Strain Name:

STOCK Tg(TcrHEL3A9)1Mmd/J

Stock Number:

002597

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names STOCK Tg(TcrHEL3A9)Mmd/J    (Changed: 16-MAY-08 )
Type Mutant Stock; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Mating System+/+ sibling x Hemizygote         (Female x Male)   17-MAY-08
Specieslaboratory mouse
 
Donating InvestigatorDr. Mark Davis,   Stanford University School of Medicine

Appearance
black
Related Genotype: a/a

Description
Mice carrying the (TcrHEL3A9) transgene express the alpha and beta chains of a T-cell receptor specific for hen egg lysozyme. Transgenics are used to study maintenance and breakdown of immunological tolerance, overcoming of the anergic state, and issues of costimuli.

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Tcra
005308   B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005895   B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J
002761   B10.Cg-Tg(TcrAND)53Hed/J
003147   B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Dlo/J
003199   B10.PL-H2u H2-T18a/(73NS)Sn-Tg(TCRA)B1Jg/J
002116   B6.129S2-Tcratm1Mom/J
022073   B6.Cg-Rag1tm1Mom Thy1a Tg(Tcra2C,Tcrb2C)1Dlo/J
008684   B6.Cg-Rag1tm1Mom Tyrp1B-w Tg(Tcra,Tcrb)9Rest/J
014550   B6.Cg-Thy1a Tg(TcraCWM5,TcrbCWM5)1807Wuth/J
005023   B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J
005655   B6.Cg-Tg(Tcra,Tcrb)3Ayr/J
008428   B6.Cg-Tg(Tcra,Tcrb)HRCAll/J
008429   B6.Cg-Tg(Tcra,Tcrb)HRVAll/J
008006   B6.Cg-Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr/J
004194   B6.Cg-Tg(TcraTcrb)425Cbn/J
005236   B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J
004554   B6.NOD-(D17Mit21-D17Mit10) Tg(TCRaAI4)1Dvs/DvsJ
002115   B6;129S2-Tcratm1Mom/J
004694   B6;D2-Tg(TcrLCMV)327Sdz/JDvsJ
002408   B6;SJL-Tg(TcrAND)53Hed/J
007848   BXSB.129P2(Cg)-Tcratm1Mjo/TheoJ
021880   BXSB.B6-Tg(TcraTcrb)1100Mjb/DcrJ
004364   C.Cg-Tcratm1Mom Tcrbtm1Mom/J
003303   C.Cg-Tg(DO11.10)10Dlo/J
002045   C.SJL-Tcrac/SlkJ
002047   C.SJL-Tcrba Tcrac/SlkJ
014639   C57BL/6-Tg(Cd4-TcraDN32D3)1Aben/J
011005   C57BL/6-Tg(H2-Kb-Tcra,-Tcrb)P25Ktk/J
006912   C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J
003831   C57BL/6-Tg(TcraTcrb)1100Mjb/J
005307   CBy.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005922   CBy.Cg-Thy1a Tg(TcraCl1,TcrbCl1)1Shrm/J
005694   D1Lac.Cg-Tg(Tcra,Tcrb)24Efro/J
017314   NOD-Tg(TcraTcrb)2H6Lwn/J
004444   NOD.129P2(C)-Tcratm1Mjo/DoiJ
006436   NOD.Cg-(Gpi1-D7Mit346)C57BL/6J Tg(TcraAI4)1Dvs/DvsJ
004257   NOD.Cg-Prkdcscid Tg(TcrLCMV)327Sdz/DvsJ
004347   NOD.Cg-Rag1tm1Mom Tg(TcraAI4)1Dvs/DvsJ
009377   NOD.Cg-Rag1tm1Mom Tg(TcraBDC12-4.1)10Jos Tg(TcrbBDC12-4.1)82Gse/J
024476   NOD.Cg-Stat4tm1Gru Thy1a Ifngr1tm1Agt Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/LmbrJ
005686   NOD.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
004696   NOD.Cg-Tg(TcrLCMV)327Sdz/DvsJ
004460   NOD.Cg-Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/DoiJ
010526   NOD.Cg-Tg(TcraTcrbNY4.1)1Pesa/DvsJ
005868   NOD.Cg-Tg(TcraTcrbNY8.3)1Pesa/DvsJ
006303   NOD.FVB-Tg(TcraBDC12-4.1)10Jos/GseJ
004334   NOD/ShiLt-Tg(TcraAI4)1Dvs
018030   SJL.Cg-Tg(TcraTcrbVP2)1Bkim/J
View Strains carrying other alleles of Tcra     (48 strains)

Strains carrying other alleles of Tcrb
005308   B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005895   B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J
002761   B10.Cg-Tg(TcrAND)53Hed/J
003147   B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Dlo/J
003200   B10.PL-H2u H2-T18a/(73NS)Sn-Tg(TCRB)C14Jg/J
002122   B6.129P2-Tcrbtm1Mom Tcrdtm1Mom/J
002118   B6.129P2-Tcrbtm1Mom/J
022073   B6.Cg-Rag1tm1Mom Thy1a Tg(Tcra2C,Tcrb2C)1Dlo/J
008684   B6.Cg-Rag1tm1Mom Tyrp1B-w Tg(Tcra,Tcrb)9Rest/J
014550   B6.Cg-Thy1a Tg(TcraCWM5,TcrbCWM5)1807Wuth/J
005023   B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J
005655   B6.Cg-Tg(Tcra,Tcrb)3Ayr/J
008428   B6.Cg-Tg(Tcra,Tcrb)HRCAll/J
008429   B6.Cg-Tg(Tcra,Tcrb)HRVAll/J
008006   B6.Cg-Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr/J
004194   B6.Cg-Tg(TcraTcrb)425Cbn/J
005236   B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J
008430   B6.Cg-Tg(Tcrb)HRBAll/J
004555   B6.NOD-(D17Mit21-D17Mit10) Tg(TCRbAI4)1Dvs/DvsJ
002121   B6;129P-Tcrbtm1Mom Tcrdtm1Mom/J
002117   B6;129P2-Tcrbtm1Mom/J
004694   B6;D2-Tg(TcrLCMV)327Sdz/JDvsJ
002408   B6;SJL-Tg(TcrAND)53Hed/J
021880   BXSB.B6-Tg(TcraTcrb)1100Mjb/DcrJ
004364   C.Cg-Tcratm1Mom Tcrbtm1Mom/J
003303   C.Cg-Tg(DO11.10)10Dlo/J
002047   C.SJL-Tcrba Tcrac/SlkJ
002046   C.SJL-Tcrba/SlkJ
011005   C57BL/6-Tg(H2-Kb-Tcra,-Tcrb)P25Ktk/J
006912   C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J
003831   C57BL/6-Tg(TcraTcrb)1100Mjb/J
003540   C57L/J-Tg(Tcrb)93Vbo/J
005307   CBy.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005922   CBy.Cg-Thy1a Tg(TcraCl1,TcrbCl1)1Shrm/J
007081   CByJ.129P2(B6)-Tcrbtm1Mom/J
005694   D1Lac.Cg-Tg(Tcra,Tcrb)24Efro/J
017314   NOD-Tg(TcraTcrb)2H6Lwn/J
023082   NOD.129P2(Cg)-Tcrbtm1Mom/MnkaJ
006437   NOD.Cg-(Gpi1-D7Mit346)C57BL/6J Tg(TcrbAI4)1Dvs/DvsJ
004257   NOD.Cg-Prkdcscid Tg(TcrLCMV)327Sdz/DvsJ
009377   NOD.Cg-Rag1tm1Mom Tg(TcraBDC12-4.1)10Jos Tg(TcrbBDC12-4.1)82Gse/J
024476   NOD.Cg-Stat4tm1Gru Thy1a Ifngr1tm1Agt Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/LmbrJ
005686   NOD.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
004696   NOD.Cg-Tg(TcrLCMV)327Sdz/DvsJ
004460   NOD.Cg-Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/DoiJ
010526   NOD.Cg-Tg(TcraTcrbNY4.1)1Pesa/DvsJ
005868   NOD.Cg-Tg(TcraTcrbNY8.3)1Pesa/DvsJ
006304   NOD.FVB-Tg(TcrbBDC12-4.1)82Gse/GseJ
004335   NOD/ShiLt-Tg(TcrbAI4)1Dvs
018030   SJL.Cg-Tg(TcraTcrbVP2)1Bkim/J
View Strains carrying other alleles of Tcrb     (50 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Tg(TcrHEL3A9)1Mmd/0

        involves: C57BL/6
  • immune system phenotype
  • abnormal CD4-positive, alpha beta T cell morphology
    • vast majority of CD4 T cells express a T cell receptor that is specific for hen egg lysozyme (HEL) antigen   (MGI Ref ID J:73608)
  • abnormal T cell activation
    • CD4 T cells become activated when cultured with HEL protein and B cells from Tg(IghelMD4)4Ccg transgenic mice   (MGI Ref ID J:73608)
    • 12 hours of culture leads to upregulation of CD69 on the cell surface   (MGI Ref ID J:73608)
    • CD4 T cells enlarge into blasts and express high levels of CD44 after 48 hours in culture   (MGI Ref ID J:73608)
    • supernatant from these cultures supports a IL-2/IL-4 dependent cell line   (MGI Ref ID J:73608)
    • the use of HEL peptide leads to similar but somewhat reduced levels of activation   (MGI Ref ID J:73608)
    • increased T cell proliferation   (MGI Ref ID J:73608)
  • abnormal interleukin secretion
    • CD4 T cells become activated and secrete cytokines when cultured with HEL protein and B cells from Tg(IghelMD4)4Ccg transgenic mice   (MGI Ref ID J:73608)
    • supernatant from these cultures supports a IL-2/IL-4 dependent cell line   (MGI Ref ID J:73608)
  • hematopoietic system phenotype
  • abnormal CD4-positive, alpha beta T cell morphology
    • vast majority of CD4 T cells express a T cell receptor that is specific for hen egg lysozyme (HEL) antigen   (MGI Ref ID J:73608)
  • abnormal T cell activation
    • CD4 T cells become activated when cultured with HEL protein and B cells from Tg(IghelMD4)4Ccg transgenic mice   (MGI Ref ID J:73608)
    • 12 hours of culture leads to upregulation of CD69 on the cell surface   (MGI Ref ID J:73608)
    • CD4 T cells enlarge into blasts and express high levels of CD44 after 48 hours in culture   (MGI Ref ID J:73608)
    • supernatant from these cultures supports a IL-2/IL-4 dependent cell line   (MGI Ref ID J:73608)
    • the use of HEL peptide leads to similar but somewhat reduced levels of activation   (MGI Ref ID J:73608)
    • increased T cell proliferation   (MGI Ref ID J:73608)

Tg(TcrHEL3A9)1Mmd/0

        involves: C57BL/6 * C57BL/10 * C57BR/cd
  • immune system phenotype
  • abnormal CD4-positive, alpha beta T cell morphology
    • 15% of total lymphocytes found in lymph nodes are CD4 T cells bearing the transgenic TCR   (MGI Ref ID J:78309)
    • CD4 T cells that express the transgenic TCR-beta chain also express low or high amounts of the TCR-alpha chain   (MGI Ref ID J:78309)
    • the CD4 T cells expressing the low amounts of transgenic TCR-alpha may also express endogenous TCR-alpha chains   (MGI Ref ID J:78309)
    • decreased CD4-positive, alpha beta T cell number
      • CD 4 T cell numbers in the lymph nodes are reduced by about a third   (MGI Ref ID J:78309)
  • abnormal T cell activation
    • in vitro TCR stimulation leads to reduced expression of CD69 compared to T cells from mice transgenic for just the TCR   (MGI Ref ID J:78309)
    • abnormal T cell proliferation
      • CD4 T cells expressing the transgenic TCR proliferate in response to hen egg lysozyme   (MGI Ref ID J:78309)
      • proliferation occurs whether CD4 T cells are low or high expressers of the transgenic TCR-alpha chain   (MGI Ref ID J:78309)
  • abnormal double-positive T cell morphology
    • 55.9% of the double-positive thymocytes express the transgenic TCR   (MGI Ref ID J:78309)
  • abnormal thymus cell ratio
    • there is a skewing towards the CD-4 T cell subset in mice on a homozygote H2k/k background   (MGI Ref ID J:78309)
    • the ratio of T cell subsets in the thymus is skewed toward CD8 T cells on a heterozygote H2b/k background   (MGI Ref ID J:78309)
  • hematopoietic system phenotype
  • abnormal CD4-positive, alpha beta T cell morphology
    • 15% of total lymphocytes found in lymph nodes are CD4 T cells bearing the transgenic TCR   (MGI Ref ID J:78309)
    • CD4 T cells that express the transgenic TCR-beta chain also express low or high amounts of the TCR-alpha chain   (MGI Ref ID J:78309)
    • the CD4 T cells expressing the low amounts of transgenic TCR-alpha may also express endogenous TCR-alpha chains   (MGI Ref ID J:78309)
    • decreased CD4-positive, alpha beta T cell number
      • CD 4 T cell numbers in the lymph nodes are reduced by about a third   (MGI Ref ID J:78309)
  • abnormal T cell activation
    • in vitro TCR stimulation leads to reduced expression of CD69 compared to T cells from mice transgenic for just the TCR   (MGI Ref ID J:78309)
    • abnormal T cell proliferation
      • CD4 T cells expressing the transgenic TCR proliferate in response to hen egg lysozyme   (MGI Ref ID J:78309)
      • proliferation occurs whether CD4 T cells are low or high expressers of the transgenic TCR-alpha chain   (MGI Ref ID J:78309)
  • abnormal double-positive T cell morphology
    • 55.9% of the double-positive thymocytes express the transgenic TCR   (MGI Ref ID J:78309)
  • abnormal thymus cell ratio
    • there is a skewing towards the CD-4 T cell subset in mice on a homozygote H2k/k background   (MGI Ref ID J:78309)
    • the ratio of T cell subsets in the thymus is skewed toward CD8 T cells on a heterozygote H2b/k background   (MGI Ref ID J:78309)
  • endocrine/exocrine gland phenotype
  • abnormal thymus cell ratio
    • there is a skewing towards the CD-4 T cell subset in mice on a homozygote H2k/k background   (MGI Ref ID J:78309)
    • the ratio of T cell subsets in the thymus is skewed toward CD8 T cells on a heterozygote H2b/k background   (MGI Ref ID J:78309)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Rearranged Antigen-Specific T Cell Receptor Transgenes
      class II restricted

Research Tools
Immunology, Inflammation and Autoimmunity Research
      T Cell Receptor Transgenics

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(TcrHEL3A9)1Mmd
Allele Name transgene insertion 1, Mark M Davis
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) 3A9 TCR; 3A9 anti-HEL; Tg(TcrHEL3A9)Mmd;
Strain of OriginC57BL/6J
Expressed Gene Tcra, T cell receptor alpha chain, mouse, laboratory
Expressed Gene Tcrb, T cell receptor beta chain, mouse, laboratory
Promoter Tcra, T cell receptor alpha chain, mouse, laboratory
Promoter Tcrb, T cell receptor beta chain, mouse, laboratory
Molecular Note Transgenic mice produced from this construct carry transgenes encoding the T cell receptor alpha and beta (Tcra and Tcrb, respectively) chains from the hen egg lysozyme (HEL)-specific T cell hybridoma 3A9. Two vectors were constructed and subsequently co-injected into C57BL/6J eggs. The first vector contained the Tcra variable and joining regions (V and J, respectively) and a 3.5kb segment 3' to the Tcra constant region. The second vector contained the Tcrb variable, diversity, and joining regions (V, D, and J, respectively) and a 7.5kb segment 3' to the Tcrb constant region, which included the Tcrb enhancer region. Transgenic mice expressed the alpha and beta chains of a T cell receptor specific for hen egg lysozyme. Two female founders were generated. [MGI Ref ID J:73608]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(TcrHEL3A9)1Mmd, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Ho WY; Cooke MP; Goodnow CC; Davis MM. 1994. Resting and anergic B cells are defective in CD28-dependent costimulation of naive CD4+ T cells. J Exp Med 179(5):1539-49. [PubMed: 7909325]  [MGI Ref ID J:73608]

Additional References

Tg(TcrHEL3A9)1Mmd related

Akkaraju S; Ho WY; Leong D; Canaan K; Davis MM; Goodnow CC. 1997. A range of CD4 T cell tolerance: partial inactivation to organ-specific antigen allows nondestructive thyroiditis or insulitis. Immunity 7(2):255-71. [PubMed: 9285410]  [MGI Ref ID J:78309]

Altin JA; Daley SR; Howitt J; Rickards HJ; Batkin AK; Horikawa K; Prasad SJ; Nelms KA; Kumar S; Wu LC; Tan SS; Cook MC; Goodnow CC. 2014. Ndfip1 mediates peripheral tolerance to self and exogenous antigen by inducing cell cycle exit in responding CD4+ T cells. Proc Natl Acad Sci U S A 111(6):2067-74. [PubMed: 24520172]  [MGI Ref ID J:206813]

Atibalentja DF; Murphy KM; Unanue ER. 2011. Functional redundancy between thymic CD8alpha+ and Sirp alpha+ conventional dendritic cells in presentation of blood-derived lysozyme by MHC class II proteins. J Immunol 186(3):1421-31. [PubMed: 21178002]  [MGI Ref ID J:168924]

Calderon B; Carrero JA; Miller MJ; Unanue ER. 2011. Cellular and molecular events in the localization of diabetogenic T cells to islets of Langerhans. Proc Natl Acad Sci U S A 108(4):1561-6. [PubMed: 21220322]  [MGI Ref ID J:168246]

Calderon B; Carrero JA; Miller MJ; Unanue ER. 2011. Entry of diabetogenic T cells into islets induces changes that lead to amplification of the cellular response. Proc Natl Acad Sci U S A 108(4):1567-72. [PubMed: 21220309]  [MGI Ref ID J:168247]

Chattopadhyay G; Shevach EM. 2013. Antigen-specific induced T regulatory cells impair dendritic cell function via an IL-10/MARCH1-dependent mechanism. J Immunol 191(12):5875-84. [PubMed: 24218453]  [MGI Ref ID J:207135]

Dang Z; Kuffova L; Liu L; Forrester JV. 2014. Soluble antigen traffics rapidly and selectively from the corneal surface to the eye draining lymph node and activates T cells when codelivered with CpG oligonucleotides. J Leukoc Biol 95(3):431-40. [PubMed: 24295832]  [MGI Ref ID J:211878]

Danso-Abeam D; Staats KA; Franckaert D; Van Den Bosch L; Liston A; Gray DH; Dooley J. 2013. Aire mediates thymic expression and tolerance of pancreatic antigens via an unconventional transcriptional mechanism. Eur J Immunol 43(1):75-84. [PubMed: 23041971]  [MGI Ref ID J:191104]

DiPaolo RJ; Unanue ER. 2001. The level of peptide-MHC complex determines the susceptibility to autoimmune diabetes: studies in HEL transgenic mice. Eur J Immunol 31(12):3453-9. [PubMed: 11745364]  [MGI Ref ID J:73354]

Dugas V; Chabot-Roy G; Beauchamp C; Guimont-Desrochers F; Hillhouse EE; Liston A; Lesage S. 2013. Unusual selection and peripheral homeostasis for immunoregulatory CD4(-) CD8(-) T cells. Immunology 139(1):129-39. [PubMed: 23293940]  [MGI Ref ID J:198856]

Fournier S; Rathmell JC; Goodnow CC; Allison JP. 1997. T cell-mediated elimination of B7.2 transgenic B cells. Immunity 6(3):327-39. [PubMed: 9075933]  [MGI Ref ID J:112475]

Gosling KM; Makaroff LE; Theodoratos A; Kim YH; Whittle B; Rui L; Wu H; Hong NA; Kennedy GC; Fritz JA; Yates AL; Goodnow CC; Fahrer AM. 2007. A mutation in a chromosome condensin II subunit, kleisin beta, specifically disrupts T cell development. Proc Natl Acad Sci U S A 104(30):12445-50. [PubMed: 17640884]  [MGI Ref ID J:123300]

Guimont-Desrochers F; Beauchamp C; Chabot-Roy G; Dugas V; Hillhouse EE; Dusseault J; Langlois G; Gautier-Ethier P; Darwiche J; Sarfati M; Lesage S. 2009. Absence of CD47 in vivo influences thymic dendritic cell subset proportions but not negative selection of thymocytes. Int Immunol 21(2):167-77. [PubMed: 19147837]  [MGI Ref ID J:144491]

Hailman E; Allen PM. 2005. Inefficient cell spreading and cytoskeletal polarization by CD4+CD8+ thymocytes: regulation by the thymic environment. J Immunol 175(8):4847-57. [PubMed: 16210586]  [MGI Ref ID J:119055]

Ham DI; Kim SJ; Chen J; Vistica BP; Fariss RN; Lee RS; Wawrousek EF; Takase H; Yu CR; Egwuagu CE; Chan CC; Gery I. 2004. Central immunotolerance in transgenic mice expressing a foreign antigen under control of the rhodopsin promoter. Invest Ophthalmol Vis Sci 45(3):857-62. [PubMed: 14985302]  [MGI Ref ID J:131886]

Haribhai D; Engle D; Meyer M; Donermeyer D; White JM; Williams CB. 2003. A threshold for central T cell tolerance to an inducible serum protein. J Immunol 170(6):3007-14. [PubMed: 12626554]  [MGI Ref ID J:128816]

Holst J; Wang H; Eder KD; Workman CJ; Boyd KL; Baquet Z; Singh H; Forbes K; Chruscinski A; Smeyne R; van Oers NS; Utz PJ; Vignali DA. 2008. Scalable signaling mediated by T cell antigen receptor-CD3 ITAMs ensures effective negative selection and prevents autoimmunity. Nat Immunol 9(6):658-66. [PubMed: 18469818]  [MGI Ref ID J:136207]

Johnson AL; Aravind L; Shulzhenko N; Morgun A; Choi SY; Crockford TL; Lambe T; Domaschenz H; Kucharska EM; Zheng L; Vinuesa CG; Lenardo MJ; Goodnow CC; Cornall RJ; Schwartz RH. 2009. Themis is a member of a new metazoan gene family and is required for the completion of thymocyte positive selection. Nat Immunol 10(8):831-9. [PubMed: 19597497]  [MGI Ref ID J:151076]

Lambe T; Leung JC; Bouriez-Jones T; Silver K; Makinen K; Crockford TL; Ferry H; Forrester JV; Cornall RJ. 2006. CD4 T cell-dependent autoimmunity against a melanocyte neoantigen induces spontaneous vitiligo and depends upon Fas-Fas ligand interactions. J Immunol 177(5):3055-62. [PubMed: 16920942]  [MGI Ref ID J:139546]

Lambe T; Leung JC; Ferry H; Bouriez-Jones T; Makinen K; Crockford TL; Jiang HR; Nickerson JM; Peltonen L; Forrester JV; Cornall RJ. 2007. Limited peripheral T cell anergy predisposes to retinal autoimmunity. J Immunol 178(7):4276-83. [PubMed: 17371984]  [MGI Ref ID J:145054]

Lau AW; Biester S; Cornall RJ; Forrester JV. 2008. Lipopolysaccharide-Activated IL-10-Secreting Dendritic Cells Suppress Experimental Autoimmune Uveoretinitis by MHCII-Dependent Activation of CD62L-Expressing Regulatory T Cells. J Immunol 180(6):3889-99. [PubMed: 18322197]  [MGI Ref ID J:133030]

Liston A; Gray DH; Lesage S; Fletcher AL; Wilson J; Webster KE; Scott HS; Boyd RL; Peltonen L; Goodnow CC. 2004. Gene dosage--limiting role of Aire in thymic expression, clonal deletion, and organ-specific autoimmunity. J Exp Med 200(8):1015-26. [PubMed: 15492124]  [MGI Ref ID J:93912]

Liston A; Lesage S; Wilson J; Peltonen L; Goodnow CC. 2003. Aire regulates negative selection of organ-specific T cells. Nat Immunol 4(4):350-4. [PubMed: 12612579]  [MGI Ref ID J:128567]

Liston A; Siggs OM; Goodnow CC. 2007. Tracing the action of IL-2 in tolerance to islet-specific antigen. Immunol Cell Biol 85(4):338-42. [PubMed: 17372610]  [MGI Ref ID J:141735]

Lovitch SB; Esparza TJ; Schweitzer G; Herzog J; Unanue ER. 2007. Activation of type B T cells after protein immunization reveals novel pathways of in vivo presentation of peptides. J Immunol 178(1):122-33. [PubMed: 17182547]  [MGI Ref ID J:141945]

Nakagawa Y; Ohigashi I; Nitta T; Sakata M; Tanaka K; Murata S; Kanagawa O; Takahama Y. 2012. Thymic nurse cells provide microenvironment for secondary T cell receptor alpha rearrangement in cortical thymocytes. Proc Natl Acad Sci U S A 109(50):20572-7. [PubMed: 23188800]  [MGI Ref ID J:193119]

Nitta T; Nitta S; Lei Y; Lipp M; Takahama Y. 2009. CCR7-mediated migration of developing thymocytes to the medulla is essential for negative selection to tissue-restricted antigens. Proc Natl Acad Sci U S A 106(40):17129-33. [PubMed: 19805112]  [MGI Ref ID J:153692]

Pelletier N; McHeyzer-Williams LJ; Wong KA; Urich E; Fazilleau N; McHeyzer-Williams MG. 2010. Plasma cells negatively regulate the follicular helper T cell program. Nat Immunol 11(12):1110-8. [PubMed: 21037578]  [MGI Ref ID J:167325]

Pradhan S; Genebriera J; Denning WL; Felix K; Elmets CA; Timares L. 2006. CD4 T cell-induced, bid-dependent apoptosis of cutaneous dendritic cells regulates T cell expansion and immune responses. J Immunol 177(9):5956-67. [PubMed: 17056520]  [MGI Ref ID J:140532]

Quah BJ; Barlow VP; McPhun V; Matthaei KI; Hulett MD; Parish CR. 2008. Bystander B cells rapidly acquire antigen receptors from activated B cells by membrane transfer. Proc Natl Acad Sci U S A 105(11):4259-64. [PubMed: 18337504]  [MGI Ref ID J:173514]

Reichardt P; Patzak I; Jones K; Etemire E; Gunzer M; Hogg N. 2013. A role for LFA-1 in delaying T-lymphocyte egress from lymph nodes. EMBO J 32(6):829-43. [PubMed: 23443048]  [MGI Ref ID J:195308]

Rodriguez-Pinto D; Moreno J. 2005. B cells can prime naive CD4+ T cells in vivo in the absence of other professional antigen-presenting cells in a CD154-CD40-dependent manner. Eur J Immunol 35(4):1097-105. [PubMed: 15756646]  [MGI Ref ID J:97821]

Shi G; Cox CA; Vistica BP; Tan C; Wawrousek EF; Gery I. 2008. Phenotype switching by inflammation-inducing polarized Th17 cells, but not by Th1 cells. J Immunol 181(10):7205-13. [PubMed: 18981142]  [MGI Ref ID J:140933]

Shi G; Vistica BP; Nugent LF; Tan C; Wawrousek EF; Klinman DM; Gery I. 2013. Differential involvement of Th1 and Th17 in pathogenic autoimmune processes triggered by different TLR ligands. J Immunol 191(1):415-23. [PubMed: 23720812]  [MGI Ref ID J:205370]

Siggs OM; Miosge LA; Yates AL; Kucharska EM; Sheahan D; Brdicka T; Weiss A; Liston A; Goodnow CC. 2007. Opposing functions of the T cell receptor kinase ZAP-70 in immunity and tolerance differentially titrate in response to nucleotide substitutions. Immunity 27(6):912-26. [PubMed: 18093540]  [MGI Ref ID J:129169]

Silva DG; Daley SR; Hogan J; Lee SK; Teh CE; Hu DY; Lam KP; Goodnow CC; Vinuesa CG. 2011. Anti-islet autoantibodies trigger autoimmune diabetes in the presence of an increased frequency of islet-reactive CD4 T cells. Diabetes 60(8):2102-11. [PubMed: 21788582]  [MGI Ref ID J:186792]

Singh NJ; Bando JK; Schwartz RH. 2012. Subsets of Nonclonal Neighboring CD4(+) T Cells Specifically Regulate the Frequency of Individual Antigen-Reactive T Cells. Immunity 37(4):735-46. [PubMed: 23021952]  [MGI Ref ID J:188563]

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Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Mating System+/+ sibling x Hemizygote         (Female x Male)   17-MAY-08

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3300.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4290.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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