Strain Name:

B6.129S1-Myf6tm1Wb/J

Stock Number:

002475

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Availability:

Cryopreserved - Ready for recovery

These Myf6 deficient mice have muscle and skeletal patterning abnormalities.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain 129S1 via CJ7 ES cell line
 
Donating InvestigatorDr. Barbara Wold,   California Institute of Technology

Description
Homozygotes have major defects in both pattern and myotomal formation.

Development
This strain was developed in the lab of Dr. Barbara Wold at the California Institute of Technology. Coding sequence of the myogenic factor 6 (Myf6) gene was replaced with a neomycin resistance cassette. This construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+-derived CJ7 embryonic Stem cells (ES cells). Correctly targeted ES cells was injected into blastocysts, and the resulting mice were bred to C57BL/6J inbred mice (Stock No. 000664).

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Myf6
010528   B6;129-Myf6tm2(cre)Mrc/J
View Strains carrying other alleles of Myf6     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Myopathy, Centronuclear, 3; CNM3   (MYF6)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Myf6tm1Wb/Myf6tm1Wb

        involves: 129S1/Sv * C57BL/6
  • mortality/aging
  • complete neonatal lethality
    • die shortly after birth   (MGI Ref ID J:29279)
  • respiratory system phenotype
  • respiratory distress
    • unable to breathe   (MGI Ref ID J:29279)
  • skeleton phenotype
  • abnormal cervical vertebrae morphology
    • the tuberculum anterior is absent on the 6th cervical vertebrae of most mice   (MGI Ref ID J:29279)
  • abnormal rib morphology
    • rib development is severely disturbed in newborns   (MGI Ref ID J:29279)
    • some ribs display abnormal angles of extension   (MGI Ref ID J:29279)
    • rib bifurcation   (MGI Ref ID J:29279)
    • rib fusion
      • fusion of rib cartilage from adjacent ribs   (MGI Ref ID J:29279)
    • short ribs
      • rib foreshortening in ribs 2-12 is usually less than 20% of total length and leads to variable failure to join the sternum   (MGI Ref ID J:29279)
      • the first and last ribs are more dramatically shortened compared to others   (MGI Ref ID J:29279)
    • truncated ribs
      • truncated ribs that fail to attach to the sternum   (MGI Ref ID J:29279)
  • abnormal rib-sternum attachment
    • rib foreshortening leads to variable failure to join the sternum   (MGI Ref ID J:29279)
  • abnormal sternum ossification
    • disorganized ossification in the sternum   (MGI Ref ID J:29279)
  • muscle phenotype
  • abnormal intercostal muscle morphology
    • some intercostals muscles associated with ribs 35 are either disorganized or significantly reduced in fiber number at E18.5 and in newborns   (MGI Ref ID J:29279)
  • abnormal myotome development
    • although earliest myotome formation is unaffected, mutants exhibit a deficit in myotome development beginning around E9 and ending around E11   (MGI Ref ID J:29279)
  • abnormal myotome morphology
    • myotomes are greatly reduced in size and cell number at E11   (MGI Ref ID J:29279)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Myf6tm1Wb related

Developmental Biology Research
Mesodermal Defects
      Myogenesis Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Myf6tm1Wb
Allele Name targeted mutation 1, Barbara Wold
Allele Type Targeted (knock-out)
Common Name(s) MRF4bh1;
Mutation Made ByDr. Barbara Wold,   California Institute of Technology
Strain of Origin129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line NameCJ7
ES Cell Line Strain129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name Myf6, myogenic factor 6
Chromosome 10
Gene Common Name(s) CNM3; MRF4; MRF4AA; bHLHc4; herculin; myf-6;
General Note Studies have shown that Myf6 may regulate Myf5 expression by a cis-acting mechanism and that the skeletal abnormalities seen in Myf6 homozygotes may result from a decrease or lack of Myf5 expression (J:29279, J:42453). This allele (Myf6tm1Wb) exhibits normal Myf5 expression at E8 but by E10, expression is reduced (J:29279).
Molecular Note Replacement of DNA sequences encoding codons 40-173 with a neomycin cassette. This mutation eliminates the helix-loop-helix domain of the protein. Expression of Myf5 is seen at E8, however by E10, it is lower and remains lower. [MGI Ref ID J:29279]

Genotyping

Genotyping Information

Genotyping Protocols

Myf6tm1Wb, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Patapoutian A; Yoon JK; Miner JH; Wang S; Stark K; Wold B. 1995. Disruption of the mouse MRF4 gene identifies multiple waves of myogenesis in the myotome. Development 121(10):3347-58. [PubMed: 7588068]  [MGI Ref ID J:29279]

Additional References

Olson EN; Arnold HH; Rigby PW; Wold BJ. 1996. Know your neighbors: three phenotypes in null mutants of the myogenic bHLH gene MRF4. Cell 85(1):1-4. [PubMed: 8620528]  [MGI Ref ID J:32441]

Myf6tm1Wb related

Olson EN; Arnold HH; Rigby PW; Wold BJ. 1996. Know your neighbors: three phenotypes in null mutants of the myogenic bHLH gene MRF4. Cell 85(1):1-4. [PubMed: 8620528]  [MGI Ref ID J:32441]

Yoon JK; Olson EN; Arnold HH; Wold BJ. 1997. Different MRF4 knockout alleles differentially disrupt Myf-5 expression: cis-regulatory interactions at the MRF4/Myf-5 locus. Dev Biol 188(2):349-62. [PubMed: 9268580]  [MGI Ref ID J:42453]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThe strain is maintained by mating heterozygotes and normal wild type siblings. 5/27/97 keb: We are outcrossing to B6 each generation since homozygous mice are lethal at birth.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3175.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4127.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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