Go to JAX® Mice Query Form

Strain Name:

B6;129S7-Lrpap1tm1Her/J

Stock Number:

002464

Availability:

Repository-Cryopreserved

View Pricing for USA, Canada and Mexico
View Pricing for International

General Terms and Conditions

Genes & Alleles   Lrpap1;   Lrpap1tm1Her;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Targeted Mutation
Specieslaboratory mouse
Donating Investigator Joachim Herz,   Univ of Texas Southwest Med Ctr Dallas
Generation+N1

Appearance
black
Related Genotype: a/a

Strain Description
Mice homozygous for the Lrpap1tm1Her targeted mutation (commonly referred to as RAP) are viable and fertile. They have a functional deficiency of the low density lipoprotein receptor-related protein (LRP) with reduced amounts of LRP seen in both the liver and the brain of homozygotes.

Mammalian Phenotype Terms assigned by genotype

Lrpap1tm1Her/Lrpap1tm1Her

        involves: 129S7/SvEvBrd * C57BL/6J
  • homeostasis/metabolism phenotype
  • abnormal circulating protein level (MGI Ref ID J:43736)
    • alpha2 macroglobulin is removed from the circulation at about 25% of the normal rate
    • no effect is seen on blood lipoproteins regardless of diet
    • homozygotes thrived well and are superficially normal
  • other phenotype
  • other aberrant phenotype (MGI Ref ID J:43736)
    • reduces expression of lipoprotein receptor related protein by 75% but has no effect on the LDL receptor

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Lrpap1tm1Her/Lrpap1tm1Her

        B6.129S7-Lrpap1tm1Her
  • renal/urinary system phenotype
  • abnormal kidney physiology (MGI Ref ID J:106203)
    • endocytotic machinery in the proximal tubule is disturbed athough the ability to adjust to dietary phosphate levels is unaffected
  • endocrine/exocrine gland phenotype
  • abnormal thyroid gland morphology (MGI Ref ID J:107358)
    • slight thyrocyte and nuclear hypertrophy but no goiter
  • abnormal thyroid physiology (MGI Ref ID J:107358)
    • slightly reduced thyroglobulin in thyroid follicles but intracellular levels and levels on the apical membrane of thyrocytes are increased
    • T4 levels in thyroglobulin are normal or slightly elevated
    • free T4 levels unchanged
    • TSH levels are elevated but not to the point of statistical significance
  • nervous system phenotype
  • abnormal brain interneuron morphology (MGI Ref ID J:107552)
    • levels of somatostatin in neurons reduced
    • number of neurons expressing somatostatin is reduced
  • behavior/neurological phenotype
  • abnormal spatial learning (MGI Ref ID J:107552)
    • poorer performance in a Morris water maze
    • after 6 days of training, swim a greater distance to find target than do controls

Gene & Allele Details

Allele Symbol Lrpap1tm1Her
Allele Name targeted mutation 1, Joachim Herz
Common Name(s) RAP-;
Mutation Made By Joachim Herz,   Univ of Texas Southwest Med Ctr Dallas
Strain of Origin129S7/SvEvBrd
ES Cell Line NameOther (see notes)
ES Cell Line Strain129
Gene Symbol and Name Lrpap1, low density lipoprotein receptor-related protein associated protein 1
Chromosome 5
Gene Common Name(s) A2MRAP; A2RAP; AA617339; AI790446; AU042172; C77774; HBP44; MGC138272; MRAP; RAP; expressed sequence AA617339; expressed sequence AI790446; expressed sequence AU042172; expressed sequence C77774;
General Note ES cell line = AB1 (129S7/SvEvBrd-Hprt+) or JH1 (129S7/SvEvBrd).
Molecular Note A neomycin resistance cassette was inserted into sequences that encode the leader peptide. Immunoblot analysis of liver and brain did not detect the encoded protein in homozygous animals. [MGI Ref ID J:43736]

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Genotyping Protocols

Lrpaptm1Her

Colony Maintenance

Breeding & HusbandryThe strain is maintained by homozygous siblings mating . Expected coat color from breeding:Black
Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying   Lrpap1tm1Her allele
002987   B6.129S7-Lrpap1tm1Her/J
002465   B6;129S-Ldlrtm1Her Lrpap1tm1Her/J
View Strains carrying   Lrpap1tm1Her     (2 strains)

Research Applications

This mouse can be used to support research in many areas including:

Lrpap1tm1Her related

Cardiovascular Research
Other (altered lipoprotein profile)

References

Selected Reference(s)

Willnow TE; Armstrong SA; Hammer RE; Herz J. 1995. Functional expression of low density lipoprotein receptor-related protein is controlled by receptor-associated protein in vivo. Proc Natl Acad Sci U S A 92(10):4537-41. [PubMed: 7538675]  [MGI Ref ID J:43736]

Additional References

Price and Supply Information

Strain Name: B6;129S7-Lrpap1tm1Her/J
Stock Number: 002464

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.

*NO Shipping Destination selected!

 

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

      Purchasing Information
      JAX® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Go to JAX® Mice Query Form

(2.15)