Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N?+3F5pN1 (15-JAN-06)   Donating Investigator Lionel Larue,   Max Planck Institute for Immunobiology

Description
Mice homozygous for this targeted mutation tend to die at the time of implantation. Preimplantation embryos immunostain positive for the endogenous gene product and undergo compaction, but this results not from endogenous expression, but from residual maternal Cdh-1. By embryonic day 4.5 the homozygous null embryos exhibit greatly diminished cell-cell contact and fail to form a trophectodermal epithelium or a blastocyst cavity. Heterozygous mutant mice are viable and fertile with no grossly observable defects.

Development
This targeted mutation was developed in the lab of Dr. Lionel Larue at the Max Planck Institute for Immunobiology. A targeting vector designed to insert a lacZ-poly-A and neo^r between the transcriptional and translational start sites; thus the construct is under the control of the endogenous promoter. This also placed a mRNA destabilizing sequence after exon 2. The construct was electroporated into 129S2/SvPas-derived D3 ES cell line was used. Correctly targeted ES cells were injected in C57BL/6 blastocysts and coat color chimeras were bred. Heterozygotes were intercrossed to maintain the line.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Cdh1

005319

B6.129-Cdh1tm2Kem/J

View Strains carrying other alleles of Cdh1     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Cdh1^tm1Kem/Cdh1^tm1Kem

        involves: 129S2/SvPas * C57BL/6

• lethality-prenatal/perinatal
• embryonic lethality before implantation (MGI Ref ID J:19904)
  • death between E3.5-E4.5
• embryogenesis phenotype
• abnormal embryonic tissue morphology (MGI Ref ID J:19904)
• abnormal trophectoderm morphology (MGI Ref ID J:19904)
  • failure to form trophectodermal epithelium
• failure to form blastocele (MGI Ref ID J:19904)
  • failure to form blastocyst cavity
• abnormal extraembryonic tissue morphology (MGI Ref ID J:19904)
• abnormal trophectoderm morphology (MGI Ref ID J:19904)
  • failure to form trophectodermal epithelium

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cdh1^tm1Kem related

Cell Biology Research
Defects in Cell Adhesion Molecules

Developmental Biology Research
Defects in Cell Adhesion Molecules
Embryonic Lethality (Homozygous)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Cdh1tm1Kem
Allele Name		targeted mutation 1, Rolf Kemler
Allele Type		Targeted (Reporter)
Common Name(s)		E-cad-;
Mutation Made By		Lionel Larue,   Max Planck Institute for Immunobiology
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Cdh1, cadherin 1
Chromosome		8
Gene Common Name(s)		AA960649; Arc-1; CD324; CDHE; E-cadherin; ECAD; Ecad; L-CAM; LCAM; UM; UVO; Um; expressed sequence AA960649; uvomorulin;
Molecular Note		A lacZ gene and neomycin resistance cassette was inserted into exon 1 just upstream of the initiation codon of the gene. [MGI Ref ID J:19904]

Genotyping

Genotyping Information

Genotyping Protocols

Cdh1^tm1Kem, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Larue L; Ohsugi M; Hirchenhain J; Kemler R. 1994. E-cadherin null mutant embryos fail to form a trophectoderm epithelium. Proc Natl Acad Sci U S A 91(17):8263-7. [PubMed: 8058792]  [MGI Ref ID J:19904]

Additional References

Cdh1^tm1Kem related

Boussadia O; Kutsch S; Hierholzer A; Delmas V; Kemler R. 2002. E-cadherin is a survival factor for the lactating mouse mammary gland. Mech Dev 115(1-2):53-62. [PubMed: 12049767]  [MGI Ref ID J:77170]

Cali G; Zannini M; Rubini P; Tacchetti C; D'Andrea B; Affuso A; Wintermantel T; Boussadia O; Terracciano D; Silberschmidt D; Amendola E; De Felice M; Schutz G; Kemler R; Di Lauro R; Nitsch L. 2007. Conditional inactivation of the E-cadherin gene in thyroid follicular cells affects gland development but does not impair junction formation. Endocrinology 148(6):2737-46. [PubMed: 17347311]  [MGI Ref ID J:122323]

Larue L; Antos C; Butz S; Huber O; Delmas V; Dominis M; Kemler R. 1996. A role for cadherins in tissue formation. Development 122(10):3185-94. [PubMed: 8898231]  [MGI Ref ID J:36233]

Ohsugi M; Larue L; Schwarz H; Kemler R. 1997. Cell-junctional and cytoskeletal organization in mouse blastocysts lacking E-cadherin. Dev Biol 185(2):261-71. [PubMed: 9187087]  [MGI Ref ID J:40476]

Young P; Boussadia O; Halfter H; Grose R; Berger P; Leone DP; Robenek H; Charnay P; Kemler R; Suter U. 2003. E-cadherin controls adherens junctions in the epidermis and the renewal of hair follicles. EMBO J 22(21):5723-33. [PubMed: 14592971]  [MGI Ref ID J:86510]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation are embryonic lethal. Expected coat color from breeding:Black
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

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