Go to JAX^® Mice Query Form

General Terms and Conditions	Additional Licensing and Use Restrictions

Genes & Alleles

Ccnd1;   Ccnd1tm1Wbg;

---

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Robert Weinberg,   Massachusetts Institute of Technology Generation B6N?+F5-10N1

Appearance
white-bellied agouti
Related Genotype: A^w/?

black
Related Genotype: a/a

Strain Description
Mice homozygous for the targeted mutation develop to term but show growth retardation that is most pronounced by three weeks of age. The majority of mutant mice die early in life, often within the first month. Survivors continue to show lower than average weight and increased mortality. Mammary gland epithelial duct development is normal in virgin mutant mice. Mutant females that survive to adulthood are fertile, but mammary glands of pregnant mice show a dramatic impairment in the expansion of alveolar lobes and mice are unable to lactate. Steroid hormone levels are normal, and there is no apparent defect in estrogen receptor number, suggesting that cyclin D1 deficiency has an effect on the target tissue directly. Mutant mice demonstrate a neurological abnormality evidenced by limb retraction when lifted by their tails. The most severely affected animals remain in a clasped, flexed position for a few seconds after they have been returned to their cage. Retinal abnormalities include a dramatic reduction in cell numbers in all cell layers of the neural retina. This is due to the severely reduced ability of mutant retinal cell precursors to proliferate during embryonic development. The thickness of the inner plexiform layer is also reduced. The cells within the retina respond properly to light.

Strain Development
A 3700 bp EagI fragment of the mouse cyclin D1 gene, (5' to the first coding exon) was used in the targeting vector, and electoporated into D3 ES cells. Cyclin D1 heterozygous ES cells ere injected into C57BL/6 embryos, and the resulting chimeras were bred to B6 mice. The resulting cyclin D1 heterozygotes were bred to produce homozygous mice. The targeting vector deletes coding portions of exons I-III of the cyclin D1 gene and replaces them with the neomycin resistance gene.

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Ccnd1tm1Wbg/Ccnd1tm1Wbg         involves: 129S2/SvPas lethality-postnatal postnatal lethality (MGI Ref ID J:105045) about 25% die during the first month of life growth/size phenotype postnatal growth retardation (MGI Ref ID J:105045) by the third week of life, mutants were approximately 1/2 of the size of wildtype vision/eye phenotype abnormal eye electrophysiology (MGI Ref ID J:95994) reduced amplitudes of the a- and b-waves in retinas in response to a pulse of light, with a-waves corresponding to 15% of those seen in wildtype retinas respond to light with electroretinographic potentials corresponding to about 10% of those seen in wild type retina hypoplasia (MGI Ref ID J:105045) all cell layers (outer nuclear, inner nuclear, and ganglion cell layers) of the retina were hypoplastic endocrine/exocrine gland phenotype abnormal branching of the mammary ductal tree (MGI Ref ID J:95994) underdeveloped mammary epithelial tree at the end of pregnancy abnormal lactation (MGI Ref ID J:95994) unable to breast-feed pups due to abnormal mammary tissue development reproductive system phenotype abnormal branching of the mammary ductal tree (MGI Ref ID J:95994) underdeveloped mammary epithelial tree at the end of pregnancy abnormal lactation (MGI Ref ID J:95994) unable to breast-feed pups due to abnormal mammary tissue development behavior/neurological phenotype limb grasping (MGI Ref ID J:95994) when lifted by tails, responded by rapidly retracting their limbs

Gene & Allele Details

Allele Symbol		Ccnd1tm1Wbg
Allele Name		targeted mutation 1, Robert Weinberg
Common Name(s)		CD1-; cyclin D1-;
Mutation Made By		Robert Weinberg,   Massachusetts Institute of Technology
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Ccnd1, cyclin D1
Chromosome		7
Gene Common Name(s)		AI327039; BCL1; Cyl-1; D11S287E; PRAD1; U21B31; bcl-1; cD1; expressed sequence AI327039;
Molecular Note		A genomic fragment containing part of exon 1, exon 2 and exon 3 was replaced with a neomycin selection cassette. The deleted sequences encode the cyclin box domain thought to be required for protein activity. Western blot analysis on embryonic fibroblasts derived from homozygous mice confirmed that no stable protein was made from this allele. [MGI Ref ID J:28419]

Control Information

	Control
	Wild-type from the colony
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Genotyping Protocols

Ccnd^tm1Wbg

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Related Strains

Strains carrying   Ccnd1^tm1Wbg allele

002935

FVB.129S2(B6)-Ccnd1tm1Wbg/J

View Strains carrying   Ccnd1^tm1Wbg     (1 strain)

Strains carrying other alleles of Ccnd1

005143	C3.B6-Tg(Fabp1-Ccnd1)4Rdb/J
004834	C57BL/6-Tg(Fabp1-Ccnd1)4Rdb/J

View Strains carrying other alleles of Ccnd1     (2 strains)

Research Applications

This mouse can be used to support research in many areas including:

Ccnd1^tm1Wbg related

Cancer Research
Genes Regulating Growth and Proliferation
Other

Cell Biology Research
Cell Cycle Regulation
Genes Regulating Growth and Proliferation

Developmental Biology Research
Eye Defects
Growth Defects
Postnatal Mortality

Sensorineural Research
Eye Defects

References

Selected Reference(s)

Sicinski P; Donaher JL; Parker SB; Li T; Fazeli A; Gardner H; Haslam SZ; Bronson RT; Elledge SJ; Weinberg RA. 1995. Cyclin D1 provides a link between development and oncogenesis in the retina and breast. Cell 82(4):621-30. [PubMed: 7664341]  [MGI Ref ID J:28419]

Additional References

---

Price and Supply Information

Strain Name:	B6;129S2-Ccnd1tm1Wbg/J
Stock Number:	002537

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.

• USA, Canada and Mexico
• International

*NO Shipping Destination selected!

 

Supply Details

Standard Supply	Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes	Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Go to JAX^® Mice Query Form