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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names STOCK TgN(hCMV-Cre)140Sau (Changed: 15-DEC-04 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation F?+3p Donating Investigator Brian Sauer, Stowers Institute for Medical Research Description
This strain contains a Cre recombinase gene under the control of a human cytomegalovirus minimal promoter. This promoter directs transcription such that Cre recombinase is expressed in all tissues.Development
These were made at DuPont in 1988 by pronuclear injection of zygotes from a cross of female C57BL/6NCrlBR x male DBA/2NCrlBR (ie hybrid BDF1 from Charles River). Male and female F1 progeny from the original founder were intercrossed for several generations, and a hemizygous transgenic was then outcrossed with FVB/N mice for several (~3) generations to produce a homozygous transgenic line. The original founder was Tg(O-Eco-Cre)DuPG140, founder #140. It carries the hCMV-cre expression cassette on a HindIII fragment from plasmid pBS185 (Sauer and Henderson, 1990)
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (125 strains)
006054 B6.C-Tg(CMV-cre)1Cgn/J 003465 BALB/c-Tg(CMV-cre)1Cgn/J 006362 C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J 006439 FVB-Tg(tetO/CMV-KRAS*G12C)9.1Msmi/J 004375 FVB/N-Tg(tetO-Kras2)12Hev/J
Additional Web Information
Cre-lox Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre Recombinase Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(hCMV-cre)140Sau | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Brian Sauer | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | hCMV-Cre; | ||
| Mutation Made By | Brian Sauer, Stowers Institute for Medical Research | ||
| Strain of Origin | C57BL/6NCrlBR x DBA/2NCrlBR | ||
| Site of Expression | widespread expression, including germline | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | CMV, cytomegalovirus, human | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a human cytomegalovirus promoter/enhancer, active in most cells and tissues. [MGI Ref ID J:75205] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Lakso M; Sauer B; Mosinger B Jr; Lee EJ; Manning RW; Yu SH; Mulder KL; Westphal H. 1992. Targeted oncogene activation by site-specific recombination in transgenic mice. Proc Natl Acad Sci U S A 89(14):6232-6. [PubMed: 1631115] [MGI Ref ID J:75205]
Additional References
Oravecz-Wilson KI; Kiel MJ; Li L; Rao DS; Saint-Dic D; Kumar PD; Provot MM; Hankenson KD; Reddy VN; Lieberman AP; Morrison SJ; Ross TS. 2004. Huntingtin Interacting Protein 1 mutations lead to abnormal hematopoiesis, spinal defects and cataracts. Hum Mol Genet 13(8):851-67. [PubMed: 14998932] [MGI Ref ID J:90400]
Tg(hCMV-cre)140Sau relatedCohen T; Auerbach W; Ravid L; Bodennec J; Fein A; Futerman AH; Joyner AL; Horowitz M. 2005. The exon 8-containing prosaposin gene splice variant is dispensable for mouse development, lysosomal function, and secretion. Mol Cell Biol 25(6):2431-40. [PubMed: 15743835] [MGI Ref ID J:97577]
Huang J; Cheng L; Li J; Chen M; Zhou D; Lu MM; Proweller A; Epstein JA; Parmacek MS. 2008. Myocardin regulates expression of contractile genes in smooth muscle cells and is required for closure of the ductus arteriosus in mice. J Clin Invest 118(2):515-25. [PubMed: 18188448] [MGI Ref ID J:131288]
Ippolito GC; Schelonka RL; Zemlin M; Ivanov II; Kobayashi R; Zemlin C; Gartland GL; Nitschke L; Pelkonen J; Fujihashi K; Rajewsky K; Schroeder HW Jr. 2006. Forced usage of positively charged amino acids in immunoglobulin CDR-H3 impairs B cell development and antibody production. J Exp Med 203(6):1567-78. [PubMed: 16754718] [MGI Ref ID J:124421]
Keel SB; Doty RT; Yang Z; Quigley JG; Chen J; Knoblaugh S; Kingsley PD; De Domenico I; Vaughn MB; Kaplan J; Palis J; Abkowitz JL. 2008. A heme export protein is required for red blood cell differentiation and iron homeostasis. Science 319(5864):825-8. [PubMed: 18258918] [MGI Ref ID J:132057]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry Expected coat color from breeding:Albino Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact Information
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Tel: 800.422.6423 or 207.288.5845
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Terms of Use
Terms of Use
General Terms and Conditions
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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