Strain Name:

C.129S4-Myf5tm1Jae/J

Stock Number:

002522

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain BALB/c
Donor Strain 129S4 via J1 ES cell line
 
Donating InvestigatorDr. Rudolf Jaenisch,   Whitehead Institute (MIT)

Description
Mice homozygous for a targeted mutation in the Myf5 gene die perinatally from respiratory failure due to improper development of the rib cage. Ribs are truncated and the sternum is shortened, with no sternebral segmentation and no connection to the ribs. Mutant mice show no obvious alterations in the vertebral column in comparison to wild type controls, and there are no skeletal muscle abnormalities apparent at birth.

Control Information

  Control
   Wild-type from the colony
   000651 BALB/cJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Myf5
007893   B6.129S4-Myf5tm3(cre)Sor/J
010529   B6;129-Myf5tm1(cre)Mrc/J
023342   STOCK Myf5tm1(cre/Esr1*)Trdo/J
018626   STOCK Myf5tm1Pas/J
View Strains carrying other alleles of Myf5     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Myf5tm1Jae/Myf5tm1Jae

        involves: 129S4/SvJae
  • skeleton phenotype
  • abnormal rib-sternum attachment
    • no contacts with the sternum are made because the ribs are short   (MGI Ref ID J:24360)
  • abnormal sternum ossification
    • sternum is completely ossified   (MGI Ref ID J:24360)
  • rib bifurcation   (MGI Ref ID J:42453)
  • short ribs   (MGI Ref ID J:24360)
    • mutants lack the distal parts of the ribs; ribs are shorter than in Myf6 homozygotes   (MGI Ref ID J:31636)
  • muscle phenotype
  • absent myotome
    • no myotomes are detected at E10.5   (MGI Ref ID J:42453)

Myf5tm1Jae/Myf5tm1Jae

        either: 129-Myf5tm1Jae or (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
  • mortality/aging
  • complete neonatal lethality
    • die immediately after birth   (MGI Ref ID J:3013)
  • respiratory system phenotype
  • respiratory failure
    • unable to breathe   (MGI Ref ID J:3013)
  • skeleton phenotype
  • abnormal rib-sternum attachment
    • ribs do not attach to the sternum due to their truncation   (MGI Ref ID J:3013)
  • abnormal sternum ossification
    • the sternum is completely ossified without sternebral segmentation   (MGI Ref ID J:3013)
  • short ribs
    • absence of the major distal part of the ribs; the cranial rib stumps on both sides of the vertebral column end in dilated enlarged structures   (MGI Ref ID J:3013)
  • short sternum   (MGI Ref ID J:3013)
  • embryogenesis phenotype
  • abnormal somite development
    • appearance of myotomal cells in early somites is delayed by several days   (MGI Ref ID J:3013)
  • muscle phenotype
  • abnormal muscle morphology
    • organization of muscles in the anterior thoracic wall is disorganized, probably due to lack of ribs   (MGI Ref ID J:3013)
    • abnormal myotome development
      • muscle-specific marker analysis indicates a delay in the development of the myotome   (MGI Ref ID J:3013)

Myf5tm1Jae/Myf5tm1Jae

        involves: 129S4/SvJae * C57BL/6J
  • skeleton phenotype
  • fusion of vertebral arches
    • animals show extensive vertebral arch fusions compared to wild-type   (MGI Ref ID J:65301)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Myf5tm1Jae related

Developmental Biology Research
Mesodermal Defects
      Myogenesis Defects
Skeletal Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Myf5tm1Jae
Allele Name targeted mutation 1, Rudolf Jaenisch
Allele Type Targeted (knock-out)
Common Name(s) Myf-5 (-); Myf-5m1; Myf5Jae;
Mutation Made ByDr. Rudolf Jaenisch,   Whitehead Institute (MIT)
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Myf5, myogenic factor 5
Chromosome 10
Gene Common Name(s) B130010J22Rik; Myf-5; RIKEN cDNA B130010J22 gene; bHLHc2;
General Note Mice homozygous for Myf6 mutations lack or show reduced Myf5 expression and exhibit phenotypes seen in Myf5tm1Jae homozygotes. Studies have shown that Myf6 may regulate Myf5 expression by a cis-acting mechanism and that the skeletal abnormalities seen in Myf6 homozygotes may result from a decrease or lack of Myf5 expression (J:24360, J:31636).
Molecular Note A PGK-neomycin cassette was inserted into exon 1 of the gene, disrupting the reading frame. [MGI Ref ID J:3013]

Genotyping

Genotyping Information

Genotyping Protocols

Myf-5tm1Jae, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Braun T; Rudnicki MA; Arnold HH; Jaenisch R. 1992. Targeted inactivation of the muscle regulatory gene Myf-5 results in abnormal rib development and perinatal death. Cell 71(3):369-82. [PubMed: 1423602]  [MGI Ref ID J:3013]

Additional References

Rudnicki MA; Schnegelsberg PN; Stead RH; Braun T; Arnold HH; Jaenisch R. 1993. MyoD or Myf-5 is required for the formation of skeletal muscle. Cell 75(7):1351-9. [PubMed: 8269513]  [MGI Ref ID J:16387]

Myf5tm1Jae related

Angka HE; Geddes AJ; Kablar B. 2008. Differential survival response of neurons to exogenous GDNF depends on the presence of skeletal muscle. Dev Dyn 237(11):3169-78. [PubMed: 18816441]  [MGI Ref ID J:143363]

Angka HE; Kablar B. 2007. Differential responses to the application of exogenous NT-3 are observed for subpopulations of motor and sensory neurons depending on the presence of skeletal muscle. Dev Dyn 236(5):1193-202. [PubMed: 17436272]  [MGI Ref ID J:121117]

Baguma-Nibasheka M; Angka HE; Inanlou MR; Kablar B. 2007. Microarray analysis of Myf5-/-:MyoD-/- hypoplastic mouse lungs reveals a profile of genes involved in pneumocyte differentiation. Histol Histopathol 22(5):483-95. [PubMed: 17330803]  [MGI Ref ID J:121955]

Bidwell MC; Eitzman BA; Walmer DK; McLachlan JA; Gray KD. 1995. Analysis of messenger ribonucleic acid and protein for the ligands and receptors of the platelet-derived growth factor signaling pathway in the placenta, extraembryonic membranes, and uterus during the latter half of murine gestation. Endocrinology 136(11):5189-201. [PubMed: 7588258]  [MGI Ref ID J:29762]

Braun T; Arnold HH. 1995. Inactivation of Myf-6 and Myf-5 genes in mice leads to alterations in skeletal muscle development. EMBO J 14(6):1176-86. [PubMed: 7720708]  [MGI Ref ID J:24360]

Braun T; Bober E; Rudnicki MA; Jaenisch R; Arnold HH. 1994. MyoD expression marks the onset of skeletal myogenesis in Myf-5 mutant mice. Development 120(11):3083-92. [PubMed: 7720554]  [MGI Ref ID J:21616]

Chen JC; Goldhamer DJ. 2004. The core enhancer is essential for proper timing of MyoD activation in limb buds and branchial arches. Dev Biol 265(2):502-12. [PubMed: 14732408]  [MGI Ref ID J:87370]

Chen JC; Ramachandran R; Goldhamer DJ. 2002. Essential and redundant functions of the MyoD distal regulatory region revealed by targeted mutagenesis. Dev Biol 245(1):213-23. [PubMed: 11969267]  [MGI Ref ID J:76260]

Floss T; Arnold HH; Braun T. 1996. Myf-5(m1)/Myf-6(m1) compound heterozygous mouse mutants down-regulate Myf-5 expression and exert rib defects: evidence for long-range cis effects on Myf-5 transcription. Dev Biol 174(1):140-7. [PubMed: 8626014]  [MGI Ref ID J:31636]

Garry DJ; Yang Q; Bassel-Duby R; Williams RS. 1997. Persistent expression of MNF identifies myogenic stem cells in postnatal muscles. Dev Biol 188(2):280-94. [PubMed: 9268575]  [MGI Ref ID J:42845]

Gomez C; David V; Peet NM; Vico L; Chenu C; Malaval L; Skerry TM. 2007. Absence of mechanical loading in utero influences bone mass and architecture but not innervation in Myod-Myf5-deficient mice. J Anat 210(3):259-71. [PubMed: 17331176]  [MGI Ref ID J:124478]

Grass S; Arnold HH; Braun T. 1996. Alterations in somite patterning of Myf-5-deficient mice: a possible role for FGF-4 and FGF-6. Development 122(1):141-50. [PubMed: 8565825]  [MGI Ref ID J:30824]

Inanlou MR; Kablar B. 2005. Abnormal development of the intercostal muscles and the rib cage in Myf5-/- embryos leads to pulmonary hypoplasia. Dev Dyn 232(1):43-54. [PubMed: 15580568]  [MGI Ref ID J:95138]

Inanlou MR; Kablar B. 2005. Contractile activity of skeletal musculature involved in breathing is essential for normal lung cell differentiation, as revealed in Myf5-/-:MyoD-/- embryos. Dev Dyn 233(3):772-782. [PubMed: 15844178]  [MGI Ref ID J:98811]

Kablar B. 2003. Determination of retinal cell fates is affected in the absence of extraocular striated muscles. Dev Dyn 226(3):478-90. [PubMed: 12619134]  [MGI Ref ID J:82209]

Kablar B; Asakura A; Krastel K; Ying C; May LL; Goldhamer DJ ; Rudnicki MA. 1998. MyoD and Myf-5 define the specification of musculature of distinct embryonic origin. Biochem Cell Biol 76(6):1079-91. [PubMed: 10392718]  [MGI Ref ID J:55565]

Kablar B; Belliveau AC. 2005. Presence of neurotrophic factors in skeletal muscle correlates with survival of spinal cord motor neurons. Dev Dyn 234(3):659-69. [PubMed: 16193506]  [MGI Ref ID J:102289]

Kablar B; Krastel K; Tajbakhsh S; Rudnicki MA. 2003. Myf5 and MyoD activation define independent myogenic compartments during embryonic development. Dev Biol 258(2):307-18. [PubMed: 12798290]  [MGI Ref ID J:83932]

Kablar B; Krastel K; Ying C; Asakura A; Tapscott SJ; Rudnicki MA. 1997. MyoD and Myf-5 differentially regulate the development of limb versus trunk skeletal muscle. Development 124(23):4729-38. [PubMed: 9428409]  [MGI Ref ID J:45349]

Kablar B; Rudnicki MA. 1999. Development in the absence of skeletal muscle results in the sequential ablation of motor neurons from the spinal cord to the brain. Dev Biol 208(1):93-109. [PubMed: 10075844]  [MGI Ref ID J:54064]

Kablar B; Rudnicki MA. 2002. Information provided by the skeletal muscle and associated neurons is necessary for proper brain development. Int J Dev Neurosci 20(7):573-84. [PubMed: 12485625]  [MGI Ref ID J:100037]

Kahn J; Shwartz Y; Blitz E; Krief S; Sharir A; Breitel DA; Rattenbach R; Relaix F; Maire P; Rountree RB; Kingsley DM; Zelzer E. 2009. Muscle contraction is necessary to maintain joint progenitor cell fate. Dev Cell 16(5):734-43. [PubMed: 19460349]  [MGI Ref ID J:148688]

Kaul A; Koster M; Neuhaus H; Braun T. 2000. Myf-5 revisited: loss of early myotome formation does not lead to a rib phenotype in homozygous Myf-5 mutant mice. Cell 102(1):17-9. [PubMed: 10929709]  [MGI Ref ID J:63517]

Mielcarek M; Piotrowska I; Schneider A; Gunther S; Braun T. 2009. VITO-2, a new SID domain protein, is expressed in the myogenic lineage during early mouse embryonic development. Gene Expr Patterns 9(3):129-37. [PubMed: 19118645]  [MGI Ref ID J:144198]

Moncaut N; Cross JW; Siligan C; Keith A; Taylor K; Rigby PW; Carvajal JJ. 2012. Musculin and TCF21 coordinate the maintenance of myogenic regulatory factor expression levels during mouse craniofacial development. Development 139(5):958-67. [PubMed: 22318627]  [MGI Ref ID J:182749]

Reddy T; Kablar B. 2004. Evidence for the involvement of neurotrophins in muscle transdifferentiation and acetylcholine receptor transformation in the esophagus of Myf5(-/-):MyoD(-/-) and NT-3(-/-) embryos. Dev Dyn 231(4):683-92. [PubMed: 15497153]  [MGI Ref ID J:93814]

Rudnicki MA; Schnegelsberg PN; Stead RH; Braun T; Arnold HH; Jaenisch R. 1993. MyoD or Myf-5 is required for the formation of skeletal muscle. Cell 75(7):1351-9. [PubMed: 8269513]  [MGI Ref ID J:16387]

Stephens HE; Belliveau AC; Gupta JS; Mirkovic S; Kablar B. 2005. The role of neurotrophins in the maintenance of the spinal cord motor neurons and the dorsal root ganglia proprioceptive sensory neurons. Int J Dev Neurosci 23(7):613-20. [PubMed: 16183241]  [MGI Ref ID J:106341]

Tallquist MD; Weismann KE; Hellstrom M; Soriano P. 2000. Early myotome specification regulates PDGFA expression and axial skeleton development Development 127(23):5059-70. [PubMed: 11060232]  [MGI Ref ID J:65301]

Wang Y; Schnegelsberg PN; Dausman J; Jaenisch R. 1996. Functional redundancy of the muscle-specific transcription factors Myf5 and myogenin. Nature 379(6568):823-5. [PubMed: 8587605]  [MGI Ref ID J:31611]

Wilson-Rawls J; Hurt CR; Parsons SM; Rawls A. 1999. Differential regulation of epaxial and hypaxial muscle development by paraxis. Development 126(23):5217-29. [PubMed: 10556048]  [MGI Ref ID J:44659]

Yoon JK; Olson EN; Arnold HH; Wold BJ. 1997. Different MRF4 knockout alleles differentially disrupt Myf-5 expression: cis-regulatory interactions at the MRF4/Myf-5 locus. Dev Biol 188(2):349-62. [PubMed: 9268580]  [MGI Ref ID J:42453]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000651 BALB/cJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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