Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain C57BL/6 Donor Strain 129S1 via CJ7 ES cell line Generation N10 Generation Definitions   Donating Investigator Barbara Wold,   California Institute of Technology

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Myf6^tm1Wb/Myf6^tm1Wb

        involves: 129S1/Sv * C57BL/6

• lethality-prenatal/perinatal
• neonatal lethality (MGI Ref ID J:29279)
  • die shortly after birth
• respiratory system phenotype
• respiratory distress (MGI Ref ID J:29279)
  • unable to breathe
• skeleton phenotype
• abnormal cervical vertebrae morphology (MGI Ref ID J:29279)
  • the tuberculum anterior is absent on the 6th cervical vertebrae of most mice
• abnormal rib morphology (MGI Ref ID J:29279)
  • rib development is severely disturbed in newborns
  • some ribs display abnormal angles of extension
• abnormal rib-sternum attachment (MGI Ref ID J:29279)
  • rib foreshortening leads to variable failure to join the sternum
• rib bifurcation (MGI Ref ID J:29279)
• rib fusion (MGI Ref ID J:29279)
  • fusion of rib cartilage from adjacent ribs
• short ribs (MGI Ref ID J:29279)
  • rib foreshortening in ribs 2-12 is usually less than 20% of total length and leads to variable failure to join the sternum
  • the first and last ribs are more dramatically shortened compared to others
• truncated ribs (MGI Ref ID J:29279)
  • truncated ribs that fail to attach to the sternum
• abnormal sternum ossification (MGI Ref ID J:29279)
  • disorganized ossification in the sternum
• muscle phenotype
• abnormal intercostal muscle morphology (MGI Ref ID J:29279)
  • some intercostals muscles associated with ribs 3Ñ5 are either disorganized or significantly reduced in fiber number at E18.5 and in newborns
• abnormal myotome development (MGI Ref ID J:29279)
  • although earliest myotome formation is unaffected, mutants exhibit a deficit in myotome development beginning around E9 and ending around E11
• abnormal myotome morphology (MGI Ref ID J:29279)
  • myotomes are greatly reduced in size and cell number at E11

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Myf6^tm1Wb related

Developmental Biology Research
Mesodermal Defects
      Myogenesis Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Myf6tm1Wb
Allele Name		targeted mutation 1, Barbara Wold
Allele Type		Targeted (knock-out)
Common Name(s)		MRF4bh1;
Mutation Made By		Barbara Wold,   California Institute of Technology
Strain of Origin		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line Name		CJ7
ES Cell Line Strain		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name		Myf6, myogenic factor 6
Chromosome		10
Gene Common Name(s)		MRF4; MRF4AA; bHLHc4; herculin;
General Note		Studies have shown that Myf6 may regulate Myf5 expression by a cis-acting mechanism and that the skeletal abnormalities seen in Myf6 homozygotes may result from a decrease or lack of Myf5 expression (J:29279, J:42453). This allele (Myf6tm1Wb) exhibits normal Myf5 expression at E8 but by E10, expression is reduced (J:29279).
Molecular Note		Replacement of DNA sequences encoding codons 40-173 with a neomycin cassette. This mutation eliminates the helix-loop-helix domain of the protein. Expression of Myf5 is seen at E8, however by E10, it is lower and remains lower. [MGI Ref ID J:29279]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Patapoutian A; Yoon JK; Miner JH; Wang S; Stark K; Wold B. 1995. Disruption of the mouse MRF4 gene identifies multiple waves of myogenesis in the myotome. Development 121(10):3347-58. [PubMed: 7588068]  [MGI Ref ID J:29279]

Additional References

Olson EN; Arnold HH; Rigby PW; Wold BJ. 1996. Know your neighbors: three phenotypes in null mutants of the myogenic bHLH gene MRF4. Cell 85(1):1-4. [PubMed: 8620528]  [MGI Ref ID J:32441]

Myf6^tm1Wb related

Olson EN; Arnold HH; Rigby PW; Wold BJ. 1996. Know your neighbors: three phenotypes in null mutants of the myogenic bHLH gene MRF4. Cell 85(1):1-4. [PubMed: 8620528]  [MGI Ref ID J:32441]

Yoon JK; Olson EN; Arnold HH; Wold BJ. 1997. Different MRF4 knockout alleles differentially disrupt Myf-5 expression: cis-regulatory interactions at the MRF4/Myf-5 locus. Dev Biol 188(2):349-62. [PubMed: 9268580]  [MGI Ref ID J:42453]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.