| |||||||||
Former Names 129S4/SvJae-Ntf5tm1Jae/J (Changed: 07-SEP-06 ) Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Donating Investigator Rudolf Jaenisch, Massachusetts Institute of Technology Appearance
white-bellied agouti
Related Genotype: Aw/AwDescription
Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice lose sensory neurons in the nodose-petrosal and geniculate ganglia. However, they do not show loss of facial nucleus motor neurons, sympathetic neurons of the superior cervical ganglion, or dopaminergic neurons in the substantia nigra.Development
This strain was developed in the lab of Dr. Rudolph Jaenisch at the Whitehead Institute for Biomedical Research at the Massachusetts Institute of Technology. A targeting vector containing neomycin resistance and phosphoglycerate kinase genes was used to disrupt the entire coding sequence. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129T1/Sv-p+ Tyrc-ch Dnd1Ter mice. The mice were then crossed to 129S1/SvImJ (Stock No. 2448) for cryopreservation.
| Control | ||
|---|---|---|
| 002448 129S1/SvImJ | (approximate) | |
| Considerations for Choosing Controls | ||
New 129 Nomenclature Bulletin
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Ntf5tm1Jae/Ntf5tm1Jae
involves: 129S4/SvJae
- nervous system phenotype
- decreased neuron apoptosis (MGI Ref ID J:123022)
- at E12 and E14, neuron apoptosis is reduced 74% and 65%, respectively, compared to in wild-type mice
- decreased sensory neuron number (MGI Ref ID J:25566)
- significant loss of all sensory neuron types in the nodose-petrosal, geniculate and L4 dorsal root ganglion in homozygous mice
- no difference in the number of facial motor neurons or superior cervical ganglion neurons is seen compared to controls
- no difference in the number of midbrain dopaminergic neurons is seen compared to controls
- small L4 dorsal root ganglion (MGI Ref ID J:25566)
- approximately 15% of the neurons are lost from the L4 DRG at two months of age
- small geniculate ganglion (MGI Ref ID J:25566)
- approximately half of the neurons are lost from the geniculate ganglion at E18.5
- small nodose ganglion (MGI Ref ID J:25566)
- greater than half of the neurons are lost from the nodose-petrosal ganglion at E18.5
- small petrosal ganglion (MGI Ref ID J:25566)
- greater than half of the neurons are lost from the nodose-petrosal ganglion at E18.5
- small vestibular ganglion (MGI Ref ID J:25566)
- approximately 20% of the neurons are lost from the vestibular ganglion
- skin/coat/nails phenotype
- abnormal hair cycle (MGI Ref ID J:53271)
- retarded catagen progression; follicles are still in early or middle catagen at time points when controls are in late catagen
- thick skin (MGI Ref ID J:53271)
- skin thicker than in controls
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:Ntf5tm1Jae related
Apoptosis Research
Extracellular Modulators
Cancer Research
Growth Factors/Receptors/Cytokines
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Neurobiology Research
Neurotrophic Factor Defects
| Allele Symbol | Ntf5tm1Jae | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Rudolf Jaenisch | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | NT-4 -; NT4; | ||
| Mutation Made By | Rudolf Jaenisch, Massachusetts Institute of Technology | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Ntf5, neurotrophin 5 | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | 2900040K06Rik; AI462899; NT-4; NT-4/5; NT4; NT4/5; NT4P; NT5; Ntf-5; Ntf4; RIKEN cDNA 2900040K06 gene; expressed sequence AI462899; neurotrophin 4; neurotrophin-4; | ||
| Molecular Note | The entire coding region of the gene was replaced by a PGK-neomycin cassette. [MGI Ref ID J:25566] | ||
Genotyping Protocols
Ntf5tm1Jae, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
Liu X; Ernfors P; Wu H; Jaenisch R. 1995. Sensory but not motor neuron deficits in mice lacking NT4 and BDNF. Nature 375(6528):238-41. [PubMed: 7746325] [MGI Ref ID J:25566]
Fox EA; Phillips RJ; Baronowsky EA; Byerly MS; Jones S; Powley TL. 2001. Neurotrophin-4 deficient mice have a loss of vagal intraganglionic mechanoreceptors from the small intestine and a disruption of short-term satiety. J Neurosci 21(21):8602-15. [PubMed: 11606648] [MGI Ref ID J:72356]
Stucky CL; Shin JB; Lewin GR. 2002. Neurotrophin-4: a survival factor for adult sensory neurons. Curr Biol 12(16):1401-4. [PubMed: 12194821] [MGI Ref ID J:78592]
Ntf5tm1Jae relatedAgerman K; Baudet C; Fundin B; Willson C; Ernfors P. 2000. Attenuation of a caspase-3 dependent cell death in NT4- and p75-deficient embryonic sensory neurons. Mol Cell Neurosci 16(3):258-68. [PubMed: 10995552] [MGI Ref ID J:123022]
Botchkarev VA; Botchkareva NV; Welker P; Metz M; Lewin GR; Subramaniam A; Bulfone-Paus S; Hagen E; Braun A; Lommatzsch M; Renz H; Paus AR. 1999. A new role for neurotrophins: involvement of brain-derived neurotrophic factor and neurotrophin-4 in hair cycle control. FASEB J 13(2):395-410. [PubMed: 9973328] [MGI Ref ID J:53271]
Botchkarev VA; Metz M; Botchkareva NV; Welker P; Lommatzsch M; Renz H; Paus R. 1999. Brain-derived neurotrophic factor, neurotrophin-3, and neurotrophin-4 act as epitheliotrophins in murine skin. Lab Invest 79(5):557-72. [PubMed: 10334567] [MGI Ref ID J:54992]
Byerly MS; Fox EA. 2006. High-fat hyperphagia in neurotrophin-4 deficient mice reveals potential role of vagal intestinal sensory innervation in long-term controls of food intake. Neurosci Lett 400(3):240-5. [PubMed: 16530962] [MGI Ref ID J:111211]
Calamusa M; Pattabiraman PP; Pozdeyev N; Iuvone PM; Cellerino A; Domenici L. 2007. Specific alterations of tyrosine hydroxylase immunopositive cells in the retina of NT-4 knock out mice. Vision Res 47(11):1523-36. [PubMed: 17350071] [MGI Ref ID J:124475]
ElShamy WM; Ernfors P. 1997. Brain-derived neurotrophic factor, neurotrophin-3, and neurotrophin-4 complement and cooperate with each other sequentially during visceral neuron development. J Neurosci 17(22):8667-75. [PubMed: 9348335] [MGI Ref ID J:44086]
Endres M; Fan G; Hirt L; Fujii M; Matsushita K; Liu X; Jaenisch R; Moskowitz MA. 2000. Ischemic brain damage in mice after selectively modifying BDNF or NT4 gene expression. J Cereb Blood Flow Metab 20(1):139-44. [PubMed: 10616802] [MGI Ref ID J:60087]
English AW; Meador W; Carrasco DI. 2005. Neurotrophin-4/5 is required for the early growth of regenerating axons in peripheral nerves. Eur J Neurosci 21(10):2624-34. [PubMed: 15926911] [MGI Ref ID J:101068]
Fan G; Copray S; Huang EJ; Jones K; Yan Q; Walro J; Jaenisch R; Kucera J. 2000. Formation of a full complement of cranial proprioceptors requires multiple neurotrophins. Dev Dyn 218(2):359-70. [PubMed: 10842362] [MGI Ref ID J:62766]
Fox EA; Phillips RJ; Baronowsky EA; Byerly MS; Jones S; Powley TL. 2001. Neurotrophin-4 deficient mice have a loss of vagal intraganglionic mechanoreceptors from the small intestine and a disruption of short-term satiety. J Neurosci 21(21):8602-15. [PubMed: 11606648] [MGI Ref ID J:72356]
Fundin BT; Silos-Santiago I; Ernfors P; Fagan AM; Aldskogius H ; DeChiara TM ; Phillips HS ; Barbacid M ; Yancopoulos GD ; Rice FL. 1997. Differential dependency of cutaneous mechanoreceptors on neurotrophins, trk receptors, and P75 LNGFR. Dev Biol 190(1):94-116. [PubMed: 9331334] [MGI Ref ID J:43425]
Gacek RR; Khetarpal U. 1998. Neurotrophin 3, not brain-derived neurotrophic factor or neurotrophin 4, knockout mice have delay in vestibular compensation after unilateral labyrinthectomy. Laryngoscope 108(5):671-8. [PubMed: 9591544] [MGI Ref ID J:113175]
He XP; Butler L; Liu X; McNamara JO. 2006. The tyrosine receptor kinase B ligand, neurotrophin-4, is not required for either epileptogenesis or tyrosine receptor kinase B activation in the kindling model. Neuroscience 141(1):515-20. [PubMed: 16650613] [MGI Ref ID J:110287]
Heppenstall PA; Lewin GR. 2001. BDNF but not NT-4 is required for normal flexion reflex plasticity and function. Proc Natl Acad Sci U S A 98(14):8107-12. [PubMed: 11438749] [MGI Ref ID J:126994]
Liu X; Jaenisch R. 2000. Severe peripheral sensory neuron loss and modest motor neuron reduction in mice with combined deficiency of brain-derived neurotrophic factor, neurotrophin 3 and neurotrophin 4/5. Dev Dyn 218(1):94-101. [PubMed: 10822262] [MGI Ref ID J:62072]
Lucas G; Hendolin P; Harkany T; Agerman K; Paratcha G; Holmgren C; Zilberter Y; Sairanen M; Minichiello L; Castren E; Ernfors P. 2003. Neurotrophin-4 mediated TrkB activation reinforces morphine-induced analgesia. Nat Neurosci 6(3):221-2. [PubMed: 12601381] [MGI Ref ID J:109298]
McIlwrath SL; Hu J; Anirudhan G; Shin JB; Lewin GR. 2005. The sensory mechanotransduction ion channel ASIC2 (acid sensitive ion channel 2) is regulated by neurotrophin availability. Neuroscience 131(2):499-511. [PubMed: 15708491] [MGI Ref ID J:105150]
Rice FL; Albers KM; Davis BM; Silos-Santiago I; Wilkinson GA; LeMaster AM; Ernfors P; Smeyne RJ; Aldskogius H; Phillips HS; Barbacid M; DeChiara TM; Yancopoulos GD; Dunne CE; Fundin BT. 1998. Differential dependency of unmyelinated and A delta epidermal and upper dermal innervation on neurotrophins, trk receptors, and p75LNGFR. Dev Biol 198(1):57-81. [PubMed: 9640332] [MGI Ref ID J:107715]
Roosen A; Schober A; Strelau J; Bottner M; Faulhaber J; Bendner G; McIlwrath SL; Seller H; Ehmke H; Lewin GR; Unsicker K. 2001. Lack of neurotrophin-4 causes selective structural and chemical deficits in sympathetic ganglia and their preganglionic innervation. J Neurosci 21(9):3073-84. [PubMed: 11312292] [MGI Ref ID J:109465]
Royo NC; Conte V; Saatman KE; Shimizu S; Belfield CM; Soltesz KM; Davis JE; Fujimoto ST; McIntosh TK. 2006. Hippocampal vulnerability following traumatic brain injury: a potential role for neurotrophin-4/5 in pyramidal cell neuroprotection. Eur J Neurosci 23(5):1089-102. [PubMed: 16553773] [MGI Ref ID J:107158]
Schober A; Wolf N; Huber K; Hertel R; Krieglstein K; Minichiello L; Kahane N; Widenfalk J; Kalcheim C; Olson L; Klein R; Lewin GR; Unsicker K. 1998. TrkB and neurotrophin-4 are important for development and maintenance of sympathetic preganglionic neurons innervating the adrenal medulla. J Neurosci 18(18):7272-84. [PubMed: 9736648] [MGI Ref ID J:120428]
Sedy J; Szeder V; Walro JM; Ren ZG; Nanka O; Tessarollo L; Sieber-Blum M; Grim M; Kucera J. 2004. Pacinian corpuscle development involves multiple Trk signaling pathways. Dev Dyn 231(3):551-63. [PubMed: 15376326] [MGI Ref ID J:93853]
Smith DJ; Leil TA; Liu X. 2003. Neurotrophin-4 is required for tolerance to morphine in the mouse. Neurosci Lett 340(2):103-6. [PubMed: 12668247] [MGI Ref ID J:107939]
Stucky CL; Shin JB; Lewin GR. 2002. Neurotrophin-4: a survival factor for adult sensory neurons. Curr Biol 12(16):1401-4. [PubMed: 12194821] [MGI Ref ID J:78592]
Xie CW; Sayah D; Chen QS; Wei WZ; Smith D; Liu X. 2000. Deficient long-term memory and long-lasting long-term potentiation in mice with a targeted deletion of neurotrophin-4 gene. Proc Natl Acad Sci U S A 97(14):8116-21. [PubMed: 10869436] [MGI Ref ID J:63270]
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
| Pricing for USA, Canada and Mexico shipping destinations |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00 Cryopreserved Embryos Fee $1600.00
| Pricing for International shipping destinations |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00 Cryopreserved Embryos Fee $2080.00
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
|
| Control | ||
|---|---|---|
| 002448 129S1/SvImJ | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. THE LABORATORY EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.
In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.
MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.
Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.