Strain Name:

B6D2-Tg(MMTVTGFA)254Rjc/J

Stock Number:

002459

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
GenerationN?+2pN1
 
Donating Investigator Robert Coffey,   Vanderbilt University Medical Center

Description
The phenotype of TgN(MMTVTGFA)254Rjc transgenic mice is very similar to that of B6D2-TgN(MMTVTGFA)29Rjc (Stock No. 002373). Mice are viable and fertile, but lactate insufficiently to support the litter. Virgin transgenic mice show no mammary gland abnormalities prepubertally, but adult virgin mice have considerable alveolar gland hyperplasia. Pregnant transgenic mice show marked proliferation of the stromal cells, and alveolar secretion is markedly increased compared to nontransgenic mice. After multiple pregnancies, isolated adenocarcinomas develop. There is no apparent phenotypic effect in males. In females transgene expression was localized to the small ducts and alveoli in both virgin and pregnant mice as evidenced by in situ hybridization and by immunohistochemistry. Immunostaining also revealed some stromal staining in the hyperplastic areas. Egfr mRNA expression was also increased in mammary tissues expressing high levels of the transgene. Crosses between TGFA transgenic mice and TGFB1 transgenic mice demonstrate that these growth factors oppose each other's action on mammary gland development. The B6D2-TgN(MMTVTGFA)254Rjc line is notable for sebaceous gland hyperplasia, which occurs in both male and female mice. The hyperplasia was evidenced as multiple raised, white plaques and nodules on the abdominal skin. No dysplasia or sebaceous cell carcinoma was observed. High expression of TGFa mRNA and TGFa protein was detected in the areas of sebaceous hyperplasia.

Development
To construct pMMTV-TGFa, a pKCR vector in which the SV40 early promoter region was replaced with complete MMTV LTR and a 925 bp human TGFa cDNA was inserted in the EcoRI site of b-globin exon 3. The 3.6 kb XhoI fragment was microinjected into (C57BL X DBA)F2 fertilized eggs. Transgenic mice are identified either by Southern blot using a b-globin exon 3 probe or by PCR. Transgenic lines were generated by mating founder animals to (C57BL X DBA/2)F1 males and females. No details of subsequent strain development were provided.

Control Information

  Control
   Noncarrier
   100006 B6D2F1/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Tg(MMTVTGFA)254Rjc allele
002953   FVB.Cg-Tg(MMTVTGFA)254Rjc/J
View Strains carrying   Tg(MMTVTGFA)254Rjc     (1 strain)

Strains carrying other alleles of TGFA
002373   B6D2-Tg(MMTVTGFA)29Rjc/J
002421   FVB/N-Tg(MtTGFA)100Lmb/J
002422   STOCK Tg(MtTGFA)42Lmb/J
003722   SW.Cg-Tg(MtTGFA)42Lmb/J
View Strains carrying other alleles of TGFA     (4 strains)

View Strains carrying other alleles of MMTV     (18 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Tg(MMTVTGFA)254Rjc/0

        involves: C57BL/6 * DBA/2
  • endocrine/exocrine gland phenotype
  • abnormal mammary gland morphology (MGI Ref ID J:28453)
    • at 2.5 months of age, the virgin female founder displayed duct abnormalities but little alveolar development
    • mammary gland hyperplasia (MGI Ref ID J:72085)
      • female mice develop cystic and solid hyperplasias
  • enlarged sebaceous gland (MGI Ref ID J:72085)
    • mice exhibit sebaceous gland hyperplasia
  • tumorigenesis
  • mammary adenocarcinoma (MGI Ref ID J:72085)
  • behavior/neurological phenotype
  • abnormal nursing (MGI Ref ID J:72085)
    • females cannot nurse her young
  • skin/coat/nails phenotype
  • abnormal skin condition (MGI Ref ID J:72085)
    • mice exhibit multiple, raised, white plaques and nodules on the abdominal skin
  • reproductive system phenotype
  • abnormal mammary gland morphology (MGI Ref ID J:28453)
    • at 2.5 months of age, the virgin female founder displayed duct abnormalities but little alveolar development
    • mammary gland hyperplasia (MGI Ref ID J:72085)
      • female mice develop cystic and solid hyperplasias
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence
      Mammary Gland Tumors

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Tg(MMTVTGFA)254Rjc
Allele Name transgene insertion 254, Robert J Coffey
Allele Type Transgenic (random, expressed)
Mutation Made By Robert Coffey,   Vanderbilt University Medical Center
Strain of Origin(C57BL/6 x DBA/2)F2
Expressed Gene TGFA, transforming growth factor, alpha, human
Promoter MMTV, Mouse Mammary Tumor Virus, MMTV
Molecular Note The transgene contains the mouse mammary tumor virus LTR and the normal allele of the human transforming growth factor alpha gene. The vector also contains exons 2 and 3 of the rabbit beta-globin gene. Lines 254, 257, 64 and 29 were produced. [MGI Ref ID J:28453]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(MMTVTGFA), Standard PCR
Tg(MMTVTGFA), Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Matsui Y; Halter SA; Holt JT; Hogan BL; Coffey RJ. 1990. Development of mammary hyperplasia and neoplasia in MMTV-TGF alpha transgenic mice. Cell 61(6):1147-55. [PubMed: 2161707]  [MGI Ref ID J:28453]

Additional References

Halter SA; Dempsey P; Matsui Y; Stokes MK; Graves-Deal R; Hogan BL; Coffey RJ. 1992. Distinctive patterns of hyperplasia in transgenic mice with mouse mammary tumor virus transforming growth factor-alpha. Characterization of mammary gland and skin proliferations. Am J Pathol 140(5):1131-46. [PubMed: 1316084]  [MGI Ref ID J:72085]

Pierce DF Jr; Gorska AE; Chytil A; Meise KS; Page DL; Coffey RJ Jr; Moses HL. 1995. Mammary tumor suppression by transforming growth factor beta 1 transgene expression. Proc Natl Acad Sci U S A 92(10):4254-8. [PubMed: 7753792]  [MGI Ref ID J:71696]

Tg(MMTVTGFA)254Rjc related

Cleary MP; Grande JP; Maihle NJ. 2004. Effect of high fat diet on body weight and mammary tumor latency in MMTV-TGF-alpha mice. Int J Obes Relat Metab Disord 28(8):956-62. [PubMed: 15254485]  [MGI Ref ID J:92145]

Cleary MP; Hu X; Grossmann ME; Juneja SC; Dogan S; Grande JP; Maihle NJ. 2007. Prevention of mammary tumorigenesis by intermittent caloric restriction: does caloric intake during refeeding modulate the response? Exp Biol Med (Maywood) 232(1):70-80. [PubMed: 17202587]  [MGI Ref ID J:129353]

Cleary MP; Jacobson MK; Phillips FC; Getzin SC; Grande JP; Maihle NJ. 2002. Weight-cycling decreases incidence and increases latency of mammary tumors to a greater extent than does chronic caloric restriction in mouse mammary tumor virus-transforming growth factor-alpha female mice. Cancer Epidemiol Biomarkers Prev 11(9):836-43. [PubMed: 12223427]  [MGI Ref ID J:79070]

Cleary MP; Juneja SC; Phillips FC; Hu X; Grande JP; Maihle NJ. 2004. Leptin receptor-deficient MMTV-TGF-alpha/Lepr(db)Lepr(db) female mice do not develop oncogene-induced mammary tumors. Exp Biol Med (Maywood) 229(2):182-93. [PubMed: 14734797]  [MGI Ref ID J:102011]

Cleary MP; Phillips FC; Getzin SC; Jacobson TL; Jacobson MK; Christensen TA; Juneja SC; Grande JP; Maihle NJ. 2003. Genetically obese MMTV-TGF-alpha/Lep(ob)Lep(ob) female mice do not develop mammary tumors. Breast Cancer Res Treat 77(3):205-15. [PubMed: 12602920]  [MGI Ref ID J:81933]

Dogan S; Hu X; Zhang Y; Maihle NJ; Grande JP; Cleary MP. 2007. Effects of high-fat diet and/or body weight on mammary tumor leptin and apoptosis signaling pathways in MMTV-TGF-alpha mice. Breast Cancer Res 9(6):R91. [PubMed: 18162139]  [MGI Ref ID J:133411]

Halter SA; Dempsey P; Matsui Y; Stokes MK; Graves-Deal R; Hogan BL; Coffey RJ. 1992. Distinctive patterns of hyperplasia in transgenic mice with mouse mammary tumor virus transforming growth factor-alpha. Characterization of mammary gland and skin proliferations. Am J Pathol 140(5):1131-46. [PubMed: 1316084]  [MGI Ref ID J:72085]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, this strain is maintained by mating a (C57BL/6J x DBA/2)F1 female with transgenic carrier male. Female transgenic mice do not lactate.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Price (US dollars $)
Cryorecovery Fee $1900.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Price (US dollars $)
Cryorecovery Fee $2470.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Supply Details

Standard SupplyCryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   Noncarrier
   100006 B6D2F1/J (approximate)
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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