Strain Name:

B6;129S-Btktm1Wk/J

Stock Number:

002536

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Availability:

Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Frederick W. Alt,   Children's Hospital

Appearance
white-bellied agouti
Related Genotype: Aw/?

black
Related Genotype: a/a

Description
Mice homozygous for the Btktm1Wk targeted mutation are viable and fertile. Homozygous mutant mice show defects in B-cell maturation and activation. This strain has the same phenotype as the spontaneous X-linked immune deficiency mutation (CBA/CaHN-Btkxid/J; Stock No. 001011). See Inbred Strain listing.

Development
Exons 13 and 14 of the gene, encoding the last 20 amino acids of the SH2 domain, and the first two exons of the kinase domain were deleted and replaced with neomycin. This deletion also disrupted the open reading frame and introduced a stop codon. No immunoreactive protein was detected in homozygous targeted mice.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Btk
009361   B6.CBA-Btkxid/AllmJ
001011   CBA/CaHN-Btkxid/J
View Strains carrying other alleles of Btk     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
Agammaglobulinemia, X-Linked; XLA
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Isolated Growth Hormone Deficiency, Type III; IGHD3   (BTK)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Btktm1Wk/Btktm1Wk

        involves: 129S4/SvJae * C57BL/6
  • hematopoietic system phenotype
  • abnormal B cell differentiation
    • partial arrest of B cell development   (MGI Ref ID J:28968)
    • abnormal B cell activation
      • defective B cell activation after immunization with T-independent type II antigens   (MGI Ref ID J:28968)
      • decreased B cell proliferation
        • in vitro, B cells show a reduced level of proliferation in response to LPS compared to wild-type   (MGI Ref ID J:82298)
    • increased pro-B cell number
      • increased numbers of pro-B cells   (MGI Ref ID J:28968)
  • decreased IgG3 level   (MGI Ref ID J:82298)
    • decreased serum IgG3 concentration   (MGI Ref ID J:28968)
  • decreased IgM level   (MGI Ref ID J:82298)
    • decreased serum IgM concentration   (MGI Ref ID J:28968)
  • decreased mature B cell number
    • decreased numbers of mature B cells   (MGI Ref ID J:28968)
    • numbers of mature recirculating B cells (B220highIgM+, HSAlowIgM+, and B220+IgD+) are reduced   (MGI Ref ID J:82298)
  • decreased spleen germinal center number
    • germinal centers are infrequent in TNP-LPS immunized mutants   (MGI Ref ID J:82298)
  • decreased spleen germinal center size
    • germinal centers are smaller in TNP-LPS immunized mutants   (MGI Ref ID J:82298)
  • immune system phenotype
  • abnormal B cell differentiation
    • partial arrest of B cell development   (MGI Ref ID J:28968)
    • abnormal B cell activation
      • defective B cell activation after immunization with T-independent type II antigens   (MGI Ref ID J:28968)
      • decreased B cell proliferation
        • in vitro, B cells show a reduced level of proliferation in response to LPS compared to wild-type   (MGI Ref ID J:82298)
    • increased pro-B cell number
      • increased numbers of pro-B cells   (MGI Ref ID J:28968)
  • abnormal lymph node germinal center morphology
    • smaller and fewer germinal centers are observed in response to TI-I antigen, TNP-LPS   (MGI Ref ID J:82298)
  • decreased IgG3 level   (MGI Ref ID J:82298)
    • decreased serum IgG3 concentration   (MGI Ref ID J:28968)
  • decreased IgM level   (MGI Ref ID J:82298)
    • decreased serum IgM concentration   (MGI Ref ID J:28968)
  • decreased mature B cell number
    • decreased numbers of mature B cells   (MGI Ref ID J:28968)
    • numbers of mature recirculating B cells (B220highIgM+, HSAlowIgM+, and B220+IgD+) are reduced   (MGI Ref ID J:82298)
  • decreased spleen germinal center number
    • germinal centers are infrequent in TNP-LPS immunized mutants   (MGI Ref ID J:82298)
  • decreased spleen germinal center size
    • germinal centers are smaller in TNP-LPS immunized mutants   (MGI Ref ID J:82298)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Btktm1Wk related

Hematological Research
Immunological Defects
Mast Cell Deficiency

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      B cell deficiency
Inflammation

Research Tools
Apoptosis Research
Cancer Research
      B cell deficiency
Immunology, Inflammation and Autoimmunity Research
      B cell deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Btktm1Wk
Allele Name targeted mutation 1, Wasif Kahn
Allele Type Targeted (Null/Knockout)
Common Name(s) Btk-; Btk-kin-/GL; BtkM;
Mutation Made ByDr. Wasif Khan,   Children's Hospital
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Btk, Bruton agammaglobulinemia tyrosine kinase
Chromosome X
Gene Common Name(s) AGMX1; AI528679; AT; ATK; BPK; Bruton's tyrosine kinase; IMD1; PSCTK1; X-linked immune deficiency; XLA; expressed sequence AI528679; xid;
Molecular Note A genomic fragment containing exons 13 and 14 was replaced with a neomycin resistance cassette. These sequences encode the beginning of the kinase domain. [MGI Ref ID J:28968]

Genotyping

Genotyping Information

Genotyping Protocols

Btktm1Wk, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Khan WN; Sideras P; Rosen FS; Alt FW. 1995. The role of Bruton's tyrosine kinase in B-cell development and function in mice and man. Ann N Y Acad Sci 764:27-38. [PubMed: 7486535]  [MGI Ref ID J:109912]

Additional References

Merchant M; Longnecker R. 2001. LMP2A survival and developmental signals are transmitted through Btk-dependent and Btk-independent pathways. Virology 291(1):46-54. [PubMed: 11878875]  [MGI Ref ID J:74306]

Btktm1Wk related

Alugupalli KR; Akira S; Lien E; Leong JM. 2007. MyD88- and Bruton's tyrosine kinase-mediated signals are essential for T cell-independent pathogen-specific IgM responses. J Immunol 178(6):3740-9. [PubMed: 17339472]  [MGI Ref ID J:144277]

Atkinson BT; Ellmeier W; Watson SP. 2003. Tec regulates platelet activation by GPVI in the absence of Btk. Blood 102(10):3592-9. [PubMed: 12842985]  [MGI Ref ID J:86448]

Bao Y; Zheng J; Han C; Jin J; Han H; Liu Y; Lau YL; Tu W; Cao X. 2012. Tyrosine kinase Btk is required for NK cell activation. J Biol Chem 287(28):23769-78. [PubMed: 22589540]  [MGI Ref ID J:188371]

Bhattacharyya S; Deb J; Patra AK; Thuy Pham DA; Chen W; Vaeth M; Berberich-Siebelt F; Klein-Hessling S; Lamperti ED; Reifenberg K; Jellusova J; Schweizer A; Nitschke L; Leich E; Rosenwald A; Brunner C; Engelmann S; Bommhardt U; Avots A; Muller MR; Kondo E; Serfling E. 2011. NFATc1 affects mouse splenic B cell function by controlling the calcineurin--NFAT signaling network. J Exp Med 208(4):823-39. [PubMed: 21464221]  [MGI Ref ID J:177319]

Castro I; Wright JA; Damdinsuren B; Hoek KL; Carlesso G; Shinners NP; Gerstein RM; Woodland RT; Sen R; Khan WN. 2009. B cell receptor-mediated sustained c-Rel activation facilitates late transitional B cell survival through control of B cell activating factor receptor and NF-kappaB2. J Immunol 182(12):7729-37. [PubMed: 19494297]  [MGI Ref ID J:149292]

Colombo MJ; Alugupalli KR. 2008. Complement Factor H-Binding Protein, a Putative Virulence Determinant of Borrelia hermsii, Is an Antigenic Target for Protective B1b Lymphocytes. J Immunol 180(7):4858-64. [PubMed: 18354209]  [MGI Ref ID J:133379]

Contreras CM; Halcomb KE; Randle L; Hinman RM; Gutierrez T; Clarke SH; Satterthwaite AB. 2007. Btk regulates multiple stages in the development and survival of B-1 cells. Mol Immunol 44(10):2719-28. [PubMed: 17207856]  [MGI Ref ID J:118704]

Ellmeier W; Jung S; Sunshine MJ; Hatam F; Xu Y; Baltimore D; Mano H; Littman DR. 2000. Severe B cell deficiency in mice lacking the tec kinase family members tec and Btk J Exp Med 192(11):1611-24. [PubMed: 11104803]  [MGI Ref ID J:66080]

Esplin BL; Welner RS; Zhang Q; Borghesi LA; Kincade PW. 2009. A differentiation pathway for B1 cells in adult bone marrow. Proc Natl Acad Sci U S A 106(14):5773-8. [PubMed: 19307589]  [MGI Ref ID J:147564]

Forssell J; Sideras P; Eriksson C; Malm-Erjefalt M; Rydell-Tormanen K; Ericsson PO; Erjefalt JS. 2005. Interleukin-2-inducible T cell kinase regulates mast cell degranulation and acute allergic responses. Am J Respir Cell Mol Biol 32(6):511-20. [PubMed: 15778496]  [MGI Ref ID J:110944]

Guo S; Wahl MI; Witte ON. 2006. Mutational analysis of the SH2-kinase linker region of Bruton's tyrosine kinase defines alternative modes of regulation for cytoplasmic tyrosine kinase families. Int Immunol 18(1):79-87. [PubMed: 16291652]  [MGI Ref ID J:104167]

Gutierrez T; Halcomb KE; Coughran AJ; Li QZ; Satterthwaite AB. 2010. Separate checkpoints regulate splenic plasma cell accumulation and IgG autoantibody production in Lyn-deficient mice. Eur J Immunol 40(7):1897-905. [PubMed: 20394076]  [MGI Ref ID J:165938]

Gutierrez T; Mayeux JM; Ortega SB; Karandikar NJ; Li QZ; Rakheja D; Zhou XJ; Satterthwaite AB. 2013. IL-21 promotes the production of anti-DNA IgG but is dispensable for kidney damage in lyn(-/-) mice. Eur J Immunol 43(2):382-93. [PubMed: 23169140]  [MGI Ref ID J:192815]

Habib T; Park H; Tsang M; de Alboran IM; Nicks A; Wilson L; Knoepfler PS; Andrews S; Rawlings DJ; Eisenman RN; Iritani BM. 2007. Myc stimulates B lymphocyte differentiation and amplifies calcium signaling. J Cell Biol 179(4):717-31. [PubMed: 17998397]  [MGI Ref ID J:135554]

Halcomb KE; Contreras CM; Hinman RM; Coursey TG; Wright HL; Satterthwaite AB. 2007. Btk and phospholipase Cgamma2 can function independently during B cell development. Eur J Immunol 37(4):1033-42. [PubMed: 17372989]  [MGI Ref ID J:120843]

Halcomb KE; Musuka S; Gutierrez T; Wright HL; Satterthwaite AB. 2008. Btk regulates localization, in vivo activation, and class switching of anti-DNA B cells. Mol Immunol :. [PubMed: 18849077]  [MGI Ref ID J:141113]

Hasan M; Lopez-Herrera G; Blomberg KE; Lindvall JM; Berglof A; Smith CI; Vargas L. 2008. Defective Toll-like receptor 9-mediated cytokine production in B cells from Bruton's tyrosine kinase-deficient mice. Immunology 123(2):239-49. [PubMed: 17725607]  [MGI Ref ID J:134805]

Hayer S; Polzer K; Brandl A; Zwerina J; Kireva T; Smolen JS; Schett G. 2008. B-cell infiltrates induce endosteal bone formation in inflammatory arthritis. J Bone Miner Res 23(10):1650-60. [PubMed: 18505368]  [MGI Ref ID J:153412]

Hinman RM; Bushanam JN; Nichols WA; Satterthwaite AB. 2007. B cell receptor signaling down-regulates forkhead box transcription factor class O 1 mRNA expression via phosphatidylinositol 3-kinase and Bruton's tyrosine kinase. J Immunol 178(2):740-7. [PubMed: 17202334]  [MGI Ref ID J:142634]

Ho LH; Uehara T; Chen CC; Kubagawa H; Cooper MD. 1999. Constitutive tyrosine phosphorylation of the inhibitory paired Ig-like receptor PIR-B. Proc Natl Acad Sci U S A 96(26):15086-90. [PubMed: 10611342]  [MGI Ref ID J:119839]

Hoek KL; Carlesso G; Clark ES; Khan WN. 2009. Absence of mature peripheral B cell populations in mice with concomitant defects in B cell receptor and BAFF-R signaling. J Immunol 183(9):5630-43. [PubMed: 19843948]  [MGI Ref ID J:156793]

Iwaki S; Tkaczyk C; Satterthwaite AB; Halcomb K; Beaven MA; Metcalfe DD; Gilfillan AM. 2005. Btk plays a crucial role in the amplification of Fc epsilonRI-mediated mast cell activation by kit. J Biol Chem 280(48):40261-70. [PubMed: 16176929]  [MGI Ref ID J:132405]

Iyer AS; Morales JL; Huang W; Ojo F; Ning G; Wills E; Baines JD; August A. 2011. Absence of Tec Family Kinases Interleukin-2 Inducible T cell Kinase (Itk) and Bruton's Tyrosine Kinase (Btk) Severely Impairs Fc{epsilon}RI-dependent Mast Cell Responses. J Biol Chem 286(11):9503-13. [PubMed: 21212279]  [MGI Ref ID J:170527]

Jumaa H; Mitterer M; Reth M; Nielsen PJ. 2001. The absence of SLP65 and Btk blocks B cell development at the preB cell receptor-positive stage. Eur J Immunol 31(7):2164-9. [PubMed: 11449370]  [MGI Ref ID J:70406]

Karlsson MC; Guinamard R; Bolland S; Sankala M; Steinman RM; Ravetch JV. 2003. Macrophages control the retention and trafficking of B lymphocytes in the splenic marginal zone. J Exp Med 198(2):333-40. [PubMed: 12874264]  [MGI Ref ID J:119256]

Kawakami Y; Inagaki N; Salek-Ardakani S; Kitaura J; Tanaka H; Nagao K; Kawakami Y; Xiao W; Nagai H; Croft M; Kawakami T. 2006. Regulation of dendritic cell maturation and function by Bruton's tyrosine kinase via IL-10 and Stat3. Proc Natl Acad Sci U S A 103(1):153-8. [PubMed: 16371463]  [MGI Ref ID J:104554]

Kawakami Y; Kitaura J; Hartman SE; Lowell CA; Siraganian RP; Kawakami T. 2000. Regulation of protein kinase CbetaI by two protein-tyrosine kinases, Btk and Syk. Proc Natl Acad Sci U S A 97(13):7423-8. [PubMed: 10852954]  [MGI Ref ID J:126402]

Kawakami Y; Kitaura J; Satterthwaite AB; Kato RM; Asai K; Hartman SE; Maeda-Yamamoto M; Lowell CA; Rawlings DJ; Witte ON; Kawakami T. 2000. Redundant and opposing functions of two tyrosine kinases, Btk and Lyn, in mast cell activation. J Immunol 165(3):1210-9. [PubMed: 10903718]  [MGI Ref ID J:120482]

Khan WN; Alt FW; Gerstein RM; Malynn BA; Larsson I; Rathbun G; Davidson L; Muller S; Kantor AB; Herzenberg LA; Rosen FS; Sideras P. 1995. Defective B cell development and function in Btk-deficient mice. Immunity 3(3):283-99. [PubMed: 7552994]  [MGI Ref ID J:28968]

Khan WN; Nilsson A; Mizoguchi E; Castigli E; Forsell J; Bhan AK; Geha R; Sideras P; Alt FW. 1997. Impaired B cell maturation in mice lacking Bruton's tyrosine kinase (Btk) and CD40. Int Immunol 9(3):395-405. [PubMed: 9088978]  [MGI Ref ID J:82298]

Kitaura J; Eto K; Kinoshita T; Kawakami Y; Leitges M; Lowell CA; Kawakami T. 2005. Regulation of highly cytokinergic IgE-induced mast cell adhesion by Src, Syk, Tec, and protein kinase C family kinases. J Immunol 174(8):4495-504. [PubMed: 15814670]  [MGI Ref ID J:98164]

Kneidinger M; Schmidt U; Rix U; Gleixner KV; Vales A; Baumgartner C; Lupinek C; Weghofer M; Bennett KL; Herrmann H; Schebesta A; Thomas WR; Vrtala S; Valenta R; Lee FY; Ellmeier W; Superti-Furga G; Valent P. 2008. The effects of dasatinib on IgE receptor-dependent activation and histamine release in human basophils. Blood 111(6):3097-107. [PubMed: 18180381]  [MGI Ref ID J:132738]

Koprulu AD; Kastner R; Wienerroither S; Lassnig C; Putz EM; Majer O; Reutterer B; Sexl V; Kuchler K; Muller M; Decker T; Ellmeier W. 2013. The tyrosine kinase Btk regulates the macrophage response to Listeria monocytogenes infection. PLoS One 8(3):e60476. [PubMed: 23544144]  [MGI Ref ID J:199894]

Kuehn HS; Swindle EJ; Kim MS; Beaven MA; Metcalfe DD; Gilfillan AM. 2008. The phosphoinositide 3-kinase-dependent activation of Btk is required for optimal eicosanoid production and generation of reactive oxygen species in antigen-stimulated mast cells. J Immunol 181(11):7706-12. [PubMed: 19017959]  [MGI Ref ID J:142195]

Lee KG; Xu S; Kang ZH; Huo J; Huang M; Liu D; Takeuchi O; Akira S; Lam KP. 2012. Bruton's tyrosine kinase phosphorylates Toll-like receptor 3 to initiate antiviral response. Proc Natl Acad Sci U S A 109(15):5791-6. [PubMed: 22454496]  [MGI Ref ID J:183537]

Lee KG; Xu S; Wong ET; Tergaonkar V; Lam KP. 2008. Bruton's tyrosine kinase separately regulates NFkappaB p65RelA activation and cytokine interleukin (IL)-10/IL-12 production in TLR9-stimulated B Cells. J Biol Chem 283(17):11189-98. [PubMed: 18276597]  [MGI Ref ID J:136576]

Lindvall JM; Blomberg KE; Berglof A; Yang Q; Smith CI; Islam TC. 2004. Gene expression profile of B cells from Xid mice and Btk knockout mice. Eur J Immunol 34(7):1981-91. [PubMed: 15214046]  [MGI Ref ID J:115493]

Liu X; Zhan Z; Li D; Xu L; Ma F; Zhang P; Yao H; Cao X. 2011. Intracellular MHC class II molecules promote TLR-triggered innate immune responses by maintaining activation of the kinase Btk. Nat Immunol 12(5):416-24. [PubMed: 21441935]  [MGI Ref ID J:171920]

Matsuda S; Mikami Y; Ohtani M; Fujiwara M; Hirata Y; Minowa A; Terauchi Y; Kadowaki T; Koyasu S. 2009. Critical role of class IA PI3K for c-Rel expression in B lymphocytes. Blood 113(5):1037-44. [PubMed: 18927440]  [MGI Ref ID J:144568]

Mecklenbrauker I; Kalled SL; Leitges M; Mackay F; Tarakhovsky A. 2004. Regulation of B-cell survival by BAFF-dependent PKCdelta-mediated nuclear signalling. Nature 431(7007):456-61. [PubMed: 15361883]  [MGI Ref ID J:92667]

Melcher M; Unger B; Schmidt U; Rajantie IA; Alitalo K; Ellmeier W. 2008. Essential roles for the Tec family kinases Tec and Btk in M-CSF receptor signaling pathways that regulate macrophage survival. J Immunol 180(12):8048-56. [PubMed: 18523268]  [MGI Ref ID J:137240]

Merchant M; Longnecker R. 2001. LMP2A survival and developmental signals are transmitted through Btk-dependent and Btk-independent pathways. Virology 291(1):46-54. [PubMed: 11878875]  [MGI Ref ID J:74306]

Mueller H; Stadtmann A; Van Aken H; Hirsch E; Wang D; Ley K; Zarbock A. 2010. Tyrosine kinase Btk regulates E-selectin-mediated integrin activation and neutrophil recruitment by controlling phospholipase C (PLC) gamma2 and PI3Kgamma pathways. Blood 115(15):3118-27. [PubMed: 20167705]  [MGI Ref ID J:160787]

Oldham AL; Miner CA; Wang HC; Webb CF. 2011. The transcription factor Bright plays a role in marginal zone B lymphocyte development and autoantibody production. Mol Immunol 49(1-2):367-79. [PubMed: 21963220]  [MGI Ref ID J:177457]

Ormsby T; Schlecker E; Ferdin J; Tessarz AS; Angelisova P; Koprulu AD; Borte M; Warnatz K; Schulze I; Ellmeier W; Horejsi V; Cerwenka A. 2011. Btk is a positive regulator in the TREM-1/DAP12 signaling pathway. Blood 118(4):936-45. [PubMed: 21659545]  [MGI Ref ID J:174854]

Petro JB; Rahman SM; Ballard DW; Khan WN. 2000. Bruton's tyrosine kinase is required for activation of IkappaB kinase and nuclear factor kappaB in response to B cell receptor engagement. J Exp Med 191(10):1745-54. [PubMed: 10811867]  [MGI Ref ID J:110720]

Pisitkun P; Deane JA; Difilippantonio MJ; Tarasenko T; Satterthwaite AB; Bolland S. 2006. Autoreactive B cell responses to RNA-related antigens due to TLR7 gene duplication. Science 312(5780):1669-72. [PubMed: 16709748]  [MGI Ref ID J:109758]

Qazi KR; Gehrmann U; Domange Jordo E; Karlsson MC; Gabrielsson S. 2009. Antigen-loaded exosomes alone induce Th1-type memory through a B cell-dependent mechanism. Blood 113(12):2673-83. [PubMed: 19176319]  [MGI Ref ID J:146517]

Satterthwaite AB; Cheroutre H; Khan WN; Sideras P; Witte ON. 1997. Btk dosage determines sensitivity to B cell antigen receptor cross-linking. Proc Natl Acad Sci U S A 94(24):13152-7. [PubMed: 9371815]  [MGI Ref ID J:108696]

Satterthwaite AB; Lowell CA; Khan WN; Sideras P; Alt FW; Witte ON. 1998. Independent and opposing roles for Btk and lyn in B and myeloid signaling pathways. J Exp Med 188(5):833-44. [PubMed: 9730885]  [MGI Ref ID J:50288]

Satterthwaite AB; Willis F; Kanchanastit P; Fruman D; Cantley LC; Helgason CD; Humphries RK; Lowell CA; Simon M; Leitges M; Tarakhovsky A; Tedder TF; Lesche R; Wu H; Witte ON. 2000. A sensitized genetic system for the analysis of murine B lymphocyte signal transduction pathways dependent on Bruton's tyrosine kinase. Proc Natl Acad Sci U S A 97(12):6687-92. [PubMed: 10829070]  [MGI Ref ID J:62717]

Schmidt NW; Mayo LD; Donner DB; Kaplan MH. 2006. p53 regulates Btk-dependent B cell proliferation but not differentiation. J Leukoc Biol 79(4):852-9. [PubMed: 16461747]  [MGI Ref ID J:107548]

Schmidt NW; Thieu VT; Mann BA; Ahyi AN; Kaplan MH. 2006. Bruton's tyrosine kinase is required for TLR-induced IL-10 production. J Immunol 177(10):7203-10. [PubMed: 17082638]  [MGI Ref ID J:140621]

Schmidt U; Abramova A; Boucheron N; Eckelhart E; Schebesta A; Bilic I; Kneidinger M; Unger B; Hammer M; Sibilia M; Valent P; Ellmeier W. 2009. The protein tyrosine kinase Tec regulates mast cell function. Eur J Immunol 39(11):3228-3238. [PubMed: 19688741]  [MGI Ref ID J:154200]

Shao B; Yago T; Coghill PA; Klopocki AG; Mehta-D'souza P; Schmidtke DW; Rodgers W; McEver RP. 2012. Signal-dependent slow leukocyte rolling does not require cytoskeletal anchorage of P-selectin glycoprotein ligand-1 (PSGL-1) or integrin alphaLbeta2. J Biol Chem 287(23):19585-98. [PubMed: 22511754]  [MGI Ref ID J:186204]

Shimomura Y; Ogawa A; Kawada M; Sugimoto K; Mizoguchi E; Shi HN; Pillai S; Bhan AK; Mizoguchi A. 2008. A unique B2 B cell subset in the intestine. J Exp Med 205(6):1343-55. [PubMed: 18519649]  [MGI Ref ID J:137039]

Shinohara M; Koga T; Okamoto K; Sakaguchi S; Arai K; Yasuda H; Takai T; Kodama T; Morio T; Geha RS; Kitamura D; Kurosaki T; Ellmeier W; Takayanagi H. 2008. Tyrosine kinases Btk and Tec regulate osteoclast differentiation by linking RANK and ITAM signals. Cell 132(5):794-806. [PubMed: 18329366]  [MGI Ref ID J:135537]

Soriani A; Moran B; de Virgilio M; Kawakami T; Altman A; Lowell C; Eto K; Shattil SJ. 2006. A role for PKCtheta in outside-in alpha(IIb)beta3 signaling. J Thromb Haemost 4(3):648-55. [PubMed: 16460447]  [MGI Ref ID J:135793]

Suzuki H; Matsuda S; Terauchi Y; Fujiwara M; Ohteki T; Asano T; Behrens TW; Kouro T; Takatsu K; Kadowaki T; Koyasu S. 2003. PI3K and Btk differentially regulate B cell antigen receptor-mediated signal transduction. Nat Immunol 4(3):280-6. [PubMed: 12563258]  [MGI Ref ID J:96704]

Tassi I; Cella M; Castro I; Gilfillan S; Khan WN; Colonna M. 2009. Requirement of phospholipase C-gamma2 (PLCgamma2) for Dectin-1-induced antigen presentation and induction of TH1/TH17 polarization. Eur J Immunol 39(5):1369-78. [PubMed: 19404984]  [MGI Ref ID J:148087]

Webb CF; Yamashita Y; Ayers N; Evetts S; Paulin Y; Conley ME; Smith EA. 2000. The transcription factor Bright associates with Bruton's tyrosine kinase, the defective protein in immunodeficiency disease. J Immunol 165(12):6956-65. [PubMed: 11120822]  [MGI Ref ID J:118397]

Whyburn LR; Halcomb KE; Contreras CM; Lowell CA; Witte ON; Satterthwaite AB. 2003. Reduced dosage of Bruton's tyrosine kinase uncouples B cell hyperresponsiveness from autoimmunity in lyn-/- mice. J Immunol 171(4):1850-8. [PubMed: 12902486]  [MGI Ref ID J:84810]

Whyburn LR; Halcomb KE; Contreras CM; Pappu R; Witte ON; Chan AC; Satterthwaite AB. 2003. Haploinsufficiency of B cell linker protein enhances B cell signaling defects in mice expressing a limiting dosage of Bruton's tyrosine kinase. Int Immunol 15(3):383-92. [PubMed: 12618482]  [MGI Ref ID J:82430]

Xu S; Lee KG; Huo J; Kurosaki T; Lam KP. 2007. Combined deficiencies in Bruton tyrosine kinase and phospholipase Cgamma2 arrest B-cell development at a pre-BCR+ stage. Blood 109(8):3377-84. [PubMed: 17164342]  [MGI Ref ID J:145344]

Yago T; Shao B; Miner JJ; Yao L; Klopocki AG; Maeda K; Coggeshall KM; McEver RP. 2010. E-selectin engages PSGL-1 and CD44 through a common signaling pathway to induce integrin alphaLbeta2-mediated slow leukocyte rolling. Blood 116(3):485-94. [PubMed: 20299514]  [MGI Ref ID J:162825]

Yu PW; Tabuchi RS; Kato RM; Astrakhan A; Humblet-Baron S; Kipp K; Chae K; Ellmeier W; Witte ON; Rawlings DJ. 2004. Sustained correction of B-cell development and function in a murine model of X-linked agammaglobulinemia (XLA) using retroviral-mediated gene transfer. Blood 104(5):1281-90. [PubMed: 15142874]  [MGI Ref ID J:92704]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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