Strain Name: |
B6;129S-Ldlrtm1Her Lrpap1tm1Her/J |
|---|---|
Stock Number: |
002465 |
Availability: | Repository-Cryopreserved |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Ldlr; Ldlrtm1Her; Lrpap1; Lrpap1tm1Her; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Joachim Herz, Univ of Texas Southwest Med Ctr Dallas Generation F9 Strain Description
Mice homozygous for both the Ldlrtm1Her and Lrpap1tm1Her targeted mutations are viable and fertile. They have a functional deficiency of the low density lipoprotein receptor-related protein (LRP) and low density lipoprotein receptor (LDLR). LRP is reduced in both the liver and the brain of homozygotes. They also show impaired hepatic clearance of alpha2-macroglobulin and plasma accumulation of remnant lipoproteins.Strain Development
This strain was developed in the lab of Dr. Joachim Herz at The University of Texas, Southwestern Medical Center at Dallas by mating Lrpap1-deficient and Ldlr-deficient mice.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Ldlrtm1Her | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Joachim Herz | ||
| Common Name(s) | LDLR KO; LDLR-; LDLr0; LDLrKO; Ldlrtm1Her; | ||
| Mutation Made By | Joachim Herz, Univ of Texas Southwest Med Ctr Dallas | ||
| Strain of Origin | 129S7/SvEvBrd-Hprt1<+> | ||
| ES Cell Line Name | AB1 | ||
| ES Cell Line Strain | 129S7/SvEvBrd-Hprt1<+> | ||
| Gene Symbol and Name | Ldlr, low density lipoprotein receptor | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | FH; FHC; LDLRA; | ||
| General Note | When used in bone marrow transplant into Ldlrtm1Her homozygous mice, Abca1tm1Jdm Abcg1tm1Dgen homozygous cells accelerate the development of atherosclerosis. (J:130777) | ||
| Molecular Note | Insertion of a neomycin resistance cassette into exon 4. The authors predict that the targeted allele would encode a truncated non-functional protein that will not bind LDL, and that lacks a membrane spanning segment. Immunoblot analysis of liver membranes detected a truncated protein in homozygous mutant animals. [MGI Ref ID J:37394] | ||
| Allele Symbol | Lrpap1tm1Her | ||
| Allele Name | targeted mutation 1, Joachim Herz | ||
| Common Name(s) | RAP-; | ||
| Mutation Made By | Joachim Herz, Univ of Texas Southwest Med Ctr Dallas | ||
| Strain of Origin | 129S7/SvEvBrd | ||
| ES Cell Line Name | Other (see notes) | ||
| ES Cell Line Strain | 129 | ||
| Gene Symbol and Name | Lrpap1, low density lipoprotein receptor-related protein associated protein 1 | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | A2MRAP; A2RAP; AA617339; AI790446; AU042172; C77774; HBP44; MGC138272; MRAP; RAP; expressed sequence AA617339; expressed sequence AI790446; expressed sequence AU042172; expressed sequence C77774; | ||
| General Note | ES cell line = AB1 (129S7/SvEvBrd-Hprt+) or JH1 (129S7/SvEvBrd). | ||
| Molecular Note | A neomycin resistance cassette was inserted into sequences that encode the leader peptide. Immunoblot analysis of liver and brain did not detect the encoded protein in homozygous animals. [MGI Ref ID J:43736] | ||
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Ldlr tm1Her
Lrpaptm1Her
Colony Maintenance
| Breeding & Husbandry | The strain is maintained by mating homozygous siblings. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Ldlrtm1Her allele
002246 B6.129-Apoetm1Unc Ldlrtm1Her/J 002207 B6.129S7-Ldlrtm1Her/J 006952 B6.Cg-Akt2tm1.1Mbb Ldlrtm1Her/J 006580 B6.Cg-Ins2Akita Ldlrtm1Her/J 006883 B6.Cg-Ldlrtm1Her Sod2tm1Leb/J 006877 B6.Cg-Ldlrtm1Her Tg(H2-K-AKR1B1)1Tj/J 006906 B6.Cg-Lepob Ldlrtm1Her/J 002245 B6;129-Apoetm1Unc Ldlrtm1Her/J 003000 B6;129S-Apobtm2Sgy Ldlrtm1Her/J 002077 B6;129S7-Ldlrtm1Her/J 004192 STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy Tg(Mx1-cre)1Cgn/J View Strains carrying Ldlrtm1Her (11 strains)
002987 B6.129S7-Lrpap1tm1Her/J 002464 B6;129S7-Lrpap1tm1Her/J
005061 C57BL/6J-LdlrHlb301/J
Research Applications
This mouse can be used to support research in many areas including:Ldlrtm1Her related
Lrpap1tm1Her relatedCardiovascular Research
Atherosclerosis
Hypercholesterolemia
Metabolism Research
Lipid Metabolism
Mouse/Human Gene Homologs
hypercholesterolemia, familial
Cardiovascular Research
Other (altered lipoprotein profile)
References
Selected Reference(s)
Additional ReferencesWillnow TE; Armstrong SA; Hammer RE; Herz J. 1995. Functional expression of low density lipoprotein receptor-related protein is controlled by receptor-associated protein in vivo. Proc Natl Acad Sci U S A 92(10):4537-41. [PubMed: 7538675] [MGI Ref ID J:43736]
Price and Supply Information
| Strain Name: | B6;129S-Ldlrtm1Her Lrpap1tm1Her/J |
| Stock Number: | 002465 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | |||||
|---|---|---|---|---|---|
| Cryorecovery Fee | $1900.00 | ||||
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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