Strain Name: |
B6;129S2-Cgatm1Sac/J |
|---|---|
Stock Number: |
002494 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Cga; Cgatm1Sac; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Sally Camper, University of Michigan Medical School Appearance
black
Related Genotype: a/aStrain Description
Mice homozygous for the Cgatm1Sac targeted mutation are viable but both sexes are infertile. They lack TSH, LH, and FSH. Homozygous mutant mice are hypogonadal and exhibit severe hypothyroidism resulting in dwarfism. Development of the thyroid gland was arrested in late gestation. However, gonadotropin releasing hormone (GNRH) neuron migration, development of secondary sex organs, and fetal and neonatal gonadal development are normal. Mice heterozygous for the Cgatm1Sac targeted mutation appear normal.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Cgatm1Sac | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Sally A Camper | ||
| Common Name(s) | TSHalpha -; | ||
| Mutation Made By | Sally Camper, University of Michigan Medical School | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | Cga, glycoprotein hormones, alpha subunit | ||
| Chromosome | 4 | ||
| Gene Common Name(s) | CG-ALPHA; FSHA; GPHA1; GPHa; GPHalpha; HCG; LHA; TSHA; Tsha; aGSU; alpha-GSU; alphaGSU; alphaSU; pituitary hormone; thyrotropin stimulating hormone, alpha subunit; | ||
| Molecular Note | A neomycin resistance gene was inserted into the third exon. In situ hybridization and RT-PCR experiments confirmed that the transcript was not present in pituitary of homozygous mice (data not shown). [MGI Ref ID J:28450] | ||
Control Information
| Allele | Control | |
|---|---|---|
| Cgatm1Sac | Wild-type from the colony | |
| Cgatm1Sac | 101045 B6129SF2/J | (approximate) |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Cgatm1Sac
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying other alleles of Cga
004426 B6;SJL-Tg(Cga-cre)3Sac/J View Strains carrying other alleles of Cga (1 strain)
Research Applications
This mouse can be used to support research in many areas including:Cgatm1Sac related
Developmental Biology Research
Growth Defects
Internal/Organ Defects (gonad, thyroid)
Endocrine Deficiency Research
Gonad Defects
Hypothalamus/Pituitary Defects
Thyroid Defects
Internal/Organ Research
Thyroid Defects
Reproductive Biology Research
Developmental Defects Affecting Gonads
Endocrine Deficiencies Affecting Gonads
Fertility Defects
References
Selected Reference(s)
Additional ReferencesKendall SK; Samuelson LC; Saunders TL; Wood RI; Camper SA. 1995. Targeted disruption of the pituitary glycoprotein hormone alpha-subunit produces hypogonadal and hypothyroid mice. Genes Dev 9(16):2007-19. [PubMed: 7544315] [MGI Ref ID J:28450]
Price and Supply Information
| Strain Name: | B6;129S2-Cgatm1Sac/J |
| Stock Number: | 002494 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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