Strain Name:

C57BL/6J-Tg(WapIGFBP3)67Dlr/J

Stock Number:

002499

Availability:

Repository-Cryopreserved

Description

Strain Information

Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Mating System+/+ sibling x Hemizygote         (Female x Male)
Specieslaboratory mouse
GenerationN5+1 F3pN1
 
Donating Investigator Derek LeRoith,   Mount Sinai School of Medicine

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the WapIGFBP3 transgene are viable and fertile. The transgene is expressed at high levels during lactation and post-lactation. There were no obvious changes in mammary gland histomorphology during lactation, but at 5 to 10 days post-laction, there was a delay in involution and decreased apoptosis. This phenotype is identical to that of the C57BL/6J-TgN(WapIgf1)39Dlr strain mice.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Wap     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(WapIGFBP3)67Dlr/0

        involves: C57BL/6 * SJL
  • endocrine/exocrine gland phenotype
  • abnormal mammary gland morphology (MGI Ref ID J:33047)
    • alveoli are smaller than in wild-type mice
    • abnormal involution of the mammary gland (MGI Ref ID J:33047)
      • involution is delayed with fewer apoptotic cells than in wild-type mice
      • apoptosis during involution is decreased compared to in wild-type mice
      • remodeling during involution is retarded compared to in wild-type mice
    • mammary ductal hyperplasia (MGI Ref ID J:92578)
      • mice exhibit ductal hypertrophy accompanied by a thickened layer of collagen and extracellular matrix surrounding the flat epithelial cells
  • reproductive system phenotype
  • abnormal mammary gland morphology (MGI Ref ID J:33047)
    • alveoli are smaller than in wild-type mice
    • abnormal involution of the mammary gland (MGI Ref ID J:33047)
      • involution is delayed with fewer apoptotic cells than in wild-type mice
      • apoptosis during involution is decreased compared to in wild-type mice
      • remodeling during involution is retarded compared to in wild-type mice
    • mammary ductal hyperplasia (MGI Ref ID J:92578)
      • mice exhibit ductal hypertrophy accompanied by a thickened layer of collagen and extracellular matrix surrounding the flat epithelial cells
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

IGFBP3 related

Cancer Research
Growth Factors/Receptors/Cytokines

Endocrine Deficiency Research
Mammary Gland Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(WapIGFBP3)67Dlr
Allele Name transgene insertion 67, Derek LeRoith
Allele Type Transgenic (random, expressed)
Mutation Made By Derek LeRoith,   Mount Sinai School of Medicine
Strain of Origin(C57BL/6 x SJL)F2
Expressed Gene IGFBP3, insulin-like growth factor binding protein 3, human
Promoter Wap, whey acidic protein, rat
Molecular Note The transgene contains the mouse whey acidic protein promoter followed by the complete open reading frame of the human insulin-like growth factor binding protein 3 gene, including 116 bp of 5'UTR sequence and 523 bp of 3'UTR sequence. [MGI Ref ID J:92578]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(WapIGFBP3)67Dlr, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

LeRoith D; Neuenschwander S; Wood TL; Hennighausen L. 1995. Insulin-like growth factor-I and insulin-like growth factor binding protein-3 inhibit involution of the mammary gland following lactation: studies in transgenic mice. Prog Growth Factor Res 6(2-4):433-6. [PubMed: 8817687]  [MGI Ref ID J:92578]

Additional References

Neuenschwander S; Schwartz A; Wood TL; Roberts CT Jr; Henninghausen L; LeRoith D. 1996. Involution of the lactating mammary gland is inhibited by the IGF system in a transgenic mouse model. J Clin Invest 97(10):2225-32. [PubMed: 8636401]  [MGI Ref ID J:33047]

Tg(WapIGFBP3)67Dlr related

Neuenschwander S; Schwartz A; Wood TL; Roberts CT Jr; Henninghausen L; LeRoith D. 1996. Involution of the lactating mammary gland is inhibited by the IGF system in a transgenic mouse model. J Clin Invest 97(10):2225-32. [PubMed: 8636401]  [MGI Ref ID J:33047]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Mating System+/+ sibling x Hemizygote         (Female x Male)
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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