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Strain Name:

B6;129S2-Cgatm1Sac/J

Stock Number:

002494

Availability:

Repository-Cryopreserved


General Terms and Conditions

Genes & Alleles   Cga;   Cgatm1Sac;


Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Targeted Mutation
Specieslaboratory mouse
Donating Investigator Sally Camper,   University of Michigan Medical School

Appearance
black
Related Genotype: a/a

Strain Description
Mice homozygous for the Cgatm1Sac targeted mutation are viable but both sexes are infertile. They lack TSH, LH, and FSH. Homozygous mutant mice are hypogonadal and exhibit severe hypothyroidism resulting in dwarfism. Development of the thyroid gland was arrested in late gestation. However, gonadotropin releasing hormone (GNRH) neuron migration, development of secondary sex organs, and fetal and neonatal gonadal development are normal. Mice heterozygous for the Cgatm1Sac targeted mutation appear normal.

Mammalian Phenotype Terms assigned by genotype

Cgatm1Sac/Cgatm1Sac

        involves: 129S2/SvPas * C57BL/6J
  • endocrine/exocrine gland phenotype
  • *normal* endocrine/exocrine gland phenotype (MGI Ref ID J:28450)
    • normal gonadotrope cellularity
    • abnormal corpus luteum (MGI Ref ID J:28450)
    • abnormal thyroid gland morphology (MGI Ref ID J:28450)
      • hypothyroid
      • abnormal thyroid follicle morphology (MGI Ref ID J:28450)
        • disorganized follicles
    • absent mature ovarian follicles (MGI Ref ID J:28450)
    • decreased lactotroph cell number (MGI Ref ID J:28450)
      • lactotropes are reduced in number in females
      • absent lactotrophs (MGI Ref ID J:28450)
        • lactotropes were undetected in males
    • decreased somatotroph cell number (MGI Ref ID J:28450)
      • reduced somatotrope cellularity
    • impaired ovarian folliculogenesis (MGI Ref ID J:28450)
      • follicles were reduced in size
      • no progress beyond the preantral stage
    • increased thyrotroph cell number (MGI Ref ID J:28450)
      • thyotrope hyperplasia and hypertrophy
    • small ovary (MGI Ref ID J:28450)
    • small seminal gland (MGI Ref ID J:28450)
      • seminal glands were not readily visible, consistent with testosterone deficiency
    • small seminiferous tubules (MGI Ref ID J:28450)
      • development of the tubules ceased perinatally
    • small testis (MGI Ref ID J:28450)
      • size reduction observed at 8 weeks of age
  • growth/size phenotype
  • decreased body size (MGI Ref ID J:28450)
    • mice were approximately half the size of wild type littermates at 4.5 weeks of age
    • decreased body weight (MGI Ref ID J:28450)
      • first evident between 2 and 3 weeks of age
    • dwarfism (MGI Ref ID J:28450)
  • postnatal growth retardation (MGI Ref ID J:28450)
    • no gender difference in size, putatively due to the decrease in testosterone levels
  • homeostasis/metabolism phenotype
  • decreased circulating follicle stimulating hormone level (MGI Ref ID J:28450)
  • decreased circulating luteinizing hormone level (MGI Ref ID J:28450)
  • decreased circulating testosterone level (MGI Ref ID J:28450)
    • serum testosterone was undetected in male mice
  • decreased circulating thyroid-stimulating hormone level (MGI Ref ID J:28450)
  • reproductive system phenotype
  • abnormal corpus luteum (MGI Ref ID J:28450)
  • abnormal ovulation (MGI Ref ID J:28450)
  • abnormal uterus morphology (MGI Ref ID J:28450)
    • underdeveloped and threadlike at 3 weeks of age
  • absent mature ovarian follicles (MGI Ref ID J:28450)
  • arrest of male meiosis (MGI Ref ID J:28450)
    • spermatogenesis was blocked at meiosis I
  • female infertility (MGI Ref ID J:28450)
  • fused vaginal opening (MGI Ref ID J:28450)
  • impaired ovarian folliculogenesis (MGI Ref ID J:28450)
    • follicles were reduced in size
    • no progress beyond the preantral stage
  • male infertility (MGI Ref ID J:28450)
  • small ovary (MGI Ref ID J:28450)
  • small seminal gland (MGI Ref ID J:28450)
    • seminal glands were not readily visible, consistent with testosterone deficiency
  • small seminiferous tubules (MGI Ref ID J:28450)
    • development of the tubules ceased perinatally
  • small testis (MGI Ref ID J:28450)
    • size reduction observed at 8 weeks of age
  • nervous system phenotype
  • decreased lactotroph cell number (MGI Ref ID J:28450)
    • lactotropes are reduced in number in females
    • absent lactotrophs (MGI Ref ID J:28450)
      • lactotropes were undetected in males
  • decreased somatotroph cell number (MGI Ref ID J:28450)
    • reduced somatotrope cellularity
  • increased thyrotroph cell number (MGI Ref ID J:28450)
    • thyotrope hyperplasia and hypertrophy

Gene & Allele Details

Allele Symbol Cgatm1Sac
Allele Name targeted mutation 1, Sally A Camper
Common Name(s) TSHalpha -;
Mutation Made By Sally Camper,   University of Michigan Medical School
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name Cga, glycoprotein hormones, alpha subunit
Chromosome 4
Gene Common Name(s) CG-ALPHA; FSHA; GPHA1; GPHa; GPHalpha; HCG; LHA; TSHA; Tsha; aGSU; alpha-GSU; alphaGSU; alphaSU; pituitary hormone; thyrotropin stimulating hormone, alpha subunit;
Molecular Note A neomycin resistance gene was inserted into the third exon. In situ hybridization and RT-PCR experiments confirmed that the transcript was not present in pituitary of homozygous mice (data not shown). [MGI Ref ID J:28450]

Control Information

  Allele   Control
 Cgatm1Sac  Wild-type from the colony
 Cgatm1Sac  101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Genotyping Protocols

Cgatm1Sac

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying other alleles of Cga
004426   B6;SJL-Tg(Cga-cre)3Sac/J
View Strains carrying other alleles of Cga     (1 strain)

Research Applications

This mouse can be used to support research in many areas including:

Cgatm1Sac related

Developmental Biology Research
Growth Defects
Internal/Organ Defects (gonad, thyroid)

Endocrine Deficiency Research
Gonad Defects
Hypothalamus/Pituitary Defects
Thyroid Defects

Internal/Organ Research
Thyroid Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads
Endocrine Deficiencies Affecting Gonads
Fertility Defects

References

Selected Reference(s)

Kendall SK; Samuelson LC; Saunders TL; Wood RI; Camper SA. 1995. Targeted disruption of the pituitary glycoprotein hormone alpha-subunit produces hypogonadal and hypothyroid mice. Genes Dev 9(16):2007-19. [PubMed: 7544315]  [MGI Ref ID J:28450]

Additional References

Price and Supply Information

Strain Name: B6;129S2-Cgatm1Sac/J
Stock Number: 002494

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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