Strain Name:

C57BL/6-Tg(IghelMD4)4Ccg/J

Stock Number:

002595

Order this mouse

Availability:

Repository- Live

Cells from mice carrying the IghelMD4 transgene recognize hen egg lysozyme and have high serum titers of anti-lysozyme antibodies. This strain may be used to study B-cell selection.

Description

Strain Information

Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemInbred x Hemizygote         (Female x Male)   01-MAR-06
Specieslaboratory mouse
GenerationN?+N37 (21-DEC-12)
Generation Definitions
 
Donating InvestigatorDr. Christopher Goodnow,   Stanford University School of Medicine

Appearance
black
Related Genotype: a/a

Description
Cells from mice carrying the IghelMD4 transgene recognize hen egg lysozyme. More than 90% of B-cells in the spleen are derived from the transgene (a allotype) and are predominantly IgM and IgD. This strain may be used to study B-cell selection.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Tg(IghelMD4)4Ccg allele
006345   NOD.B6-Tg(IghelMD4)4Ccg/DvsJ
006608   NOD.Cg-Ighmtm1Cgn Tg(IghelMD4)4Ccg/DvsJ
View Strains carrying   Tg(IghelMD4)4Ccg     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(IghelMD4)4Ccg/0

        involves: C57BL/6
  • immune system phenotype
  • abnormal B cell activation
    • B cells become activated when cultured with HEL protein and CD4 T cells from Tg(TcrHEL3A9)1Mmd transgenic mice   (MGI Ref ID J:73608)
    • B cells in this culture quickly upregulate CD44 and CD86   (MGI Ref ID J:73608)
    • by 48 hours in culture, B cells are greatly enlarged as they undergo blastogenesis   (MGI Ref ID J:73608)
    • B cells cultured without the T cells initially upregulate activation markers but then die   (MGI Ref ID J:73608)
    • when HEL peptide is used in culture, some B cells increase expression of activation markers but do not form large blast cells   (MGI Ref ID J:73608)
  • decreased pre-B cell number
    • bone marrow contains fewer combined numbers of combined pro-pre B cells than on a B6 background   (MGI Ref ID J:115059)
  • decreased pro-B cell number
    • bone marrow contains fewer combined numbers of combined pro-pre B cells than on a B6 background   (MGI Ref ID J:115059)
  • hematopoietic system phenotype
  • abnormal B cell activation
    • B cells become activated when cultured with HEL protein and CD4 T cells from Tg(TcrHEL3A9)1Mmd transgenic mice   (MGI Ref ID J:73608)
    • B cells in this culture quickly upregulate CD44 and CD86   (MGI Ref ID J:73608)
    • by 48 hours in culture, B cells are greatly enlarged as they undergo blastogenesis   (MGI Ref ID J:73608)
    • B cells cultured without the T cells initially upregulate activation markers but then die   (MGI Ref ID J:73608)
    • when HEL peptide is used in culture, some B cells increase expression of activation markers but do not form large blast cells   (MGI Ref ID J:73608)
  • decreased pre-B cell number
    • bone marrow contains fewer combined numbers of combined pro-pre B cells than on a B6 background   (MGI Ref ID J:115059)
  • decreased pro-B cell number
    • bone marrow contains fewer combined numbers of combined pro-pre B cells than on a B6 background   (MGI Ref ID J:115059)

Tg(IghelMD4)4Ccg/0

        C57BL/6-Tg(IghelMD4)4Ccg
  • immune system phenotype
  • abnormal B cell morphology   (MGI Ref ID J:78308)
    • over 90% of B cells express immunoglobulin that has a high affinity for hen egg lysozyme antigen (HEL)   (MGI Ref ID J:132538)
    • most peripheral B cells express the transgenic immunoglobulin (Ig) that binds hen egg lysozyme peptide (HEL) with a high affinity   (MGI Ref ID J:109923)
    • the few B cells expressing endogenous Ig in the spleen express high levels of IgM with little to no levels of IgD compared to splenic B cells from wild-type mice that are IgMlow IgDhigh   (MGI Ref ID J:109923)
    • abnormal immature B cell morphology
      • there are reduced numbers of immature B cells bearing transgenic IgM in the bone marrow   (MGI Ref ID J:109923)
      • increased immature B cell number
        • three quarters of the B cells in the spleen are immature as indicated by the IgMhi IgDhi surface markers   (MGI Ref ID J:132538)
        • three quarters of the B cells in the spleen are immature as indicated by the IgMhi IgDhi surface markers   (MGI Ref ID J:78308)
    • abnormal mature B cell morphology
      • 98% of the B cells express the transgenic antibody that is specific for hen egg lysozyme (HEL)   (MGI Ref ID J:89156)
      • 94% of these B cells retain the ability to bind HEL protein   (MGI Ref ID J:89156)
      • there is an increased percentage of mature B cells in the bone marrow   (MGI Ref ID J:109923)
      • abnormal B-2 B cell morphology
        • all of the B cells found in the peritoneal cavity are of the B-2 lineage as determined by B220hi CD5lowexpression   (MGI Ref ID J:132538)
      • abnormal germinal center B cell morphology
        • B cells expressing the transgenic Ig are absent from the germinal center of the spleen and lymph nodes   (MGI Ref ID J:109923)
    • abnormal plasma cell morphology
      • plasma cells bearing endogenous Ig outnumber plasma cells bearing the transgenic Ig   (MGI Ref ID J:109923)
    • abnormal pro-B cell morphology
      • there are reduced numbers of pro-B cells bearing transgenic IgM in the bone marrow   (MGI Ref ID J:109923)
  • increased IgG2b level
    • there are low levels of anti-HEL IgG2b in the sera of unimmunized mice   (MGI Ref ID J:132538)
  • increased IgM level
    • there are high levels of anti-HEL IgM in the sera of unimmunized mice   (MGI Ref ID J:132538)
    • there are high levels of anti-HEL IgM in the sera of unimmunized mice   (MGI Ref ID J:78308)
  • hematopoietic system phenotype
  • abnormal B cell morphology   (MGI Ref ID J:78308)
    • over 90% of B cells express immunoglobulin that has a high affinity for hen egg lysozyme antigen (HEL)   (MGI Ref ID J:132538)
    • most peripheral B cells express the transgenic immunoglobulin (Ig) that binds hen egg lysozyme peptide (HEL) with a high affinity   (MGI Ref ID J:109923)
    • the few B cells expressing endogenous Ig in the spleen express high levels of IgM with little to no levels of IgD compared to splenic B cells from wild-type mice that are IgMlow IgDhigh   (MGI Ref ID J:109923)
    • abnormal immature B cell morphology
      • there are reduced numbers of immature B cells bearing transgenic IgM in the bone marrow   (MGI Ref ID J:109923)
      • increased immature B cell number
        • three quarters of the B cells in the spleen are immature as indicated by the IgMhi IgDhi surface markers   (MGI Ref ID J:132538)
        • three quarters of the B cells in the spleen are immature as indicated by the IgMhi IgDhi surface markers   (MGI Ref ID J:78308)
    • abnormal mature B cell morphology
      • 98% of the B cells express the transgenic antibody that is specific for hen egg lysozyme (HEL)   (MGI Ref ID J:89156)
      • 94% of these B cells retain the ability to bind HEL protein   (MGI Ref ID J:89156)
      • there is an increased percentage of mature B cells in the bone marrow   (MGI Ref ID J:109923)
      • abnormal B-2 B cell morphology
        • all of the B cells found in the peritoneal cavity are of the B-2 lineage as determined by B220hi CD5lowexpression   (MGI Ref ID J:132538)
      • abnormal germinal center B cell morphology
        • B cells expressing the transgenic Ig are absent from the germinal center of the spleen and lymph nodes   (MGI Ref ID J:109923)
    • abnormal plasma cell morphology
      • plasma cells bearing endogenous Ig outnumber plasma cells bearing the transgenic Ig   (MGI Ref ID J:109923)
    • abnormal pro-B cell morphology
      • there are reduced numbers of pro-B cells bearing transgenic IgM in the bone marrow   (MGI Ref ID J:109923)
  • increased IgG2b level
    • there are low levels of anti-HEL IgG2b in the sera of unimmunized mice   (MGI Ref ID J:132538)
  • increased IgM level
    • there are high levels of anti-HEL IgM in the sera of unimmunized mice   (MGI Ref ID J:132538)
    • there are high levels of anti-HEL IgM in the sera of unimmunized mice   (MGI Ref ID J:78308)

Tg(IghelMD4)4Ccg/Tg(IghelMD4)4Ccg

        involves: C57BL/6
  • immune system phenotype
  • decreased pre-B cell number   (MGI Ref ID J:171924)
  • decreased pro-B cell number   (MGI Ref ID J:171924)
  • hematopoietic system phenotype
  • decreased pre-B cell number   (MGI Ref ID J:171924)
  • decreased pro-B cell number   (MGI Ref ID J:171924)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Tg(IghelMD4)4Ccg/0

        involves: C3H/HeJ * C57BL/6
  • hematopoietic system phenotype
  • decreased pre-B cell number
    • bone marrow contains fewer combined numbers of combined pro-pre B cells than on a B6 background   (MGI Ref ID J:115059)
  • decreased pro-B cell number
    • bone marrow contains fewer combined numbers of combined pro-pre B cells than on a B6 background   (MGI Ref ID J:115059)
  • immune system phenotype
  • decreased pre-B cell number
    • bone marrow contains fewer combined numbers of combined pro-pre B cells than on a B6 background   (MGI Ref ID J:115059)
  • decreased pro-B cell number
    • bone marrow contains fewer combined numbers of combined pro-pre B cells than on a B6 background   (MGI Ref ID J:115059)

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(IghelMD4)4Ccg
Allele Name transgene insertion 4, Christopher C Goodnow
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) HELmuMT; Hel-Ig; Ig-transgenic; IgHEL; IgTg; MD-3; MD4; MD4 HEL Ig; MD4 Ig; MD4 Ig BCR Tg; MD4-RAG; SWHEL;
Mutation Made ByDr. Christopher Goodnow,   Stanford University School of Medicine
Strain of OriginC57BL/6
Expressed Gene Ighg, Immunoglobulin heavy chain (gamma polypeptide), mouse, laboratory
Promoter Ighg, Immunoglobulin heavy chain (gamma polypeptide), mouse, laboratory
Molecular Note The hybridoma HyHEL10 produces an antibody with high affinity for hen egg lysozyme. Genomic clones encoding HyHEL10 immunoglobulin heavy and light chains were used for this construct. The gamma1-constant region of the heavy chain was replaced by a segment containing the mu and delta constant region genes derived from BALB/c mice. The light and heavy chains were co-injected into C57BL/6 eggs. Five lines, MD3-MD7, that carried multiple copies of the transgene construct were created. The MD4 line had thehighest expression of the transgene and was propogated for further studies. Mice carrying this transgene had high serum titers of anti-lysozyme antibodies. [MGI Ref ID J:78308]
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(IghelMD4)4Ccg, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Mason DY; Jones M; Goodnow CC. 1992. Development and follicular localization of tolerant B lymphocytes in lysozyme/anti-lysozyme IgM/IgD transgenic mice. Int Immunol 4(2):163-75. [PubMed: 1622894]  [MGI Ref ID J:109923]

Additional References

Gerth AJ; Lin L; Neurath MF; Glimcher LH; Peng SL. 2004. An innate cell-mediated, murine ulcerative colitis-like syndrome in the absence of nuclear factor of activated T cells. Gastroenterology 126(4):1115-21. [PubMed: 15057750]  [MGI Ref ID J:93475]

Goodnow CC; Crosbie J; Adelstein S; Lavoie TB; Smith-Gill SJ; Brink RA; Pritchard-Briscoe H; Wotherspoon JS; Loblay RH; Raphael K; et al.. 1988. Altered immunoglobulin expression and functional silencing of self-reactive B lymphocytes in transgenic mice. Nature 334(6184):676-82. [PubMed: 3261841]  [MGI Ref ID J:78308]

Tg(IghelMD4)4Ccg related

Akkaraju S; Ho WY; Leong D; Canaan K; Davis MM; Goodnow CC. 1997. A range of CD4 T cell tolerance: partial inactivation to organ-specific antigen allows nondestructive thyroiditis or insulitis. Immunity 7(2):255-71. [PubMed: 9285410]  [MGI Ref ID J:78309]

Albacker LA; Karisola P; Chang YJ; Umetsu SE; Zhou M; Akbari O; Kobayashi N; Baumgarth N; Freeman GJ; Umetsu DT; Dekruyff RH. 2010. TIM-4, a Receptor for Phosphatidylserine, Controls Adaptive Immunity by Regulating the Removal of Antigen-Specific T Cells. J Immunol 185(11):6839-49. [PubMed: 21037090]  [MGI Ref ID J:166143]

Arana E; Vehlow A; Harwood NE; Vigorito E; Henderson R; Turner M; Tybulewicz VL; Batista FD. 2008. Activation of the small GTPase Rac2 via the B cell receptor regulates B cell adhesion and immunological-synapse formation. Immunity 28(1):88-99. [PubMed: 18191593]  [MGI Ref ID J:131150]

Ashour HM; Niederkorn JY. 2006. Peripheral tolerance via the anterior chamber of the eye: role of B cells in MHC class I and II antigen presentation. J Immunol 176(10):5950-7. [PubMed: 16670303]  [MGI Ref ID J:131707]

Baba M; Keller JR; Sun HW; Resch W; Kuchen S; Suh HC; Hasumi H; Hasumi Y; Kieffer-Kwon KR; Gonzalez CG; Hughes RM; Klein ME; Oh HF; Bible P; Southon E; Tessarollo L; Schmidt LS; Linehan WM; Casellas R. 2012. The folliculin-FNIP1 pathway deleted in human Birt-Hogg-Dube syndrome is required for murine B-cell development. Blood 120(6):1254-61. [PubMed: 22709692]  [MGI Ref ID J:189078]

Bai L; Chen Y; He Y; Dai X; Lin X; Wen R; Wang D. 2007. Phospholipase Cgamma2 contributes to light-chain gene activation and receptor editing. Mol Cell Biol 27(17):5957-67. [PubMed: 17591700]  [MGI Ref ID J:125007]

Bajenoff M; Germain RN. 2009. B-cell follicle development remodels the conduit system and allows soluble antigen delivery to follicular dendritic cells. Blood 114(24):4989-97. [PubMed: 19713459]  [MGI Ref ID J:155014]

Barr TA; Brown S; Mastroeni P; Gray D. 2010. TLR and B cell receptor signals to B cells differentially program primary and memory Th1 responses to Salmonella enterica. J Immunol 185(5):2783-9. [PubMed: 20675594]  [MGI Ref ID J:163256]

Barrington RA; Borde M; Rao A; Carroll MC. 2006. Involvement of NFAT1 in B cell self-tolerance. J Immunol 177(3):1510-5. [PubMed: 16849457]  [MGI Ref ID J:137979]

Barrington RA; Schneider TJ; Pitcher LA; Mempel TR; Ma M; Barteneva NS; Carroll MC. 2009. Uncoupling CD21 and CD19 of the B-cell coreceptor. Proc Natl Acad Sci U S A 106(34):14490-5. [PubMed: 19706534]  [MGI Ref ID J:151991]

Barrington RA; Zhang M; Zhong X; Jonsson H; Holodick N; Cherukuri A; Pierce SK; Rothstein TL; Carroll MC. 2005. CD21/CD19 coreceptor signaling promotes B cell survival during primary immune responses. J Immunol 175(5):2859-67. [PubMed: 16116172]  [MGI Ref ID J:113242]

Bemark M; Sale JE; Kim HJ; Berek C; Cosgrove RA; Neuberger MS. 2000. Somatic hypermutation in the absence of DNA-dependent protein kinase catalytic subunit (DNA-PK(cs)) or recombination-activating gene (RAG)1 activity J Exp Med 192(10):1509-14. [PubMed: 11085752]  [MGI Ref ID J:65886]

Blery M; Tze L; Miosge LA; Jun JE; Goodnow CC. 2006. Essential role of membrane cholesterol in accelerated BCR internalization and uncoupling from NF-kappa B in B cell clonal anergy. J Exp Med 203(7):1773-83. [PubMed: 16801401]  [MGI Ref ID J:124402]

Bortnick A; Chernova I; Quinn WJ 3rd; Mugnier M; Cancro MP; Allman D. 2012. Long-lived bone marrow plasma cells are induced early in response to T cell-independent or T cell-dependent antigens. J Immunol 188(11):5389-96. [PubMed: 22529295]  [MGI Ref ID J:188747]

Boulianne B; Rojas OL; Haddad D; Zaheen A; Kapelnikov A; Nguyen T; Li C; Hakem R; Gommerman JL; Martin A. 2013. AID and caspase 8 shape the germinal center response through apoptosis. J Immunol 191(12):5840-7. [PubMed: 24244021]  [MGI Ref ID J:207109]

Bour-Jordan H; Salomon BL; Thompson HL; Santos R; Abbas AK; Bluestone JA. 2007. Constitutive expression of B7-1 on B cells uncovers autoimmunity toward the B cell compartment in the nonobese diabetic mouse. J Immunol 179(2):1004-12. [PubMed: 17617592]  [MGI Ref ID J:131077]

Browne CD; Del Nagro CJ; Cato MH; Dengler HS; Rickert RC. 2009. Suppression of phosphatidylinositol 3,4,5-trisphosphate production is a key determinant of B cell anergy. Immunity 31(5):749-60. [PubMed: 19896393]  [MGI Ref ID J:155314]

Callen E; Jankovic M; Difilippantonio S; Daniel JA; Chen HT; Celeste A; Pellegrini M; McBride K; Wangsa D; Bredemeyer AL; Sleckman BP; Ried T; Nussenzweig M; Nussenzweig A. 2007. ATM prevents the persistence and propagation of chromosome breaks in lymphocytes. Cell 130(1):63-75. [PubMed: 17599403]  [MGI Ref ID J:143821]

Carrasco YR; Batista FD. 2006. B-cell activation by membrane-bound antigens is facilitated by the interaction of VLA-4 with VCAM-1. EMBO J 25(4):889-99. [PubMed: 16456548]  [MGI Ref ID J:106020]

Chackerian B; Durfee MR; Schiller JT. 2008. Virus-like display of a neo-self antigen reverses B cell anergy in a B cell receptor transgenic mouse model. J Immunol 180(9):5816-25. [PubMed: 18424700]  [MGI Ref ID J:134321]

Chan TD; Wood K; Hermes JR; Butt D; Jolly CJ; Basten A; Brink R. 2012. Elimination of germinal-center-derived self-reactive B cells is governed by the location and concentration of self-antigen. Immunity 37(5):893-904. [PubMed: 23142780]  [MGI Ref ID J:190871]

Chang NH; Cheung YH; Loh C; Pau E; Roy V; Cai YC; Wither J. 2010. B cell activating factor (BAFF) and T cells cooperate to breach B cell tolerance in lupus-prone New Zealand Black (NZB) mice. PLoS One 5(7):e11691. [PubMed: 20661465]  [MGI Ref ID J:163088]

Cinamon G; Matloubian M; Lesneski MJ; Xu Y; Low C; Lu T; Proia RL; Cyster JG. 2004. Sphingosine 1-phosphate receptor 1 promotes B cell localization in the splenic marginal zone. Nat Immunol 5(7):713-20. [PubMed: 15184895]  [MGI Ref ID J:91161]

Coffey F; Alabyev B; Manser T. 2009. Initial clonal expansion of germinal center B cells takes place at the perimeter of follicles. Immunity 30(4):599-609. [PubMed: 19303334]  [MGI Ref ID J:147988]

Colonna L; Catalano G; Chew C; D'Agati V; Thomas JW; Wong FS; Schmitz J; Masuda ES; Reizis B; Tarakhovsky A; Clynes R. 2010. Therapeutic targeting of Syk in autoimmune diabetes. J Immunol 185(3):1532-43. [PubMed: 20601600]  [MGI Ref ID J:162249]

Cook MC; Basten A; Fazekas de St Groth B. 1997. Outer periarteriolar lymphoid sheath arrest and subsequent differentiation of both naive and tolerant immunoglobulin transgenic B cells is determined by B cell receptor occupancy. J Exp Med 186(5):631-43. [PubMed: 9271579]  [MGI Ref ID J:132748]

Cornall RJ; Cheng AM; Pawson T; Goodnow CC. 2000. Role of Syk in B-cell development and antigen-receptor signaling. Proc Natl Acad Sci U S A 97(4):1713-8. [PubMed: 10677523]  [MGI Ref ID J:60647]

Cornall RJ; Cyster JG; Hibbs ML; Dunn AR; Otipoby KL; Clark EA; Goodnow CC. 1998. Polygenic autoimmune traits: Lyn, CD22, and SHP-1 are limiting elements of a biochemical pathway regulating BCR signaling and selection. Immunity 8(4):497-508. [PubMed: 9586639]  [MGI Ref ID J:110531]

Cox SL; Stolp J; Hallahan NL; Counotte J; Zhang W; Serreze DV; Basten A; Silveira PA. 2010. Enhanced responsiveness to T-cell help causes loss of B-lymphocyte tolerance to a beta-cell neo-self-antigen in type 1 diabetes prone NOD mice. Eur J Immunol 40(12):3413-25. [PubMed: 21108464]  [MGI Ref ID J:174579]

Deenick EK; Chan A; Ma CS; Gatto D; Schwartzberg PL; Brink R; Tangye SG. 2010. Follicular helper T cell differentiation requires continuous antigen presentation that is independent of unique B cell signaling. Immunity 33(2):241-53. [PubMed: 20691615]  [MGI Ref ID J:163920]

Deng J; Dekruyff RH; Freeman GJ; Umetsu DT; Levy S. 2002. Critical role of CD81 in cognate T-B cell interactions leading to Th2 responses. Int Immunol 14(5):513-23. [PubMed: 11978781]  [MGI Ref ID J:113522]

Depoil D; Fleire S; Treanor BL; Weber M; Harwood NE; Marchbank KL; Tybulewicz VL; Batista FD. 2008. CD19 is essential for B cell activation by promoting B cell receptor-antigen microcluster formation in response to membrane-bound ligand. Nat Immunol 9(1):63-72. [PubMed: 18059271]  [MGI Ref ID J:130478]

Ekland EH; Forster R; Lipp M; Cyster JG. 2004. Requirements for follicular exclusion and competitive elimination of autoantigen-binding B cells. J Immunol 172(8):4700-8. [PubMed: 15067045]  [MGI Ref ID J:89130]

Enders A; Bouillet P; Puthalakath H; Xu Y; Tarlinton DM; Strasser A. 2003. Loss of the pro-apoptotic BH3-only Bcl-2 family member Bim inhibits BCR stimulation-induced apoptosis and deletion of autoreactive B cells. J Exp Med 198(7):1119-26. [PubMed: 14517273]  [MGI Ref ID J:86002]

Ferry H; Crockford TL; Silver K; Rust N; Goodnow CC; Cornall RJ. 2005. Analysis of Lyn/CD22 double-deficient B cells in vivo demonstrates Lyn- and CD22-independent pathways affecting BCR regulation and B cell survival. Eur J Immunol 35(12):3655-63. [PubMed: 16278813]  [MGI Ref ID J:113739]

Fillatreau S; Gray D. 2003. T cell accumulation in B cell follicles is regulated by dendritic cells and is independent of B cell activation. J Exp Med 197(2):195-206. [PubMed: 12538659]  [MGI Ref ID J:124822]

Foote LC; Evans JW; Cifuni JM; Siracusa MC; Monteforte GM; McCole JL; D'Orazio CC; Hastings WD; Rothstein TL. 2004. Interleukin-4 produces a breakdown of tolerance in vivo with autoantibody formation and tissue damage. Autoimmunity 37(8):569-77. [PubMed: 15763919]  [MGI Ref ID J:128253]

Gatto D; Wood K; Caminschi I; Murphy-Durland D; Schofield P; Christ D; Karupiah G; Brink R. 2013. The chemotactic receptor EBI2 regulates the homeostasis, localization and immunological function of splenic dendritic cells. Nat Immunol 14(5):446-53. [PubMed: 23502855]  [MGI Ref ID J:196453]

Gerth AJ; Lin L; Neurath MF; Glimcher LH; Peng SL. 2004. An innate cell-mediated, murine ulcerative colitis-like syndrome in the absence of nuclear factor of activated T cells. Gastroenterology 126(4):1115-21. [PubMed: 15057750]  [MGI Ref ID J:93475]

Getahun A; Smith MJ; Kogut I; van Dyk LF; Cambier JC. 2012. Retention of anergy and inhibition of antibody responses during acute gamma herpesvirus 68 infection. J Immunol 189(6):2965-74. [PubMed: 22904300]  [MGI Ref ID J:189918]

Giltiay NV; Lu Y; Allman D; Jorgensen TN; Li X. 2010. The adaptor molecule Act1 regulates BAFF responsiveness and self-reactive B cell selection during transitional B cell maturation. J Immunol 185(1):99-109. [PubMed: 20543113]  [MGI Ref ID J:161592]

Goodnow CC; Crosbie J; Adelstein S; Lavoie TB; Smith-Gill SJ; Brink RA; Pritchard-Briscoe H; Wotherspoon JS; Loblay RH; Raphael K; et al.. 1988. Altered immunoglobulin expression and functional silencing of self-reactive B lymphocytes in transgenic mice. Nature 334(6184):676-82. [PubMed: 3261841]  [MGI Ref ID J:78308]

Green JA; Suzuki K; Cho B; Willison LD; Palmer D; Allen CD; Schmidt TH; Xu Y; Proia RL; Coughlin SR; Cyster JG. 2011. The sphingosine 1-phosphate receptor S1P maintains the homeostasis of germinal center B cells and promotes niche confinement. Nat Immunol 12(7):672-80. [PubMed: 21642988]  [MGI Ref ID J:174313]

Grewal PK; Boton M; Ramirez K; Collins BE; Saito A; Green RS; Ohtsubo K; Chui D; Marth JD. 2006. ST6Gal-I restrains CD22-dependent antigen receptor endocytosis and Shp-1 recruitment in normal and pathogenic immune signaling. Mol Cell Biol 26(13):4970-81. [PubMed: 16782884]  [MGI Ref ID J:110325]

Grigorova IL; Panteleev M; Cyster JG. 2010. Lymph node cortical sinus organization and relationship to lymphocyte egress dynamics and antigen exposure. Proc Natl Acad Sci U S A 107(47):20447-52. [PubMed: 21059923]  [MGI Ref ID J:166592]

Gross AJ; Lyandres JR; Panigrahi AK; Prak ET; DeFranco AL. 2009. Developmental acquisition of the Lyn-CD22-SHP-1 inhibitory pathway promotes B cell tolerance. J Immunol 182(9):5382-92. [PubMed: 19380785]  [MGI Ref ID J:150309]

Guo B; Rothstein TL. 2013. IL-4 upregulates Igalpha and Igbeta protein, resulting in augmented IgM maturation and B cell receptor-triggered B cell activation. J Immunol 191(2):670-7. [PubMed: 23776171]  [MGI Ref ID J:205452]

Hagn M; Belz GT; Kallies A; Sutton VR; Thia KY; Tarlinton DM; Hawkins ED; Trapani JA. 2012. Activated mouse B cells lack expression of granzyme B. J Immunol 188(8):3886-92. [PubMed: 22427643]  [MGI Ref ID J:184058]

Harris DP; Goodrich S; Gerth AJ; Peng SL; Lund FE. 2005. Regulation of IFN-gamma production by B effector 1 cells: essential roles for T-bet and the IFN-gamma receptor. J Immunol 174(11):6781-90. [PubMed: 15905519]  [MGI Ref ID J:99038]

Harris DP; Goodrich S; Mohrs K; Mohrs M; Lund FE. 2005. Cutting edge: the development of IL-4-producing B cells (B effector 2 cells) is controlled by IL-4, IL-4 receptor alpha, and Th2 cells. J Immunol 175(11):7103-7. [PubMed: 16301612]  [MGI Ref ID J:122200]

Hartley SB; Crosbie J; Brink R; Kantor AB; Basten A; Goodnow CC. 1991. Elimination from peripheral lymphoid tissues of self-reactive B lymphocytes recognizing membrane-bound antigens. Nature 353(6346):765-9. [PubMed: 1944535]  [MGI Ref ID J:78307]

Hartley SB; Goodnow CC. 1994. Censoring of self-reactive B cells with a range of receptor affinities in transgenic mice expressing heavy chains for a lysozyme-specific antibody. Int Immunol 6(9):1417-25. [PubMed: 7819151]  [MGI Ref ID J:132751]

Hermiston ML; Tan AL; Gupta VA; Majeti R; Weiss A. 2005. The juxtamembrane wedge negatively regulates CD45 function in B cells. Immunity 23(6):635-47. [PubMed: 16356861]  [MGI Ref ID J:113310]

Hippen KL; Schram BR; Tze LE; Pape KA; Jenkins MK; Behrens TW. 2005. In vivo assessment of the relative contributions of deletion, anergy, and editing to B cell self-tolerance. J Immunol 175(2):909-16. [PubMed: 16002689]  [MGI Ref ID J:100671]

Hippen KL; Tze LE; Behrens TW. 2000. CD5 maintains tolerance in anergic B cells. J Exp Med 191(5):883-90. [PubMed: 10704468]  [MGI Ref ID J:124695]

Ho WY; Cooke MP; Goodnow CC; Davis MM. 1994. Resting and anergic B cells are defective in CD28-dependent costimulation of naive CD4+ T cells. J Exp Med 179(5):1539-49. [PubMed: 7909325]  [MGI Ref ID J:73608]

Hu CC; Dougan SK; McGehee AM; Love JC; Ploegh HL. 2009. XBP-1 regulates signal transduction, transcription factors and bone marrow colonization in B cells. EMBO J 28(11):1624-36. [PubMed: 19407814]  [MGI Ref ID J:150026]

Hu CC; Dougan SK; Winter SV; Paton AW; Paton JC; Ploegh HL. 2009. Subtilase cytotoxin cleaves newly synthesized BiP and blocks antibody secretion in B lymphocytes. J Exp Med 206(11):2429-40. [PubMed: 19808260]  [MGI Ref ID J:154174]

Hwang IY; Park C; Harrison K; Kehrl JH. 2009. TLR4 signaling augments B lymphocyte migration and overcomes the restriction that limits access to germinal center dark zones. J Exp Med 206(12):2641-57. [PubMed: 19917774]  [MGI Ref ID J:155452]

Jeelall YS; Wang JQ; Law HD; Domaschenz H; Fung HK; Kallies A; Nutt SL; Goodnow CC; Horikawa K. 2012. Human lymphoma mutations reveal CARD11 as the switch between self-antigen-induced B cell death or proliferation and autoantibody production. J Exp Med 209(11):1907-17. [PubMed: 23027925]  [MGI Ref ID J:190942]

Jurado S; Gleeson K; O'Donnell K; Izon DJ; Walkley CR; Strasser A; Tarlinton DM; Heierhorst J. 2012. The Zinc-finger protein ASCIZ regulates B cell development via DYNLL1 and Bim. J Exp Med 209(9):1629-39. [PubMed: 22891272]  [MGI Ref ID J:191832]

Kassmeier MD; Mondal K; Palmer VL; Raval P; Kumar S; Perry GA; Anderson DK; Ciborowski P; Jackson S; Xiong Y; Swanson PC. 2012. VprBP binds full-length RAG1 and is required for B-cell development and V(D)J recombination fidelity. EMBO J 31(4):945-58. [PubMed: 22157821]  [MGI Ref ID J:181758]

Kelly LM; Pereira JP; Yi T; Xu Y; Cyster JG. 2011. EBI2 guides serial movements of activated B cells and ligand activity is detectable in lymphoid and nonlymphoid tissues. J Immunol 187(6):3026-32. [PubMed: 21844396]  [MGI Ref ID J:179238]

Kilmon MA; Rutan JA; Clarke SH; Vilen BJ. 2005. Low-affinity, Smith antigen-specific B cells are tolerized by dendritic cells and macrophages. J Immunol 175(1):37-41. [PubMed: 15972629]  [MGI Ref ID J:100598]

Kilmon MA; Wagner NJ; Garland AL; Lin L; Aviszus K; Wysocki LJ; Vilen BJ. 2007. Macrophages prevent the differentiation of autoreactive B cells by secreting CD40 ligand and interleukin-6. Blood 110(5):1595-602. [PubMed: 17712049]  [MGI Ref ID J:145511]

Kitano M; Moriyama S; Ando Y; Hikida M; Mori Y; Kurosaki T; Okada T. 2011. Bcl6 protein expression shapes pre-germinal center B cell dynamics and follicular helper T cell heterogeneity. Immunity 34(6):961-72. [PubMed: 21636294]  [MGI Ref ID J:174014]

Kitaura Y; Jang IK; Wang Y; Han YC; Inazu T; Cadera EJ; Schlissel M; Hardy RR; Gu H. 2007. Control of the B cell-intrinsic tolerance programs by ubiquitin ligases Cbl and Cbl-b. Immunity 26(5):567-78. [PubMed: 17493844]  [MGI Ref ID J:123596]

Koralov SB; Muljo SA; Galler GR; Krek A; Chakraborty T; Kanellopoulou C; Jensen K; Cobb BS; Merkenschlager M; Rajewsky N; Rajewsky K. 2008. Dicer ablation affects antibody diversity and cell survival in the B lymphocyte lineage. Cell 132(5):860-74. [PubMed: 18329371]  [MGI Ref ID J:135783]

Kretschmer B; Luthje K; Guse AH; Ehrlich S; Koch-Nolte F; Haag F; Fleischer B; Breloer M. 2007. CD83 modulates B Cell function in vitro: increased IL-10 and reduced Ig secretion by CD83Tg B cells. PLoS ONE 2(1):e755. [PubMed: 17710154]  [MGI Ref ID J:129391]

Kumar KR; Li L; Yan M; Bhaskarabhatla M; Mobley AB; Nguyen C; Mooney JM; Schatzle JD; Wakeland EK; Mohan C. 2006. Regulation of B cell tolerance by the lupus susceptibility gene Ly108. Science 312(5780):1665-9. [PubMed: 16778059]  [MGI Ref ID J:109640]

Lee SR; Rutan JA; Monteith AJ; Jones SZ; Kang SA; Krum KN; Kilmon MA; Roques JR; Wagner NJ; Clarke SH; Vilen BJ. 2012. Receptor cross-talk spatially restricts p-ERK during TLR4 stimulation of autoreactive B cells. J Immunol 189(8):3859-68. [PubMed: 22984080]  [MGI Ref ID J:190650]

Li DH; Tung JW; Tarner IH; Snow AL; Yukinari T; Ngernmaneepothong R; Martinez OM; Parnes JR. 2006. CD72 down-modulates BCR-induced signal transduction and diminishes survival in primary mature B lymphocytes. J Immunol 176(9):5321-8. [PubMed: 16621999]  [MGI Ref ID J:131658]

Liu C; Bai X; Wu J; Sharma S; Upadhyaya A; Dahlberg CI; Westerberg LS; Snapper SB; Zhao X; Song W. 2013. N-wasp is essential for the negative regulation of B cell receptor signaling. PLoS Biol 11(11):e1001704. [PubMed: 24223520]  [MGI Ref ID J:205106]

Liu X; Wysocki LJ; Manser T. 2007. Autoantigen-B cell antigen receptor interactions that regulate expression of B cell antigen receptor Loci. J Immunol 178(8):5035-47. [PubMed: 17404286]  [MGI Ref ID J:145282]

Lo CG; Xu Y; Proia RL; Cyster JG. 2005. Cyclical modulation of sphingosine-1-phosphate receptor 1 surface expression during lymphocyte recirculation and relationship to lymphoid organ transit. J Exp Med 201(2):291-301. [PubMed: 15657295]  [MGI Ref ID J:95700]

Ludwig-Portugall I; Hamilton-Williams EE; Gottschalk C; Kurts C. 2008. Cutting Edge: CD25+ regulatory T cells prevent expansion and induce apoptosis of B cells specific for tissue autoantigens. J Immunol 181(7):4447-51. [PubMed: 18802046]  [MGI Ref ID J:141302]

Macaulay AE; DeKruyff RH; Umetsu DT. 1998. Antigen-primed T cells from B cell-deficient JHD mice fail to provide B cell help. J Immunol 160(4):1694-700. [PubMed: 9469426]  [MGI Ref ID J:111514]

Macauley MS; Pfrengle F; Rademacher C; Nycholat CM; Gale AJ; von Drygalski A; Paulson JC. 2013. Antigenic liposomes displaying CD22 ligands induce antigen-specific B cell apoptosis. J Clin Invest 123(7):3074-83. [PubMed: 23722906]  [MGI Ref ID J:201430]

Madureira P; Baptista M; Vieira M; Magalhaes V; Camelo A; Oliveira L; Ribeiro A; Tavares D; Trieu-Cuot P; Vilanova M; Ferreira P. 2007. Streptococcus agalactiae GAPDH is a virulence-associated immunomodulatory protein. J Immunol 178(3):1379-87. [PubMed: 17237385]  [MGI Ref ID J:143659]

Manderson AP; Quah B; Botto M; Goodnow CC; Walport MJ; Parish CR. 2006. A novel mechanism for complement activation at the surface of B cells following antigen binding. J Immunol 177(8):5155-62. [PubMed: 17015700]  [MGI Ref ID J:139451]

Mandik-Nayak L; Racz J; Sleckman BP; Allen PM. 2006. Autoreactive marginal zone B cells are spontaneously activated but lymph node B cells require T cell help. J Exp Med 203(8):1985-98. [PubMed: 16880262]  [MGI Ref ID J:124390]

Marino E; Tan B; Binge L; Mackay CR; Grey ST. 2012. B-cell cross-presentation of autologous antigen precipitates diabetes. Diabetes 61(11):2893-905. [PubMed: 22829452]  [MGI Ref ID J:208509]

Marques R; Antunes I; Eksmond U; Stoye J; Hasenkrug K; Kassiotis G. 2008. B lymphocyte activation by coinfection prevents immune control of friend virus infection. J Immunol 181(5):3432-40. [PubMed: 18714015]  [MGI Ref ID J:142292]

McClellan KB; Gangappa S; Speck SH; Virgin HW 4th. 2006. Antibody-independent control of gamma-herpesvirus latency via B cell induction of anti-viral T cell responses. PLoS Pathog 2(6):e58. [PubMed: 16789842]  [MGI Ref ID J:162218]

McDonald KG; McDonough JS; Newberry RD. 2005. Adaptive immune responses are dispensable for isolated lymphoid follicle formation: antigen-naive, lymphotoxin-sufficient B lymphocytes drive the formation of mature isolated lymphoid follicles. J Immunol 174(9):5720-8. [PubMed: 15843574]  [MGI Ref ID J:98463]

McGehee AM; Dougan SK; Klemm EJ; Shui G; Park B; Kim YM; Watson N; Wenk MR; Ploegh HL; Hu CC. 2009. XBP-1-deficient plasmablasts show normal protein folding but altered glycosylation and lipid synthesis. J Immunol 183(6):3690-9. [PubMed: 19710472]  [MGI Ref ID J:152296]

McPherson M; Wei B; Turovskaya O; Fujiwara D; Brewer S; Braun J. 2008. Colitis immunoregulation by CD8+ T cell requires T cell cytotoxicity and B cell peptide antigen presentation. Am J Physiol Gastrointest Liver Physiol 295(3):G485-92. [PubMed: 18617557]  [MGI Ref ID J:141865]

Mecklenbrauker I; Saijo K; Zheng NY; Leitges M; Tarakhovsky A. 2002. Protein kinase Cdelta controls self-antigen-induced B-cell tolerance. Nature 416(6883):860-5. [PubMed: 11976686]  [MGI Ref ID J:76134]

Meffre E; Nussenzweig MC. 2002. Deletion of immunoglobulin beta in developing B cells leads to cell death. Proc Natl Acad Sci U S A 99(17):11334-9. [PubMed: 12165571]  [MGI Ref ID J:78601]

Merrell KT; Benschop RJ; Gauld SB; Aviszus K; Decote-Ricardo D; Wysocki LJ; Cambier JC. 2006. Identification of anergic B cells within a wild-type repertoire. Immunity 25(6):953-62. [PubMed: 17174121]  [MGI Ref ID J:116779]

Miles K; Heaney J; Sibinska Z; Salter D; Savill J; Gray D; Gray M. 2011. A tolerogenic role for Toll-like receptor 9 is revealed by B-cell interaction with DNA complexes expressed on apoptotic cells. Proc Natl Acad Sci U S A :. [PubMed: 22207622]  [MGI Ref ID J:179971]

Miller AT; Beisner DR; Liu D; Cooke MP. 2009. Inositol 1,4,5-trisphosphate 3-kinase B is a negative regulator of BCR signaling that controls B cell selection and tolerance induction. J Immunol 182(8):4696-704. [PubMed: 19342645]  [MGI Ref ID J:147739]

Nijnik A; Dawson S; Crockford TL; Woodbine L; Visetnoi S; Bennett S; Jones M; Turner GD; Jeggo PA; Goodnow CC; Cornall RJ. 2009. Impaired lymphocyte development and antibody class switching and increased malignancy in a murine model of DNA ligase IV syndrome. J Clin Invest 119(6):1696-705. [PubMed: 19451691]  [MGI Ref ID J:150573]

O'Neill SK; Getahun A; Gauld SB; Merrell KT; Tamir I; Smith MJ; Dal Porto JM; Li QZ; Cambier JC. 2011. Monophosphorylation of CD79a and CD79b ITAM Motifs Initiates a SHIP-1 Phosphatase-Mediated Inhibitory Signaling Cascade Required for B Cell Anergy. Immunity 35(5):746-56. [PubMed: 22078222]  [MGI Ref ID J:178833]

Okada T; Miller MJ; Parker I; Krummel MF; Neighbors M; Hartley SB; O'Garra A; Cahalan MD; Cyster JG. 2005. Antigen-engaged B cells undergo chemotaxis toward the T zone and form motile conjugates with helper T cells. PLoS Biol 3(6):e150. [PubMed: 15857154]  [MGI Ref ID J:99611]

Okuyama K; Murata A; Sudo T; Yoshino M; Hayashi S. 2012. A checkpoint in B-lymphopoiesis related to Notch resistance. Biochem Biophys Res Commun 417(1):141-6. [PubMed: 22138652]  [MGI Ref ID J:180314]

Patakas A; Benson RA; Withers DR; Conigliaro P; McInnes IB; Brewer JM; Garside P. 2012. Th17 effector cells support B cell responses outside of germinal centres. PLoS One 7(11):e49715. [PubMed: 23166752]  [MGI Ref ID J:195462]

Pathak S; Ma S; Shukla V; Lu R. 2013. A role for IRF8 in B cell anergy. J Immunol 191(12):6222-30. [PubMed: 24218455]  [MGI Ref ID J:207133]

Pathak S; Ma S; Trinh L; Lu R. 2008. A role for interferon regulatory factor 4 in receptor editing. Mol Cell Biol 28(8):2815-24. [PubMed: 18285461]  [MGI Ref ID J:133924]

Patterson HC; Kraus M; Kim YM; Ploegh H; Rajewsky K. 2006. The B cell receptor promotes B cell activation and proliferation through a non-ITAM tyrosine in the Igalpha cytoplasmic domain. Immunity 25(1):55-65. [PubMed: 16860757]  [MGI Ref ID J:113407]

Pereira JP; Kelly LM; Xu Y; Cyster JG. 2009. EBI2 mediates B cell segregation between the outer and centre follicle. Nature :. [PubMed: 19597478]  [MGI Ref ID J:151077]

Pfrengle F; Macauley MS; Kawasaki N; Paulson JC. 2013. Copresentation of antigen and ligands of Siglec-G induces B cell tolerance independent of CD22. J Immunol 191(4):1724-31. [PubMed: 23836061]  [MGI Ref ID J:205703]

Phan TG; Amesbury M; Gardam S; Crosbie J; Hasbold J; Hodgkin PD; Basten A; Brink R. 2003. B cell receptor-independent stimuli trigger immunoglobulin (Ig) class switch recombination and production of IgG autoantibodies by anergic self-reactive B cells. J Exp Med 197(7):845-60. [PubMed: 12668643]  [MGI Ref ID J:132538]

Ploquin MJ; Eksmond U; Kassiotis G. 2011. B cells and TCR avidity determine distinct functions of CD4+ T cells in retroviral infection. J Immunol 187(6):3321-30. [PubMed: 21841129]  [MGI Ref ID J:179252]

Pluger EB; Boes M; Alfonso C; Schroter CJ; Kalbacher H; Ploegh HL; Driessen C. 2002. Specific role for cathepsin S in the generation of antigenic peptides in vivo. Eur J Immunol 32(2):467-76. [PubMed: 11813165]  [MGI Ref ID J:107213]

Poe JC; Kountikov EI; Lykken JM; Natarajan A; Marchuk DA; Tedder TF. 2014. EndoU is a novel regulator of AICD during peripheral B cell selection. J Exp Med 211(1):57-69. [PubMed: 24344237]  [MGI Ref ID J:208364]

Poholek AC; Hansen K; Hernandez SG; Eto D; Chandele A; Weinstein JS; Dong X; Odegard JM; Kaech SM; Dent AL; Crotty S; Craft J. 2010. In vivo regulation of Bcl6 and T follicular helper cell development. J Immunol 185(1):313-26. [PubMed: 20519643]  [MGI Ref ID J:161433]

Prazma CM; Yazawa N; Fujimoto Y; Fujimoto M; Tedder TF. 2007. CD83 expression is a sensitive marker of activation required for B cell and CD4+ T cell longevity in vivo. J Immunol 179(7):4550-62. [PubMed: 17878352]  [MGI Ref ID J:152350]

Qi H; Cannons JL; Klauschen F; Schwartzberg PL; Germain RN. 2008. SAP-controlled T-B cell interactions underlie germinal centre formation. Nature 455(7214):764-9. [PubMed: 18843362]  [MGI Ref ID J:140086]

Quah BJ; Barlow VP; McPhun V; Matthaei KI; Hulett MD; Parish CR. 2008. Bystander B cells rapidly acquire antigen receptors from activated B cells by membrane transfer. Proc Natl Acad Sci U S A 105(11):4259-64. [PubMed: 18337504]  [MGI Ref ID J:173514]

Quong MW; Harris DP; Swain SL; Murre C. 1999. E2A activity is induced during B-cell activation to promote immunoglobulin class switch recombination. EMBO J 18(22):6307-18. [PubMed: 10562543]  [MGI Ref ID J:118965]

Refaeli Y; Young RM; Turner BC; Duda J; Field KA; Bishop JM. 2008. The B cell antigen receptor and overexpression of MYC can cooperate in the genesis of B cell lymphomas. PLoS Biol 6(6):e152. [PubMed: 18578569]  [MGI Ref ID J:139351]

Reif K; Okkenhaug K; Sasaki T; Penninger JM; Vanhaesebroeck B; Cyster JG. 2004. Cutting edge: differential roles for phosphoinositide 3-kinases, p110gamma and p110delta, in lymphocyte chemotaxis and homing. J Immunol 173(4):2236-40. [PubMed: 15294934]  [MGI Ref ID J:92733]

Rivera A; Chen CC; Ron N; Dougherty JP; Ron Y. 2001. Role of B cells as antigen-presenting cells in vivo revisited: antigen-specific B cells are essential for T cell expansion in lymph nodes and for systemic T cell responses to low antigen concentrations. Int Immunol 13(12):1583-93. [PubMed: 11717199]  [MGI Ref ID J:107395]

Roldan E; Fuxa M; Chong W; Martinez D; Novatchkova M; Busslinger M; Skok JA. 2005. Locus 'decontraction' and centromeric recruitment contribute to allelic exclusion of the immunoglobulin heavy-chain gene. Nat Immunol 6(1):31-41. [PubMed: 15580273]  [MGI Ref ID J:134531]

Roy V; Bonventi G; Cai Y; Macleod R; Wither JE. 2007. Immune mechanisms leading to abnormal B cell selection and activation in New Zealand Black mice. Eur J Immunol 37(9):2645-56. [PubMed: 17668901]  [MGI Ref ID J:124351]

Roy V; Chang NH; Cai Y; Bonventi G; Wither J. 2005. Aberrant IgM signaling promotes survival of transitional T1 B cells and prevents tolerance induction in lupus-prone New Zealand black mice. J Immunol 175(11):7363-71. [PubMed: 16301643]  [MGI Ref ID J:122135]

Rubtsova K; Rubtsov AV; van Dyk LF; Kappler JW; Marrack P. 2013. T-box transcription factor T-bet, a key player in a unique type of B-cell activation essential for effective viral clearance. Proc Natl Acad Sci U S A 110(34):E3216-24. [PubMed: 23922396]  [MGI Ref ID J:200754]

Saez de Guinoa J; Barrio L; Mellado M; Carrasco YR. 2011. CXCL13/CXCR5 signaling enhances BCR-triggered B-cell activation by shaping cell dynamics. Blood 118(6):1560-9. [PubMed: 21659539]  [MGI Ref ID J:176933]

Schram BR; Tze LE; Ramsey LB; Liu J; Najera L; Vegoe AL; Hardy RR; Hippen KL; Farrar MA; Behrens TW. 2008. B cell receptor Basal signaling regulates antigen-induced Ig light chain rearrangements. J Immunol 180(7):4728-41. [PubMed: 18354197]  [MGI Ref ID J:133383]

Sharbeen G; Yee CW; Smith AL; Jolly CJ. 2012. Ectopic restriction of DNA repair reveals that UNG2 excises AID-induced uracils predominantly or exclusively during G1 phase. J Exp Med 209(5):965-74. [PubMed: 22529268]  [MGI Ref ID J:185140]

Shimomura Y; Ogawa A; Kawada M; Sugimoto K; Mizoguchi E; Shi HN; Pillai S; Bhan AK; Mizoguchi A. 2008. A unique B2 B cell subset in the intestine. J Exp Med 205(6):1343-55. [PubMed: 18519649]  [MGI Ref ID J:137039]

Silva DG; Daley SR; Hogan J; Lee SK; Teh CE; Hu DY; Lam KP; Goodnow CC; Vinuesa CG. 2011. Anti-islet autoantibodies trigger autoimmune diabetes in the presence of an increased frequency of islet-reactive CD4 T cells. Diabetes 60(8):2102-11. [PubMed: 21788582]  [MGI Ref ID J:186792]

Silveira PA; Chapman HD; Stolp J; Johnson E; Cox SL; Hunter K; Wicker LS; Serreze DV. 2006. Genes within the Idd5 and Idd9/11 Diabetes Susceptibility Loci Affect the Pathogenic Activity of B Cells in Nonobese Diabetic Mice. J Immunol 177(10):7033-41. [PubMed: 17082619]  [MGI Ref ID J:114754]

Silveira PA; Dombrowsky J; Johnson E; Chapman HD; Nemazee D; Serreze DV. 2004. B cell selection defects underlie the development of diabetogenic APCs in nonobese diabetic mice. J Immunol 172(8):5086-94. [PubMed: 15067092]  [MGI Ref ID J:89156]

Silveira PA; Johnson E; Chapman HD; Bui T; Tisch RM; Serreze DV. 2002. The preferential ability of B lymphocytes to act as diabetogenic APC in NOD mice depends on expression of self-antigen-specific immunoglobulin receptors. Eur J Immunol 32(12):3657-66. [PubMed: 12516557]  [MGI Ref ID J:80859]

Silver K; Ferry H; Crockford T; Cornall RJ. 2006. TLR4, TLR9 and MyD88 are not required for the positive selection of autoreactive B cells into the primary repertoire. Eur J Immunol 36(6):1404-12. [PubMed: 16703567]  [MGI Ref ID J:115059]

Skelsey ME; Mayhew E; Niederkorn JY. 2003. Splenic B cells act as antigen presenting cells for the induction of anterior chamber-associated immune deviation. Invest Ophthalmol Vis Sci 44(12):5242-51. [PubMed: 14638723]  [MGI Ref ID J:107362]

Stolp J; Marino E; Batten M; Sierro F; Cox SL; Grey ST; Silveira PA. 2013. Intrinsic molecular factors cause aberrant expansion of the splenic marginal zone B cell population in nonobese diabetic mice. J Immunol 191(1):97-109. [PubMed: 23740954]  [MGI Ref ID J:205458]

Straub T; Schweier O; Bruns M; Nimmerjahn F; Waisman A; Pircher H. 2013. Nucleoprotein-specific nonneutralizing antibodies speed up LCMV elimination independently of complement and FcgammaR. Eur J Immunol 43(9):2338-48. [PubMed: 23749409]  [MGI Ref ID J:201330]

Su YW; Chen YP; Chen MY; Reth M; Tan TH. 2013. The serine/threonine phosphatase PP4 is required for pro-B cell development through its promotion of immunoglobulin VDJ recombination. PLoS One 8(7):e68804. [PubMed: 23874770]  [MGI Ref ID J:204399]

Swanson-Mungerson M; Bultema R; Longnecker R. 2006. Epstein-Barr virus LMP2A enhances B-cell responses in vivo and in vitro. J Virol 80(14):6764-70. [PubMed: 16809282]  [MGI Ref ID J:147405]

Taylor JJ; Martinez RJ; Titcombe PJ; Barsness LO; Thomas SR; Zhang N; Katzman SD; Jenkins MK; Mueller DL. 2012. Deletion and anergy of polyclonal B cells specific for ubiquitous membrane-bound self-antigen. J Exp Med 209(11):2065-77. [PubMed: 23071255]  [MGI Ref ID J:190897]

Tsitoura DC; Yeung VP; DeKruyff RH; Umetsu DT. 2002. Critical role of B cells in the development of T cell tolerance to aeroallergens. Int Immunol 14(6):659-67. [PubMed: 12039917]  [MGI Ref ID J:133557]

Tze LE; Schram BR; Lam KP; Hogquist KA; Hippen KL; Liu J; Shinton SA; Otipoby KL; Rodine PR; Vegoe AL; Kraus M; Hardy RR; Schlissel MS; Rajewsky K; Behrens TW. 2005. Basal immunoglobulin signaling actively maintains developmental stage in immature B cells. PLoS Biol 3(3):e82. [PubMed: 15752064]  [MGI Ref ID J:133822]

Velazquez P; Wei B; McPherson M; Mendoza LM; Nguyen SL; Turovskaya O; Kronenberg M; Huang TT; Schrage M; Lobato LN; Fujiwara D; Brewer S; Arditi M; Cheng G; Sartor RB; Newberry RD; Braun J. 2008. Villous B cells of the small intestine are specialized for invariant NK T cell dependence. J Immunol 180(7):4629-38. [PubMed: 18354186]  [MGI Ref ID J:133099]

Wang JQ; Jeelall YS; Beutler B; Horikawa K; Goodnow CC. 2014. Consequences of the recurrent MYD88(L265P) somatic mutation for B cell tolerance. J Exp Med 211(3):413-26. [PubMed: 24534189]  [MGI Ref ID J:210476]

Weber M; Treanor B; Depoil D; Shinohara H; Harwood NE; Hikida M; Kurosaki T; Batista FD. 2008. Phospholipase C-gamma2 and Vav cooperate within signaling microclusters to propagate B cell spreading in response to membrane-bound antigen. J Exp Med 205(4):853-68. [PubMed: 18362175]  [MGI Ref ID J:133999]

Wen R; Chen Y; Schuman J; Fu G; Yang S; Zhang W; Newman DK; Wang D. 2004. An important role of phospholipase Cgamma1 in pre-B-cell development and allelic exclusion. EMBO J 23(20):4007-17. [PubMed: 15372077]  [MGI Ref ID J:93280]

Wheeler ML; Defranco AL. 2012. Prolonged production of reactive oxygen species in response to B cell receptor stimulation promotes B cell activation and proliferation. J Immunol 189(9):4405-16. [PubMed: 23024271]  [MGI Ref ID J:190609]

Winslow MM; Gallo EM; Neilson JR; Crabtree GR. 2006. The calcineurin phosphatase complex modulates immunogenic B cell responses. Immunity 24(2):141-52. [PubMed: 16473827]  [MGI Ref ID J:113318]

Wojciechowski W; Harris DP; Sprague F; Mousseau B; Makris M; Kusser K; Honjo T; Mohrs K; Mohrs M; Randall T; Lund FE. 2009. Cytokine-producing effector B cells regulate type 2 immunity to H. polygyrus. Immunity 30(3):421-33. [PubMed: 19249230]  [MGI Ref ID J:146768]

Wong MX; Hayball JD; Jackson DE. 2008. PECAM-1-regulated signalling thresholds control tolerance in anergic transgenic B-cells. Mol Immunol 45(6):1767-81. [PubMed: 17977600]  [MGI Ref ID J:131644]

Yabas M; Teh CE; Frankenreiter S; Lal D; Roots CM; Whittle B; Andrews DT; Zhang Y; Teoh NC; Sprent J; Tze LE; Kucharska EM; Kofler J; Farell GC; Broer S; Goodnow CC; Enders A. 2011. ATP11C is critical for the internalization of phosphatidylserine and differentiation of B lymphocytes. Nat Immunol 12(5):441-9. [PubMed: 21423173]  [MGI Ref ID J:171924]

Yanaba K; Bouaziz JD; Matsushita T; Tsubata T; Tedder TF. 2009. The development and function of regulatory B cells expressing IL-10 (B10 cells) requires antigen receptor diversity and TLR signals. J Immunol 182(12):7459-72. [PubMed: 19494269]  [MGI Ref ID J:149301]

Yau IW; Cato MH; Jellusova J; Hurtado de Mendoza T; Brink R; Rickert RC. 2013. Censoring of self-reactive B cells by follicular dendritic cell-displayed self-antigen. J Immunol 191(3):1082-90. [PubMed: 23817432]  [MGI Ref ID J:205710]

Yi T; Wang X; Kelly LM; An J; Xu Y; Sailer AW; Gustafsson JA; Russell DW; Cyster JG. 2012. Oxysterol Gradient Generation by Lymphoid Stromal Cells Guides Activated B Cell Movement during Humoral Responses. Immunity 37(3):535-48. [PubMed: 22999953]  [MGI Ref ID J:188276]

Yoshizaki A; Miyagaki T; DiLillo DJ; Matsushita T; Horikawa M; Kountikov EI; Spolski R; Poe JC; Leonard WJ; Tedder TF. 2012. Regulatory B cells control T-cell autoimmunity through IL-21-dependent cognate interactions. Nature 491(7423):264-8. [PubMed: 23064231]  [MGI Ref ID J:189218]

Young RM; Hardy IR; Clarke RL; Lundy N; Pine P; Turner BC; Potter TA; Refaeli Y. 2009. Mouse models of non-Hodgkin lymphoma reveal Syk as an important therapeutic target. Blood 113(11):2508-16. [PubMed: 18981293]  [MGI Ref ID J:146340]

Young RM; Polsky A; Refaeli Y. 2009. TC-PTP is required for the maintenance of MYC-driven B-cell lymphomas. Blood 114(24):5016-23. [PubMed: 19755676]  [MGI Ref ID J:155497]

Yu M; Chen Y; He Y; Podd A; Fu G; Wright JA; Kleiman E; Khan WN; Wen R; Wang D. 2012. Critical role of B cell lymphoma 10 in BAFF-regulated NF-kappaB activation and survival of anergic B cells. J Immunol 189(11):5185-93. [PubMed: 23087406]  [MGI Ref ID J:190683]

Yuseff MI; Reversat A; Lankar D; Diaz J; Fanget I; Pierobon P; Randrian V; Larochette N; Vascotto F; Desdouets C; Jauffred B; Bellaiche Y; Gasman S; Darchen F; Desnos C; Lennon-Dumenil AM. 2011. Polarized secretion of lysosomes at the B cell synapse couples antigen extraction to processing and presentation. Immunity 35(3):361-74. [PubMed: 21820334]  [MGI Ref ID J:176430]

Ziegler AI; Le Page MA; Maxwell MJ; Stolp J; Guo H; Jayasimhan A; Hibbs ML; Santamaria P; Miller JF; Plebanski M; Silveira PA; Slattery RM. 2013. The CD19 signalling molecule is elevated in NOD mice and controls type 1 diabetes development. Diabetologia 56(12):2659-68. [PubMed: 24013782]  [MGI Ref ID J:203810]

Zikherman J; Doan K; Parameswaran R; Raschke W; Weiss A. 2012. Quantitative differences in CD45 expression unmask functions for CD45 in B-cell development, tolerance, and survival. Proc Natl Acad Sci U S A 109(1):E3-12. [PubMed: 22135465]  [MGI Ref ID J:180527]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Breeding & HusbandryThe transgene is derived from BALB/c mice and has an IgHa allotype, while the recipient C57BL/6 mice have an IgHb allotype. Both light and heavy chain genes specific for hen egg lysozyme were injected into the B6 eggs. The transgene consists of Igkc (kappa chain constant region) and rearranged Ighm (IgM) and Ighd (IgD) heavy chains specific for hen egg lysozyme (HEL). The donating investigator reports that the transgene integrated on Chromosome 17.
Mating SystemInbred x Hemizygote         (Female x Male)   01-MAR-06
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHemizygous for Tg(IghelMD4)4Ccg  
Price per Pair (US dollars $)Pair Genotype
$254.55C57BL/6J (000664) x Hemizygous for Tg(IghelMD4)4Ccg  
$253.05Hemizygous for Tg(IghelMD4)4Ccg x C57BL/6J (000664)  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHemizygous for Tg(IghelMD4)4Ccg  
Price per Pair (US dollars $)Pair Genotype
$331.00C57BL/6J (000664) x Hemizygous for Tg(IghelMD4)4Ccg  
$329.00Hemizygous for Tg(IghelMD4)4Ccg x C57BL/6J (000664)  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.6)