Strain Name:

FVB.129P2-Abcb4tm1Bor/J

Stock Number:

002539

Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain FVB/N
Donor Strain 129P2 via E14TG2a ES cell line
GenerationN6F10pN1p (09-NOV-05)
 
Donating Investigator Piet Borst,   The Netherlands Cancer Institute

Appearance
albino
Related Genotype: Tyrc/Tyrc

Important Note
This strain is homozygous for the retinal degeneration allele Pde6brd1.

Description
Mice homozygous for the Abcb4tm1Bor mutation lack the ability to secrete phospholipid into the bile from the liver. They develop a degenerative liver disease. Portal inflammation ensues at an early age followed by hepatocellular carcinoma development after the age of 1 year. Bile from heterozygous mice has half the level of phospholipid when compared to bile from homozygotes. No liver pathology has been shown in heterozygotes.

Control Information

  Control
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Pde6brd1 allele
004202   B6.C3 Pde6brd1 Hps4le/+ +-Lmx1adr-8J/J
000002   B6.C3-Pde6brd1 Hps4le/J
001022   B6C3FeF1/J a/a
000652   BDP/J
000653   BUB/BnJ
002439   C3.129P2(B6)-B2mtm1Unc/J
005494   C3.129S1(B6)-Grm1rcw/J
000509   C3.Cg-Lystbg-2J/J
000480   C3.MRL-Faslpr/J
001957   C3A Pde6brd1.O20/A-Prph2Rd2/J
005973   C3Bir.129P2(B6)-Il10C3Bir/LtJ
004326   C3Bir.129P2(B6)-Il10tm1Cgn/Lt
003968   C3Bir.129P2(B6)-Il10tm1Cgn/LtJ
001906   C3Ga.Cg-Catb/J
001904   C3H-Atcayji-hes/J
000659   C3H/HeJ
000784   C3H/HeJ-Faslgld/J
002433   C3H/HeJ-Spnb4qv-lnd2J/J
005972   C3H/HeJBirLtJ
001824   C3H/HeJSxJ
000635   C3H/HeOuJ
000474   C3H/HeSn
001431   C3H/HeSn-ocd/J
000661   C3H/HeSnJ
002235   C3H/HeSnJ-Ctnna2cdf/J
002333   C3H/HeSnJ-gri/J
006435   C3HeB.SW-Soaa/MonJ
000658   C3HeB/FeJ
001576   C3HeB/FeJ-Atp7btx-J/J
002588   C3HeB/FeJ-Eya1bor/J
001533   C3HeB/FeJ-Mc1rE-so Gli3Xt-J/J
001886   C3HeB/FeJLe a/a-gnd/J
001908   C3HfB/BiJ
001502   C3Sn.B6-Epha4rb/EiGrsrJ
001547   C3Sn.Cg-Cm/J
000656   CBA/J
000813   CBA/J-Atp7aMo-pew/J
000660   DA/HuSnJ
000023   FL/1ReJ
000025   FL/4ReJ
003024   FVB.129P2(B6)-Fmr1tm1Cgr/J
002935   FVB.129S2(B6)-Ccnd1tm1Wbg/J
002953   FVB.Cg-Tg(MMTVTGFA)254Rjc/J
003170   FVB.Cg-Tg(Myh6-tTA)6Smbf/J
003078   FVB.Cg-Tg(WapIgf1)39Dlr/J
003257   FVB/N-Tg(GFAPGFP)14Mes/J
002374   FVB/N-Tg(MMTV-PyVT)634Mul/J
002856   FVB/N-Tg(TIE2-lacZ)182Sato/J
002384   FVB/N-Tg(UcpDta)1Kz/J
001800   FVB/NJ
003487   FVB/NJ-Tg(XGFAP-lacZ)3Mes/J
001491   FVB/NMob
000734   MOLD/RkJ
000550   MOLF/EiJ
002423   NON/ShiLtJ
000679   P/J
000680   PL/J
100299   PLSJLF1/J
000269   SB/LeJ
005651   SJL.AK-Thy1a/TseJ
000686   SJL/J
000688   ST/bJ
004808   STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
002648   STOCK a/a Cln6nclf/J
000279   STOCK gr +/+ Ap3d1mh/J
005965   STOCK Tg(Pomc1-cre)16Lowl/J
004770   SW.B6-Soab/J
002023   SWR.M-Emv21 Emv22/J
000689   SWR/J
000939   SWR/J-Clcn1adr-mto/J
000692   WB/ReJ KitW/J
100410   WBB6F1/J-KitW/KitW-v/J
000693   WC/ReJ KitlSl/J
100401   WCB6F1/J KitlSl KitlSl-d
View Strains carrying   Pde6brd1     (74 strains)

View Strains carrying other alleles of Pde6b     (10 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Abcb4tm1Bor/Abcb4tm1Bor

        FVB.129P2-Abcb4tm1Bor/J
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype (MGI Ref ID J:100491)
    • mice exhibit normal plasma levels of sitosterol and campesterol
    • increased circulating alanine transaminase level (MGI Ref ID J:100491)
    • increased circulating aspartate transaminase level (MGI Ref ID J:100491)
  • liver/biliary system phenotype
  • abnormal bile composition (MGI Ref ID J:100491)
    • bile cholesterol levels are decreased compared to in wild-type mice
  • digestive/alimentary phenotype
  • abnormal feces composition (MGI Ref ID J:100491)
    • fecal acidic sterol levels are decreased 30% compared to in wild-type mice
    • however, fecal neutral sterol levels are normal

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Abcb4tm1Bor/Abcb4tm1Bor

        either: (involves: 129P2/OlaHsd) or (involves: 129P2/OlaHsd * FVB/N)
  • homeostasis/metabolism phenotype
  • abnormal blood homeostasis (MGI Ref ID J:15531)
    • serum is yellowish, suggesting liver damage
    • abnormal circulating alanine transaminase level (MGI Ref ID J:15531)
      • 5.8-fold increase in alanine transaminase level
    • abnormal circulating alkaline phosphatase level (MGI Ref ID J:15531)
      • 3-fold increase in serum alkaline phosphatase level
    • abnormal circulating aspartate transaminase level (MGI Ref ID J:15531)
      • 4-fold increase in aspartate transaminase level
    • abnormal circulating bilirubin level (MGI Ref ID J:15531)
      • 12-fold elevated bilirubin level; ~50% of this is conjugated
  • immune system phenotype
  • bile duct inflammation (MGI Ref ID J:15531)
    • inflammation of bile ducts
  • liver inflammation (MGI Ref ID J:15531)
    • portal inflammation with mixed inflammatory infiltrate and slight fibrosis
  • liver/biliary system phenotype
  • abnormal bile canaliculus morphology (MGI Ref ID J:15531)
    • prominent widening and increased tortuosity of bile canaliculi with loss of microvilli is present
  • abnormal bile composition (MGI Ref ID J:15531)
    • absence of phospholipid secretion in bile, lipid secretion is decreased and there is a 15-reduction in cholesterol secretion in bile
    • glutathione secretion in bile is ~14% of wild-type
    • chloride secretion is slightly, but significantly, elevated
    • phospolipid to bile salt ratio is only ~41% of wild-type
  • abnormal bile duct morphology (MGI Ref ID J:15531)
    • portal expansion due to ductular proliferation is seen
  • abnormal bile secretion (MGI Ref ID J:15531)
  • abnormal hepatocyte morphology (MGI Ref ID J:15531)
    • hepatocyte degeneration, irregular size with nuclear polymorphism and focal necrosis are observed, along with increased proliferation
  • bile duct inflammation (MGI Ref ID J:15531)
    • inflammation of bile ducts
  • dilated bile duct (MGI Ref ID J:15531)
    • larger bile ducts are dilated
  • intrahepatic cholestasis (MGI Ref ID J:15531)
    • modesrate liver damage with cholestatic properties (raised serum-conjugated bilirubin) is observed
  • liver inflammation (MGI Ref ID J:15531)
    • portal inflammation with mixed inflammatory infiltrate and slight fibrosis
  • endocrine/exocrine gland phenotype
  • abnormal bile duct morphology (MGI Ref ID J:15531)
    • portal expansion due to ductular proliferation is seen

Abcb4tm1Bor/Abcb4tm1Bor

        involves: 129P2/OlaHsd * FVB
  • homeostasis/metabolism phenotype
  • abnormal circulating enzyme level (MGI Ref ID J:114411)
    • increased circulating alanine transaminase level (MGI Ref ID J:114411)
    • increased circulating aspartate transaminase level (MGI Ref ID J:114411)
  • abnormal lipid homeostasis (MGI Ref ID J:114411)
    • decreased cholesterol absorption (MGI Ref ID J:114411)
      • 40% reduction in absorption efficiency
    • decreased circulating cholesterol level (MGI Ref ID J:114411)
      • plasma levels reduced
  • increased circulating bilirubin level (MGI Ref ID J:114411)
  • digestive/alimentary phenotype
  • decreased cholesterol absorption (MGI Ref ID J:114411)
    • 40% reduction in absorption efficiency
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence
      Hepatomas: hepatacellular carcinoma

Abcb4tm1Bor related

Cancer Research
Toxicology

Internal/Organ Research
Liver Defects

Metabolism Research

Research Tools
Toxicology Research

Pde6brd1 related

Mouse/Human Gene Homologs
retinitis pigmentosa, autosomal recessive

Sensorineural Research
Retinal Degeneration

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Abcb4tm1Bor
Allele Name targeted mutation 1, Piet Borst
Allele Type Targeted (knock-out)
Common Name(s) Pgy2tm1Bor; mdr2-; mdr2neo1;
Mutation Made By Piet Borst,   The Netherlands Cancer Institute
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Abcb4, ATP-binding cassette, sub-family B (MDR/TAP), member 4
Chromosome 5
Gene Common Name(s) ABC21; GBD1; MDR2; MDR2/3; MDR3; P glycoprotein 2; PFIC-3; PGY3; Pgy-2; Pgy2; mdr-2;
Molecular Note A neomycin selection cassette replaced a genomic fragment containing exons 1 and 2. The translation initiation site is in exon 2. RNase proteiction assays indicated that mutant truncated transcripts are produced from this allele that contain exons 3-6 or 4-6. [MGI Ref ID J:15531]
 
Allele Symbol Pde6brd1
Allele Name retinal degeneration 1
Allele Type Spontaneous
Common Name(s) Pdebrd1; rd; rd-1; rd1; rodless retina;

Genotyping

Genotyping Information

Genotyping Protocols

Abcb4tm1Bor, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Smit JJ; Schinkel AH; Oude Elferink RP; Groen AK; Wagenaar E; van Deemter L; Mol CA; Ottenhoff R; van der Lugt NM; van Roon MA; van der Valk MA; Offerhaus GJ; Berns AJ; Borst P. 1993. Homozygous disruption of the murine mdr2 P-glycoprotein gene leads to a complete absence of phospholipid from bile and to liver disease. Cell 75(3):451-62. [PubMed: 8106172]  [MGI Ref ID J:15531]

Additional References

Borst P; Schinkel AH. 1996. What have we learnt thus far from mice with disrupted P-glycoprotein genes? Eur J Cancer 32A(6):985-90. [PubMed: 8763339]  [MGI Ref ID J:33581]

Fickert P; Fuchsbichler A; Wagner M; Zollner G; Kaser A; Tilg H; Krause R; Lammert F; Langner C; Zatloukal K; Marschall HU; Denk H; Trauner M. 2004. Regurgitation of bile acids from leaky bile ducts causes sclerosing cholangitis in Mdr2 (Abcb4) knockout mice. Gastroenterology 127(1):261-74. [PubMed: 15236191]  [MGI Ref ID J:93576]

Abcb4tm1Bor related

Borst P; Schinkel AH. 1996. What have we learnt thus far from mice with disrupted P-glycoprotein genes? Eur J Cancer 32A(6):985-90. [PubMed: 8763339]  [MGI Ref ID J:33581]

De Vree JM; Ottenhoff R; Bosma PJ; Smith AJ; Aten J; Elferink RP. 2000. Correction of liver disease by hepatocyte transplantation in a mouse model of progressive familial intrahepatic cholestasis Gastroenterology 119(6):1720-30. [PubMed: 11113093]  [MGI Ref ID J:66180]

Dekkers DW; Comfurius P; Schroit AJ; Bevers EM; Zwaal RF. 1998. Transbilayer movement of NBD-labeled phospholipids in red blood cell membranes: outward-directed transport by the multidrug resistance protein 1 (MRP1). Biochemistry 37(42):14833-7. [PubMed: 9778357]  [MGI Ref ID J:51135]

Eisenbraun MD; Miller RA. 1999. mdr1a-encoded P-glycoprotein is not required for peripheral T cell proliferation, cytokine release, or cytotoxic effector function in mice. J Immunol 163(5):2621-7. [PubMed: 10453001]  [MGI Ref ID J:57091]

Fickert P; Fuchsbichler A; Wagner M; Zollner G; Kaser A; Tilg H; Krause R; Lammert F; Langner C; Zatloukal K; Marschall HU; Denk H; Trauner M. 2004. Regurgitation of bile acids from leaky bile ducts causes sclerosing cholangitis in Mdr2 (Abcb4) knockout mice. Gastroenterology 127(1):261-74. [PubMed: 15236191]  [MGI Ref ID J:93576]

Frijters CM; Tuijn CJ; Ottenhoff R; Zegers BN; Groen AK; Elferink RP. 1999. The role of different P-glycoproteins in hepatobiliary secretion of fluorescently labeled short-chain phospholipids. J Lipid Res 40(11):1950-8. [PubMed: 10552998]  [MGI Ref ID J:119840]

Groen AK; Van Wijland MJ; Frederiks WM; Smit JJ; Schinkel AH; Oude Elferink RP. 1995. Regulation of protein secretion into bile: studies in mice with a disrupted mdr2 p-glycoprotein gene. Gastroenterology 109(6):1997-2006. [PubMed: 7498666]  [MGI Ref ID J:30489]

Hulzebos CV; Voshol PJ; Wolters H; Kruit JK; Ottenhof R; Groen AK; Stellaard F; Verkade HJ; Kuipers F. 2005. Bile duct proliferation associated with bile salt-induced hypercholeresis in Mdr2 P-glycoprotein-deficient mice. Liver Int 25(3):604-12. [PubMed: 15910498]  [MGI Ref ID J:110130]

Katzenellenbogen M; Mizrahi L; Pappo O; Klopstock N; Olam D; Barash H; Domany E; Galun E; Goldenberg D. 2007. Molecular mechanisms of the chemopreventive effect on hepatocellular carcinoma development in Mdr2 knockout mice. Mol Cancer Ther 6(4):1283-91. [PubMed: 17431106]  [MGI Ref ID J:133445]

Katzenellenbogen M; Mizrahi L; Pappo O; Klopstock N; Olam D; Jacob-Hirsch J; Amariglio N; Rechavi G; Domany E; Galun E; Goldenberg D. 2007. Molecular mechanisms of liver carcinogenesis in the mdr2-knockout mice. Mol Cancer Res 5(11):1159-70. [PubMed: 18025261]  [MGI Ref ID J:131750]

Katzenellenbogen M; Pappo O; Barash H; Klopstock N; Mizrahi L; Olam D; Jacob-Hirsch J; Amariglio N; Rechavi G; Mitchell LA; Kohen R; Domany E; Galun E; Goldenberg D. 2006. Multiple adaptive mechanisms to chronic liver disease revealed at early stages of liver carcinogenesis in the Mdr2-knockout mice. Cancer Res 66(8):4001-10. [PubMed: 16618719]  [MGI Ref ID J:108307]

Klopstock N; Katzenellenbogen M; Pappo O; Sklair-Levy M; Olam D; Mizrahi L; Potikha T; Galun E; Goldenberg D. 2009. HCV tumor promoting effect is dependent on host genetic background. PLoS ONE 4(4):e5025. [PubMed: 19340302]  [MGI Ref ID J:148175]

Kok T; Wolters H; Bloks VW; Havinga R; Jansen PL; Staels B; Kuipers F. 2003. Induction of hepatic ABC transporter expression is part of the PPARalpha-mediated fasting response in the mouse. Gastroenterology 124(1):160-71. [PubMed: 12512040]  [MGI Ref ID J:81018]

Kruit JK; Plosch T; Havinga R; Boverhof R; Groot PH; Groen AK; Kuipers F. 2005. Increased fecal neutral sterol loss upon liver X receptor activation is independent of biliary sterol secretion in mice. Gastroenterology 128(1):147-56. [PubMed: 15633131]  [MGI Ref ID J:95371]

Lammert F; Wang DQ; Hillebrandt S; Geier A; Fickert P; Trauner M; Matern S; Paigen B; Carey MC. 2004. Spontaneous cholecysto- and hepatolithiasis in Mdr2-/- mice: a model for low phospholipid-associated cholelithiasis. Hepatology 39(1):117-28. [PubMed: 14752830]  [MGI Ref ID J:105357]

Langheim S; Yu L; von Bergmann K; Lutjohann D; Xu F; Hobbs HH; Cohen JC. 2005. ABCG5 and ABCG8 require MDR2 for secretion of cholesterol into bile. J Lipid Res 46(8):1732-8. [PubMed: 15930516]  [MGI Ref ID J:100491]

Li Z; Agellon LB; Allen TM; Umeda M; Jewell L; Mason A; Vance DE. 2006. The ratio of phosphatidylcholine to phosphatidylethanolamine influences membrane integrity and steatohepatitis. Cell Metab 3(5):321-31. [PubMed: 16679290]  [MGI Ref ID J:129649]

Li Z; Agellon LB; Vance DE. 2007. Choline redistribution during adaptation to choline deprivation. J Biol Chem 282(14):10283-9. [PubMed: 17283071]  [MGI Ref ID J:121149]

Li Z; Agellon LB; Vance DE. 2005. Phosphatidylcholine homeostasis and liver failure. J Biol Chem 280(45):37798-802. [PubMed: 16144842]  [MGI Ref ID J:102893]

Mann DA; Oakley F. 2005. NF-kappaB: a signal for cancer. J Hepatol 42(4):610-1. [PubMed: 15763352]  [MGI Ref ID J:97853]

Mauad TH; van Nieuwkerk CM; Dingemans KP; Smit JJ; Schinkel AH; Notenboom RG; van den Bergh Weerman MA; Verkruisen RP; Groen AK; Oude Elferink RP; van der Valk MA; Borst P; Offerhaus GJ. 1994. Mice with homozygous disruption of the mdr2 P-glycoprotein gene. A novel animal model for studies of nonsuppurative inflammatory cholangitis and hepatocarcinogenesis. Am J Pathol 145(5):1237-45. [PubMed: 7977654]  [MGI Ref ID J:21232]

Minich DM; Voshol PJ; Havinga R; Stellaard F; Kuipers F; Vonk RJ; Verkade HJ. 1999. Biliary phospholipid secretion is not required for intestinal absorption and plasma status of linoleic acid in mice. Biochim Biophys Acta 1441(1):14-22. [PubMed: 10526224]  [MGI Ref ID J:114159]

Mustacich DJ; Shields J; Horton RA; Brown MK; Reed DJ. 1998. Biliary secretion of alpha-tocopherol and the role of the mdr2 P-glycoprotein in rats and mice. Arch Biochem Biophys 350(2):183-92. [PubMed: 9473291]  [MGI Ref ID J:45890]

Oude Elferink RP; Ottenhoff R; van Wijland M; Smit JJ; Schinkel AH; Groen AK. 1995. Regulation of biliary lipid secretion by mdr2 P-glycoprotein in the mouse. J Clin Invest 95(1):31-8. [PubMed: 7814632]  [MGI Ref ID J:22312]

Patsenker E; Popov Y; Stickel F; Jonczyk A; Goodman SL; Schuppan D. 2008. Inhibition of integrin alphavbeta6 on cholangiocytes blocks transforming growth factor-beta activation and retards biliary fibrosis progression. Gastroenterology 135(2):660-70. [PubMed: 18538673]  [MGI Ref ID J:141794]

Pikarsky E; Porat RM; Stein I; Abramovitch R; Amit S; Kasem S; Gutkovich-Pyest E; Urieli-Shoval S; Galun E; Ben-Neriah Y. 2004. NF-kappaB functions as a tumour promoter in inflammation-associated cancer. Nature 431(7007):461-6. [PubMed: 15329734]  [MGI Ref ID J:92668]

Plosch T; Bloks VW; Baller JF; Havinga R; Verkade HJ; Jansen PL; Kuipers F. 2002. Mdr P-glycoproteins are not essential for biliary excretion of the hydrophobic heme precursor protoporphyrin in a griseofulvin-induced mouse model of erythropoietic protoporphyria. Hepatology 35(2):299-306. [PubMed: 11826402]  [MGI Ref ID J:105815]

Rawles LA; Acuna D; Erickson RP. 2007. The role of multiple drug resistance proteins in fetal and/or placental protection against teratogen-induced orofacial clefting. Mol Reprod Dev 74(11):1483-9. [PubMed: 17440929]  [MGI Ref ID J:128360]

Smith AJ; de Vree JM; Ottenhoff R; Oude Elferink RP; Schinkel AH; Borst P. 1998. Hepatocyte-specific expression of the human MDR3 P-glycoprotein gene restores the biliary phosphatidylcholine excretion absent in Mdr2 (-/-) mice. Hepatology 28(2):530-6. [PubMed: 9696021]  [MGI Ref ID J:50317]

Uhr M; Tontsch A; Namendorf C; Ripke S; Lucae S; Ising M; Dose T; Ebinger M; Rosenhagen M; Kohli M; Kloiber S; Salyakina D; Bettecken T; Specht M; Putz B; Binder EB; Muller-Myhsok B; Holsboer F. 2008. Polymorphisms in the drug transporter gene ABCB1 predict antidepressant treatment response in depression. Neuron 57(2):203-9. [PubMed: 18215618]  [MGI Ref ID J:135234]

Van Nieuwkerk CM; Elferink RP; Groen AK; Ottenhoff R; Tytgat GN; Dingemans KP; Van Den Bergh Weerman MA; Offerhaus GJ. 1996. Effects of Ursodeoxycholate and cholate feeding on liver disease in FVB mice with a disrupted mdr2 P-glycoprotein gene. Gastroenterology 111(1):165-71. [PubMed: 8698195]  [MGI Ref ID J:34646]

Voshol PJ; Havinga R; Wolters H; Ottenhoff R; Princen HM; Oude Elferink RP; Groen AK; Kuipers F. 1998. Reduced plasma cholesterol and increased fecal sterol loss in multidrug resistance gene 2 P-glycoprotein-deficient mice. Gastroenterology 114(5):1024-34. [PubMed: 9558293]  [MGI Ref ID J:47805]

Voshol PJ; Minich DM; Havinga R; Elferink RP; Verkade HJ; Groen AK; Kuipers F. 2000. Postprandial chylomicron formation and fat absorption in multidrug resistance gene 2 P-glycoprotein-deficient mice [see comments] Gastroenterology 118(1):173-82. [PubMed: 10611166]  [MGI Ref ID J:59783]

Voshol PJ; Schwarz M; Rigotti A; Krieger M; Groen AK; Kuipers F. 2001. Down-regulation of intestinal scavenger receptor class B, type I (SR-BI) expression in rodents under conditions of deficient bile delivery to the intestine. Biochem J 356(Pt 2):317-25. [PubMed: 11368757]  [MGI Ref ID J:114411]

Werner A; Havinga R; Perton F; Kuipers F; Verkade HJ. 2006. Lymphatic chylomicron size is inversely related to biliary phospholipid secretion in mice. Am J Physiol Gastrointest Liver Physiol 290(6):G1177-85. [PubMed: 16384875]  [MGI Ref ID J:111087]

Werner A; Minich DM; Havinga R; Bloks V; Van Goor H; Kuipers F; Verkade HJ. 2002. Fat malabsorption in essential fatty acid-deficient mice is not due to impaired bile formation. Am J Physiol Gastrointest Liver Physiol 283(4):G900-8. [PubMed: 12223350]  [MGI Ref ID J:108285]

van der Velde AE; Vrins CL; van den Oever K; Kunne C; Oude Elferink RP; Kuipers F; Groen AK. 2007. Direct intestinal cholesterol secretion contributes significantly to total fecal neutral sterol excretion in mice. Gastroenterology 133(3):967-75. [PubMed: 17854600]  [MGI Ref ID J:128386]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryThe strain is maintained by mating homozygous siblings. Some health problems due to liver degeneration begin to arise at about 8 months of age. Expected coat color from breeding:Albino
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Price (US dollars $)
Cryorecovery Fee $1900.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Price (US dollars $)
Cryorecovery Fee $2470.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Supply Details

Standard SupplyCryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.
Important Note
This strain is homozygous for the retinal degeneration allele Pde6brd1.

Control Information

  Control
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Tel: 1-800-422-6423 or 1-207-288-5845
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Terms of Use


General Terms and Conditions


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phone:207-288-6470
fax:207-288-6655

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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