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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Thomas Sudhof, U. of Texas, SW Medical Center at Dallas Description
Homozygotes are viable and fertile. Homozygotes show an impaired short-term synaptic plasticity, particulary post-tetanic potentiation. May serve as a model for epilepsy.
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms provided by MGI
assigned by genotype
Syn1tm1Sud/Syn1tm1Sud
involves: 129S7/SvEvBrd
- behavior/neurological phenotype
- convulsive seizures
- seizure activity begins after 2 months of age (MGI Ref ID J:15701)
- nervous system phenotype
- convulsive seizures
- seizure activity begins after 2 months of age (MGI Ref ID J:15701)
- enhanced paired-pulse facilitation
- enhancement of mean paired pulse facilitation (PPF) (MGI Ref ID J:15701)
This mouse can be used to support research in many areas including:Syn1tm1Sud related
Neurobiology Research
Epilepsy
Neurotransmitter Receptor and Synaptic Vesicle Defects
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Syn1tm1Sud | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Thomas C Sudhof | ||
| Allele Type | Targeted (knock-out) | ||
| Mutation Made By | Thomas Sudhof, Stanford University School of Medicine | ||
| Strain of Origin | 129S7/SvEvBrd | ||
| ES Cell Line Name | JH1 | ||
| ES Cell Line Strain | 129S7/SvEvBrd | ||
| Gene Symbol and Name | Syn1, synapsin I | ||
| Chromosome | X | ||
| Gene Common Name(s) | SYN1a; SYN1b; SYNI; Syn-1; Synapsin Ia; Synapsin Ib; | ||
| Molecular Note | A neomycin resistance cassette replaced exon 1 of the gene. Immunoblots of brains from mutant mice showed no detectable protein. Immunocytochemistry of brains from mutant mice showed no detectable protein staining. [MGI Ref ID J:15701] | ||
Genotyping
Genotyping Information
Genotyping Protocols
NEOTD (Generic Neo), Standard PCR
Syn1tm1Sud, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Rosahl TW; Geppert M; Spillane D; Herz J; Hammer RE; Malenka RC; Sudhof TC. 1993. Short-term synaptic plasticity is altered in mice lacking synapsin I. Cell 75(4):661-70. [PubMed: 7902212] [MGI Ref ID J:15701]
Additional References
Rosahl TW; Spillane D; Missler M; Herz J; Selig DK; Wolff JR; Hammer RE; Malenka RC; Sudhof TC. 1995. Essential functions of synapsins I and II in synaptic vesicle regulation [see comments] Nature 375(6531):488-93. [PubMed: 7777057] [MGI Ref ID J:26920]
Spillane DM; Rosahl TW; Sudhof TC; Malenka RC. 1995. Long-term potentiation in mice lacking synapsins. Neuropharmacology 34(11):1573-9. [PubMed: 8606805] [MGI Ref ID J:88303]
Syn1tm1Sud relatedColeman WL; Bill CA; Simsek-Duran F; Lonart G; Samigullin D; Bykhovskaia M. 2008. Synapsin II and calcium regulate vesicle docking and the cross-talk between vesicle pools at the mouse motor terminals. J Physiol 586(Pt 19):4649-73. [PubMed: 18669537] [MGI Ref ID J:176383]
Gabriel T; Garcia-Perez E; Mahfooz K; Goni J; Martinez-Turrillas R; Perez-Otano I; Lo DC; Wesseling JF. 2011. A new kinetic framework for synaptic vesicle trafficking tested in synapsin knock-outs. J Neurosci 31(32):11563-77. [PubMed: 21832187] [MGI Ref ID J:175240]
Hosaka M; Sudhof TC. 1999. Homo- and heterodimerization of synapsins. J Biol Chem 274(24):16747-53. [PubMed: 10358015] [MGI Ref ID J:115251]
Kushner SA; Elgersma Y; Murphy GG; Jaarsma D; van Woerden GM; Hojjati MR; Cui Y; LeBoutillier JC; Marrone DF; Choi ES; De Zeeuw CI; Petit TL; Pozzo-Miller L; Silva AJ. 2005. Modulation of presynaptic plasticity and learning by the H-ras/extracellular signal-regulated kinase/synapsin I signaling pathway. J Neurosci 25(42):9721-34. [PubMed: 16237176] [MGI Ref ID J:102160]
Lonart G; Simsek-Duran F. 2006. Deletion of synapsins I and II genes alters the size of vesicular pools and rabphilin phosphorylation. Brain Res 1107(1):42-51. [PubMed: 16844103] [MGI Ref ID J:111929]
Rosahl TW; Spillane D; Missler M; Herz J; Selig DK; Wolff JR; Hammer RE; Malenka RC; Sudhof TC. 1995. Essential functions of synapsins I and II in synaptic vesicle regulation [see comments] Nature 375(6531):488-93. [PubMed: 7777057] [MGI Ref ID J:26920]
Silva AJ; Rosahl TW; Chapman PF; Marowitz Z; Friedman E; Frankland PW; Cestari V; Cioffi D; Sudhof TC; Bourtchuladze R. 1996. Impaired learning in mice with abnormal short-lived plasticity. Curr Biol 6(11):1509-18. [PubMed: 8939606] [MGI Ref ID J:146734]
Spillane DM; Rosahl TW; Sudhof TC; Malenka RC. 1995. Long-term potentiation in mice lacking synapsins. Neuropharmacology 34(11):1573-9. [PubMed: 8606805] [MGI Ref ID J:88303]
Sun J; Bronk P; Liu X; Han W; Sudhof TC. 2006. Synapsins regulate use-dependent synaptic plasticity in the calyx of Held by a Ca2+/calmodulin-dependent pathway. Proc Natl Acad Sci U S A 103(8):2880-85. [PubMed: 16481620] [MGI Ref ID J:107311]
Tu Y; Nayak SK; Woodson J; Ross EM. 2003. Phosphorylation-regulated inhibition of the Gz GTPase-activating protein activity of RGS proteins by synapsin I. J Biol Chem 278(52):52273-81. [PubMed: 14557263] [MGI Ref ID J:87097]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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