Strain Name: |
B6D2-Tg(MMTVTGFA)254Rjc/J |
|---|---|
Stock Number: |
002459 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | TGFA; MMTV; Tg(MMTVTGFA)254Rjc; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Robert Coffey, Vanderbilt University Medical Center Generation N?+2pN1 Strain Description
The phenotype of TgN(MMTVTGFA)254Rjc transgenic mice is very similar to that of B6D2-TgN(MMTVTGFA)29Rjc (Stock No. 002373). Mice are viable and fertile, but lactate insufficiently to support the litter. Virgin transgenic mice show no mammary gland abnormalities prepubertally, but adult virgin mice have considerable alveolar gland hyperplasia. Pregnant transgenic mice show marked proliferation of the stromal cells, and alveolar secretion is markedly increased compared to nontransgenic mice. After multiple pregnancies, isolated adenocarcinomas develop. There is no apparent phenotypic effect in males. In females transgene expression was localized to the small ducts and alveoli in both virgin and pregnant mice as evidenced by in situ hybridization and by immunohistochemistry. Immunostaining also revealed some stromal staining in the hyperplastic areas. Egfr mRNA expression was also increased in mammary tissues expressing high levels of the transgene. Crosses between TGFA transgenic mice and TGFB1 transgenic mice demonstrate that these growth factors oppose each other's action on mammary gland development. The B6D2-TgN(MMTVTGFA)254Rjc line is notable for sebaceous gland hyperplasia, which occurs in both male and female mice. The hyperplasia was evidenced as multiple raised, white plaques and nodules on the abdominal skin. No dysplasia or sebaceous cell carcinoma was observed. High expression of TGFa mRNA and TGFa protein was detected in the areas of sebaceous hyperplasia.Strain Development
To construct pMMTV-TGFa, a pKCR vector in which the SV40 early promoter region was replaced with complete MMTV LTR and a 925 bp human TGFa cDNA was inserted in the EcoRI site of b-globin exon 3. The 3.6 kb XhoI fragment was microinjected into (C57BL X DBA)F2 fertilized eggs. Transgenic mice are identified either by Southern blot using a b-globin exon 3 probe or by PCR. Transgenic lines were generated by mating founder animals to (C57BL X DBA/2)F1 males and females. No details of subsequent strain development were provided.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(MMTVTGFA)254Rjc | ||
|---|---|---|---|
| Allele Name | transgene insertion 254, Robert J Coffey | ||
| Mutation Made By | Robert Coffey, Vanderbilt University Medical Center | ||
| Strain of Origin | (C57BL/6 x DBA/2)F2 | ||
| Expressed Gene | TGFA, transforming growth factor, alpha, human | ||
| Promoter | MMTV, Mouse Mammary Tumor Virus, MMTV | ||
| Molecular Note | The transgene contains the mouse mammary tumor virus LTR and the normal allele of the human transforming growth factor alpha gene. The vector also contains exons 2 and 3 of the rabbit beta-globin gene. Lines 254, 257, 64 and 29 were produced. [MGI Ref ID J:28453] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100006 B6D2F1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(MMTVTGFA)
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, this strain is maintained by mating a (C57BL/6J x DBA/2)F1 female with transgenic carrier male. Female transgenic mice do not lactate. |
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Related Strains
Strains carrying Tg(MMTVTGFA)254Rjc allele
002953 FVB.Cg-Tg(MMTVTGFA)254Rjc/J View Strains carrying Tg(MMTVTGFA)254Rjc (1 strain)
002373 B6D2-Tg(MMTVTGFA)29Rjc/J 002421 FVB/N-Tg(MtTGFA)100Lmb/J
004997 B6.Cg-Tg(MMTV-TGFBR2)7Hlm/J 002618 B6.Cg-Tg(MMTVtTA)1Mam/J 007962 B6.FVB-Tg(MMTV-neu/OT-I/OT-II)CBnel Tg(Trp53R172H)8512Jmr/J 002375 B6;D2-Tg(MMTVTGFB1)46Hlm/J 002373 B6D2-Tg(MMTVTGFA)29Rjc/J 002870 B6SJL-Tg(Wnt1)1Hev/J 005038 FVB-Tg(MMTV-Erbb2)NK1Mul/J 004363 FVB.Cg-Tg(MMTV-vHaras)SH1Led/J 002934 FVB.Cg-Tg(Wnt1)1Hev/J 002437 FVB/N-Tg(MMTV-Notch4)3Rnc/J 002374 FVB/N-Tg(MMTV-PyVT)634Mul/J 002376 FVB/N-Tg(MMTVneu)202Mul/J 002933 FVB/NJ-Tg(MMTVTGFB1)46Hlm/J 003690 STOCK Tg(MMTV-Cdc37)1Stp/J 003551 STOCK Tg(MMTV-cre)1Mam/J 003553 STOCK Tg(MMTV-cre)4Mam/J
Research Applications
This mouse can be used to support research in many areas including:
Cancer Research
Increased Tumor Incidence (Mammary Gland Tumors)
References
Selected Reference(s)
Additional ReferencesMatsui Y; Halter SA; Holt JT; Hogan BL; Coffey RJ. 1990. Development of mammary hyperplasia and neoplasia in MMTV-TGF alpha transgenic mice. Cell 61(6):1147-55. [PubMed: 2161707] [MGI Ref ID J:28453]
Price and Supply Information
| Strain Name: | B6D2-Tg(MMTVTGFA)254Rjc/J |
| Stock Number: | 002459 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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