Description

Strain Information

Type Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation F183

Appearance
agouti, cataract
Related Genotype: A/A Cryaa^lop18/Cryaa^lop18

Description
Mice homozygous for Cryaa^lop18 develop large, dense nuclear cataracts. At embryonic day (E) 10 the lens vesicle in these homozygotes buds out from the surface ectoderm. At E14 tiny vacuoles develop in the lens, these are larger at E16, and by four months of age there is advanced degeneration of the cortex with posterior migration of the lens epithelial nuclei and the posterior pole of the lens shows abnormal lens fibers. However, the cataracts were not found to progress further after this age. (Chang et al., 1996.)

Development
The recessive Cryaa^lop18 mutation arose spontaneously and was identified in the inbred strain CBA/CaGnLe at The Jackson Laboratory in approximately 1993. In 1995 embryos from homozygous parents were cryopreserved. The CBA/CaGnLeJ strain, Stock No. 001143, does not carry this mutation.

Control Information

	Control
	001143 CBA/CaGnLeJ
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Cryaa

005564	FVB(Cg)-Tg(Ins2-CALM1)26Ove Tg(Cryaa-TAg)1Ove/PneJ
002456	FVB/N-Tg(CryHIVPR)72Gjj/J

View Strains carrying other alleles of Cryaa     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms

Crystallin, Alpha-A; CRYAA - Models with phenotypic similarity to human disease where etiologies involve orthologs.1

1 Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Cryaa^lop18/Cryaa^lop18

        CBA/CaGnLeJ

• vision/eye phenotype
• abnormal lens morphology (MGI Ref ID J:34933)
  • vacuoles in lens noted at E14; prominent by E16
  • degeneration of lens cortex
  • posterior migration of lens epithelial nuclei
• abnormal lens fiber morphology (MGI Ref ID J:34933)
  • abnormal fibers form at posterior pole of lens
• cataracts (MGI Ref ID J:34933)
  • apparent at weaning age; progressive severity with age

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cryaa^lop18 related

Sensorineural Research
Cataracts (nuclear with late cortical)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Cryaalop18
Allele Name		lens opacity 18
Allele Type		Spontaneous
Common Name(s)		alphaA-His;
Strain of Origin		CBA/CaGnLeJ
Gene Symbol and Name		Cryaa, crystallin, alpha A
Chromosome		17
Gene Common Name(s)		AI323437; Acry-1; CRYA1; Crya-1; Crya1; DAcry-1; HSPB4; alpha-A-crystallin; crystallin, alpha 1; expressed sequence AI323437; lens opacity 18; lop18;
Molecular Note		A G-to-A transition at nucleotide 161 of the mRNA that converts codon 54 from an arginine to a histidine. [MGI Ref ID J:59482]

Genotyping

Genotyping Information

Genotyping Protocols

Cryaa^lop18, PYRO, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Chang B; Hawes NL; Smith RS; Heckenlively JR; Davisson MT; Roderick TH. 1996. Chromosomal localization of a new mouse lens opacity gene (lop18). (Correction: vol. 39(2):237) Genomics 36(1):171-3. [PubMed: 8812430]  [MGI Ref ID J:34933]

Additional References

Chang B; Hawes NL; Roderick TH; Smith RS; Heckenlively JR; Horwitz J; Davisson MT. 1999. Identification of a missense mutation in the alphaA-crystallin gene of the lop18 mouse. Mol Vis 5:21. [PubMed: 10493778]  [MGI Ref ID J:59482]

Cryaa^lop18 related

Chang B; Hawes NL; Roderick TH; Smith RS; Heckenlively JR; Horwitz J; Davisson MT. 1999. Identification of a missense mutation in the alphaA-crystallin gene of the lop18 mouse. Mol Vis 5:21. [PubMed: 10493778]  [MGI Ref ID J:59482]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Eye Mutant Resource within the Mouse Mutant Resource collection.

Control Information

	Control
	001143 CBA/CaGnLeJ
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

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