Strain Name:

C57BL/6-Il4tm1Nnt/J

Stock Number:

002518

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Mice homozygous for Il4tm1Nnthave reduced IgGl and IgE levels and reduced ability to produce Th2-derived cytokines.

Description

Strain Information

Type Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Specieslaboratory mouse
Generation?+N2F5 (10-DEC-13)
Generation Definitions

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Il4tm1Nnt targeted mutation are viable and fertile, but generate smaller liver granulomas that contained fewer eosinophils and no mast cells. Expression of the IL-4-dependent molecules IgE and IgG1 as well as B cell surface class II and CD23 is impaired. Production of Th2-derived cytokines is significantly reduced. T and B cell development is normal, and the lack of IL-4 appears not to affect CTL-mediated immunity.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Il4     (11 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Il4tm1Nnt/Il4tm1Nnt

        C57BL/6-Il4tm1Nnt/J
  • immune system phenotype
  • CNS inflammation
    • lethally irradiated mutant mice can be transplanted with bone marrow from wild-type mice and then have encephalomyelitis induced by injection of an inflammatory antigen   (MGI Ref ID J:125605)
    • these bone marrow chimera mice exhibit more severe inflammation with a 2-fold increase in the number of infiltrating lymphocytes, higher disease score, and earlier onset of disease   (MGI Ref ID J:125605)
    • the number of infiltrating macrophages and activated resident microglial cells are also increased in these bone marrow chimeras as a result of encephalomyelitis induction   (MGI Ref ID J:125605)
  • abnormal T-helper 2 physiology
    • lymphocytes isolated from liver granulomas resulting from schistosome infection lack a strong Th2 response   (MGI Ref ID J:37196)
    • the lack of Th2 response is reflected in no secretion of IL-4, IL-5, and a 50% reduction in IL-10 by lymphocytes isolated from liver granulomas   (MGI Ref ID J:37196)
    • liver granuloma lymphocytes make small amounts of IFN-gamma compared to wild-type lymphocytes that make no IFN-gamma   (MGI Ref ID J:37196)
  • abnormal microglial cell morphology
    • microglial cells fail to express the cell marker Ym1 (Chi3l3)   (MGI Ref ID J:125605)
  • decreased IgE level
    • lymphocytes from the spleen or from liver granulomas fail to secrete IgE in response to Schistosoma mansoni infection   (MGI Ref ID J:37196)
  • decreased IgG1 level
    • serum IgG1 levels are dramatically decreased in response to schistosome infection compared to the wild-type response   (MGI Ref ID J:37196)
    • isolated lymphocytes from the spleen or from liver granulomas produce very little IgG1 in culture   (MGI Ref ID J:37196)
  • decreased IgM level
    • very little IgM is produced by lymphocytes that are isolated from liver granulomas   (MGI Ref ID J:37196)
  • decreased interleukin-10 secretion
    • activated splenic lymphocytes fail to secrete IL-10   (MGI Ref ID J:37196)
    • IL-10 secretion by lymphocytes isolated from liver granulomas is also severely impaired   (MGI Ref ID J:37196)
    • splenocytes from Helicobacter pylori infected mice produce 4-fold less IL-10 when cultured in the presence of H. pylori antigen   (MGI Ref ID J:120556)
  • decreased interleukin-4 secretion
    • lymphocytes make no IL-4   (MGI Ref ID J:37196)
  • decreased interleukin-5 secretion
    • secretion of IL-5 is almost absent by unactivated lymphocytes isolated from liver granulomas   (MGI Ref ID J:37196)
    • activated lymphocytes from liver granulomas produced 50% IL-5 compared to controls   (MGI Ref ID J:37196)
    • secretion of IL-5 by activated splenic lymphocytes is also severely impaired   (MGI Ref ID J:37196)
  • granulomatous inflammation
    • mice generate 32% smaller liver granulomas than wild-type mice in response to schistosome ova 8 weeks after infection   (MGI Ref ID J:37196)
    • the granulomas contain few eosinophils and no mast cells compared to their wild-type counterparts   (MGI Ref ID J:37196)
    • granulomas contain2-fold more lymphocytes and 42% more macrophages than in wild-type mice   (MGI Ref ID J:37196)
    • the number of B cells in the liver granulomas increases more than 2-fold while the number of CD4 T cells decreases by almost the same amount   (MGI Ref ID J:37196)
    • B cells in granulomas have lower surface expression of Class II and IgE receptor   (MGI Ref ID J:37196)
  • increased IgA level
    • there is enhanced IgA production by lymphocytes isolated from either the spleen or liver granulomas after schistosome infection   (MGI Ref ID J:37196)
  • increased interferon-gamma secretion
    • lymphocytes isolated from liver granulomas produce dectectable amounts of IFN-gamma compared to wild-type lymphocytes that produce no IFN-gamma   (MGI Ref ID J:37196)
    • splenocytes from Helicobacter pylori-infected mice produce log fold higher amounts of IFN-gamma than splenocytes from wild-type controls when cultured in the presence of H. pylori antigen   (MGI Ref ID J:120556)
  • stomach inflammation
    • 5 weeks after Helicobacter pylori infection, mice have a significantly increased gastritis inflammation score compared to infected wild-type mice   (MGI Ref ID J:120556)
  • digestive/alimentary phenotype
  • stomach inflammation
    • 5 weeks after Helicobacter pylori infection, mice have a significantly increased gastritis inflammation score compared to infected wild-type mice   (MGI Ref ID J:120556)
  • hematopoietic system phenotype
  • abnormal T-helper 2 physiology
    • lymphocytes isolated from liver granulomas resulting from schistosome infection lack a strong Th2 response   (MGI Ref ID J:37196)
    • the lack of Th2 response is reflected in no secretion of IL-4, IL-5, and a 50% reduction in IL-10 by lymphocytes isolated from liver granulomas   (MGI Ref ID J:37196)
    • liver granuloma lymphocytes make small amounts of IFN-gamma compared to wild-type lymphocytes that make no IFN-gamma   (MGI Ref ID J:37196)
  • abnormal microglial cell morphology
    • microglial cells fail to express the cell marker Ym1 (Chi3l3)   (MGI Ref ID J:125605)
  • decreased IgE level
    • lymphocytes from the spleen or from liver granulomas fail to secrete IgE in response to Schistosoma mansoni infection   (MGI Ref ID J:37196)
  • decreased IgG1 level
    • serum IgG1 levels are dramatically decreased in response to schistosome infection compared to the wild-type response   (MGI Ref ID J:37196)
    • isolated lymphocytes from the spleen or from liver granulomas produce very little IgG1 in culture   (MGI Ref ID J:37196)
  • decreased IgM level
    • very little IgM is produced by lymphocytes that are isolated from liver granulomas   (MGI Ref ID J:37196)
  • increased IgA level
    • there is enhanced IgA production by lymphocytes isolated from either the spleen or liver granulomas after schistosome infection   (MGI Ref ID J:37196)
  • nervous system phenotype
  • CNS inflammation
    • lethally irradiated mutant mice can be transplanted with bone marrow from wild-type mice and then have encephalomyelitis induced by injection of an inflammatory antigen   (MGI Ref ID J:125605)
    • these bone marrow chimera mice exhibit more severe inflammation with a 2-fold increase in the number of infiltrating lymphocytes, higher disease score, and earlier onset of disease   (MGI Ref ID J:125605)
    • the number of infiltrating macrophages and activated resident microglial cells are also increased in these bone marrow chimeras as a result of encephalomyelitis induction   (MGI Ref ID J:125605)
  • abnormal microglial cell morphology
    • microglial cells fail to express the cell marker Ym1 (Chi3l3)   (MGI Ref ID J:125605)

Il4tm1Nnt/Il4tm1Nnt

        involves: C57BL/6
  • immune system phenotype
  • abnormal interleukin-4 secretion
    • lymphocytes fail to produce IL-4   (MGI Ref ID J:91403)
  • decreased susceptibility to autoimmune disorder
    • some mice have a less severe degree of airway hyperresponsiveness after sensitization and challenge with OVA peptide   (MGI Ref ID J:91403)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Il4tm1Nnt related

Cancer Research
Growth Factors/Receptors/Cytokines

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Il4tm1Nnt
Allele Name targeted mutation 1, Nancy Noben-Trauth
Allele Type Targeted (Null/Knockout)
Common Name(s) IL-4-; il4-;
Mutation Made By Nancy Noben-Trauth,  
Strain of OriginC57BL/6J
ES Cell Line NameBL/6-III
ES Cell Line StrainC57BL/6J
Gene Symbol and Name Il4, interleukin 4
Chromosome 11
Gene Common Name(s) BCGF-1; BCGF1; BSF-1; BSF1; IL-4; Il-4; Il4e12;
Molecular Note A neomycin resistance cassette was inserted into exon 3 of the gene. [MGI Ref ID J:36386]

Genotyping

Genotyping Information

Genotyping Protocols

Il4tm1Nnt, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Metwali A; Elliott D; Blum AM; Li J; Sandor M; Lynch R; Noben-Trauth N; Weinstock JV. 1996. The granulomatous response in murine Schistosomiasis mansoni does not switch to Th1 in IL-4-deficient C57BL/6 mice. J Immunol 157(10):4546-53. [PubMed: 8906833]  [MGI Ref ID J:37196]

Additional References

Bitsaktsis C; Huntington J; Winslow G. 2004. Production of IFN-gamma by CD4 T cells is essential for resolving ehrlichia infection. J Immunol 172(11):6894-901. [PubMed: 15153508]  [MGI Ref ID J:90518]

Dohi T; Fujihashi K; Kiyono H; Elson CO; McGhee JR. 2000. Mice deficient in Th1- and Th2-type cytokines develop distinct forms of hapten-induced colitis. Gastroenterology 119(3):724-33. [PubMed: 10982767]  [MGI Ref ID J:64230]

Hadeiba H; Corry DB; Locksley RM. 2000. Baseline airway hyperreactivity in A/J mice is not mediated by cells of the adaptive immune system. J Immunol 164(9):4933-40. [PubMed: 10779804]  [MGI Ref ID J:61706]

Hall LR; Berger RB; Diaconu E; Pearlman E. 2002. Onchocerca volvulus keratitis (river blindness) is exacerbated in BALB/c IL-4 gene knockout mice. Cell Immunol 216(1-2):1-5. [PubMed: 12381344]  [MGI Ref ID J:80510]

Herrick CA; Xu L; McKenzie AN; Tigelaar RE; Bottomly K. 2003. IL-13 is necessary, not simply sufficient, for epicutaneously induced Th2 responses to soluble protein antigen. J Immunol 170(5):2488-95. [PubMed: 12594274]  [MGI Ref ID J:81985]

Spencer L; Shultz L; Rajan TV. 2001. Interleukin-4 receptor-stat6 signaling in murine infections with a tissue-dwelling nematode parasite. Infect Immun 69(12):7743-52. [PubMed: 11705956]  [MGI Ref ID J:73137]

Il4tm1Nnt related

Akbari O; Stock P; Meyer E; Kronenberg M; Sidobre S; Nakayama T; Taniguchi M; Grusby MJ; DeKruyff RH; Umetsu DT. 2003. Essential role of NKT cells producing IL-4 and IL-13 in the development of allergen-induced airway hyperreactivity. Nat Med 9(5):582-8. [PubMed: 12669034]  [MGI Ref ID J:84720]

Allen HL; Deepe GS Jr. 2005. Apoptosis modulates protective immunity to the pathogenic fungus Histoplasma capsulatum. J Clin Invest 115(10):2875-85. [PubMed: 16151533]  [MGI Ref ID J:101533]

Bancroft AJ; Artis D; Donaldson DD; Sypek JP; Grencis RK. 2000. Gastrointestinal nematode expulsion in IL-4 knockout mice is IL-13 dependent. Eur J Immunol 30(7):2083-91. [PubMed: 10940898]  [MGI Ref ID J:63513]

Bry L; Brigl M; Brenner MB. 2006. CD4+-T-cell effector functions and costimulatory requirements essential for surviving mucosal infection with Citrobacter rodentium. Infect Immun 74(1):673-81. [PubMed: 16369024]  [MGI Ref ID J:104251]

Buxbaum LU; Scott P. 2005. Interleukin 10- and Fcgamma receptor-deficient mice resolve Leishmania mexicana lesions. Infect Immun 73(4):2101-8. [PubMed: 15784551]  [MGI Ref ID J:97206]

Campos RA; Szczepanik M; Itakura A; Akahira-Azuma M; Sidobre S; Kronenberg M; Askenase PW. 2003. Cutaneous immunization rapidly activates liver invariant Valpha14 NKT cells stimulating B-1 B cells to initiate T cell recruitment for elicitation of contact sensitivity. J Exp Med 198(12):1785-96. [PubMed: 14676294]  [MGI Ref ID J:132827]

Cardoso CR; Provinciatto PR; Godoi DF; Ferreira BR; Teixeira G; Rossi MA; Cunha FQ; Silva JS. 2009. IL-4 regulates susceptibility to intestinal inflammation in murine food allergy. Am J Physiol Gastrointest Liver Physiol 296(3):G593-600. [PubMed: 19136382]  [MGI Ref ID J:146543]

Chensue SW; Warmington K; Ruth JH; Lukacs N; Kunkel SL. 1997. Mycobacterial and schistosomal antigen-elicited granuloma formation in IFN-gamma and IL-4 knockout mice: analysis of local and regional cytokine and chemokine networks. J Immunol 159(7):3565-73. [PubMed: 9317156]  [MGI Ref ID J:43085]

Chiaramonte MG; Donaldson DD; Cheever AW; Wynn TA. 1999. An IL-13 inhibitor blocks the development of hepatic fibrosis during a T-helper type 2-dominated inflammatory response. J Clin Invest 104(6):777-85. [PubMed: 10491413]  [MGI Ref ID J:115354]

Cole N; Hume E; Khan S; Krockenberger M; Thakur A; Husband AJ; Willcox MD. 2005. Interleukin-4 is not critical to pathogenesis in a mouse model of Pseudomonas aeruginosa corneal infection. Curr Eye Res 30(7):535-42. [PubMed: 16020287]  [MGI Ref ID J:123047]

Denkinger CM; Denkinger MD; Forsthuber TG. 2007. Pertussis toxin-induced cytokine differentiation and clonal expansion of T cells is mediated predominantly via costimulation. Cell Immunol 246(1):46-54. [PubMed: 17601518]  [MGI Ref ID J:123551]

Dohi T; Fujihashi K; Kiyono H; Elson CO; McGhee JR. 2000. Mice deficient in Th1- and Th2-type cytokines develop distinct forms of hapten-induced colitis. Gastroenterology 119(3):724-33. [PubMed: 10982767]  [MGI Ref ID J:64230]

Fischer R; Tome D; McGhee JR; Boyaka PN. 2007. Th1 and Th2 cells are required for both eosinophil- and neutrophil-associated airway inflammatory responses in mice. Biochem Biophys Res Commun 357(1):44-9. [PubMed: 17412309]  [MGI Ref ID J:121793]

Gao M; Labuda T; Xia Y; Gallagher E; Fang D; Liu YC; Karin M. 2004. Jun turnover is controlled through JNK-dependent phosphorylation of the E3 ligase Itch. Science 306(5694):271-5. [PubMed: 15358865]  [MGI Ref ID J:93082]

Ghoreschi K; Bruck J; Kellerer C; Deng C; Peng H; Rothfuss O; Hussain RZ; Gocke AR; Respa A; Glocova I; Valtcheva N; Alexander E; Feil S; Feil R; Schulze-Osthoff K; Rupec RA; Lovett-Racke AE; Dringen R; Racke MK; Rocken M. 2011. Fumarates improve psoriasis and multiple sclerosis by inducing type II dendritic cells. J Exp Med 208(11):2291-303. [PubMed: 21987655]  [MGI Ref ID J:178767]

Gregory GD; Raju SS; Winandy S; Brown MA. 2006. Mast cell IL-4 expression is regulated by Ikaros and influences encephalitogenic Th1 responses in EAE. J Clin Invest 116(5):1327-36. [PubMed: 16628252]  [MGI Ref ID J:108943]

Halim TY; Steer CA; Matha L; Gold MJ; Martinez-Gonzalez I; McNagny KM; McKenzie AN; Takei F. 2014. Group 2 innate lymphoid cells are critical for the initiation of adaptive T helper 2 cell-mediated allergic lung inflammation. Immunity 40(3):425-35. [PubMed: 24613091]  [MGI Ref ID J:210240]

Hepworth MR; Hardman MJ; Grencis RK. 2010. The role of sex hormones in the development of Th2 immunity in a gender-biased model of Trichuris muris infection. Eur J Immunol 40(2):406-16. [PubMed: 19950176]  [MGI Ref ID J:157675]

Hernandez Y; Arora S; Erb-Downward JR; McDonald RA; Toews GB; Huffnagle GB. 2005. Distinct roles for IL-4 and IL-10 in regulating T2 immunity during allergic bronchopulmonary mycosis. J Immunol 174(2):1027-36. [PubMed: 15634927]  [MGI Ref ID J:95831]

Hwang ES; White IA; Ho IC. 2002. An IL-4-independent and CD25-mediated function of c-maf in promoting the production of Th2 cytokines. Proc Natl Acad Sci U S A 99(20):13026-30. [PubMed: 12271139]  [MGI Ref ID J:134605]

Iijima H; Neurath MF; Nagaishi T; Glickman JN; Nieuwenhuis EE; Nakajima A; Chen D; Fuss IJ; Utku N; Lewicki DN; Becker C; Gallagher TM; Holmes KV; Blumberg RS. 2004. Specific regulation of T helper cell 1-mediated murine colitis by CEACAM1. J Exp Med 199(4):471-82. [PubMed: 14970176]  [MGI Ref ID J:90476]

Jenkins SJ; Ruckerl D; Thomas GD; Hewitson JP; Duncan S; Brombacher F; Maizels RM; Hume DA; Allen JE. 2013. IL-4 directly signals tissue-resident macrophages to proliferate beyond homeostatic levels controlled by CSF-1. J Exp Med 210(11):2477-91. [PubMed: 24101381]  [MGI Ref ID J:204583]

Jin H; Gong D; Adeegbe D; Bayer AL; Rolle C; Yu A; Malek TR. 2006. Quantitative assessment concerning the contribution of IL-2R{beta} for superantigen-mediated T cell responses in vivo. Int Immunol 18(4):565-72. [PubMed: 16540525]  [MGI Ref ID J:107065]

Jones TG; Finkelman FD; Austen KF; Gurish MF. 2010. T regulatory cells control antigen-induced recruitment of mast cell progenitors to the lungs of C57BL/6 mice. J Immunol 185(3):1804-11. [PubMed: 20601599]  [MGI Ref ID J:162451]

Keane MP; Gomperts BN; Weigt S; Xue YY; Burdick MD; Nakamura H; Zisman DA; Ardehali A; Saggar R; Lynch JP 3rd; Hogaboam C; Kunkel SL; Lukacs NW; Ross DJ; Grusby MJ; Strieter RM; Belperio JA. 2007. IL-13 is pivotal in the fibro-obliterative process of bronchiolitis obliterans syndrome. J Immunol 178(1):511-9. [PubMed: 17182591]  [MGI Ref ID J:141922]

Kleinschek MA; Owyang AM; Joyce-Shaikh B; Langrish CL; Chen Y; Gorman DM; Blumenschein WM; McClanahan T; Brombacher F; Hurst SD; Kastelein RA; Cua DJ. 2007. IL-25 regulates Th17 function in autoimmune inflammation. J Exp Med 204(1):161-70. [PubMed: 17200411]  [MGI Ref ID J:125296]

Le Saux CJ; Teeters K; Miyasato SK; Hoffmann PR; Bollt O; Douet V; Shohet RV; Broide DH; Tam EK. 2008. Down-regulation of caveolin-1, an inhibitor of transforming growth factor-beta signaling, in acute allergen-induced airway remodeling. J Biol Chem 283(9):5760-8. [PubMed: 18056268]  [MGI Ref ID J:132297]

Leadbetter EA; Brigl M; Illarionov P; Cohen N; Luteran MC; Pillai S; Besra GS; Brenner MB. 2008. NK T cells provide lipid antigen-specific cognate help for B cells. Proc Natl Acad Sci U S A 105(24):8339-44. [PubMed: 18550809]  [MGI Ref ID J:137193]

Li X; Wang Y; Urso D; O'Rourke J; Cone RE. 2004. Thymocytes induced by antigen injection into the anterior chamber activate splenic CD8+ suppressor cells and enhance the antigen-induced production of immunoglobulin G1 antibodies. Immunology 113(1):44-56. [PubMed: 15312135]  [MGI Ref ID J:92936]

Liu Z; Liu Q; Hamed H; Anthony RM; Foster A; Finkelman FD; Urban JF Jr; Gause WC. 2005. IL-2 and autocrine IL-4 drive the in vivo development of antigen-specific Th2 T cells elicited by nematode parasites. J Immunol 174(4):2242-9. [PubMed: 15699158]  [MGI Ref ID J:96546]

Marques VP; Goncalves GM; Feitoza CQ; Cenedeze MA; Fernandes Bertocchi AP; Damiao MJ; Pinheiro HS; Antunes Teixeira VP; dos Reis MA; Pacheco-Silva A; Saraiva Camara NO. 2006. Influence of TH1/TH2 switched immune response on renal ischemia-reperfusion injury. Nephron Exp Nephrol 104(1):e48-56. [PubMed: 16741373]  [MGI Ref ID J:127477]

McDermott JR; Humphreys NE; Forman SP; Donaldson DD; Grencis RK. 2005. Intraepithelial NK cell-derived IL-13 induces intestinal pathology associated with nematode infection. J Immunol 175(5):3207-13. [PubMed: 16116211]  [MGI Ref ID J:113332]

Noben-Trauth N; Kohler G; Burki K; Ledermann B. 1996. Efficient targeting of the IL-4 gene in a BALB/c embryonic stem cell line. Transgenic Res 5(6):487-91. [PubMed: 8840532]  [MGI Ref ID J:36386]

Ong CJ; Ip S; Teh SJ; Wong C; Jirik FR; Grusby MJ; Teh HS. 1999. A role for T helper 2 cells in mediating skin fibrosis in tight-skin mice. Cell Immunol 196(1):60-8. [PubMed: 10486156]  [MGI Ref ID J:57957]

Panthel K; Faller G; Haas R. 2003. Colonization of C57BL/6J and BALB/c wild-type and knockout mice with Helicobacter pylori: effect of vaccination and implications for innate and acquired immunity. Infect Immun 71(2):794-800. [PubMed: 12540559]  [MGI Ref ID J:81703]

Peng S; Wu H; Mo YY; Watabe K; Pauza ME. 2009. c-Maf increases apoptosis in peripheral CD8 cells by transactivating Caspase 6. Immunology 127(2):267-78. [PubMed: 19476513]  [MGI Ref ID J:155649]

Ponomarev ED; Maresz K; Tan Y; Dittel BN. 2007. CNS-derived interleukin-4 is essential for the regulation of autoimmune inflammation and induces a state of alternative activation in microglial cells. J Neurosci 27(40):10714-21. [PubMed: 17913905]  [MGI Ref ID J:125605]

Porter BO; Malek TR. 1999. IL-2Rbeta/IL-7Ralpha doubly deficient mice recapitulate the thymic and intraepithelial lymphocyte (IEL) developmental defects of gammac-/- mice: roles for both IL-2 and IL-15 in CD8alphaalpha IEL development. J Immunol 163(11):5906-12. [PubMed: 10570276]  [MGI Ref ID J:58655]

Potter MR; Noben-Trauth N; Weis JH; Teuscher C; Weis JJ. 2000. Interleukin-4 (IL-4) and IL-13 signaling pathways Do not regulate borrelia burgdorferi-induced arthritis in mice: IgG1 is not required for host control of tissue spirochetes Infect Immun 68(10):5603-9. [PubMed: 10992460]  [MGI Ref ID J:64838]

Qin M; Kang J; Smith CB. 2002. Increased rates of cerebral glucose metabolism in a mouse model of fragile X mental retardation. Proc Natl Acad Sci U S A 99(24):15758-63. [PubMed: 12427968]  [MGI Ref ID J:80518]

Rankin AL; Mumm JB; Murphy E; Turner S; Yu N; McClanahan TK; Bourne PA; Pierce RH; Kastelein R; Pflanz S. 2010. IL-33 induces IL-13-dependent cutaneous fibrosis. J Immunol 184(3):1526-35. [PubMed: 20042577]  [MGI Ref ID J:159528]

Rengarajan J; Tang B; Glimcher LH. 2002. NFATc2 and NFATc3 regulate T(H)2 differentiation and modulate TCR-responsiveness of naive T(H)cells. Nat Immunol 3(1):48-54. [PubMed: 11740499]  [MGI Ref ID J:109185]

Roelofs-Haarhuis K; Wu X; Gleichmann E. 2004. Oral tolerance to nickel requires CD4+ invariant NKT cells for the infectious spread of tolerance and the induction of specific regulatory T cells. J Immunol 173(2):1043-50. [PubMed: 15240692]  [MGI Ref ID J:91933]

Saleem S; Dai Z; Coelho SN; Konieczny BT; Assmann KJ; Baddoura FK; Lakkis FG. 1998. IL-4 is an endogenous inhibitor of neutrophil influx and subsequent pathology in acute antibody-mediated inflammation. J Immunol 160(2):979-84. [PubMed: 9551937]  [MGI Ref ID J:45149]

Smythies LE; Waites KB; Lindsey JR; Harris PR; Ghiara P; Smith PD. 2000. Helicobacter pylori-induced mucosal inflammation is Th1 mediated and exacerbated in IL-4, but not IFN-gamma, gene-deficient mice. J Immunol 165(2):1022-9. [PubMed: 10878379]  [MGI Ref ID J:120556]

Spencer L; Shultz L; Rajan TV. 2001. Interleukin-4 receptor-stat6 signaling in murine infections with a tissue-dwelling nematode parasite. Infect Immun 69(12):7743-52. [PubMed: 11705956]  [MGI Ref ID J:73137]

Villacres MC; Bergmann CC. 1999. Enhanced cytotoxic T cell activity in IL-4-deficient mice. J Immunol 162(5):2663-70. [PubMed: 10072509]  [MGI Ref ID J:110849]

Walter DM; McIntire JJ; Berry G; McKenzie AN; Donaldson DD; DeKruyff RH; Umetsu DT. 2001. Critical role for IL-13 in the development of allergen-induced airway hyperreactivity. J Immunol 167(8):4668-75. [PubMed: 11591797]  [MGI Ref ID J:91403]

Woolard MD; Hodge LM; Jones HP; Schoeb TR; Simecka JW. 2004. The upper and lower respiratory tracts differ in their requirement of IFN-gamma and IL-4 in controlling respiratory mycoplasma infection and disease. J Immunol 172(11):6875-83. [PubMed: 15153506]  [MGI Ref ID J:90522]

Zhao A; Morimoto M; Dawson H; Elfrey JE; Madden KB; Gause WC; Min B; Finkelman FD; Urban JF Jr; Shea-Donohue T. 2005. Immune regulation of protease-activated receptor-1 expression in murine small intestine during Nippostrongylus brasiliensis infection. J Immunol 175(4):2563-9. [PubMed: 16081830]  [MGI Ref ID J:107492]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHomozygous for Il4tm1Nnt  
Price per Pair (US dollars $)Pair Genotype
$399.80Homozygous for Il4tm1Nnt x Homozygous for Il4tm1Nnt  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHomozygous for Il4tm1Nnt  
Price per Pair (US dollars $)Pair Genotype
$519.80Homozygous for Il4tm1Nnt x Homozygous for Il4tm1Nnt  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

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Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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