Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Background Strain BALB/c Donor Strain 129S4 via J1 ES cell line Generation N?+N12pN5p (25-JAN-04)   Donating Investigator Rudolf Jaenisch,   Massachusetts Institute of Technology

Description
Mice homozygous for a targeted mutation in the Myf5 gene die perinatally from respiratory failure due to improper development of the rib cage. Ribs are truncated and the sternum is shortened, with no sternebral segmentation and no connection to the ribs. Mutant mice show no obvious alterations in the vertebral column in comparison to wild type controls, and there are no skeletal muscle abnormalities apparent at birth.

Control Information

	Control
	Wild-type from the colony
	000651 BALB/cJ
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Myf5

007893	B6.129S4-Myf5tm3(cre)Sor/J
007845	B6;129S4-Myf5tm3(cre)Sor/J

View Strains carrying other alleles of Myf5     (2 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Myf5^tm1Jae/Myf5^tm1Jae

        involves: 129S4/SvJae

• skeleton phenotype
• abnormal rib-sternum attachment (MGI Ref ID J:24360)
  • no contacts with the sternum are made because the ribs are short
• abnormal sternum ossification (MGI Ref ID J:24360)
  • sternum is completely ossified
• rib bifurcation (MGI Ref ID J:42453)
• short ribs (MGI Ref ID J:24360)
  • mutants lack the distal parts of the ribs; ribs are shorter than in Myf6 homozygotes
• muscle phenotype
• abnormal myotome development (MGI Ref ID J:42453)
  • no myotomes are detected at E10.5

Myf5^tm1Jae/Myf5^tm1Jae

        either: 129-Myf5^tm1Jae or (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)

• lethality-prenatal/perinatal
• neonatal lethality (MGI Ref ID J:3013)
  • die immediately after birth
• respiratory system phenotype
• respiratory failure (MGI Ref ID J:3013)
  • unable to breathe
• skeleton phenotype
• abnormal rib-sternum attachment (MGI Ref ID J:3013)
  • ribs do not attach to the sternum due to their truncation
• abnormal sternum ossification (MGI Ref ID J:3013)
  • the sternum is completely ossified without sternebral segmentation
• short ribs (MGI Ref ID J:3013)
  • absence of the major distal part of the ribs; the cranial rib stumps on both sides of the vertebral column end in dilated enlarged structures
• short sternum (MGI Ref ID J:3013)
• embryogenesis phenotype
• abnormal somite development (MGI Ref ID J:3013)
  • appearance of myotomal cells in early somites is delayed by several days
• muscle phenotype
• abnormal muscle morphology (MGI Ref ID J:3013)
  • organization of muscles in the anterior thoracic wall is disorganized, probably due to lack of ribs
• abnormal myotome development (MGI Ref ID J:3013)
  • muscle-specific marker analysis indicates a delay in the development of the myotome

Myf5^tm1Jae/Myf5^tm1Jae

        involves: 129S4/SvJae * C57BL/6J

• skeleton phenotype
• fusion of vertebral arches (MGI Ref ID J:65301)
  • animals show extensive vertebral arch fusions compared to wild-type

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Myf5^tm1Jae related

Developmental Biology Research
Mesodermal Defects (Myogenesis Defects)
Skeletal Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Myf5tm1Jae
Allele Name		targeted mutation 1, Rudolf Jaenisch
Allele Type		Targeted (knock-out)
Common Name(s)		Myf-5 (-); Myf-5m1; Myf5Jae;
Mutation Made By		Rudolf Jaenisch,   Massachusetts Institute of Technology
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Myf5, myogenic factor 5
Chromosome		10
Gene Common Name(s)		B130010J22Rik; Myf-5; RIKEN cDNA B130010J22 gene; bHLHc2;
General Note		Mice homozygous for Myf6 mutations lack or show reduced Myf5 expression and exhibit phenotypes seen in Myf5tm1Jae homozygotes. Studies have shown that Myf6 may regulate Myf5 expression by a cis-acting mechanism and that the skeletal abnormalities seen in Myf6 homozygotes may result from a decrease or lack of Myf5 expression (J:24360, J:31636).
Molecular Note		A PGK-neomycin cassette was inserted into exon 1 of the gene, disrupting the reading frame. [MGI Ref ID J:3013]

Genotyping

Genotyping Information

Genotyping Protocols

Myf-5^tm1Jae, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Braun T; Rudnicki MA; Arnold HH; Jaenisch R. 1992. Targeted inactivation of the muscle regulatory gene Myf-5 results in abnormal rib development and perinatal death. Cell 71(3):369-82. [PubMed: 1423602]  [MGI Ref ID J:3013]

Additional References

Rudnicki MA; Schnegelsberg PN; Stead RH; Braun T; Arnold HH; Jaenisch R. 1993. MyoD or Myf-5 is required for the formation of skeletal muscle. Cell 75(7):1351-9. [PubMed: 8269513]  [MGI Ref ID J:16387]

Myf5^tm1Jae related

Angka HE; Kablar B. 2007. Differential responses to the application of exogenous NT-3 are observed for subpopulations of motor and sensory neurons depending on the presence of skeletal muscle. Dev Dyn 236(5):1193-202. [PubMed: 17436272]  [MGI Ref ID J:121117]

Baguma-Nibasheka M; Angka HE; Inanlou MR; Kablar B. 2007. Microarray analysis of Myf5-/-:MyoD-/- hypoplastic mouse lungs reveals a profile of genes involved in pneumocyte differentiation. Histol Histopathol 22(5):483-95. [PubMed: 17330803]  [MGI Ref ID J:121955]

Bidwell MC; Eitzman BA; Walmer DK; McLachlan JA; Gray KD. 1995. Analysis of messenger ribonucleic acid and protein for the ligands and receptors of the platelet-derived growth factor signaling pathway in the placenta, extraembryonic membranes, and uterus during the latter half of murine gestation. Endocrinology 136(11):5189-201. [PubMed: 7588258]  [MGI Ref ID J:29762]

Braun T; Arnold HH. 1995. Inactivation of Myf-6 and Myf-5 genes in mice leads to alterations in skeletal muscle development. EMBO J 14(6):1176-86. [PubMed: 7720708]  [MGI Ref ID J:24360]

Braun T; Bober E; Rudnicki MA; Jaenisch R; Arnold HH. 1994. MyoD expression marks the onset of skeletal myogenesis in Myf-5 mutant mice. Development 120(11):3083-92. [PubMed: 7720554]  [MGI Ref ID J:21616]

Chen JC; Goldhamer DJ. 2004. The core enhancer is essential for proper timing of MyoD activation in limb buds and branchial arches. Dev Biol 265(2):502-12. [PubMed: 14732408]  [MGI Ref ID J:87370]

Floss T; Arnold HH; Braun T. 1996. Myf-5(m1)/Myf-6(m1) compound heterozygous mouse mutants down-regulate Myf-5 expression and exert rib defects: evidence for long-range cis effects on Myf-5 transcription. Dev Biol 174(1):140-7. [PubMed: 8626014]  [MGI Ref ID J:31636]

Garry DJ; Yang Q; Bassel-Duby R; Williams RS. 1997. Persistent expression of MNF identifies myogenic stem cells in postnatal muscles. Dev Biol 188(2):280-94. [PubMed: 9268575]  [MGI Ref ID J:42845]

Gomez C; David V; Peet NM; Vico L; Chenu C; Malaval L; Skerry TM. 2007. Absence of mechanical loading in utero influences bone mass and architecture but not innervation in Myod-Myf5-deficient mice. J Anat 210(3):259-71. [PubMed: 17331176]  [MGI Ref ID J:124478]

Grass S; Arnold HH; Braun T. 1996. Alterations in somite patterning of Myf-5-deficient mice: a possible role for FGF-4 and FGF-6. Development 122(1):141-50. [PubMed: 8565825]  [MGI Ref ID J:30824]

Inanlou MR; Kablar B. 2005. Abnormal development of the intercostal muscles and the rib cage in Myf5-/- embryos leads to pulmonary hypoplasia. Dev Dyn 232(1):43-54. [PubMed: 15580568]  [MGI Ref ID J:95138]

Inanlou MR; Kablar B. 2005. Contractile activity of skeletal musculature involved in breathing is essential for normal lung cell differentiation, as revealed in Myf5-/-:MyoD-/- embryos. Dev Dyn 233(3):772-782. [PubMed: 15844178]  [MGI Ref ID J:98811]

Kablar B. 2003. Determination of retinal cell fates is affected in the absence of extraocular striated muscles. Dev Dyn 226(3):478-90. [PubMed: 12619134]  [MGI Ref ID J:82209]

Kablar B; Asakura A; Krastel K; Ying C; May LL; Goldhamer DJ ; Rudnicki MA. 1998. MyoD and Myf-5 define the specification of musculature of distinct embryonic origin. Biochem Cell Biol 76(6):1079-91. [PubMed: 10392718]  [MGI Ref ID J:55565]

Kablar B; Belliveau AC. 2005. Presence of neurotrophic factors in skeletal muscle correlates with survival of spinal cord motor neurons. Dev Dyn 234(3):659-69. [PubMed: 16193506]  [MGI Ref ID J:102289]

Kablar B; Krastel K; Tajbakhsh S; Rudnicki MA. 2003. Myf5 and MyoD activation define independent myogenic compartments during embryonic development. Dev Biol 258(2):307-18. [PubMed: 12798290]  [MGI Ref ID J:83932]

Kablar B; Krastel K; Ying C; Asakura A; Tapscott SJ; Rudnicki MA. 1997. MyoD and Myf-5 differentially regulate the development of limb versus trunk skeletal muscle. Development 124(23):4729-38. [PubMed: 9428409]  [MGI Ref ID J:45349]

Kablar B; Rudnicki MA. 1999. Development in the absence of skeletal muscle results in the sequential ablation of motor neurons from the spinal cord to the brain. Dev Biol 208(1):93-109. [PubMed: 10075844]  [MGI Ref ID J:54064]

Kablar B; Rudnicki MA. 2002. Information provided by the skeletal muscle and associated neurons is necessary for proper brain development. Int J Dev Neurosci 20(7):573-84. [PubMed: 12485625]  [MGI Ref ID J:100037]

Reddy T; Kablar B. 2004. Evidence for the involvement of neurotrophins in muscle transdifferentiation and acetylcholine receptor transformation in the esophagus of Myf5(-/-):MyoD(-/-) and NT-3(-/-) embryos. Dev Dyn 231(4):683-92. [PubMed: 15497153]  [MGI Ref ID J:93814]

Rudnicki MA; Schnegelsberg PN; Stead RH; Braun T; Arnold HH; Jaenisch R. 1993. MyoD or Myf-5 is required for the formation of skeletal muscle. Cell 75(7):1351-9. [PubMed: 8269513]  [MGI Ref ID J:16387]

Stephens HE; Belliveau AC; Gupta JS; Mirkovic S; Kablar B. 2005. The role of neurotrophins in the maintenance of the spinal cord motor neurons and the dorsal root ganglia proprioceptive sensory neurons. Int J Dev Neurosci 23(7):613-20. [PubMed: 16183241]  [MGI Ref ID J:106341]

Tallquist MD; Weismann KE; Hellstrom M; Soriano P. 2000. Early myotome specification regulates PDGFA expression and axial skeleton development Development 127(23):5059-70. [PubMed: 11060232]  [MGI Ref ID J:65301]

Wang Y; Schnegelsberg PN; Dausman J; Jaenisch R. 1996. Functional redundancy of the muscle-specific transcription factors Myf5 and myogenin. Nature 379(6568):823-5. [PubMed: 8587605]  [MGI Ref ID J:31611]

Wilson-Rawls J; Hurt CR; Parsons SM; Rawls A. 1999. Differential regulation of epaxial and hypaxial muscle development by paraxis. Development 126(23):5217-29. [PubMed: 10556048]  [MGI Ref ID J:44659]

Yoon JK; Olson EN; Arnold HH; Wold BJ. 1997. Different MRF4 knockout alleles differentially disrupt Myf-5 expression: cis-regulatory interactions at the MRF4/Myf-5 locus. Dev Biol 188(2):349-62. [PubMed: 9268580]  [MGI Ref ID J:42453]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Wild-type from the colony
	000651 BALB/cJ
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

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For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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