P; Wolfer DP; Kopp C; Tobler I; Toniolo D; Lipp HP. 2004. Mice deficient for the synaptic vesicle protein Rab3a show impaired spatial reversal learning and increased explorative activity but none of the behavioral changes shown by mice deficient for the Rab3a regulator Gdi1. Eur J Neurosci 19(7):1895-905. [PubMed: 15078563]  [MGI Ref ID J:89723]

Feliciano P; Andrade R; Bykhovskaia M. 2013. Synapsin II and Rab3a cooperate in the regulation of epileptic and synaptic activity in the CA1 region of the hippocampus. J Neurosci 33(46):18319-30. [PubMed: 24227741]  [MGI Ref ID J:204165]

Geppert M; Goda Y; Stevens CF; Sudhof TC. 1997. The small GTP-binding protein Rab3A regulates a late step in synaptic vesicle fusion. Nature 387(6635):810-4. [PubMed: 9194562]  [MGI Ref ID J:111167]

Giardino L; Armelloni S; Corbelli A; Mattinzoli D; Zennaro C; Guerrot D; Tourrel F; Ikehata M; Li M; Berra S; Carraro M; Messa P; Rastaldi MP. 2009. Podocyte glutamatergic signaling contributes to the function of the glomerular filtration barrier. J Am Soc Nephrol 20(9):1929-40. [PubMed: 19578006]  [MGI Ref ID J:166334]

Gogolla N; Galimberti I; Deguchi Y; Caroni P. 2009. Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus. Neuron 62(4):510-25. [PubMed: 19477153]  [MGI Ref ID J:157356]

Hensbroek RA; Kamal A; Baars AM; Verhage M; Spruijt BM. 2003. Spatial, contextual and working memory are not affected by the absence of mossy fiber long-term potentiation and depression. Behav Brain Res 138(2):215-23. [PubMed: 12527452]  [MGI Ref ID J:96225]

Hirsh JK; Searl TJ; Silinsky EM. 2002. Regulation by Rab3A of an endogenous modulator of neurotransmitter release at mouse motor nerve endings. J Physiol 545(Pt 2):337-43. [PubMed: 12456815]  [MGI Ref ID J:105966]

Kapfhamer D; Bettinger JC; Davies AG; Eastman CL; Smail EA; Heberlein U; McIntire SL. 2008. Loss of RAB-3/A in Caenorhabditis elegans and the mouse affects behavioral response to ethanol. Genes Brain Behav 7(6):669-76. [PubMed: 18397381]  [MGI Ref ID J:151156]

Kapfhamer D; Valladares O; Sun Y; Nolan PM; Rux JJ; Arnold SE; Veasey SC; Bucan M. 2002. Mutations in Rab3a alter circadian period and homeostatic response to sleep loss in the mouse. Nat Genet 32(2):290-5. [PubMed: 12244319]  [MGI Ref ID J:79562]

Leenders AG; Lopes da Silva FH; Ghijsen WE; Verhage M. 2001. Rab3a is involved in transport of synaptic vesicles to the active zone in mouse brain nerve terminals. Mol Biol Cell 12(10):3095-102. [PubMed: 11598194]  [MGI Ref ID J:111329]

Lonart G; Janz R; Johnson KM; Sudhof TC. 1998. Mechanism of action of rab3A in mossy fiber LTP. Neuron 21(5):1141-50. [PubMed: 9856469]  [MGI Ref ID J:110768]

Powell CM; Schoch S; Monteggia L; Barrot M; Matos MF; Feldmann N; Sudhof TC; Nestler EJ. 2004. The presynaptic active zone protein RIM1alpha is critical for normal learning and memory. Neuron 42(1):143-53. [PubMed: 15066271]  [MGI Ref ID J:89753]

Ruediger S; Vittori C; Bednarek E; Genoud C; Strata P; Sacchetti B; Caroni P. 2011. Learning-related feedforward inhibitory connectivity growth required for memory precision. Nature 473(7348):514-8. [PubMed: 21532590]  [MGI Ref ID J:172603]

Schluter OM; Basu J; Sudhof TC; Rosenmund C. 2006. Rab3 superprimes synaptic vesicles for release: implications for short-term synaptic plasticity. J Neurosci 26(4):1239-46. [PubMed: 16436611]  [MGI Ref ID J:104983]

Schluter OM; Schmitz F; Jahn R; Rosenmund C; Sudhof TC. 2004. A complete genetic analysis of neuronal Rab3 function. J Neurosci 24(29):6629-37. [PubMed: 15269275]  [MGI Ref ID J:105478]

Schonn JS; van Weering JR; Mohrmann R; Schluter OM; Sudhof TC; de Wit H; Verhage M; Sorensen JB. 2010. Rab3 proteins involved in vesicle biogenesis and priming in embryonic mouse chromaffin cells. Traffic 11(11):1415-28. [PubMed: 20716109]  [MGI Ref ID J:166046]

Silinsky EM. 2004. Adenosine decreases both presynaptic calcium currents and neurotransmitter release at the mouse neuromuscular junction. J Physiol 558(Pt 2):389-401. [PubMed: 15146054]  [MGI Ref ID J:105453]

Sons MS; Plomp JJ. 2006. Rab3A deletion selectively reduces spontaneous neurotransmitter release at the mouse neuromuscular synapse. Brain Res 1089(1):126-134. [PubMed: 16631140]  [MGI Ref ID J:109669]

Thakker-Varia S; Alder J; Crozier RA; Plummer MR; Black IB. 2001. Rab3A is required for brain-derived neurotrophic factor-induced synaptic plasticity: transcriptional analysis at the population and single-cell levels. J Neurosci 21(17):6782-90. [PubMed: 11517266]  [MGI Ref ID J:123429]

Wang X; Thiagarajan R; Wang Q; Tewolde T; Rich MM; Engisch KL. 2008. Regulation of quantal shape by Rab3A: evidence for a fusion pore-dependent mechanism. J Physiol 586(16):3949-62. [PubMed: 18591190]  [MGI Ref ID J:176387]

Wang X; Wang Q; Yang S; Bucan M; Rich MM; Engisch KL. 2011. Impaired Activity-Dependent Plasticity of Quantal Amplitude at the Neuromuscular Junction of Rab3A Deletion and Rab3A Earlybird Mutant Mice. J Neurosci 31(10):3580-8. [PubMed: 21389214]  [MGI Ref ID J:170082]

Yang S; Farias M; Kapfhamer D; Tobias J; Grant G; Abel T; Bucan M. 2007. Biochemical, molecular and behavioral phenotypes of Rab3A mutations in the mouse. Genes Brain Behav 6(1):77-96. [PubMed: 16734774]  [MGI Ref ID J:132642]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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