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Strain Name:

B6D2-Tg(MMTVTGFA)254Rjc/J

Stock Number:

002459

Availability:

Repository-Cryopreserved


General Terms and Conditions

Genes & Alleles   TGFA;   MMTV;   Tg(MMTVTGFA)254Rjc;


Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Strain
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Robert Coffey,   Vanderbilt University Medical Center
GenerationN?+2pN1

Strain Description
The phenotype of TgN(MMTVTGFA)254Rjc transgenic mice is very similar to that of B6D2-TgN(MMTVTGFA)29Rjc (Stock No. 002373). Mice are viable and fertile, but lactate insufficiently to support the litter. Virgin transgenic mice show no mammary gland abnormalities prepubertally, but adult virgin mice have considerable alveolar gland hyperplasia. Pregnant transgenic mice show marked proliferation of the stromal cells, and alveolar secretion is markedly increased compared to nontransgenic mice. After multiple pregnancies, isolated adenocarcinomas develop. There is no apparent phenotypic effect in males. In females transgene expression was localized to the small ducts and alveoli in both virgin and pregnant mice as evidenced by in situ hybridization and by immunohistochemistry. Immunostaining also revealed some stromal staining in the hyperplastic areas. Egfr mRNA expression was also increased in mammary tissues expressing high levels of the transgene. Crosses between TGFA transgenic mice and TGFB1 transgenic mice demonstrate that these growth factors oppose each other's action on mammary gland development. The B6D2-TgN(MMTVTGFA)254Rjc line is notable for sebaceous gland hyperplasia, which occurs in both male and female mice. The hyperplasia was evidenced as multiple raised, white plaques and nodules on the abdominal skin. No dysplasia or sebaceous cell carcinoma was observed. High expression of TGFa mRNA and TGFa protein was detected in the areas of sebaceous hyperplasia.

Strain Development
To construct pMMTV-TGFa, a pKCR vector in which the SV40 early promoter region was replaced with complete MMTV LTR and a 925 bp human TGFa cDNA was inserted in the EcoRI site of b-globin exon 3. The 3.6 kb XhoI fragment was microinjected into (C57BL X DBA)F2 fertilized eggs. Transgenic mice are identified either by Southern blot using a b-globin exon 3 probe or by PCR. Transgenic lines were generated by mating founder animals to (C57BL X DBA/2)F1 males and females. No details of subsequent strain development were provided.

Mammalian Phenotype Terms assigned by genotype

Tg(MMTVTGFA)254Rjc/0

        involves: C57BL/6 * DBA/2
  • endocrine/exocrine gland phenotype
  • abnormal mammary gland morphology (MGI Ref ID J:28453)
    • at 2.5 months of age, the virgin female founder displayed duct abnormalities but little alveolar development
    • female mice develop cystic and solid hyperplasias
  • enlarged sebaceous gland (MGI Ref ID J:72085)
    • mice exhibit sebaceous gland hyperplasia
  • tumorigenesis
  • mammary adenocarcinoma (MGI Ref ID J:72085)
  • behavior/neurological phenotype
  • abnormal nursing (MGI Ref ID J:72085)
    • females cannot nurse her young
  • skin/coat/nails phenotype
  • abnormal skin condition (MGI Ref ID J:72085)
    • mice exhibit multiple, raised, white plaques and nodules on the abdominal skin
  • enlarged sebaceous gland (MGI Ref ID J:72085)
    • mice exhibit sebaceous gland hyperplasia
  • reproductive system phenotype
  • abnormal mammary gland morphology (MGI Ref ID J:28453)
    • at 2.5 months of age, the virgin female founder displayed duct abnormalities but little alveolar development
    • female mice develop cystic and solid hyperplasias

Gene & Allele Details

Allele Symbol Tg(MMTVTGFA)254Rjc
Allele Name transgene insertion 254, Robert J Coffey
Mutation Made By Robert Coffey,   Vanderbilt University Medical Center
Strain of Origin(C57BL/6 x DBA/2)F2
Expressed Gene TGFA, transforming growth factor, alpha, human
Promoter MMTV, Mouse Mammary Tumor Virus, MMTV
Molecular Note The transgene contains the mouse mammary tumor virus LTR and the normal allele of the human transforming growth factor alpha gene. The vector also contains exons 2 and 3 of the rabbit beta-globin gene. Lines 254, 257, 64 and 29 were produced. [MGI Ref ID J:28453]

Control Information

  Control
   Noncarrier
   100006 B6D2F1/J (approximate)
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(MMTVTGFA)

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, this strain is maintained by mating a (C57BL/6J x DBA/2)F1 female with transgenic carrier male. Female transgenic mice do not lactate.

Related Strains

Strains carrying   Tg(MMTVTGFA)254Rjc allele
002953   FVB.Cg-Tg(MMTVTGFA)254Rjc/J
View Strains carrying   Tg(MMTVTGFA)254Rjc     (1 strain)

Strains carrying other alleles of TGFA
002373   B6D2-Tg(MMTVTGFA)29Rjc/J
002421   FVB/N-Tg(MtTGFA)100Lmb/J
View Strains carrying other alleles of TGFA     (2 strains)

View Strains carrying other alleles of MMTV     (16 strains)

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence (Mammary Gland Tumors)

References

Selected Reference(s)

Matsui Y; Halter SA; Holt JT; Hogan BL; Coffey RJ. 1990. Development of mammary hyperplasia and neoplasia in MMTV-TGF alpha transgenic mice. Cell 61(6):1147-55. [PubMed: 2161707]  [MGI Ref ID J:28453]

Additional References

Price and Supply Information

Strain Name: B6D2-Tg(MMTVTGFA)254Rjc/J
Stock Number: 002459

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

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Control InformationView Control Information in Strain Details.

General Terms and Conditions

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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