Description

Strain Information

Former Names B6;129S7-Ptgs2tm1Jed/J    (Changed: 12-JUN-08 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse   Donating Investigator Joe Dinchuk,   The Dupont Merck Pharmaceutical Company

Description
Mice homozygous for the Ptgs2^tm1Jed targeted mutation exhibit significant preweaning loss of homozygotes (original publication reports 30-40%). Homozygous mutant mice show polydipsia and polyuria due to a defect in renal development. Cardiac fibrosis is evident in approximately 50% of the mice. PTGS2 deficient mice do not show altered inflammatory responses to in several tests of paw and ear edema; however, cytoxicity of hepatic cells induced by endotoxin was strikingly mitigated in these homozygotes. Female homozygotes are infertile with defects in ovulation, fertilization, implantation, and decidualization.

Development
This strain was developed in the lab of Dr. Joe Dinchuk at Glenolden Laboratory, Dupont Merck Pharmaceutical Company. The transcription/translation start sites for the endogenous Ptgs2 gene have been removed. The 129S7-derived AB2.1 ES cell line was used. At The Jackson Laboratory, these mice were bred with B6129SF1/J (Stock No. 101043) to maintain the colony.

Control Information

	Control
	Wild-type from the colony
	101045 B6129SF2/J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Ptgs2

008104	B6.129(FVB)-Ptgs2tm2.1(Ptgs1)Fun/J
008101	B6.129S6(FVB)-Ptgs2tm1.1Fun/J

View Strains carrying other alleles of Ptgs2     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Ptgs2^tm1Jed/Ptgs2^tm1Jed

        involves: 129S7/SvEvBrd * C57BL/6

• reproductive system phenotype
• *normal* reproductive system phenotype (MGI Ref ID J:69034)
  • in vitro, oocyte-cumulus cell complexes retrieved from eCG-primed ovaries of adult homozygotes display normal follicular growth and oocyte maturation
  • also, in vitro-matured oocytes obtained from adult female homozygotes show no significant differences in fertilization or preimplantation development
• abnormal decidualization (MGI Ref ID J:43609)
  • unlike wild-type mice, pseudopregnant female homozygotes fail to exhibit an increase in uterine weight in response to intraluminal infusion of oil (a deciduogenic stimulus)
  • intraluminal infusion of PGI2 (but not PGE2) or cholera toxin as the deciduogenic stimulus partially restores decidualization in ovaroectomized steroid hormonally prepared mutant mice
• decreased litter size (MGI Ref ID J:118171)
  • homozygous females produce small litters compared to wild-type
• decreased ovulation frequency (MGI Ref ID J:69034)
  • adult (2- to 8-month-old) female homozygotes exhibit a poor ovulation rate upon induction with eCG and hCG
  • immature (3-week-old) female homozygotes exhibit a superior ovulation rate relative to adult homozygotes, suggesting that the ovulatory process becomes defective with aging
  • females homozygotes exhibit significantly reduced ovulation, despite normal ovarian response to gonadotropins
  • in addition, any recovered eggs appear developmentally abnormal, displaying virtually no extrusion of the first polar body
• impaired fertilization (MGI Ref ID J:43609)
  • female homozygotes display complete failure of fertilization, despite the presence of numerous sperm at the fertilization site
• reduced female fertility (MGI Ref ID J:109548)
  • number of term pregnancies reduced
  • only 20% of homozygous females can sustain term pregnancy
• embryogenesis phenotype
• failure of implantation (MGI Ref ID J:69034)
  • in blastocyst transfer experiments, wild-type blastocysts fail to exhibit the initial attachment reaction and do not implant into uteri of pseudopregnant female homozygous mutant mice
  • failure of implantation and subsequent decidualization occur despite normal uterine responsiveness to steroid hormones
• renal/urinary system phenotype
• abnormal kidney cortex (MGI Ref ID J:109548)
  • atrophic
• abnormal renal glomerulus morphology (MGI Ref ID J:109548)
  • hypoplastic
  • average glomerular diameter is reduced to 29.4 um from 39.2 um in wild-type
• cortical renal glomerulopathies (MGI Ref ID J:118171)
  • in mice older than 6 weeks, renal cortex is abnormal with small immature glomeruli and deteriorating tubules; these changes are not seen in 2-month old COX-1 knockin mice
• glomerulosclerosis (MGI Ref ID J:109548)
  • moderate
• pale kidney (MGI Ref ID J:109548)
• small kidney (MGI Ref ID J:109548)
• cardiovascular system phenotype
• abnormal PGE2 physiology (MGI Ref ID J:109548)
  • near absence of LPS induced synthesis
• decreased vascular permeability (MGI Ref ID J:118171)
  • dye extravasation in the ear vasculature is decreased by 50-60% with bradykinin challenge compared to wild-type
• patent ductus arteriosus (MGI Ref ID J:109548)
  • fails to close in 40% of mice
• digestive/alimentary phenotype
• peritoneal inflammation (MGI Ref ID J:118171)
  • at 5 months of age, mice show chronic peritonitis
• homeostasis/metabolism phenotype
• increased blood urea nitrogen level (MGI Ref ID J:118171)
  • BUN levels are increased ~2-fold vs wild-type at 6 months of age
• immune system phenotype
• peritoneal inflammation (MGI Ref ID J:118171)
  • at 5 months of age, mice show chronic peritonitis

Ptgs2^tm1Jed/Ptgs2^tm1Jed

        B6;129S-Ptgs2^tm1Jed/J

• respiratory system phenotype
• pulmonary interstitial fibrosis (MGI Ref ID J:68669)
  • in response to bleomycin-induced lung injury, homozygotes exhibit an aggressive fibroproliferative response, widespread inflammation, loss of alveolar architecture, and increased extracellular matrix protein deposition relative to wild-type mice

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Ptgs2^tm1Jed/Ptgs2^tm1Jed

        involves: 129S7/SvEvBrd

• lethality-prenatal/perinatal
• neonatal lethality (MGI Ref ID J:29974)
  • ~65% of homozygous mutant mice die neonatally
• life span-post-weaning/aging
• premature death (MGI Ref ID J:29974)
  • homozygotes that reach weaning have an average lifespan of ~3.5 months, with a few animals surviving beyond 6 months
• renal/urinary system phenotype
• abnormal kidney morphology (MGI Ref ID J:29974)
  • all adult homozygotes exhibit renal dysplasia
• abnormal kidney collecting duct (MGI Ref ID J:29974)
  • homozygous newborns show poor collecting duct development and abundant undifferentiated mesenchyme
• abnormal kidney cortex (MGI Ref ID J:29974)
• kidney cortex cysts (MGI Ref ID J:29974)
  • all adult homozygotes display corticomedullary microcysts
• abnormal kidney development (MGI Ref ID J:29974)
  • although fetal metanephri appear normal at E14, kidneys of homozygous newborns appear severely underdeveloped relative to wild-type kidneys
• abnormal kidney medulla morphology (MGI Ref ID J:29974)
  • homozygous newborns exhibit corticomedullary atrophy
• kidney medulla hypoplasia (MGI Ref ID J:29974)
  • all adult homozygotes display mild medullary hypoplasia or atrophy
• abnormal renal glomerulus morphology (MGI Ref ID J:29974)
  • all adult homozygotes exhibit mild to moderate renal lesions typified by numerous immature small glomeruli found subcapsularly
• abnormal renal tubule morphology (MGI Ref ID J:29974)
  • all adult homozygotes exhibit mild to moderate renal lesions typified by multiple foci of dysplastic tubules
• kidney failure (MGI Ref ID J:29974)
  • adult homozygotes die of chronic renal failure of developmental origin
• kidney inflammation (MGI Ref ID J:29974)
  • all adult homozygotes with renal histopathology are susceptible to development of secondary pyelonephritis
• reproductive system phenotype
• absent corpus luteum (MGI Ref ID J:29974)
  • mutant ovaries show virtual absence of corpora lutea, despite normal ovarian follicular development
• decreased ovulation frequency (MGI Ref ID J:29974)
• female infertility (MGI Ref ID J:29974)
  • homozygous females are largely infertile, rarely giving birth to live offspring
• small ovary (MGI Ref ID J:29974)
• endocrine/exocrine gland phenotype
• absent corpus luteum (MGI Ref ID J:29974)
  • mutant ovaries show virtual absence of corpora lutea, despite normal ovarian follicular development
• small ovary (MGI Ref ID J:29974)
• cardiovascular system phenotype
• cardiac fibrosis (MGI Ref ID J:29974)
  • 50% of adult homozygotes exhibit diffuse myocardial fibrosis of variable severity involving both right and left ventricles
• homeostasis/metabolism phenotype
• increased blood urea nitrogen level (MGI Ref ID J:29974)
• increased circulating creatinine level (MGI Ref ID J:29974)
• immune system phenotype
• *normal* immune system phenotype (MGI Ref ID J:29974)
  • homozygotes exhibit normal immune responses to carrageenan-induced paw edema, TPA-induced edema and arachidonic acid-induced edema
• kidney inflammation (MGI Ref ID J:29974)
  • all adult homozygotes with renal histopathology are susceptible to development of secondary pyelonephritis
• hematopoietic system phenotype
• *normal* hematopoietic system phenotype (MGI Ref ID J:29974)
  • homozygotes exhibit normal hematologic parameters

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Ptgs2^tm1Jed related

Developmental Biology Research
Internal/Organ Defects (kidney: vasculature)

Immunology and Inflammation Research
Inflammation

Internal/Organ Research
Kidney Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Ptgs2tm1Jed
Allele Name		targeted mutation 1, Joe E Dinchuk
Allele Type		Targeted (knock-out)
Common Name(s)		COX-2-; COX2-; Ptgs2-;
Mutation Made By		Joe Dinchuk,   The Dupont Merck Pharmaceutical Company
Strain of Origin		129S7/SvEvBrd-Hprt1
ES Cell Line Name		AB2.1
ES Cell Line Strain		129S7/SvEvBrd-Hprt1
Gene Symbol and Name		Ptgs2, prostaglandin-endoperoxide synthase 2
Chromosome		1
Gene Common Name(s)		COX-2; COX2; GRIPGHS; PGG/HS; PGHS-2; PHS-2; Pghs2; Tis10; cyclooxygenase 2; cyclooxygenase-2; hCox-2; prostaglandin G/H synthase;
Molecular Note		A 1.8 kb genomic fragment containing exon 1 and the transcription and translation start sites was replaced with a neomycin selection cassette. Northern blot analysis on RNA derived from embryonic fibroblasts derived from homozygous embryos demonstrated that no detectable transcript was produced from this allele. [MGI Ref ID J:29974]

Genotyping

Genotyping Information

Genotyping Protocols

Ptgs2^tm1Jed, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Dinchuk JE; Car BD; Focht RJ; Johnston JJ; Jaffee BD; Covington MB; Contel NR; Eng VM; Collins RJ; Czerniak PM; Gorry SA; Trzaskos JM.. 1995. Renal abnormalities and an altered inflammatory response in mice lacking cyclooxygenase II. Nature 378(6555):406-9. [PubMed: 7477380]  [MGI Ref ID J:29974]

Additional References

Ethridge RT; Chung DH; Slogoff M; Ehlers RA; Hellmich MR; Rajaraman S; Saito H; Uchida T; Evers BM. 2002. Cyclooxygenase-2 gene disruption attenuates the severity of acute pancreatitis and pancreatitis-associated lung injury. Gastroenterology 123(4):1311-22. [PubMed: 12360491]  [MGI Ref ID J:79333]

Hunot S; Vila M; Teismann P; Davis RJ; Hirsch EC; Przedborski S; Rakic P; Flavell RA. 2004. JNK-mediated induction of cyclooxygenase 2 is required for neurodegeneration in a mouse model of Parkinson's disease. Proc Natl Acad Sci U S A 101(2):665-70. [PubMed: 14704277]  [MGI Ref ID J:87428]

Keerthisingam CB; Jenkins RG; Harrison NK; Hernandez-Rodriguez NA; Booth H; Laurent GJ; Hart SL; Foster ML; McAnulty RJ. 2001. Cyclooxygenase-2 deficiency results in a loss of the anti-proliferative response to transforming growth factor-beta in human fibrotic lung fibroblasts and promotes bleomycin-induced pulmonary fibrosis in mice. Am J Pathol 158(4):1411-22. [PubMed: 11290559]  [MGI Ref ID J:68669]

Oshima M; Dinchuk JE; Kargman SL; Oshima H; Hancock B; Kwong E; Trzaskos JM; Evans JF; Taketo MM. 1996. Suppression of intestinal polyposis in Apc delta716 knockout mice by inhibition of cyclooxygenase 2 (COX-2). Cell 87(5):803-9. [PubMed: 8945508]  [MGI Ref ID J:36816]

Parman T; Wells PG. 2002. Embryonic prostaglandin H synthase-2 (PHS-2) expression and benzo[a]pyrene teratogenicity in PHS-2 knockout mice. FASEB J 16(9):1001-9. [PubMed: 12087061]  [MGI Ref ID J:77471]

Russell DL; Doyle KM; Ochsner SA; Sandy JD; Richards JS. 2003. Processing and localization of ADAMTS-1 and proteolytic cleavage of versican during cumulus matrix expansion and ovulation. J Biol Chem 278(43):42330-9. [PubMed: 12907688]  [MGI Ref ID J:86134]

Ptgs2^tm1Jed related

Alam I; Warden SJ; Robling AG; Turner CH. 2005. Mechanotransduction in bone does not require a functional cyclooxygenase-2 (COX-2) gene. J Bone Miner Res 20(3):438-46. [PubMed: 15746988]  [MGI Ref ID J:111300]

Anning PB; Coles B; Morton J; Wang H; Uddin J; Morrow JD; Dey SK; Marnett LJ; O'Donnell VB. 2006. Nitric oxide deficiency promotes vascular side effects of cyclooxygenase inhibitors. Blood 108(13):4059-62. [PubMed: 16931629]  [MGI Ref ID J:140540]

Baumgartner HK; Starodub OT; Joehl JS; Tackett L; Montrose MH. 2004. Cyclooxygenase 1 is required for pH control at the mouse gastric surface. Gut 53(12):1751-7. [PubMed: 15542509]  [MGI Ref ID J:103642]

Burleigh ME; Babaev VR; Oates JA; Harris RC; Gautam S; Riendeau D; Marnett LJ; Morrow JD; Fazio S; Linton MF. 2002. Cyclooxygenase-2 promotes early atherosclerotic lesion formation in LDL receptor-deficient mice. Circulation 105(15):1816-23. [PubMed: 11956125]  [MGI Ref ID J:103220]

Burleigh ME; Babaev VR; Yancey PG; Major AS; McCaleb JL; Oates JA; Morrow JD; Fazio S; Linton MF. 2005. Cyclooxygenase-2 promotes early atherosclerotic lesion formation in ApoE-deficient and C57BL/6 mice. J Mol Cell Cardiol 39(3):443-52. [PubMed: 16040051]  [MGI Ref ID J:103979]

Cheng HF; Wang JL; Zhang MZ; Wang SW; McKanna JA; Harris RC. 2001. Genetic deletion of COX-2 prevents increased renin expression in response to ACE inhibition. Am J Physiol Renal Physiol 280(3):F449-56. [PubMed: 11181406]  [MGI Ref ID J:114283]

Cheng JG; Stewart CL. 2003. Loss of cyclooxygenase-2 retards decidual growth but does not inhibit embryo implantation or development to term. Biol Reprod 68(2):401-4. [PubMed: 12533402]  [MGI Ref ID J:81389]

Ejima K; Layne MD; Carvajal IM; Kritek PA; Baron RM; Chen YH; Vom Saal J; Levy BD; Yet SF; Perrella MA. 2003. Cyclooxygenase-2-deficient mice are resistant to endotoxin-induced inflammation and death. FASEB J 17(10):1325-7. [PubMed: 12738799]  [MGI Ref ID J:118510]

Ethridge RT; Chung DH; Slogoff M; Ehlers RA; Hellmich MR; Rajaraman S; Saito H; Uchida T; Evers BM. 2002. Cyclooxygenase-2 gene disruption attenuates the severity of acute pancreatitis and pancreatitis-associated lung injury. Gastroenterology 123(4):1311-22. [PubMed: 12360491]  [MGI Ref ID J:79333]

Fong LY; Jiang Y; Riley M; Liu X; Smalley KJ; Guttridge DC; Farber JL. 2008. Prevention of upper aerodigestive tract cancer in zinc-deficient rodents: inefficacy of genetic or pharmacological disruption of COX-2. Int J Cancer 122(5):978-89. [PubMed: 17985342]  [MGI Ref ID J:135556]

Fukunaga K; Kohli P; Bonnans C; Fredenburgh LE; Levy BD. 2005. Cyclooxygenase 2 plays a pivotal role in the resolution of acute lung injury. J Immunol 174(8):5033-9. [PubMed: 15814734]  [MGI Ref ID J:98151]

Hamada T; Tsuchihashi S; Avanesyan A; Duarte S; Moore C; Busuttil RW; Coito AJ. 2008. Cyclooxygenase-2 deficiency enhances Th2 immune responses and impairs neutrophil recruitment in hepatic ischemia/reperfusion injury. J Immunol 180(3):1843-53. [PubMed: 18209082]  [MGI Ref ID J:131501]

Hodges RJ; Jenkins RG; Wheeler-Jones CP; Copeman DM; Bottoms SE; Bellingan GJ; Nanthakumar CB; Laurent GJ; Hart SL; Foster ML; McAnulty RJ. 2004. Severity of lung injury in cyclooxygenase-2-deficient mice is dependent on reduced prostaglandin E(2) production. Am J Pathol 165(5):1663-76. [PubMed: 15509536]  [MGI Ref ID J:93638]

Howe LR; Chang SH; Tolle KC; Dillon R; Young LJ; Cardiff RD; Newman RA; Yang P; Thaler HT; Muller WJ; Hudis C; Brown AM; Hla T; Subbaramaiah K; Dannenberg AJ. 2005. HER2/neu-induced mammary tumorigenesis and angiogenesis are reduced in cyclooxygenase-2 knockout mice. Cancer Res 65(21):10113-9. [PubMed: 16267038]  [MGI Ref ID J:102694]

Hunot S; Vila M; Teismann P; Davis RJ; Hirsch EC; Przedborski S; Rakic P; Flavell RA. 2004. JNK-mediated induction of cyclooxygenase 2 is required for neurodegeneration in a mouse model of Parkinson's disease. Proc Natl Acad Sci U S A 101(2):665-70. [PubMed: 14704277]  [MGI Ref ID J:87428]

Im JY; Kim D; Paik SG; Han PL. 2006. Cyclooxygenase-2-dependent neuronal death proceeds via superoxide anion generation. Free Radic Biol Med 41(6):960-72. [PubMed: 16934679]  [MGI Ref ID J:112581]

Keerthisingam CB; Jenkins RG; Harrison NK; Hernandez-Rodriguez NA; Booth H; Laurent GJ; Hart SL; Foster ML; McAnulty RJ. 2001. Cyclooxygenase-2 deficiency results in a loss of the anti-proliferative response to transforming growth factor-beta in human fibrotic lung fibroblasts and promotes bleomycin-induced pulmonary fibrosis in mice. Am J Pathol 158(4):1411-22. [PubMed: 11290559]  [MGI Ref ID J:68669]

Kellogg AP; Wiggin TD; Larkin DD; Hayes JM; Stevens MJ; Pop-Busui R. 2007. Protective effects of cyclooxygenase-2 gene inactivation against peripheral nerve dysfunction and intraepidermal nerve fiber loss in experimental diabetes. Diabetes 56(12):2997-3005. [PubMed: 17720896]  [MGI Ref ID J:132324]

Kim SM; Chen L; Mizel D; Huang YG; Briggs JP; Schnermann J. 2007. Low plasma renin and reduced renin secretory responses to acute stimuli in conscious COX-2-deficient mice. Am J Physiol Renal Physiol 292(1):F415-22. [PubMed: 16954340]  [MGI Ref ID J:118087]

Lim H; Gupta RA; Ma WG; Paria BC; Moller DE; Morrow JD; DuBois RN; Trzaskos JM; Dey SK. 1999. Cyclo-oxygenase-2-derived prostacyclin mediates embryo implantation in the mouse via PPARdelta. Genes Dev 13(12):1561-74. [PubMed: 10385625]  [MGI Ref ID J:56144]

Lim H; Paria BC; Das SK; Dinchuk JE; Langenbach R; Trzaskos JM; Dey SK. 1997. Multiple female reproductive failures in cyclooxygenase 2-deficient mice. Cell 91(2):197-208. [PubMed: 9346237]  [MGI Ref ID J:43609]

Liu H; Ye W; Guan G; Dong Z; Jia Z; Yang T. 2007. Developmental regulation of calcineurin isoforms in the rodent kidney: association with COX-2. Am J Physiol Renal Physiol 293(6):F1898-904. [PubMed: 17881460]  [MGI Ref ID J:127529]

Lorenz M; Slaughter HS; Wescott DM; Carter SI; Schnyder B; Dinchuk JE; Car BD. 1999. Cyclooxygenase-2 is essential for normal recovery from 5-fluorouracil-induced myelotoxicity in mice. Exp Hematol 27(10):1494-502. [PubMed: 10517490]  [MGI Ref ID J:115084]

Matsumoto H; Ma W; Smalley W; Trzaskos J; Breyer RM; Dey SK. 2001. Diversification of cyclooxygenase-2-derived prostaglandins in ovulation and implantation. Biol Reprod 64(5):1557-65. [PubMed: 11319164]  [MGI Ref ID J:69034]

Matsumoto H; Ma WG; Daikoku T; Zhao X; Paria BC; Das SK; Trzaskos JM; Dey SK. 2002. Cyclooxygenase-2 differentially directs uterine angiogenesis during implantation in mice. J Biol Chem 277(32):29260-7. [PubMed: 12034746]  [MGI Ref ID J:78279]

Moeckel GW; Zhang L; Fogo AB; Hao CM; Pozzi A; Breyer MD. 2003. COX2 activity promotes organic osmolyte accumulation and adaptation of renal medullary interstitial cells to hypertonic stress. J Biol Chem 278(21):19352-7. [PubMed: 12637551]  [MGI Ref ID J:83578]

Muller-Decker K; Furstenberger G. 2007. The cyclooxygenase-2-mediated prostaglandin signaling is causally related to epithelial carcinogenesis. Mol Carcinog 46(8):705-10. [PubMed: 17546626]  [MGI Ref ID J:126154]

Norwood VF; Morham SG; Smithies O. 2000. Postnatal development and progression of renal dysplasia in cyclooxygenase-2 null mice. Kidney Int 58(6):2291-300. [PubMed: 11115063]  [MGI Ref ID J:104002]

Ochsner SA; Russell DL; Day AJ; Breyer RM; Richards JS. 2003. Decreased expression of tumor necrosis factor-alpha-stimulated gene 6 in cumulus cells of the cyclooxygenase-2 and EP2 null mice. Endocrinology 144(3):1008-19. [PubMed: 12586778]  [MGI Ref ID J:115517]

Oshima M; Dinchuk JE; Kargman SL; Oshima H; Hancock B; Kwong E; Trzaskos JM; Evans JF; Taketo MM. 1996. Suppression of intestinal polyposis in Apc delta716 knockout mice by inhibition of cyclooxygenase 2 (COX-2). Cell 87(5):803-9. [PubMed: 8945508]  [MGI Ref ID J:36816]

Paliege A; Mizel D; Medina C; Pasumarthy A; Huang YG; Bachmann S; Briggs JP; Schnermann JB; Yang T. 2004. Inhibition of nNOS expression in the macula densa by COX-2-derived prostaglandin E(2). Am J Physiol Renal Physiol 287(1):F152-9. [PubMed: 15010356]  [MGI Ref ID J:113758]

Parman T; Wells PG. 2002. Embryonic prostaglandin H synthase-2 (PHS-2) expression and benzo[a]pyrene teratogenicity in PHS-2 knockout mice. FASEB J 16(9):1001-9. [PubMed: 12087061]  [MGI Ref ID J:77471]

Patel NS; Cuzzocrea S; Collino M; Chaterjee PK; Mazzon E; Britti D; Yaqoob MM; Thiemermann C. 2007. The role of cycloxygenase-2 in the rodent kidney following ischaemia/reperfusion injury in vivo. Eur J Pharmacol 562(1-2):148-54. [PubMed: 17343844]  [MGI Ref ID J:124476]

Poon R; Smits R; Li C; Jagmohan-Changur S; Kong M; Cheon S; Yu C; Fodde R; Alman BA. 2001. Cyclooxygenase-two (COX-2) modulates proliferation in aggressive fibromatosis (desmoid tumor). Oncogene 20(4):451-60. [PubMed: 11313976]  [MGI Ref ID J:69222]

Sadikot RT; Zeng H; Azim AC; Joo M; Dey SK; Breyer RM; Peebles RS; Blackwell TS; Christman JW. 2007. Bacterial clearance of Pseudomonas aeruginosa is enhanced by the inhibition of COX-2. Eur J Immunol 37(4):1001-9. [PubMed: 17330822]  [MGI Ref ID J:120725]

Schwarz NT; Kalff JC; Turler A; Engel BM; Watkins SC; Billiar TR; Bauer AJ. 2001. Prostanoid production via COX-2 as a causative mechanism of rodent postoperative ileus. Gastroenterology 121(6):1354-71. [PubMed: 11729115]  [MGI Ref ID J:107752]

Seno H; Oshima M; Ishikawa TO; Oshima H; Takaku K; Chiba T; Narumiya S; Taketo MM. 2002. Cyclooxygenase 2- and prostaglandin E(2) receptor EP(2)-dependent angiogenesis in Apc(Delta716) mouse intestinal polyps. Cancer Res 62(2):506-11. [PubMed: 11809702]  [MGI Ref ID J:74003]

Shimada M; Hernandez-Gonzalez I; Gonzalez-Robayna I; Richards JS. 2006. Paracrine and autocrine regulation of epidermal growth factor-like factors in cumulus oocyte complexes and granulosa cells: key roles for prostaglandin synthase 2 and progesterone receptor. Mol Endocrinol 20(6):1352-65. [PubMed: 16543407]  [MGI Ref ID J:108939]

Stevens HY; Meays DR; Yeh J; Bjursten LM; Frangos JA. 2006. COX-2 is necessary for venous ligation-mediated bone adaptation in mice. Bone 38(1):93-104. [PubMed: 16122997]  [MGI Ref ID J:104470]

Subbaramaiah K; Howe LR; Port ER; Brogi E; Fishman J; Liu CH; Hla T; Hudis C; Dannenberg AJ. 2006. HER-2/neu status is a determinant of mammary aromatase activity in vivo: evidence for a cyclooxygenase-2-dependent mechanism. Cancer Res 66(10):5504-11. [PubMed: 16707480]  [MGI Ref ID J:109047]

Takabayshi K; Corr M; Hayashi T; Redecke V; Beck L; Guiney D; Sheppard D; Raz E. 2006. Induction of a homeostatic circuit in lung tissue by microbial compounds. Immunity 24(4):475-87. [PubMed: 16618605]  [MGI Ref ID J:113350]

Takeda H; Sonoshita M; Oshima H; Sugihara K; Chulada PC; Langenbach R; Oshima M; Taketo MM. 2003. Cooperation of cyclooxygenase 1 and cyclooxygenase 2 in intestinal polyposis. Cancer Res 63(16):4872-7. [PubMed: 12941808]  [MGI Ref ID J:85136]

Taketo MM. 2006. Mouse models of gastrointestinal tumors. Cancer Sci 97(5):355-61. [PubMed: 16630131]  [MGI Ref ID J:116407]

Tuo J; Tuaillon N; Shen D; Chan CC. 2004. Endotoxin-induced uveitis in cyclooxygenase-2-deficient mice. Invest Ophthalmol Vis Sci 45(7):2306-13. [PubMed: 15223810]  [MGI Ref ID J:109715]

Udd L; Katajisto P; Rossi DJ; Lepisto A; Lahesmaa AM; Ylikorkala A; Jarvinen HJ; Ristimaki AP; Makela TP. 2004. Suppression of Peutz-Jeghers polyposis by inhibition of cyclooxygenase-2. Gastroenterology 127(4):1030-7. [PubMed: 15480979]  [MGI Ref ID J:93361]

Williams CS; Tsujii M; Reese J; Dey SK; DuBois RN. 2000. Host cyclooxygenase-2 modulates carcinoma growth J Clin Invest 105(11):1589-94. [PubMed: 10841517]  [MGI Ref ID J:62763]

Yang H; Zhang J; Andreasson K; Chen C. 2008. COX-2 oxidative metabolism of endocannabinoids augments hippocampal synaptic plasticity. Mol Cell Neurosci 37(4):682-95. [PubMed: 18295507]  [MGI Ref ID J:135668]

Yang T; Endo Y; Huang YG; Smart A; Briggs JP; Schnermann J. 2000. Renin expression in COX-2-knockout mice on normal or low-salt diets. Am J Physiol Renal Physiol 279(5):F819-25. [PubMed: 11053041]  [MGI Ref ID J:114184]

Yang T; Huang YG; Ye W; Hansen P; Schnermann JB; Briggs JP. 2005. Influence of genetic background and gender on hypertension and renal failure in COX-2-deficient mice. Am J Physiol Renal Physiol 288(6):F1125-32. [PubMed: 15613621]  [MGI Ref ID J:98504]

Yang WL; Cai KQ; Smedberg JL; Smith ER; Klein-Szanto A; Hamilton TC; Xu XX. 2007. A reduction of cyclooxygenase 2 gene dosage counters the ovarian morphological aging and tumor phenotype in Wv mice. Am J Pathol 170(4):1325-36. [PubMed: 17392171]  [MGI Ref ID J:120134]

Yao B; Xu J; Harris RC; Zhang MZ. 2008. Renal localization and regulation of 15-hydroxyprostaglandin dehydrogenase. Am J Physiol Renal Physiol 294(2):F433-9. [PubMed: 18057186]  [MGI Ref ID J:130424]

Yokota T; Meka CS; Medina KL; Igarashi H; Comp PC; Takahashi M; Nishida M; Oritani K; Miyagawa J; Funahashi T; Tomiyama Y; Matsuzawa Y; Kincade PW. 2002. Paracrine regulation of fat cell formation in bone marrow cultures via adiponectin and prostaglandins. J Clin Invest 109(10):1303-10. [PubMed: 12021245]  [MGI Ref ID J:140382]

Yu J; Wu CW; Chu ES; Hui AY; Cheng AS; Go MY; Ching AK; Chui YL; Chan HL; Sung JJ. 2008. Elucidation of the role of COX-2 in liver fibrogenesis using transgenic mice. Biochem Biophys Res Commun 372(4):571-7. [PubMed: 18503750]  [MGI Ref ID J:137793]

Yu Y; Fan J; Chen XS; Wang D; Klein-Szanto AJ; Campbell RL; Fitzgerald GA; Funk CD. 2006. Genetic model of selective COX2 inhibition reveals novel heterodimer signaling. Nat Med 12(6):699-704. [PubMed: 16732282]  [MGI Ref ID J:109548]

Yu Y; Fan J; Hui Y; Rouzer CA; Marnett LJ; Klein-Szanto AJ; FitzGerald GA; Funk CD. 2007. Targeted cyclooxygenase gene (ptgs) exchange reveals discriminant isoform functionality. J Biol Chem 282(2):1498-506. [PubMed: 17110378]  [MGI Ref ID J:118171]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Wild-type from the colony
	101045 B6129SF2/J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.