Description

Strain Information

Former Names FVB/N-Tg(ANX6)56Agh/J    (Changed: 07-AUG-08 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation N?+1pN1   Donating Investigator Ann-Marie Gunteski-Hamblin,   Univ of Cincinnati College of Medicine

Description
Mice carrying the human annexin VI transgene display a phenotype similar to congestive heart failure. They show alterations in the contractility and calcium dynamics in cardiomyocytes, and these cells also have reduced basal calcium levels. Mice homozygous for the transgene die by 4 weeks of age while hemizygous mice live to about 1 year of age.

Control Information

	Control
	Noncarrier
	001800 FVB/NJ
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Myh6

002640	B6;SJL-Tg(Myh6-ADRBK1)27Wjk/J
002637	B6SJL-Tg(CAMalpha1b)7Wjk/J
002639	B6SJL-Tg(Myh6-ADRBK1)12Wjk/J
002638	B6SJL-Tg(WTbeta2)4Wjk/J
006768	D2.Cg-Tg(Myh6-Zfpm2)1Sho/EiJ

View Strains carrying other alleles of Myh6     (5 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Tg(ANX6)2Agh/0

        FVB/N-Tg(ANX6)2Agh

• cardiovascular system phenotype
• abnormal cardiovascular system morphology (MGI Ref ID J:92579)
• abnormal pulmonary vein morphology (MGI Ref ID J:92579)
  • myocytes in the pulmonary vein muscular wall are replaced with fibrous tissue
• cardiac fibrosis (MGI Ref ID J:92579)
  • mice exhibit mild heart lesions with multifocally extensive and diffuse fibrosis in the ventricles and atria that worsens with age
  • mice exhibit occasional mineralization associated with fibrosis
  • the myocardium and endocardium of the ventricles are most affected by fibrosis
• dilated atria (MGI Ref ID J:92579)
  • mice exhibit dilation of the atria with large thrombi that partially occludes the lumen
• dilated right ventricle (MGI Ref ID J:92579)
• myocardial necrosis (MGI Ref ID J:92579)
• right ventricle hypertrophy (MGI Ref ID J:92579)
• abnormal cardiovascular system physiology (MGI Ref ID J:92579)
• abnormal blood circulation (MGI Ref ID J:92579)
  • mice exhibit an increase in chamber turbulence or pooling in the heart
• abnormal myocardial fiber physiology (MGI Ref ID J:92579)
  • cardiomyocytes exhibit a 40% lower basal level of free calcium ions and after stimulation a lower calcium ion peak compared to in wild-type mice
  • however, depression of base levels of intracellular calcium ions is normal following stimulation
• cardiomyopathy (MGI Ref ID J:92579)
  • heart function deteriorates with age
• decreased cardiac muscle contractility (MGI Ref ID J:92579)
  • cardiomyocytes exhibit decreased percent shortening, rate of contraction and rate of relaxation compared to wild-type mice
• myocarditis (MGI Ref ID J:92579)
  • mice exhibit myocyte necrosis, mild inflammation and occasional mineralization associated with fibrosis
• hematopoietic system phenotype
• decreased thymus cortex area (MGI Ref ID J:92579)
  • in some mice
• thymic cortex hypoplasia (MGI Ref ID J:92579)
  • in some mice
• immune system phenotype
• decreased thymus cortex area (MGI Ref ID J:92579)
  • in some mice
• myocarditis (MGI Ref ID J:92579)
  • mice exhibit myocyte necrosis, mild inflammation and occasional mineralization associated with fibrosis
• thymic cortex hypoplasia (MGI Ref ID J:92579)
  • in some mice
• muscle phenotype
• cardiomyopathy (MGI Ref ID J:92579)
  • heart function deteriorates with age
• decreased cardiac muscle contractility (MGI Ref ID J:92579)
  • cardiomyocytes exhibit decreased percent shortening, rate of contraction and rate of relaxation compared to wild-type mice
• myocardial necrosis (MGI Ref ID J:92579)

Tg(ANX6)2Agh/Tg(ANX6)2Agh

        FVB/N-Tg(ANX6)2Agh

• lethality-postnatal
• postnatal lethality (MGI Ref ID J:92579)
  • mice die prior to weaning
• cardiovascular system phenotype
• abnormal heart morphology (MGI Ref ID J:92579)
  • mice exhibit severe hearth pathology and die prior to weaning

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cardiovascular Research
Heart Abnormalities

Internal/Organ Research
Heart Abnormalities

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(ANX6)2Agh
Allele Name		transgene insertion 2, Ann-Marie Gunteski-Hamblin
Allele Type		Transgenic (random, expressed)
Common Name(s)		Tg(ANX6)56Agh;
Mutation Made By		Ann-Marie Gunteski-Hamblin,   Univ of Cincinnati College of Medicine
Strain of Origin		FVB/N
Expressed Gene		ANXA6, annexin A6, human
Promoter		Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, mouse, laboratory
Molecular Note		The transgene contains the myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter and the human annexin VI gene. Several lines were created inluding lines 11, 6, 4 and 2. [MGI Ref ID J:92579]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(ANX6)2Agh, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Gunteski-Hamblin AM; Song G; Walsh RA; Frenzke M; Boivin GP; Dorn GW 2nd; Kaetzel MA; Horseman ND; Dedman JR. 1996. Annexin VI overexpression targeted to heart alters cardiomyocyte function in transgenic mice. Am J Physiol 270(3 Pt 2):H1091-100. [PubMed: 8780208]  [MGI Ref ID J:92579]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Noncarrier
	001800 FVB/NJ
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.