Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N2F1N1p

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Mbp

005226	BALB/cJ-Mbpshi-J/J
001428	C3Fe.SWV-Mbpshi/J
004223	CHa.SWV(C3Fe)-Mbpshi/J

View Strains carrying other alleles of Mbp     (3 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Mbp^shi-mld related

Neurobiology Research
Myelination Defects
Tremor Defects
Vestibular and Hearing Defects

Sensorineural Research
Vestibular and Hearing Defects

Currently there is no phenotype information for this strain.

Genes & Alleles

Gene & Allele Information

Allele Symbol		Mbpshi-mld
Allele Name		myelin deficient
Allele Type		Spontaneous
Strain of Origin		MDB/Dt
Gene Symbol and Name		Mbp, myelin basic protein
Chromosome		18
Gene Common Name(s)		C76307; Hmbpr; MGC99675; R75289; expressed sequence C76307; expressed sequence R75289; golli-mbp; hemopoietic myelin basic protein related; mld; myelin deficient; shi; shiverer;
General Note		Mbpshi-mld, myelin deficient, recessive. The Mbpshi-mld mutation arose spontaneously in the MDB/Dt strain at Purdue University (J:5917). Homozygotes closely resemble Mbpshi/Mbpshi mice in behavior, neuropathology, and MBP deficiency(J:6320)(J:26989), and the two mutations were shown to be allelic by complementation test (18). In the Mbpshi-mld mutation the Mbp gene is duplicated (J:8798), and a large portion of the duplicated gene is inverted, leading to the formation ofantisense RNA (J:4643) which eliminates the mRNA for MBP (J:4161).The effects of the Mbpshi-mld mutation are less severe than those of Mbpshi. MBP is present in the CNS, though low in quantity, and the myelin deficient phenotype is rendered normal by an Mbp transgene (J:6320). Mbpshi/Mbpshi-mld heterozygotes are deficient in CNS myelin to a greater extent than Mbpshi-mld/Mbpshi-mld homozygotes, but less than Mbpshi/Mbpshi homozygotes (J:27481). Mbpshi-mld mice have normal lipid/protein ratios, lipid distribution patterns, and only moderate abnormalities of fatty acid profiles (J:27484), whereas these elements are strongly abnormal in Mbpshi (J:6012). Mbpshi caused more severe deficits in learning performance than Mbpshi-mld, while neither affected natural behavior (J:27482).Two stocks of Mbpshi-mld-bearing mice, both derived from the Purdue original stock at about the same time, and maintained respectively in Worcester, Massachusetts, and in Lausanne, Switzerland, show quantitative differences in life span, in incidence of convulsions, in MBP at older ages, and in proportion of optic axons myelinated. The differences are attributed to differences in regulatory genes in the two stocks, maintained on quite different backgrounds (J:27485).A revertant mutation of an Mbpshi-mld gene restored normal function to MBP. The revertant gene, +Mbp-shi-mld, was shown to consist of a single normal Mbp gene reconstituted from the 5' end of the upstream gene and the 3' end of the downstream gene in the Mbpshi-mld mutation (J:1873).

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Akowitz AA; Barbarese E; Scheld K; Carson JH. 1987. Structure and expression of myelin basic protein gene sequences in the mld mutant mouse: reiteration and rearrangement of the MBP gene. Genetics 116(3):447-64. [PubMed: 2440764]  [MGI Ref ID J:8798]

Chernoff GF. 1981. Shiverer: an autosomal recessive mutant mouse with myelin deficiency. J Hered 72(2):128. [PubMed: 6168677]  [MGI Ref ID J:6578]

Doolittle DP; Schweikart KM. 1977. Myelin deficient, a new neurological mutant in the mouse. J Hered 68(5):331-2. [PubMed: 599283]  [MGI Ref ID J:5917]

Matthieu JM; Ginalski H; Friede RL; Cohen SR; Doolittle DP. 1980. Absence of myelin basic protein and major dense line in CNS myelin of the mld mutant mouse. Brain Res 191(1):278-83. [PubMed: 6155168]  [MGI Ref ID J:6320]

Matthieu JM; Omlin FX; Ginalski-Winkelmann H; Cooper BJ. 1984. Myelination in the CNS of mld mutant mice: comparison between composition and structure. Brain Res 315(1):149-58. [PubMed: 6202376]  [MGI Ref ID J:26989]

Matthieu JM; Tosic M; Roach A. 1992. Myelin-deficient mutant mice. An in vivo model for inhibition of gene expression by natural antisense RNA. Ann N Y Acad Sci 660:188-92. [PubMed: 1285071]  [MGI Ref ID J:4161]

Nakajima K; Ikenaka K; Kagawa T; Aruga J; Nakao J; Nakahira K; Shiota C; Kim SU; Mikoshiba K. 1993. Novel isoforms of mouse myelin basic protein predominantly expressed in embryonic stage. J Neurochem 60(4):1554-63. [PubMed: 7681106]  [MGI Ref ID J:4643]

Roach A; Takahashi N; Pravtcheva D; Ruddle F; Hood L. 1985. Chromosomal mapping of mouse myelin basic protein gene and structure and transcription of the partially deleted gene in shiverer mutant mice. Cell 42(1):149-55. [PubMed: 2410137]  [MGI Ref ID J:7925]

Mbp^shi-mld related

Ainger K; Barbarese E; Berman L; Carson JH. 1992. Molecular genetic analysis of the mldr mouse: a spontaneous revertant at the mld locus containing a recombinant myelin basic protein gene. Genetics 130(2):367-75. [PubMed: 1371758]  [MGI Ref ID J:1873]

Akowitz A; Scheld K; Barbarese E; Carson JH. 1986. A partial duplication of the myelin basic protein gene in MLD mice Trans Am Soc Neurochem 17:108 (Abstr.).  [MGI Ref ID J:15385]

Akowitz AA; Barbarese E; Scheld K; Carson JH. 1987. Structure and expression of myelin basic protein gene sequences in the mld mutant mouse: reiteration and rearrangement of the MBP gene. Genetics 116(3):447-64. [PubMed: 2440764]  [MGI Ref ID J:8798]

Billings-Gagliardi S; Tosic M; Matthieu JM; Kirschner DA; Wolf MK. 1991. Quantitative differences between homozygous 'USA' and 'Swiss' mld mutant mice. Dev Neurosci 13(2):87-97. [PubMed: 1712720]  [MGI Ref ID J:27485]

Bird TD; Farrell DF; Sumi SM. 1978. Brain lipid composition of the shiverer mouse: (genetic defect in myelin development). J Neurochem 31(1):387-91. [PubMed: 671037]  [MGI Ref ID J:6012]

Cammer W; Zhang H; Tansey FA. 1995. Effects of carbonic anhydrase II (CAII) deficiency on CNS structure and function in the myelin-deficient CAII-deficient double mutant mouse. J Neurosci Res 40(4):451-7. [PubMed: 7616606]  [MGI Ref ID J:23601]

Campagnoni AT; Campagnoni CW; Bourre JM; Jacque C; Baumann N. 1984. Cell-free synthesis of myelin basic proteins in normal and dysmyelinating mutant mice. J Neurochem 42(3):733-9. [PubMed: 6198470]  [MGI Ref ID J:7310]

Doolittle DP; Schweikart KM. 1977. Myelin deficient, a new neurological mutant in the mouse. J Hered 68(5):331-2. [PubMed: 599283]  [MGI Ref ID J:5917]

Fressinaud C; Sarlieve LL; Cassagne C; Vincendon G; Matthieu JM. 1990. Normal composition of the major lipids and the distribution of their fatty acids in central nervous system myelin further discriminate mld from shiverer mice. Dev Neurosci 12(1):61-72. [PubMed: 1688761]  [MGI Ref ID J:27484]

Gould RM; Byrd AL; Barbarese E. 1995. The number of Schmidt-Lanterman incisures is more than doubled in shiverer PNS myelin sheaths. J Neurocytol 24(2):85-98. [PubMed: 7745445]  [MGI Ref ID J:27873]

Inagawa K; Watanabe S; Tsukada Y; Mikoshiba K. 1988. The role of myelination in learning performance observed in two strains of myelin-deficient mutant mice (shiverer and mld). Behav Neural Biol 50(2):184-92. [PubMed: 2465759]  [MGI Ref ID J:27482]

Inoue Y; Takahashi S; Takayama C; Inoue K; Okano H; Mikoshiba K. 1988. Central myelin in the first hybrid mice produced by intercrossing homozygotes of shiverer and myelin-deficient mutants. Brain Res 449(1-2):271-80. [PubMed: 3395848]  [MGI Ref ID J:27481]

Jacque C; Delassalle A; Raoul M; Baumann N. 1983. Myelin basic protein deposition in the optic and sciatic nerves of dysmyelinating mutants quaking, jimpy, Trembler, mld, and shiverer during development. J Neurochem 41(5):1335-40. [PubMed: 6194264]  [MGI Ref ID J:12030]

Kosaras B; Kirschner DA. 1990. Radial component of CNS myelin: junctional subunit structure and supramolecular assembly. J Neurocytol 19(2):187-99. [PubMed: 2113569]  [MGI Ref ID J:121320]

Matthieu JM; Amiguet P. 1990. Myelin/oligodendrocyte glycoprotein expression during development in normal and myelin-deficient mice. Dev Neurosci 12(4-5):293-302. [PubMed: 2289413]  [MGI Ref ID J:116787]

Matthieu JM; Ginalski H; Friede RL; Cohen SR; Doolittle DP. 1980. Absence of myelin basic protein and major dense line in CNS myelin of the mld mutant mouse. Brain Res 191(1):278-83. [PubMed: 6155168]  [MGI Ref ID J:6320]

Matthieu JM; Omlin FX; Ginalski-Winkelmann H; Cooper BJ. 1984. Myelination in the CNS of mld mutant mice: comparison between composition and structure. Brain Res 315(1):149-58. [PubMed: 6202376]  [MGI Ref ID J:26989]

Matthieu JM; Tosic M; Roach A. 1992. Myelin-deficient mutant mice. An in vivo model for inhibition of gene expression by natural antisense RNA. Ann N Y Acad Sci 660:188-92. [PubMed: 1285071]  [MGI Ref ID J:4161]

Nakajima K; Ikenaka K; Kagawa T; Aruga J; Nakao J; Nakahira K; Shiota C; Kim SU; Mikoshiba K. 1993. Novel isoforms of mouse myelin basic protein predominantly expressed in embryonic stage. J Neurochem 60(4):1554-63. [PubMed: 7681106]  [MGI Ref ID J:4643]

Okano H; Aruga J; Nakagawa T; Shiota C; Mikoshiba K. 1991. Myelin basic protein gene and the function of antisense RNA in its repression in myelin-deficient mutant mouse. J Neurochem 56(2):560-7. [PubMed: 1703220]  [MGI Ref ID J:10952]

Okano H; Miura M; Moriguchi A; Ikenaka K; Tsukada Y; Mikoshiba K. 1987. Inefficient transcription of the myelin basic protein gene possibly causes hypomyelination in myelin-deficient mutant mice. J Neurochem 48(2):470-6. [PubMed: 2432182]  [MGI Ref ID J:8535]

Okano H; Tamura T; Miura M; Aoyama A; Ikenaka K; Oshimura M; Mikoshiba K. 1988. Gene organization and transcription of duplicated MBP genes of myelin deficient (shi(mld)) mutant mouse. EMBO J 7(1):77-83. [PubMed: 2452084]  [MGI Ref ID J:9145]

Popko B; Puckett C; Hood L. 1988. A novel mutation in myelin-deficient mice results in unstable myelin basic protein gene transcripts. Neuron 1(3):221-5. [PubMed: 2483096]  [MGI Ref ID J:10329]

Popko B; Puckett C; Lai E; Shine HD; Readhead C; Takahashi N; Hunt SW 3d; Sidman RL; Hood L. 1987. Myelin deficient mice: expression of myelin basic protein and generation of mice with varying levels of myelin. Cell 48(4):713-21. [PubMed: 2434243]  [MGI Ref ID J:15364]

Readhead C; Hood L. 1990. The dysmyelinating mouse mutations shiverer (shi) and myelin deficient (shimld). Behav Genet 20(2):213-34. [PubMed: 1693848]  [MGI Ref ID J:27480]

Shine HD; Readhead C; Popko B; Hood L; Sidman RL. 1992. Morphometric analysis of normal, mutant, and transgenic CNS: correlation of myelin basic protein expression to myelinogenesis. J Neurochem 58(1):342-9. [PubMed: 1370079]  [MGI Ref ID J:1874]

Sun Y; Xu J; Barbarese E; Carson JH. 1997. Rearrangement and reactivation of the myelin basic protein locus in myelin-deficient (mld) mouse brain. J Neurochem 68(2):457-68. [PubMed: 9003030]  [MGI Ref ID J:37860]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.