Strain Name:

BALB/c-Il4tm2Nnt/J

Stock Number:

002496

Availability:

Repository- Live

Description

Strain Information

Type Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered Mutant Mice.
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
GenerationF?+28 (05-DEC-07)

Appearance
albino
Related Genotype: A/A Tyrp1b/Tyrp1b Tyrc/Tyrc

Description
Mice homozygous for the Il4tm2Nnt targeted mutation are viable and fertile. T and B cell development is normal but IgGl and IgE levels and the ability of homozygous mutant mice to produce Th2-derived cytokines are significantly reduced. Susceptible to infection by Leishmania major despite deficiency in IL4.

Development
This strain was developed by Dr. Nancy Noben-Trauth and Dr. Birgit Ledermann at Sandoz Pharma Ltd. in Basel, Switzerland. A BALB/c-derived ES cell line was used.

Control Information

  Control
   000651 BALB/cJ
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Il4     (11 strains)

Additional Web Information

Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Il4tm2Nnt/Il4tm2Nnt

        BALB/c-Il4tm2Nnt/J
  • immune system phenotype
  • decreased IgE level (MGI Ref ID J:31486)
    • reduced serum concentrations of IgE, however lymphocyte composition and function comparable to wild-type controls
  • decreased IgG1 level (MGI Ref ID J:31486)
    • reduced serum concentrations of IgG1, however lymphocyte composition and function comparable to wild-type controls
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Il4tm2Nnt related

Cancer Research
Growth Factors/Receptors/Cytokines

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines

Genes & Alleles

Gene & Allele Information

Allele Symbol Il4tm2Nnt
Allele Name targeted mutation 2, Nancy Noben-Trauth
Allele Type Targeted (knock-out)
Common Name(s) IL-4-;
Mutation Made By Nancy Noben-Trauth,  
Strain of OriginBALB/cJ
ES Cell Line NameBALB/c-I
ES Cell Line StrainBALB/cJ
Gene Symbol and Name Il4, interleukin 4
Chromosome 11
Gene Common Name(s) BCGF-1; BCGF1; BSF1; IL-4; Il-4; Il4e12; MGC79402;
General Note Susceptibility to Leishmania major in BALB/c mice has been ablated by introduction of a null mutant of the Il4 gene (J:35236), but in that study the introduced mutant came from a strain 129 background, and 129 is resistant to L. major infection. In mice carrying one of Noben-Trauth's null mutants, targeted in BALB/c embryonic stem cells, the loss of Il4 expression did not remove susceptibility to infection (J:31486).
Molecular Note A neomycin resistance cassette was inserted into exon 3 of the gene. [MGI Ref ID J:36386]

Genotyping

Genotyping Information

Genotyping Protocols

Il4tm2Nnt, SEP PCR, vers. 1
Il4tm2Nnt, SEP PCR, vers. 2

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Noben-Trauth N; Kohler G; Burki K; Ledermann B. 1996. Efficient targeting of the IL-4 gene in a BALB/c embryonic stem cell line. Transgenic Res 5(6):487-91. [PubMed: 8840532]  [MGI Ref ID J:36386]

Additional References

Czarneski J; Meyers J; Peng T; Abraham V; Mick R; Ross SR. 2001. Interleukin-4 Up-Regulates Mouse Mammary Tumor Virus Expression yet Is Not Required for In Vivo Virus Spread. J Virol 75(23):11886-90. [PubMed: 11689671]  [MGI Ref ID J:73032]

Dohi T; Fujihashi K; Kiyono H; Elson CO; McGhee JR. 2000. Mice deficient in Th1- and Th2-type cytokines develop distinct forms of hapten-induced colitis. Gastroenterology 119(3):724-33. [PubMed: 10982767]  [MGI Ref ID J:64230]

Hall LR; Berger RB; Diaconu E; Pearlman E. 2002. Onchocerca volvulus keratitis (river blindness) is exacerbated in BALB/c IL-4 gene knockout mice. Cell Immunol 216(1-2):1-5. [PubMed: 12381344]  [MGI Ref ID J:80510]

Hata H; Sakaguchi N; Yoshitomi H; Iwakura Y; Sekikawa K; Azuma Y; Kanai C; Moriizumi E; Nomura T; Nakamura T; Sakaguchi S. 2004. Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice. J Clin Invest 114(4):582-8. [PubMed: 15314695]  [MGI Ref ID J:92002]

Kodera T; McGaha TL; Phelps R; Paul WE; Bona CA. 2002. Disrupting the IL-4 gene rescues mice homozygous for the tight-skin mutation from embryonic death and diminishes TGF-beta production by fibroblasts. Proc Natl Acad Sci U S A 99(6):3800-5. [PubMed: 11891315]  [MGI Ref ID J:75471]

Kolodsick JE; Toews GB; Jakubzick C; Hogaboam C; Moore TA; McKenzie A; Wilke CA; Chrisman CJ; Moore BB. 2004. Protection from fluorescein isothiocyanate-induced fibrosis in IL-13-deficient, but not IL-4-deficient, mice results from impaired collagen synthesis by fibroblasts. J Immunol 172(7):4068-76. [PubMed: 15034018]  [MGI Ref ID J:88681]

Li X; Wang Y; Urso D; O'Rourke J; Cone RE. 2004. Thymocytes induced by antigen injection into the anterior chamber activate splenic CD8+ suppressor cells and enhance the antigen-induced production of immunoglobulin G1 antibodies. Immunology 113(1):44-56. [PubMed: 15312135]  [MGI Ref ID J:92936]

Noben-Trauth N; Kropf P; Muller I. 1996. Susceptibility to Leishmania major infection in interleukin-4-deficient mice [see comments] Science 271(5251):987-90. [PubMed: 8584936]  [MGI Ref ID J:31486]

Spencer L; Shultz L; Rajan TV. 2001. Interleukin-4 receptor-stat6 signaling in murine infections with a tissue-dwelling nematode parasite. Infect Immun 69(12):7743-52. [PubMed: 11705956]  [MGI Ref ID J:73137]

Woolard MD; Hodge LM; Jones HP; Schoeb TR; Simecka JW. 2004. The upper and lower respiratory tracts differ in their requirement of IFN-gamma and IL-4 in controlling respiratory mycoplasma infection and disease. J Immunol 172(11):6875-83. [PubMed: 15153506]  [MGI Ref ID J:90522]

Il4tm2Nnt related

Alexander J; Carter KC; Al-Fasi N; Satoskar A; Brombacher F. 2000. Endogenous IL-4 is necessary for effective drug therapy against visceral leishmaniasis Eur J Immunol 30(10):2935-43. [PubMed: 11069076]  [MGI Ref ID J:65232]

Andoh A; Masuda A; Kumazawa Y; Kasajima T. 2002. Serum antibody response and nasal lymphoid tissue (NALT) structure in the absence of IL-4 or IFN-gamma. Cytokine 20(3):107-12. [PubMed: 12453468]  [MGI Ref ID J:102594]

Artis D; Humphreys NE; Bancroft AJ; Rothwell NJ; Potten CS; Grencis RK. 1999. Tumor necrosis factor alpha is a critical component of interleukin 13-mediated protective T helper cell type 2 responses during helminth infection. J Exp Med 190(7):953-62. [PubMed: 10510085]  [MGI Ref ID J:115088]

Belkaid Y; Mendez S; Lira R; Kadambi N; Milon G; Sacks D. 2000. A natural model of Leishmania major infection reveals a prolonged 'silent' phase of parasite amplification in the skin before the onset of lesion formation and immunity. J Immunol 165(2):969-77. [PubMed: 10878373]  [MGI Ref ID J:120233]

Bian K; Zhong M; Harari Y; Lai M; Weisbrodt N; Murad F. 2005. Helminth regulation of host IL-4Ralpha/Stat6 signaling: mechanism underlying NOS-2 inhibition by Trichinella spiralis. Proc Natl Acad Sci U S A 102(11):3936-41. [PubMed: 15741272]  [MGI Ref ID J:97167]

Brewer JM; Conacher M; Hunter CA; Mohrs M; Brombacher F; Alexander J. 1999. Aluminium hydroxide adjuvant initiates strong antigen-specific Th2 responses in the absence of IL-4- or IL-13-mediated signaling. J Immunol 163(12):6448-54. [PubMed: 10586035]  [MGI Ref ID J:58976]

Castilow EM; Meyerholz DK; Varga SM. 2008. IL-13 is required for eosinophil entry into the lung during respiratory syncytial virus vaccine-enhanced disease. J Immunol 180(4):2376-84. [PubMed: 18250447]  [MGI Ref ID J:131994]

Cohn L; Herrick C; Niu N; Homer R; Bottomly K. 2001. IL-4 promotes airway eosinophilia by suppressing IFN-gamma production: defining a novel role for IFN-gamma in the regulation of allergic airway inflammation. J Immunol 166(4):2760-7. [PubMed: 11160342]  [MGI Ref ID J:126916]

Dogan RN; Vasu C; Holterman MJ; Prabhakar BS. 2003. Absence of IL-4, and not suppression of the Th2 response, prevents development of experimental autoimmune Graves' disease. J Immunol 170(4):2195-204. [PubMed: 12574393]  [MGI Ref ID J:126279]

Dohi T; Fujihashi K; Kiyono H; Elson CO; McGhee JR. 2000. Mice deficient in Th1- and Th2-type cytokines develop distinct forms of hapten-induced colitis. Gastroenterology 119(3):724-33. [PubMed: 10982767]  [MGI Ref ID J:64230]

Dohi T; Fujihashi K; Rennert PD; Iwatani K; Kiyono H; McGhee JR. 1999. Hapten-induced colitis is associated with colonic patch hypertrophy and T helper cell 2-type responses. J Exp Med 189(8):1169-80. [PubMed: 10209035]  [MGI Ref ID J:119172]

Fallon PG; Richardson EJ; McKenzie GJ; McKenzie AN. 2000. Schistosome infection of transgenic mice defines distinct and contrasting pathogenic roles for IL-4 and IL-13: IL-13 is a profibrotic agent. J Immunol 164(5):2585-91. [PubMed: 10679097]  [MGI Ref ID J:60609]

Finnegan A; Grusby MJ; Kaplan CD; O'Neill SK; Eibel H; Koreny T; Czipri M; Mikecz K; Zhang J. 2002. IL-4 and IL-12 regulate proteoglycan-induced arthritis through Stat-dependent mechanisms. J Immunol 169(6):3345-52. [PubMed: 12218156]  [MGI Ref ID J:120443]

Fish SC; Donaldson DD; Goldman SJ; Williams CM; Kasaian MT. 2005. IgE generation and mast cell effector function in mice deficient in IL-4 and IL-13. J Immunol 174(12):7716-24. [PubMed: 15944273]  [MGI Ref ID J:100883]

Fujita K; Kobayashi M; Brutkiewicz RR; Hanafusa T; Herndon DN; Suzuki F. 2006. Role for IL-4 nonproducing NKT cells in CC-chemokine ligand 2-induced Th2 cell generation. Immunol Cell Biol 84(1):44-50. [PubMed: 16277637]  [MGI Ref ID J:105853]

Gallina G; Dolcetti L; Serafini P; De Santo C; Marigo I; Colombo MP; Basso G; Brombacher F; Borrello I; Zanovello P; Bicciato S; Bronte V. 2006. Tumors induce a subset of inflammatory monocytes with immunosuppressive activity on CD8+ T cells. J Clin Invest 116(10):2777-90. [PubMed: 17016559]  [MGI Ref ID J:114986]

Ghassabeh GH; De Baetselier P; Brys L; Noel W; Van Ginderachter JA; Meerschaut S; Beschin A; Brombacher F; Raes G. 2006. Identification of a common gene signature for type II cytokine-associated myeloid cells elicited in vivo in different pathologic conditions. Blood 108(2):575-83. [PubMed: 16556895]  [MGI Ref ID J:135755]

Gorham JD; Lin JT; Sung JL; Rudner LA; French MA. 2001. Genetic regulation of autoimmune disease: balb/c background tgf-beta1-deficient mice develop necroinflammatory ifn-gamma-dependent hepatitis. J Immunol 166(10):6413-22. [PubMed: 11342667]  [MGI Ref ID J:69346]

Hata H; Sakaguchi N; Yoshitomi H; Iwakura Y; Sekikawa K; Azuma Y; Kanai C; Moriizumi E; Nomura T; Nakamura T; Sakaguchi S. 2004. Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice. J Clin Invest 114(4):582-8. [PubMed: 15314695]  [MGI Ref ID J:92002]

Heinzel FP; Maier RA Jr. 1999. Interleukin-4-independent acceleration of cutaneous leishmaniasis in susceptible BALB/c mice following treatment with anti-CTLA4 antibody. Infect Immun 67(12):6454-60. [PubMed: 10569763]  [MGI Ref ID J:58540]

Hernandez-Pando R; Aguilar D; Hernandez ML; Orozco H; Rook G. 2004. Pulmonary tuberculosis in BALB/c mice with non-functional IL-4 genes: changes in the inflammatory effects of TNF-alpha and in the regulation of fibrosis. Eur J Immunol 34(1):174-83. [PubMed: 14971043]  [MGI Ref ID J:87666]

Herold KC; Lu J; Rulifson I; Vezys V; Taub D; Grusby MJ; Bluestone JA. 1997. Regulation of C-C chemokine production by murine T cells by CD28/B7 costimulation. J Immunol 159(9):4150-3. [PubMed: 9379007]  [MGI Ref ID J:110673]

Herrick CA; MacLeod H; Glusac E; Tigelaar RE; Bottomly K. 2000. Th2 responses induced by epicutaneous or inhalational protein exposure are differentially dependent on IL-4. J Clin Invest 105(6):765-75. [PubMed: 10727445]  [MGI Ref ID J:61145]

Hiraoka S; Takeuchi N; Bian Y; Nakahara H; Kogo M; Dunussi-Joannopoulos K; Wolf S; Ono S; Fujiwara H. 2005. B7.2-Ig fusion proteins enhance IL-4-dependent differentiation of tumor-sensitized CD8+ cytotoxic T lymphocyte precursors. Int Immunol 17(8):1071-9. [PubMed: 16027141]  [MGI Ref ID J:99968]

Hogan SP; Matthaei KI; Young JM; Koskinen A; Young IG; Foster PS. 1998. A novel T cell-regulated mechanism modulating allergen-induced airways hyperreactivity in BALB/c mice independently of IL-4 and IL-5. J Immunol 161(3):1501-9. [PubMed: 9686617]  [MGI Ref ID J:48882]

Inoue Y; Konieczny BT; Wagener ME; McKenzie AN; Lakkis FG. 2001. Failure to induce neonatal tolerance in mice that lack both IL-4 and IL-13 but not in those that lack IL-4 alone. J Immunol 167(2):1125-8. [PubMed: 11441125]  [MGI Ref ID J:106687]

Jessup HK; Brewer AW; Omori M; Rickel EA; Budelsky AL; Yoon BR; Ziegler SF; Comeau MR. 2008. Intradermal administration of thymic stromal lymphopoietin induces a T cell- and eosinophil-dependent systemic Th2 inflammatory response. J Immunol 181(6):4311-9. [PubMed: 18768889]  [MGI Ref ID J:139079]

Johnson JR; Swirski FK; Gajewska BU; Wiley RE; Fattouh R; Pacitto SR; Wong JK; Stampfli MR; Jordana M. 2007. Divergent immune responses to house dust mite lead to distinct structural-functional phenotypes. Am J Physiol Lung Cell Mol Physiol 293(3):L730-9. [PubMed: 17586699]  [MGI Ref ID J:128039]

Kodera T; McGaha TL; Phelps R; Paul WE; Bona CA. 2002. Disrupting the IL-4 gene rescues mice homozygous for the tight-skin mutation from embryonic death and diminishes TGF-beta production by fibroblasts. Proc Natl Acad Sci U S A 99(6):3800-5. [PubMed: 11891315]  [MGI Ref ID J:75471]

Kolodsick JE; Toews GB; Jakubzick C; Hogaboam C; Moore TA; McKenzie A; Wilke CA; Chrisman CJ; Moore BB. 2004. Protection from fluorescein isothiocyanate-induced fibrosis in IL-13-deficient, but not IL-4-deficient, mice results from impaired collagen synthesis by fibroblasts. J Immunol 172(7):4068-76. [PubMed: 15034018]  [MGI Ref ID J:88681]

Komine O; Hayashi K; Natsume W; Watanabe T; Seki Y; Seki N; Yagi R; Sukzuki W; Tamauchi H; Hozumi K; Habu S; Kubo M; Satake M. 2003. The Runx1 Transcription Factor Inhibits the Differentiation of Naive CD4+ T Cells into the Th2 Lineage by Repressing GATA3 Expression. J Exp Med 198(1):51-61. [PubMed: 12835475]  [MGI Ref ID J:84459]

Kopf M; Brombacher F; Kohler G; Kienzle G; Widmann KH; Lefrang K; Humborg C; Ledermann B; Solbach W. 1996. IL-4-deficient Balb/c mice resist infection with Leishmania major. J Exp Med 184(3):1127-36. [PubMed: 9064329]  [MGI Ref ID J:35236]

Kropf P; Schopf LR; Chung CL; Xu D; Liew FY; Sypek JP; Muller I. 1999. Expression of Th2 cytokines and the stable Th2 marker ST2L in the absence of IL-4 during Leishmania major infection. Eur J Immunol 29(11):3621-8. [PubMed: 10556817]  [MGI Ref ID J:115123]

Mangashetti LS; Khapli SM; Wani MR. 2005. IL-4 inhibits bone-resorbing activity of mature osteoclasts by affecting NF-kappa B and Ca2+ signaling. J Immunol 175(2):917-25. [PubMed: 16002690]  [MGI Ref ID J:100700]

Maroof A; Penny M; Kingston R; Murray C; Islam S; Bedford PA; Knight SC. 2006. Interleukin-4 can induce interleukin-4 production in dendritic cells. Immunology 117(2):271-9. [PubMed: 16423063]  [MGI Ref ID J:106772]

Metwali A; Elliott D; Blum AM; Li J; Sandor M; Lynch R; Noben-Trauth N; Weinstock JV. 1996. The granulomatous response in murine Schistosomiasis mansoni does not switch to Th1 in IL-4-deficient C57BL/6 mice. J Immunol 157(10):4546-53. [PubMed: 8906833]  [MGI Ref ID J:37196]

Mirmonsef P; Shelburne CP; Fitzhugh Yeatman C 2nd; Chong HJ; Ryan JJ. 1999. Inhibition of Kit expression by IL-4 and IL-10 in murine mast cells: role of STAT6 and phosphatidylinositol 3'-kinase. J Immunol 163(5):2530-9. [PubMed: 10452990]  [MGI Ref ID J:57096]

Mohrs M; Ledermann B; Kohler G; Dorfmuller A; Gessner A; Brombacher F. 1999. Differences between IL-4- and IL-4 receptor alpha-deficient mice in chronic leishmaniasis reveal a protective role for IL-13 receptor signaling. J Immunol 162(12):7302-8. [PubMed: 10358179]  [MGI Ref ID J:55526]

Morimoto Y; Bian Y; Gao P; Yashiro-Ohtani Y; Zhou XY; Ono S; Nakahara H; Kogo M; Hamaoka T; Fujiwara H. 2005. Induction of surface CCR4 and its functionality in mouse Th2 cells is regulated differently during Th2 development. J Leukoc Biol 78(3):753-61. [PubMed: 16126843]  [MGI Ref ID J:100501]

Nagayama Y; Saitoh O; McLachlan SM; Rapoport B; Kano H; Kumazawa Y. 2004. TSH receptor-adenovirus-induced Graves' hyperthyroidism is attenuated in both interferon-gamma and interleukin-4 knockout mice; implications for the Th1/Th2 paradigm. Clin Exp Immunol 138(3):417-22. [PubMed: 15544617]  [MGI Ref ID J:94577]

Noben-Trauth N. 2000. Susceptibility to Leishmania major infection in the absence of IL-4. Immunol Lett 75(1):41-4. [PubMed: 11163865]  [MGI Ref ID J:66151]

Noben-Trauth N; Hu-Li J; Paul WE. 2000. Conventional, naive CD4+ T cells provide an initial source of IL-4 during Th2 differentiation. J Immunol 165(7):3620-5. [PubMed: 11034364]  [MGI Ref ID J:119587]

Noben-Trauth N; Kropf P; Muller I. 1996. Susceptibility to Leishmania major infection in interleukin-4-deficient mice [see comments] Science 271(5251):987-90. [PubMed: 8584936]  [MGI Ref ID J:31486]

Osawa E; Nakajima A; Fujisawa T; Kawamura YI; Toyama-Sorimachi N; Nakagama H; Dohi T. 2006. Predominant T helper type 2-inflammatory responses promote murine colon cancers. Int J Cancer 118(9):2232-6. [PubMed: 16331625]  [MGI Ref ID J:107259]

Panthel K; Faller G; Haas R. 2003. Colonization of C57BL/6J and BALB/c wild-type and knockout mice with Helicobacter pylori: effect of vaccination and implications for innate and acquired immunity. Infect Immun 71(2):794-800. [PubMed: 12540559]  [MGI Ref ID J:81703]

Petritus PM; Burns JM Jr. 2008. Suppression of lethal Plasmodium yoelii malaria following protective immunization requires antibody-, IL-4-, and IFN-gamma-dependent responses induced by vaccination and/or challenge infection. J Immunol 180(1):444-53. [PubMed: 18097046]  [MGI Ref ID J:130916]

Potter MR; Noben-Trauth N; Weis JH; Teuscher C; Weis JJ. 2000. Interleukin-4 (IL-4) and IL-13 signaling pathways Do not regulate borrelia burgdorferi-induced arthritis in mice: IgG1 is not required for host control of tissue spirochetes Infect Immun 68(10):5603-9. [PubMed: 10992460]  [MGI Ref ID J:64838]

Sasaki H; Hou L; Belani A; Wang CY; Uchiyama T; Muller R; Stashenko P. 2000. IL-10, but not IL-4, suppresses infection-stimulated bone resorption in vivo. J Immunol 165(7):3626-30. [PubMed: 11034365]  [MGI Ref ID J:118728]

Schuler T; Qin Z; Ibe S; Noben-Trauth N; Blankenstein T. 1999. T helper cell type 1-associated and cytotoxic T lymphocyte-mediated tumor immunity is impaired in interleukin 4-deficient mice. J Exp Med 189(5):803-10. [PubMed: 10049944]  [MGI Ref ID J:53801]

Schwarze J; Cieslewicz G; Hamelmann E; Joetham A; Shultz LD; Lamers MC; Gelfand EW. 1999. IL-5 and eosinophils are essential for the development of airway hyperresponsiveness following acute respiratory syncytial virus infection. J Immunol 162(5):2997-3004. [PubMed: 10072551]  [MGI Ref ID J:110844]

Shoemaker J; Saraiva M; O'Garra A. 2006. GATA-3 directly remodels the IL-10 locus independently of IL-4 in CD4+ T cells. J Immunol 176(6):3470-9. [PubMed: 16517715]  [MGI Ref ID J:129513]

Singh NJ; Chen C; Schwartz RH. 2006. The impact of T cell intrinsic antigen adaptation on peripheral immune tolerance. PLoS Biol 4(11):e340. [PubMed: 17048986]  [MGI Ref ID J:116094]

Sokol CL; Barton GM; Farr AG; Medzhitov R. 2008. A mechanism for the initiation of allergen-induced T helper type 2 responses. Nat Immunol 9(3):310-8. [PubMed: 18300366]  [MGI Ref ID J:131552]

Spencer L; Shultz L; Rajan TV. 2001. Interleukin-4 receptor-stat6 signaling in murine infections with a tissue-dwelling nematode parasite. Infect Immun 69(12):7743-52. [PubMed: 11705956]  [MGI Ref ID J:73137]

Spergel JM; Mizoguchi E; Oettgen H; Bhan AK; Geha RS. 1999. Roles of TH1 and TH2 cytokines in a murine model of allergic dermatitis. J Clin Invest 103(8):1103-11. [PubMed: 10207161]  [MGI Ref ID J:119611]

Tawara I; Maeda Y; Sun Y; Lowler KP; Liu C; Toubai T; McKenzie AN; Reddy P. 2008. Combined Th2 cytokine deficiency in donor T cells aggravates experimental acute graft-vs-host disease. Exp Hematol 36(8):988-96. [PubMed: 18410989]  [MGI Ref ID J:139377]

Yokota N; Burne-Taney M; Racusen L; Rabb H. 2003. Contrasting roles for STAT4 and STAT6 signal transduction pathways in murine renal ischemia-reperfusion injury. Am J Physiol Renal Physiol 285(2):F319-25. [PubMed: 12709397]  [MGI Ref ID J:84825]

Yoshimoto T; Min B; Sugimoto T; Hayashi N; Ishikawa Y; Sasaki Y; Hata H; Takeda K; Okumura K; Van Kaer L; Paul WE; Nakanishi K. 2003. Nonredundant roles for CD1d-restricted natural killer T cells and conventional CD4+ T cells in the induction of immunoglobulin E antibodies in response to interleukin 18 treatment of mice. J Exp Med 197(8):997-1005. [PubMed: 12695491]  [MGI Ref ID J:120694]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & HusbandryThe strain is maintained by homozygous sibling matings. Expected coat color from breeding:Albino
Mating SystemHomozygote x Homozygote         (Female x Male)
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*GenderGenotypes Provided
Individual Mouse Price $81.60Female or MaleHomozygous for Il4tm2Nnt
Pairs /Price*Pair Genotype
$163.20Homozygous for Il4tm2Nnt x Homozygous for Il4tm2Nnt
*Price(s) in US dollars ($)

Additional Supply Details

Supply Notes

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*GenderGenotypes Provided
Individual Mouse Price $106.10Female or MaleHomozygous for Il4tm2Nnt
Pairs /Price*Pair Genotype
$212.20Homozygous for Il4tm2Nnt x Homozygous for Il4tm2Nnt
*Price(s) in US dollars ($)

Additional Supply Details

Supply Notes

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes

Control Information

  Control
   000651 BALB/cJ
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


See Terms of Use


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use


General Terms and Conditions


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phone:207-288-6470
fax:207-288-6655

JAX® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. THE LABORATORY EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.


(3.2)