Strain Name:

B6.129S-Itga4tm1Hyn/J

Stock Number:

002463

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Availability:

Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain 129S2 via D3 ES cell line
 
Donating InvestigatorDr. Richard Hynes,   Massachusetts Institute of Technology

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Itga4tm1Hyn targeted mutation die during embryonic development. Homozygous mutant embryos fail to fuse the allantois with the chorion during placentation. There is a defect in the epicardium and coronary vessels results in in utero cardiac hemorrhage; also known as CD49D, VLA-4.

Development
The Itga4-deficient strain was developed in the lab of Dr. Richard O. Hynes at the Center for Cancer Research at the Massachusetts Institute of Technology.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Itga4
010501   C57BL/6-Itga4tm1Mshi/J
View Strains carrying other alleles of Itga4     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Itga4tm1Hyn/Itga4tm1Hyn

        either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6J)
  • mortality/aging
  • partial embryonic lethality during organogenesis
    • about 50% die at around E11 with failure of allantois-chorion fusion   (MGI Ref ID J:23140)
    • about 50% die around E12.5 with cardiac defects, although a few survive up to E14.5   (MGI Ref ID J:23140)
  • embryogenesis phenotype
  • abnormal allantois morphology
    • about half of the embryos exhibit allantois defects between E9.5 and E11.5   (MGI Ref ID J:23140)
    • dilated allantois
      • allantois has an abnormal balloon shape   (MGI Ref ID J:23140)
  • abnormal placenta morphology
    • placenta lacks the allantoic component at E9.5 and as a result, the placenta does not form a connection with the embryo   (MGI Ref ID J:23140)
    • failure of chorioallantoic fusion
      • failure of fusion of the allantois with the chorion   (MGI Ref ID J:23140)
  • cardiovascular system phenotype
  • abnormal cardiovascular system morphology
    • the 50% of embryos that survive past E11.5 develop cardiac abnormalities between E11.5 and E14.5 but do not have allantois defects   (MGI Ref ID J:23140)
    • abnormal coronary vessel morphology
      • coronary vessels at the atrioventricular sulcus region are absent   (MGI Ref ID J:23140)
    • abnormal epicardium morphology
      • epicardium is missing on the entire ventricular surface and on part of the atrial surface at E11.5, but not at E10.5   (MGI Ref ID J:23140)
  • heart hemorrhage
    • cardiac hemorrhage   (MGI Ref ID J:23140)
  • craniofacial phenotype
  • abnormal craniofacial morphology
    • embryos with cardiac defects exhibit abnormalities in the cranial and facial structures   (MGI Ref ID J:23140)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Itga4tm1Hyn related

Cardiovascular Research
Heart Abnormalities
Vascular Defects

Cell Biology Research
Cell Motility Defects
Defects in Cell Adhesion Molecules

Developmental Biology Research
Cell Motility Defects
Defects in Cell Adhesion Molecules
Defects in Extracellular Matrix Molecules
Embryonic Lethality (Homozygous)
Internal/Organ Defects
      heart
Neural Crest Defects

Hematological Research
Hematopoietic Defects
Immunological Defects

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
      B cell deficiency
Immunodeficiency Associated with Other Defects
Inflammation
Lymphoid Tissue Defects

Internal/Organ Research
Heart Abnormalities
X Placental Development

Research Tools
Cell Biology Research
Developmental Biology Research
Immunology, Inflammation and Autoimmunity Research
Neurobiology Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Itga4tm1Hyn
Allele Name targeted mutation 1, Richard Hynes
Allele Type Targeted (Null/Knockout)
Common Name(s) alpha4-null; alpha4KO;
Mutation Made ByDr. Richard Hynes,   Massachusetts Institute of Technology
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name Itga4, integrin alpha 4
Chromosome 2
Gene Common Name(s) CD49D; IA4; VLA-4 receptor, alpha 4 subunit;
Molecular Note A neomycin resistance cassette replaced a 950 bp fragment which contained most of the first exon of the gene. [MGI Ref ID J:23140]

Genotyping

Genotyping Information

Genotyping Protocols

Itga4tm1Hyn, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Yang JT; Rayburn H; Hynes RO. 1995. Cell adhesion events mediated by alpha 4 integrins are essential in placental and cardiac development. Development 121(2):549-60. [PubMed: 7539359]  [MGI Ref ID J:23140]

Additional References

Yang JT; Rando TA; Mohler WA; Rayburn H; Blau HM; Hynes RO. 1996. Genetic analysis of alpha 4 integrin functions in the development of mouse skeletal muscle. J Cell Biol 135(3):829-35. [PubMed: 8909554]  [MGI Ref ID J:36475]

Itga4tm1Hyn related

Arroyo AG; Yang JT; Rayburn H; Hynes RO. 1999. Alpha4 integrins regulate the proliferation/differentiation balance of multilineage hematopoietic progenitors in vivo. Immunity 11(5):555-66. [PubMed: 10591181]  [MGI Ref ID J:58741]

Grazioli A; Alves CS; Konstantopoulos K; Yang JT. 2006. Defective blood vessel development and pericyte/pvSMC distribution in alpha4 integrin-deficient mouse embryos. Dev Biol 293(1):165-77. [PubMed: 16529735]  [MGI Ref ID J:108464]

Gribi R; Hook L; Ure J; Medvinsky A. 2006. The differentiation program of embryonic definitive hematopoietic stem cells is largely alpha4 integrin independent. Blood 108(2):501-9. [PubMed: 16551970]  [MGI Ref ID J:135680]

Haack H; Hynes RO. 2001. Integrin receptors are required for cell survival and proliferation during development of the peripheral glial lineage. Dev Biol 233(1):38-55. [PubMed: 11319856]  [MGI Ref ID J:69286]

Sengbusch JK; He W; Pinco KA; Yang JT. 2002. Dual functions of [alpha]4[beta]1 integrin in epicardial development: initial migration and long-term attachment. J Cell Biol 157(5):873-82. [PubMed: 12021259]  [MGI Ref ID J:76747]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThe strain is currently begin maintained by heterozygous matings. Heterozygotes are good breeders. Progeny require testing to determine genotype. The strain was generated on a mixed 129/Sv x C57BL/6 background and is now on both C57BL/6 and 129/Sv backgrounds. Expected coat color from breeding:Black

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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